Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1543 and Pf1N1B4_1544 are separated by 215 nucleotides Pf1N1B4_1544 and Pf1N1B4_1545 are separated by 251 nucleotides
Pf1N1B4_1543 - Type IV pilus biogenesis protein PilM, at 1,778,680 to 1,779,744
_1543
Pf1N1B4_1544 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 1,779,960 to 1,782,407
_1544
Pf1N1B4_1545 - NADP-dependent malic enzyme (EC 1.1.1.40), at 1,782,659 to 1,783,927
_1545
Position (kb)
1779
1780
1781
1782
1783 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1778.979 kb on - strand, within Pf1N1B4_1543 at 1779.038 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.096 kb on - strand, within Pf1N1B4_1543 at 1779.096 kb on - strand, within Pf1N1B4_1543 at 1779.227 kb on + strand, within Pf1N1B4_1543 at 1779.228 kb on - strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.405 kb on - strand, within Pf1N1B4_1543 at 1779.405 kb on - strand, within Pf1N1B4_1543 at 1779.585 kb on + strand, within Pf1N1B4_1543 at 1779.629 kb on + strand, within Pf1N1B4_1543 at 1779.630 kb on - strand, within Pf1N1B4_1543 at 1779.664 kb on + strand at 1779.664 kb on + strand at 1779.665 kb on - strand at 1779.756 kb on + strand at 1779.854 kb on + strand at 1779.854 kb on + strand at 1779.854 kb on + strand at 1779.854 kb on + strand at 1779.854 kb on + strand at 1779.856 kb on + strand at 1779.856 kb on + strand at 1779.856 kb on + strand at 1779.856 kb on + strand at 1779.906 kb on + strand at 1779.927 kb on + strand at 1780.085 kb on + strand at 1780.210 kb on + strand at 1780.301 kb on + strand, within Pf1N1B4_1544 at 1780.449 kb on + strand at 1780.464 kb on + strand, within Pf1N1B4_1544 at 1780.509 kb on + strand, within Pf1N1B4_1544 at 1780.509 kb on + strand, within Pf1N1B4_1544 at 1780.651 kb on - strand, within Pf1N1B4_1544 at 1780.651 kb on - strand, within Pf1N1B4_1544 at 1780.651 kb on - strand, within Pf1N1B4_1544 at 1780.652 kb on + strand, within Pf1N1B4_1544 at 1780.680 kb on + strand, within Pf1N1B4_1544 at 1780.717 kb on - strand at 1780.717 kb on - strand, within Pf1N1B4_1544 at 1780.749 kb on + strand, within Pf1N1B4_1544 at 1780.749 kb on + strand, within Pf1N1B4_1544 at 1780.803 kb on + strand, within Pf1N1B4_1544 at 1781.310 kb on + strand, within Pf1N1B4_1544 at 1781.310 kb on + strand, within Pf1N1B4_1544 at 1781.310 kb on + strand, within Pf1N1B4_1544 at 1781.311 kb on - strand, within Pf1N1B4_1544 at 1781.311 kb on - strand, within Pf1N1B4_1544 at 1781.311 kb on - strand, within Pf1N1B4_1544 at 1781.311 kb on - strand, within Pf1N1B4_1544 at 1781.311 kb on - strand, within Pf1N1B4_1544 at 1781.311 kb on - strand, within Pf1N1B4_1544 at 1781.392 kb on - strand, within Pf1N1B4_1544 at 1781.393 kb on + strand, within Pf1N1B4_1544 at 1781.423 kb on + strand, within Pf1N1B4_1544 at 1781.424 kb on - strand, within Pf1N1B4_1544 at 1781.424 kb on - strand, within Pf1N1B4_1544 at 1781.424 kb on - strand, within Pf1N1B4_1544 at 1781.518 kb on - strand, within Pf1N1B4_1544 at 1781.583 kb on + strand, within Pf1N1B4_1544 at 1781.584 kb on - strand, within Pf1N1B4_1544 at 1781.584 kb on - strand, within Pf1N1B4_1544 at 1781.706 kb on + strand, within Pf1N1B4_1544 at 1781.721 kb on + strand, within Pf1N1B4_1544 at 1781.762 kb on - strand at 1781.762 kb on - strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1782.066 kb on + strand, within Pf1N1B4_1544 at 1782.079 kb on - strand, within Pf1N1B4_1544 at 1782.079 kb on - strand, within Pf1N1B4_1544 at 1782.171 kb on + strand at 1782.171 kb on + strand at 1782.172 kb on - strand at 1782.172 kb on - strand at 1782.172 kb on - strand at 1782.172 kb on - strand at 1782.291 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.414 kb on - strand at 1782.419 kb on + strand at 1782.419 kb on + strand at 1782.420 kb on - strand at 1782.420 kb on - strand at 1782.423 kb on + strand at 1782.423 kb on + strand at 1782.423 kb on + strand at 1782.424 kb on - strand at 1782.424 kb on - strand at 1782.424 kb on - strand at 1782.424 kb on - strand at 1782.593 kb on + strand at 1782.594 kb on - strand at 1782.696 kb on + strand at 1782.697 kb on - strand at 1782.697 kb on - strand at 1782.697 kb on - strand at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.837 kb on + strand, within Pf1N1B4_1545 at 1782.974 kb on + strand, within Pf1N1B4_1545 at 1782.974 kb on + strand, within Pf1N1B4_1545 at 1782.974 kb on + strand, within Pf1N1B4_1545 at 1782.975 kb on - strand, within Pf1N1B4_1545 at 1782.975 kb on - strand, within Pf1N1B4_1545 at 1782.975 kb on - strand, within Pf1N1B4_1545 at 1783.227 kb on + strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,778,979 - Pf1N1B4_1543 0.28 +1.6 1,779,038 + Pf1N1B4_1543 0.34 -1.3 1,779,095 + Pf1N1B4_1543 0.39 +0.0 1,779,095 + Pf1N1B4_1543 0.39 -0.3 1,779,095 + Pf1N1B4_1543 0.39 -0.3 1,779,095 + Pf1N1B4_1543 0.39 +0.3 1,779,095 + Pf1N1B4_1543 0.39 -0.6 1,779,095 + Pf1N1B4_1543 0.39 -0.5 1,779,096 - Pf1N1B4_1543 0.39 -0.3 1,779,096 - Pf1N1B4_1543 0.39 +3.3 1,779,227 + Pf1N1B4_1543 0.51 +0.6 1,779,228 - Pf1N1B4_1543 0.51 -1.0 1,779,404 + Pf1N1B4_1543 0.68 -1.1 1,779,404 + Pf1N1B4_1543 0.68 +1.3 1,779,404 + Pf1N1B4_1543 0.68 -1.3 1,779,404 + Pf1N1B4_1543 0.68 -0.5 1,779,404 + Pf1N1B4_1543 0.68 +1.0 1,779,404 + Pf1N1B4_1543 0.68 -0.2 1,779,404 + Pf1N1B4_1543 0.68 -1.0 1,779,405 - Pf1N1B4_1543 0.68 -0.7 1,779,405 - Pf1N1B4_1543 0.68 +0.7 1,779,585 + Pf1N1B4_1543 0.85 +0.5 1,779,629 + Pf1N1B4_1543 0.89 +0.2 1,779,630 - Pf1N1B4_1543 0.89 +2.3 1,779,664 + -0.3 1,779,664 + -0.8 1,779,665 - +0.9 1,779,756 + -0.5 1,779,854 + +0.6 1,779,854 + +1.1 1,779,854 + +1.3 1,779,854 + -0.9 1,779,854 + +1.3 1,779,856 + +0.7 1,779,856 + -0.8 1,779,856 + -0.1 1,779,856 + -2.3 1,779,906 + +0.2 1,779,927 + -1.0 1,780,085 + +0.7 1,780,210 + +0.7 1,780,301 + Pf1N1B4_1544 0.14 +0.3 1,780,449 + +1.3 1,780,464 + Pf1N1B4_1544 0.21 -0.3 1,780,509 + Pf1N1B4_1544 0.22 -0.5 1,780,509 + Pf1N1B4_1544 0.22 -1.8 1,780,651 - Pf1N1B4_1544 0.28 -0.5 1,780,651 - Pf1N1B4_1544 0.28 -0.3 1,780,651 - Pf1N1B4_1544 0.28 -1.8 1,780,652 + Pf1N1B4_1544 0.28 +0.2 1,780,680 + Pf1N1B4_1544 0.29 +0.9 1,780,717 - +0.3 1,780,717 - Pf1N1B4_1544 0.31 -0.7 1,780,749 + Pf1N1B4_1544 0.32 -1.0 1,780,749 + Pf1N1B4_1544 0.32 +0.3 1,780,803 + Pf1N1B4_1544 0.34 +0.0 1,781,310 + Pf1N1B4_1544 0.55 -1.3 1,781,310 + Pf1N1B4_1544 0.55 -1.7 1,781,310 + Pf1N1B4_1544 0.55 -1.0 1,781,311 - Pf1N1B4_1544 0.55 +1.3 1,781,311 - Pf1N1B4_1544 0.55 -1.0 1,781,311 - Pf1N1B4_1544 0.55 +1.3 1,781,311 - Pf1N1B4_1544 0.55 +1.3 1,781,311 - Pf1N1B4_1544 0.55 +0.7 1,781,311 - Pf1N1B4_1544 0.55 +0.5 1,781,392 - Pf1N1B4_1544 0.58 +0.1 1,781,393 + Pf1N1B4_1544 0.59 +1.0 1,781,423 + Pf1N1B4_1544 0.60 -1.3 1,781,424 - Pf1N1B4_1544 0.60 +0.3 1,781,424 - Pf1N1B4_1544 0.60 -0.7 1,781,424 - Pf1N1B4_1544 0.60 -1.0 1,781,518 - Pf1N1B4_1544 0.64 +0.0 1,781,583 + Pf1N1B4_1544 0.66 -1.4 1,781,584 - Pf1N1B4_1544 0.66 +1.3 1,781,584 - Pf1N1B4_1544 0.66 +0.3 1,781,706 + Pf1N1B4_1544 0.71 -0.3 1,781,721 + Pf1N1B4_1544 0.72 -0.1 1,781,762 - +1.3 1,781,762 - Pf1N1B4_1544 0.74 -0.5 1,781,934 + Pf1N1B4_1544 0.81 -0.3 1,781,934 + Pf1N1B4_1544 0.81 -1.0 1,781,934 + Pf1N1B4_1544 0.81 -0.4 1,781,934 + Pf1N1B4_1544 0.81 +0.6 1,781,934 + Pf1N1B4_1544 0.81 -0.7 1,781,934 + Pf1N1B4_1544 0.81 -0.3 1,781,934 + Pf1N1B4_1544 0.81 -0.1 1,781,934 + Pf1N1B4_1544 0.81 -1.1 1,781,934 + Pf1N1B4_1544 0.81 +1.3 1,781,935 - Pf1N1B4_1544 0.81 -0.3 1,781,935 - Pf1N1B4_1544 0.81 -0.4 1,781,935 - Pf1N1B4_1544 0.81 -1.3 1,781,935 - -0.3 1,781,935 - Pf1N1B4_1544 0.81 +0.7 1,781,935 - Pf1N1B4_1544 0.81 +0.0 1,781,935 - Pf1N1B4_1544 0.81 -0.8 1,781,935 - Pf1N1B4_1544 0.81 -0.8 1,781,935 - Pf1N1B4_1544 0.81 +0.9 1,781,935 - Pf1N1B4_1544 0.81 -1.0 1,781,935 - Pf1N1B4_1544 0.81 -1.5 1,782,066 + Pf1N1B4_1544 0.86 -1.7 1,782,079 - Pf1N1B4_1544 0.87 -1.7 1,782,079 - Pf1N1B4_1544 0.87 +1.3 1,782,171 + -2.3 1,782,171 + +0.0 1,782,172 - +0.7 1,782,172 - -1.0 1,782,172 - -1.0 1,782,172 - +0.2 1,782,291 + +0.7 1,782,413 + -0.1 1,782,413 + -0.5 1,782,413 + -0.8 1,782,413 + +0.3 1,782,413 + -0.4 1,782,414 - -0.8 1,782,419 + +0.6 1,782,419 + -0.1 1,782,420 - +1.5 1,782,420 - +1.0 1,782,423 + -0.3 1,782,423 + -0.1 1,782,423 + +0.4 1,782,424 - +1.3 1,782,424 - +0.2 1,782,424 - +0.1 1,782,424 - +0.3 1,782,593 + +0.7 1,782,594 - +0.1 1,782,696 + -0.3 1,782,697 - -0.3 1,782,697 - +1.8 1,782,697 - +1.0 1,782,804 + Pf1N1B4_1545 0.11 +0.9 1,782,804 + Pf1N1B4_1545 0.11 +0.6 1,782,804 + Pf1N1B4_1545 0.11 -0.3 1,782,804 + Pf1N1B4_1545 0.11 -1.2 1,782,804 + Pf1N1B4_1545 0.11 +0.3 1,782,804 + Pf1N1B4_1545 0.11 +0.7 1,782,804 + Pf1N1B4_1545 0.11 -1.0 1,782,805 - Pf1N1B4_1545 0.12 +0.3 1,782,805 - Pf1N1B4_1545 0.12 +1.3 1,782,805 - Pf1N1B4_1545 0.12 -0.5 1,782,805 - Pf1N1B4_1545 0.12 -0.1 1,782,805 - Pf1N1B4_1545 0.12 -1.7 1,782,805 - Pf1N1B4_1545 0.12 +0.2 1,782,805 - Pf1N1B4_1545 0.12 -0.7 1,782,805 - Pf1N1B4_1545 0.12 +0.3 1,782,837 + Pf1N1B4_1545 0.14 -0.7 1,782,974 + Pf1N1B4_1545 0.25 +0.6 1,782,974 + Pf1N1B4_1545 0.25 -0.3 1,782,974 + Pf1N1B4_1545 0.25 +0.6 1,782,975 - Pf1N1B4_1545 0.25 +0.7 1,782,975 - Pf1N1B4_1545 0.25 +1.0 1,782,975 - Pf1N1B4_1545 0.25 -0.3 1,783,227 + Pf1N1B4_1545 0.45 -0.7 1,783,228 - Pf1N1B4_1545 0.45 -0.3 1,783,228 - Pf1N1B4_1545 0.45 +1.1 1,783,228 - Pf1N1B4_1545 0.45 +0.3 1,783,281 + Pf1N1B4_1545 0.49 +1.3 1,783,281 + Pf1N1B4_1545 0.49 -0.5 1,783,281 + Pf1N1B4_1545 0.49 -2.1 1,783,281 + Pf1N1B4_1545 0.49 +0.3 1,783,281 + Pf1N1B4_1545 0.49 -0.3 1,783,281 + +1.3 1,783,281 + Pf1N1B4_1545 0.49 +0.5 1,783,281 + Pf1N1B4_1545 0.49 +0.6 1,783,282 - Pf1N1B4_1545 0.49 -0.4 1,783,282 - Pf1N1B4_1545 0.49 -0.4 1,783,282 - Pf1N1B4_1545 0.49 -0.7 1,783,282 - Pf1N1B4_1545 0.49 -0.7 1,783,282 - Pf1N1B4_1545 0.49 +1.7 1,783,282 - Pf1N1B4_1545 0.49 -0.7 1,783,282 - Pf1N1B4_1545 0.49 -0.4 1,783,282 - Pf1N1B4_1545 0.49 +1.1 1,783,282 - Pf1N1B4_1545 0.49 -1.3 1,783,282 - Pf1N1B4_1545 0.49 +0.7 1,783,282 - Pf1N1B4_1545 0.49 +0.9 1,783,282 - Pf1N1B4_1545 0.49 -0.7 1,783,282 - Pf1N1B4_1545 0.49 +0.0 1,783,282 - Pf1N1B4_1545 0.49 -0.5 1,783,282 - Pf1N1B4_1545 0.49 +1.8 1,783,282 - Pf1N1B4_1545 0.49 +0.3
Or see this region's nucleotide sequence