Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_199 and Pf1N1B4_200 are separated by 35 nucleotides Pf1N1B4_200 and Pf1N1B4_201 overlap by 4 nucleotides Pf1N1B4_201 and Pf1N1B4_202 overlap by 1 nucleotides Pf1N1B4_202 and Pf1N1B4_203 are separated by 0 nucleotides
Pf1N1B4_199 - FIG049111: Hypothetical protein in pyoverdin gene cluster, at 246,629 to 247,204
_199
Pf1N1B4_200 - FIG014801: Cation ABC transporter, periplasmic cation-binding protein, at 247,240 to 248,169
_200
Pf1N1B4_201 - ABC transporter in pyoverdin gene cluster, ATP-binding component, at 248,166 to 249,017
_201
Pf1N1B4_202 - ABC transporter in pyoverdin gene cluster, permease component, at 249,017 to 249,916
_202
Pf1N1B4_203 - ABC transporter in pyoverdin gene cluster, periplasmic component, at 249,917 to 250,888
_203
Position (kb)
248
249
250 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 247.245 kb on + strand at 247.246 kb on - strand at 247.246 kb on - strand at 247.246 kb on - strand at 247.246 kb on - strand at 247.369 kb on + strand at 247.369 kb on + strand, within Pf1N1B4_200 at 247.370 kb on - strand, within Pf1N1B4_200 at 247.372 kb on + strand, within Pf1N1B4_200 at 247.372 kb on + strand, within Pf1N1B4_200 at 247.372 kb on + strand, within Pf1N1B4_200 at 247.372 kb on + strand, within Pf1N1B4_200 at 247.373 kb on - strand, within Pf1N1B4_200 at 247.373 kb on - strand, within Pf1N1B4_200 at 247.373 kb on - strand, within Pf1N1B4_200 at 247.373 kb on - strand, within Pf1N1B4_200 at 247.373 kb on - strand, within Pf1N1B4_200 at 247.378 kb on + strand, within Pf1N1B4_200 at 247.378 kb on + strand, within Pf1N1B4_200 at 247.378 kb on + strand, within Pf1N1B4_200 at 247.378 kb on + strand, within Pf1N1B4_200 at 247.379 kb on - strand, within Pf1N1B4_200 at 247.379 kb on - strand, within Pf1N1B4_200 at 247.379 kb on - strand, within Pf1N1B4_200 at 247.379 kb on - strand, within Pf1N1B4_200 at 247.451 kb on - strand, within Pf1N1B4_200 at 247.451 kb on - strand, within Pf1N1B4_200 at 247.502 kb on + strand, within Pf1N1B4_200 at 247.502 kb on + strand, within Pf1N1B4_200 at 247.506 kb on + strand, within Pf1N1B4_200 at 247.744 kb on + strand, within Pf1N1B4_200 at 247.867 kb on + strand, within Pf1N1B4_200 at 247.868 kb on - strand, within Pf1N1B4_200 at 248.020 kb on + strand, within Pf1N1B4_200 at 248.020 kb on + strand, within Pf1N1B4_200 at 248.021 kb on - strand, within Pf1N1B4_200 at 248.524 kb on - strand, within Pf1N1B4_201 at 248.558 kb on - strand, within Pf1N1B4_201 at 248.624 kb on + strand, within Pf1N1B4_201 at 248.625 kb on - strand, within Pf1N1B4_201 at 248.632 kb on - strand, within Pf1N1B4_201 at 249.023 kb on + strand at 249.024 kb on - strand at 249.251 kb on + strand at 249.251 kb on + strand, within Pf1N1B4_202 at 249.252 kb on - strand at 249.264 kb on - strand at 249.311 kb on + strand, within Pf1N1B4_202 at 249.311 kb on + strand at 249.311 kb on + strand at 249.311 kb on + strand, within Pf1N1B4_202 at 249.311 kb on + strand, within Pf1N1B4_202 at 249.312 kb on - strand, within Pf1N1B4_202 at 249.315 kb on + strand, within Pf1N1B4_202 at 249.315 kb on + strand, within Pf1N1B4_202 at 249.316 kb on - strand, within Pf1N1B4_202 at 249.316 kb on - strand, within Pf1N1B4_202 at 249.326 kb on - strand, within Pf1N1B4_202 at 249.533 kb on + strand, within Pf1N1B4_202 at 249.533 kb on + strand, within Pf1N1B4_202 at 249.534 kb on - strand, within Pf1N1B4_202 at 249.608 kb on + strand, within Pf1N1B4_202
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 247,245 + -0.8 247,246 - +0.4 247,246 - -1.2 247,246 - +0.6 247,246 - +0.4 247,369 + +1.8 247,369 + Pf1N1B4_200 0.14 -1.8 247,370 - Pf1N1B4_200 0.14 +0.8 247,372 + Pf1N1B4_200 0.14 -0.6 247,372 + Pf1N1B4_200 0.14 -0.7 247,372 + Pf1N1B4_200 0.14 -0.7 247,372 + Pf1N1B4_200 0.14 +0.2 247,373 - Pf1N1B4_200 0.14 -0.8 247,373 - Pf1N1B4_200 0.14 -0.8 247,373 - Pf1N1B4_200 0.14 +0.8 247,373 - Pf1N1B4_200 0.14 +0.0 247,373 - Pf1N1B4_200 0.14 -1.2 247,378 + Pf1N1B4_200 0.15 +2.1 247,378 + Pf1N1B4_200 0.15 -1.0 247,378 + Pf1N1B4_200 0.15 +1.4 247,378 + Pf1N1B4_200 0.15 +0.2 247,379 - Pf1N1B4_200 0.15 +0.5 247,379 - Pf1N1B4_200 0.15 -0.6 247,379 - Pf1N1B4_200 0.15 -0.2 247,379 - Pf1N1B4_200 0.15 +2.8 247,451 - Pf1N1B4_200 0.23 -0.5 247,451 - Pf1N1B4_200 0.23 -1.2 247,502 + Pf1N1B4_200 0.28 +0.1 247,502 + Pf1N1B4_200 0.28 +0.3 247,506 + Pf1N1B4_200 0.29 +0.8 247,744 + Pf1N1B4_200 0.54 +0.2 247,867 + Pf1N1B4_200 0.67 -0.8 247,868 - Pf1N1B4_200 0.68 +0.0 248,020 + Pf1N1B4_200 0.84 +0.2 248,020 + Pf1N1B4_200 0.84 -0.6 248,021 - Pf1N1B4_200 0.84 -2.0 248,524 - Pf1N1B4_201 0.42 +0.2 248,558 - Pf1N1B4_201 0.46 -1.5 248,624 + Pf1N1B4_201 0.54 -0.2 248,625 - Pf1N1B4_201 0.54 -0.8 248,632 - Pf1N1B4_201 0.55 -0.5 249,023 + -1.2 249,024 - -0.7 249,251 + +0.5 249,251 + Pf1N1B4_202 0.26 -0.6 249,252 - +0.8 249,264 - +1.5 249,311 + Pf1N1B4_202 0.33 +0.6 249,311 + +0.2 249,311 + +0.8 249,311 + Pf1N1B4_202 0.33 +0.2 249,311 + Pf1N1B4_202 0.33 -0.8 249,312 - Pf1N1B4_202 0.33 +0.1 249,315 + Pf1N1B4_202 0.33 +0.8 249,315 + Pf1N1B4_202 0.33 -0.9 249,316 - Pf1N1B4_202 0.33 +0.6 249,316 - Pf1N1B4_202 0.33 -0.9 249,326 - Pf1N1B4_202 0.34 -0.5 249,533 + Pf1N1B4_202 0.57 -0.4 249,533 + Pf1N1B4_202 0.57 +1.5 249,534 - Pf1N1B4_202 0.57 -1.5 249,608 + Pf1N1B4_202 0.66 -0.2
Or see this region's nucleotide sequence