Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2490 and Pf1N1B4_2491 overlap by 4 nucleotides Pf1N1B4_2491 and Pf1N1B4_2492 are separated by 120 nucleotides Pf1N1B4_2492 and Pf1N1B4_2493 are separated by 277 nucleotides
Pf1N1B4_2490 - Biotin synthesis protein BioC, at 2,823,076 to 2,823,888
_2490
Pf1N1B4_2491 - Dethiobiotin synthetase (EC 6.3.3.3), at 2,823,885 to 2,824,565
_2491
Pf1N1B4_2492 - FIG00954771: hypothetical protein, at 2,824,686 to 2,824,982
_2492
Pf1N1B4_2493 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 2,825,260 to 2,827,065
_2493
Position (kb)
2824
2825 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2823.694 kb on + strand, within Pf1N1B4_2490 at 2823.811 kb on + strand at 2823.811 kb on + strand at 2823.812 kb on - strand at 2823.812 kb on - strand at 2823.812 kb on - strand at 2823.850 kb on + strand at 2823.850 kb on + strand at 2823.850 kb on + strand at 2823.851 kb on - strand at 2823.884 kb on + strand at 2823.885 kb on - strand at 2823.885 kb on - strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.898 kb on - strand at 2823.898 kb on - strand at 2823.898 kb on - strand at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.583 kb on + strand at 2824.583 kb on + strand at 2824.583 kb on + strand at 2824.730 kb on + strand, within Pf1N1B4_2492 at 2824.730 kb on + strand, within Pf1N1B4_2492 at 2824.880 kb on + strand, within Pf1N1B4_2492 at 2824.880 kb on + strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.907 kb on - strand, within Pf1N1B4_2492 at 2824.907 kb on - strand at 2824.970 kb on + strand at 2824.971 kb on - strand at 2824.971 kb on - strand at 2825.038 kb on + strand at 2825.038 kb on + strand at 2825.038 kb on + strand at 2825.039 kb on - strand at 2825.039 kb on - strand at 2825.039 kb on - strand at 2825.057 kb on + strand at 2825.057 kb on + strand at 2825.058 kb on - strand at 2825.058 kb on - strand at 2825.058 kb on - strand at 2825.058 kb on - strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.178 kb on + strand at 2825.178 kb on + strand at 2825.179 kb on - strand at 2825.179 kb on - strand at 2825.179 kb on - strand at 2825.237 kb on + strand at 2825.237 kb on + strand at 2825.260 kb on - strand at 2825.290 kb on - strand at 2825.506 kb on + strand, within Pf1N1B4_2493 at 2825.507 kb on - strand, within Pf1N1B4_2493 at 2825.552 kb on + strand, within Pf1N1B4_2493 at 2825.707 kb on + strand, within Pf1N1B4_2493 at 2825.824 kb on + strand, within Pf1N1B4_2493
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,823,694 + Pf1N1B4_2490 0.76 -1.1 2,823,811 + -0.8 2,823,811 + -0.8 2,823,812 - -0.8 2,823,812 - -0.7 2,823,812 - +0.2 2,823,850 + -1.1 2,823,850 + -0.1 2,823,850 + +0.1 2,823,851 - +0.6 2,823,884 + -0.6 2,823,885 - +0.8 2,823,885 - +0.8 2,823,897 + +2.2 2,823,897 + -0.9 2,823,897 + +0.1 2,823,897 + +0.4 2,823,897 + -1.8 2,823,898 - -0.8 2,823,898 - +0.5 2,823,898 - -1.6 2,824,089 + Pf1N1B4_2491 0.30 +0.1 2,824,089 + Pf1N1B4_2491 0.30 -0.1 2,824,089 + Pf1N1B4_2491 0.30 +0.2 2,824,089 + Pf1N1B4_2491 0.30 -1.3 2,824,090 - Pf1N1B4_2491 0.30 -1.6 2,824,090 - Pf1N1B4_2491 0.30 +1.7 2,824,090 - Pf1N1B4_2491 0.30 -0.8 2,824,090 - Pf1N1B4_2491 0.30 -0.5 2,824,090 - Pf1N1B4_2491 0.30 -0.8 2,824,583 + -0.4 2,824,583 + +0.2 2,824,583 + +1.7 2,824,730 + Pf1N1B4_2492 0.15 -0.3 2,824,730 + Pf1N1B4_2492 0.15 -1.3 2,824,880 + Pf1N1B4_2492 0.65 -0.8 2,824,880 + Pf1N1B4_2492 0.65 -1.1 2,824,881 - Pf1N1B4_2492 0.66 -0.2 2,824,881 - Pf1N1B4_2492 0.66 +1.2 2,824,881 - Pf1N1B4_2492 0.66 -2.5 2,824,906 + Pf1N1B4_2492 0.74 -0.9 2,824,906 + Pf1N1B4_2492 0.74 +1.0 2,824,906 + Pf1N1B4_2492 0.74 -0.8 2,824,906 + Pf1N1B4_2492 0.74 -0.8 2,824,906 + Pf1N1B4_2492 0.74 +1.2 2,824,907 - Pf1N1B4_2492 0.74 -2.7 2,824,907 - -0.8 2,824,970 + -0.0 2,824,971 - +0.2 2,824,971 - +1.5 2,825,038 + +0.2 2,825,038 + -1.1 2,825,038 + -0.5 2,825,039 - +1.1 2,825,039 - +0.3 2,825,039 - -0.6 2,825,057 + -0.7 2,825,057 + +0.7 2,825,058 - -1.3 2,825,058 - +0.4 2,825,058 - +0.7 2,825,058 - -2.2 2,825,067 + +0.2 2,825,067 + -0.8 2,825,067 + -0.3 2,825,067 + -0.1 2,825,067 + -0.8 2,825,068 - -0.3 2,825,068 - -1.1 2,825,068 - +0.9 2,825,068 - +1.1 2,825,068 - -0.6 2,825,178 + -0.3 2,825,178 + -0.3 2,825,179 - +0.2 2,825,179 - -0.6 2,825,179 - +0.2 2,825,237 + +0.4 2,825,237 + +0.8 2,825,260 - -0.1 2,825,290 - +2.8 2,825,506 + Pf1N1B4_2493 0.14 +0.8 2,825,507 - Pf1N1B4_2493 0.14 +1.2 2,825,552 + Pf1N1B4_2493 0.16 -0.3 2,825,707 + Pf1N1B4_2493 0.25 +0.2 2,825,824 + Pf1N1B4_2493 0.31 +1.9
Or see this region's nucleotide sequence