Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2748 and Pf1N1B4_2749 are separated by 75 nucleotides Pf1N1B4_2749 and Pf1N1B4_2750 are separated by 5 nucleotides Pf1N1B4_2750 and Pf1N1B4_2751 are separated by 178 nucleotides
Pf1N1B4_2748 - Transcriptional regulator, TetR family, at 3,088,303 to 3,088,980
_2748
Pf1N1B4_2749 - Quinate/shikimate 5-dehydrogenase I delta (EC 1.1.1.25), at 3,089,056 to 3,089,910
_2749
Pf1N1B4_2750 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 3,089,916 to 3,090,356
_2750
Pf1N1B4_2751 - Transcriptional regulator, LysR family, at 3,090,535 to 3,091,446
_2751
Position (kb)
3089
3090
3091 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3089.007 kb on - strand at 3089.226 kb on - strand, within Pf1N1B4_2749 at 3089.226 kb on - strand, within Pf1N1B4_2749 at 3089.226 kb on - strand, within Pf1N1B4_2749 at 3089.226 kb on - strand, within Pf1N1B4_2749 at 3089.314 kb on - strand, within Pf1N1B4_2749 at 3089.455 kb on + strand, within Pf1N1B4_2749 at 3089.455 kb on + strand, within Pf1N1B4_2749 at 3089.456 kb on - strand, within Pf1N1B4_2749 at 3089.456 kb on - strand, within Pf1N1B4_2749 at 3089.456 kb on - strand, within Pf1N1B4_2749 at 3089.456 kb on - strand, within Pf1N1B4_2749 at 3089.471 kb on - strand, within Pf1N1B4_2749 at 3089.515 kb on + strand, within Pf1N1B4_2749 at 3089.516 kb on - strand, within Pf1N1B4_2749 at 3089.516 kb on - strand, within Pf1N1B4_2749 at 3089.805 kb on - strand, within Pf1N1B4_2749 at 3090.286 kb on + strand, within Pf1N1B4_2750 at 3090.286 kb on + strand, within Pf1N1B4_2750 at 3090.286 kb on + strand, within Pf1N1B4_2750 at 3090.286 kb on + strand, within Pf1N1B4_2750 at 3090.286 kb on + strand, within Pf1N1B4_2750 at 3090.286 kb on + strand, within Pf1N1B4_2750 at 3090.371 kb on + strand at 3090.371 kb on + strand at 3090.372 kb on - strand at 3090.372 kb on - strand at 3090.372 kb on - strand at 3090.372 kb on - strand at 3090.374 kb on + strand at 3090.374 kb on + strand at 3090.374 kb on + strand at 3090.374 kb on + strand at 3090.375 kb on - strand at 3090.519 kb on + strand at 3090.519 kb on + strand at 3090.520 kb on - strand at 3090.520 kb on - strand at 3090.701 kb on + strand, within Pf1N1B4_2751 at 3090.701 kb on + strand, within Pf1N1B4_2751 at 3090.751 kb on + strand, within Pf1N1B4_2751 at 3090.751 kb on + strand, within Pf1N1B4_2751 at 3090.751 kb on + strand, within Pf1N1B4_2751 at 3090.752 kb on - strand, within Pf1N1B4_2751 at 3090.752 kb on - strand, within Pf1N1B4_2751 at 3090.752 kb on - strand, within Pf1N1B4_2751 at 3090.770 kb on + strand, within Pf1N1B4_2751 at 3090.770 kb on + strand, within Pf1N1B4_2751 at 3090.770 kb on + strand at 3090.770 kb on + strand, within Pf1N1B4_2751 at 3090.770 kb on + strand, within Pf1N1B4_2751 at 3090.770 kb on + strand, within Pf1N1B4_2751 at 3090.770 kb on + strand, within Pf1N1B4_2751 at 3090.770 kb on + strand, within Pf1N1B4_2751 at 3090.770 kb on + strand, within Pf1N1B4_2751 at 3090.770 kb on + strand, within Pf1N1B4_2751 at 3090.770 kb on + strand, within Pf1N1B4_2751 at 3090.771 kb on - strand, within Pf1N1B4_2751 at 3090.771 kb on - strand, within Pf1N1B4_2751 at 3090.771 kb on - strand, within Pf1N1B4_2751 at 3090.771 kb on - strand, within Pf1N1B4_2751 at 3090.771 kb on - strand, within Pf1N1B4_2751 at 3090.771 kb on - strand, within Pf1N1B4_2751 at 3090.771 kb on - strand, within Pf1N1B4_2751 at 3090.771 kb on - strand, within Pf1N1B4_2751 at 3090.771 kb on - strand, within Pf1N1B4_2751 at 3090.771 kb on - strand, within Pf1N1B4_2751 at 3090.771 kb on - strand, within Pf1N1B4_2751 at 3090.771 kb on - strand, within Pf1N1B4_2751 at 3090.771 kb on - strand, within Pf1N1B4_2751 at 3090.854 kb on + strand, within Pf1N1B4_2751 at 3090.918 kb on - strand, within Pf1N1B4_2751 at 3090.918 kb on - strand, within Pf1N1B4_2751 at 3091.099 kb on + strand, within Pf1N1B4_2751 at 3091.124 kb on + strand, within Pf1N1B4_2751 at 3091.124 kb on + strand, within Pf1N1B4_2751 at 3091.124 kb on + strand, within Pf1N1B4_2751 at 3091.124 kb on + strand, within Pf1N1B4_2751 at 3091.124 kb on + strand, within Pf1N1B4_2751 at 3091.124 kb on + strand, within Pf1N1B4_2751 at 3091.125 kb on - strand, within Pf1N1B4_2751 at 3091.125 kb on - strand, within Pf1N1B4_2751 at 3091.125 kb on - strand, within Pf1N1B4_2751 at 3091.125 kb on - strand, within Pf1N1B4_2751 at 3091.125 kb on - strand, within Pf1N1B4_2751 at 3091.235 kb on + strand, within Pf1N1B4_2751
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,089,007 - +0.3 3,089,226 - Pf1N1B4_2749 0.20 +1.2 3,089,226 - Pf1N1B4_2749 0.20 -0.1 3,089,226 - Pf1N1B4_2749 0.20 +0.5 3,089,226 - Pf1N1B4_2749 0.20 +0.6 3,089,314 - Pf1N1B4_2749 0.30 -0.5 3,089,455 + Pf1N1B4_2749 0.47 -0.9 3,089,455 + Pf1N1B4_2749 0.47 -0.2 3,089,456 - Pf1N1B4_2749 0.47 +0.4 3,089,456 - Pf1N1B4_2749 0.47 -1.4 3,089,456 - Pf1N1B4_2749 0.47 +0.4 3,089,456 - Pf1N1B4_2749 0.47 -0.8 3,089,471 - Pf1N1B4_2749 0.49 -0.5 3,089,515 + Pf1N1B4_2749 0.54 -1.6 3,089,516 - Pf1N1B4_2749 0.54 +0.7 3,089,516 - Pf1N1B4_2749 0.54 +0.0 3,089,805 - Pf1N1B4_2749 0.88 -2.4 3,090,286 + Pf1N1B4_2750 0.84 +0.4 3,090,286 + Pf1N1B4_2750 0.84 +0.0 3,090,286 + Pf1N1B4_2750 0.84 -1.0 3,090,286 + Pf1N1B4_2750 0.84 -0.6 3,090,286 + Pf1N1B4_2750 0.84 -0.8 3,090,286 + Pf1N1B4_2750 0.84 -0.5 3,090,371 + +0.3 3,090,371 + -1.0 3,090,372 - +0.6 3,090,372 - +0.2 3,090,372 - -0.4 3,090,372 - -1.0 3,090,374 + +0.4 3,090,374 + +1.2 3,090,374 + +1.2 3,090,374 + +2.0 3,090,375 - +0.2 3,090,519 + +0.2 3,090,519 + -0.5 3,090,520 - +0.8 3,090,520 - -0.4 3,090,701 + Pf1N1B4_2751 0.18 +0.4 3,090,701 + Pf1N1B4_2751 0.18 -0.2 3,090,751 + Pf1N1B4_2751 0.24 -0.1 3,090,751 + Pf1N1B4_2751 0.24 -0.8 3,090,751 + Pf1N1B4_2751 0.24 -0.6 3,090,752 - Pf1N1B4_2751 0.24 +1.2 3,090,752 - Pf1N1B4_2751 0.24 -1.1 3,090,752 - Pf1N1B4_2751 0.24 +0.0 3,090,770 + Pf1N1B4_2751 0.26 +0.4 3,090,770 + Pf1N1B4_2751 0.26 +0.8 3,090,770 + +1.2 3,090,770 + Pf1N1B4_2751 0.26 +0.6 3,090,770 + Pf1N1B4_2751 0.26 +0.5 3,090,770 + Pf1N1B4_2751 0.26 +0.5 3,090,770 + Pf1N1B4_2751 0.26 -0.4 3,090,770 + Pf1N1B4_2751 0.26 +1.4 3,090,770 + Pf1N1B4_2751 0.26 -0.6 3,090,770 + Pf1N1B4_2751 0.26 -0.2 3,090,770 + Pf1N1B4_2751 0.26 -0.8 3,090,771 - Pf1N1B4_2751 0.26 +0.7 3,090,771 - Pf1N1B4_2751 0.26 +0.9 3,090,771 - Pf1N1B4_2751 0.26 +0.6 3,090,771 - Pf1N1B4_2751 0.26 -0.6 3,090,771 - Pf1N1B4_2751 0.26 +2.8 3,090,771 - Pf1N1B4_2751 0.26 +1.2 3,090,771 - Pf1N1B4_2751 0.26 -1.1 3,090,771 - Pf1N1B4_2751 0.26 +0.9 3,090,771 - Pf1N1B4_2751 0.26 -0.4 3,090,771 - Pf1N1B4_2751 0.26 -0.2 3,090,771 - Pf1N1B4_2751 0.26 +0.6 3,090,771 - Pf1N1B4_2751 0.26 +0.2 3,090,771 - Pf1N1B4_2751 0.26 +1.3 3,090,854 + Pf1N1B4_2751 0.35 -0.1 3,090,918 - Pf1N1B4_2751 0.42 +0.5 3,090,918 - Pf1N1B4_2751 0.42 +2.2 3,091,099 + Pf1N1B4_2751 0.62 +0.4 3,091,124 + Pf1N1B4_2751 0.65 +1.2 3,091,124 + Pf1N1B4_2751 0.65 +0.6 3,091,124 + Pf1N1B4_2751 0.65 -0.2 3,091,124 + Pf1N1B4_2751 0.65 +0.6 3,091,124 + Pf1N1B4_2751 0.65 -0.4 3,091,124 + Pf1N1B4_2751 0.65 +1.0 3,091,125 - Pf1N1B4_2751 0.65 +0.2 3,091,125 - Pf1N1B4_2751 0.65 -0.2 3,091,125 - Pf1N1B4_2751 0.65 -0.4 3,091,125 - Pf1N1B4_2751 0.65 -0.3 3,091,125 - Pf1N1B4_2751 0.65 +0.4 3,091,235 + Pf1N1B4_2751 0.77 +0.2
Or see this region's nucleotide sequence