Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1227 and Pf1N1B4_1228 are separated by 245 nucleotides Pf1N1B4_1228 and Pf1N1B4_1229 are separated by 10 nucleotides
Pf1N1B4_1227 - FIG00954870: hypothetical protein, at 1,393,478 to 1,393,822
_1227
Pf1N1B4_1228 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31), at 1,394,068 to 1,394,955
_1228
Pf1N1B4_1229 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (from data) , at 1,394,966 to 1,396,483
_1229
Position (kb)
1394
1395 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1393.088 kb on + strand at 1393.144 kb on + strand at 1393.144 kb on + strand at 1393.181 kb on + strand at 1393.182 kb on - strand at 1393.188 kb on + strand at 1393.189 kb on - strand at 1393.214 kb on - strand at 1393.225 kb on + strand at 1393.227 kb on + strand at 1393.227 kb on + strand at 1393.227 kb on + strand at 1393.228 kb on - strand at 1393.228 kb on - strand at 1393.230 kb on + strand at 1393.253 kb on - strand at 1393.364 kb on + strand at 1393.364 kb on + strand at 1393.364 kb on + strand at 1393.387 kb on + strand at 1393.387 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.394 kb on - strand at 1393.489 kb on + strand at 1393.489 kb on + strand at 1393.490 kb on - strand at 1393.490 kb on - strand at 1393.490 kb on - strand at 1393.490 kb on - strand at 1393.500 kb on + strand at 1393.586 kb on + strand, within Pf1N1B4_1227 at 1393.586 kb on + strand, within Pf1N1B4_1227 at 1393.586 kb on + strand, within Pf1N1B4_1227 at 1393.587 kb on - strand, within Pf1N1B4_1227 at 1393.619 kb on - strand, within Pf1N1B4_1227 at 1393.646 kb on + strand, within Pf1N1B4_1227 at 1393.646 kb on + strand at 1393.646 kb on + strand, within Pf1N1B4_1227 at 1393.646 kb on + strand, within Pf1N1B4_1227 at 1393.647 kb on - strand at 1393.655 kb on + strand, within Pf1N1B4_1227 at 1393.656 kb on - strand, within Pf1N1B4_1227 at 1393.670 kb on + strand, within Pf1N1B4_1227 at 1393.692 kb on - strand, within Pf1N1B4_1227 at 1393.805 kb on + strand at 1393.806 kb on - strand at 1393.873 kb on + strand at 1393.873 kb on + strand at 1393.873 kb on + strand at 1393.874 kb on - strand at 1393.874 kb on - strand at 1393.958 kb on - strand at 1393.958 kb on - strand at 1393.978 kb on - strand at 1393.978 kb on - strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.145 kb on - strand at 1394.145 kb on - strand at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1395.051 kb on + strand at 1395.051 kb on + strand at 1395.193 kb on + strand, within Pf1N1B4_1229 at 1395.194 kb on - strand at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.328 kb on - strand, within Pf1N1B4_1229 at 1395.328 kb on - strand, within Pf1N1B4_1229 at 1395.610 kb on + strand, within Pf1N1B4_1229 at 1395.736 kb on + strand at 1395.760 kb on - strand, within Pf1N1B4_1229 at 1395.760 kb on - strand, within Pf1N1B4_1229
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,393,088 + +0.0 1,393,144 + -0.4 1,393,144 + -0.4 1,393,181 + +0.6 1,393,182 - +0.8 1,393,188 + -0.8 1,393,189 - +0.8 1,393,214 - +2.0 1,393,225 + +2.5 1,393,227 + -0.4 1,393,227 + +3.2 1,393,227 + +0.2 1,393,228 - +1.4 1,393,228 - +1.2 1,393,230 + +1.0 1,393,253 - +0.8 1,393,364 + +0.4 1,393,364 + +0.4 1,393,364 + +0.6 1,393,387 + +0.3 1,393,387 + +1.2 1,393,393 + -0.4 1,393,393 + -0.2 1,393,393 + -0.1 1,393,393 + -0.3 1,393,393 + -0.6 1,393,393 + +0.2 1,393,393 + +0.4 1,393,393 + +0.7 1,393,394 - +1.8 1,393,489 + +1.8 1,393,489 + -0.0 1,393,490 - +0.2 1,393,490 - -0.4 1,393,490 - -0.8 1,393,490 - +0.5 1,393,500 + +0.4 1,393,586 + Pf1N1B4_1227 0.31 +0.4 1,393,586 + Pf1N1B4_1227 0.31 +0.2 1,393,586 + Pf1N1B4_1227 0.31 +0.5 1,393,587 - Pf1N1B4_1227 0.32 +0.2 1,393,619 - Pf1N1B4_1227 0.41 +0.7 1,393,646 + Pf1N1B4_1227 0.49 -1.1 1,393,646 + +2.2 1,393,646 + Pf1N1B4_1227 0.49 +1.4 1,393,646 + Pf1N1B4_1227 0.49 -1.7 1,393,647 - -0.4 1,393,655 + Pf1N1B4_1227 0.51 +0.5 1,393,656 - Pf1N1B4_1227 0.52 -0.1 1,393,670 + Pf1N1B4_1227 0.56 -0.8 1,393,692 - Pf1N1B4_1227 0.62 +0.9 1,393,805 + +2.2 1,393,806 - +0.5 1,393,873 + +0.2 1,393,873 + -0.4 1,393,873 + +0.5 1,393,874 - -1.0 1,393,874 - -0.8 1,393,958 - +0.6 1,393,958 - +0.6 1,393,978 - +1.2 1,393,978 - +0.4 1,394,144 + -1.0 1,394,144 + -0.4 1,394,144 + -0.3 1,394,144 + -1.8 1,394,145 - +2.8 1,394,145 - +0.8 1,394,303 + Pf1N1B4_1228 0.26 -1.5 1,394,303 + Pf1N1B4_1228 0.26 -0.6 1,394,303 + Pf1N1B4_1228 0.26 -1.2 1,394,303 + +1.6 1,394,303 + Pf1N1B4_1228 0.26 -0.4 1,394,304 - Pf1N1B4_1228 0.27 +1.2 1,394,304 - Pf1N1B4_1228 0.27 +0.4 1,394,304 - Pf1N1B4_1228 0.27 +0.4 1,394,304 - Pf1N1B4_1228 0.27 +0.2 1,394,304 - Pf1N1B4_1228 0.27 -1.2 1,394,304 - Pf1N1B4_1228 0.27 -1.9 1,394,304 - +1.6 1,394,304 - Pf1N1B4_1228 0.27 -0.9 1,394,304 - Pf1N1B4_1228 0.27 -1.2 1,395,051 + +0.4 1,395,051 + -0.8 1,395,193 + Pf1N1B4_1229 0.15 +0.9 1,395,194 - +0.2 1,395,327 + Pf1N1B4_1229 0.24 +0.3 1,395,327 + Pf1N1B4_1229 0.24 -0.4 1,395,327 + Pf1N1B4_1229 0.24 +1.4 1,395,327 + Pf1N1B4_1229 0.24 +0.5 1,395,327 + Pf1N1B4_1229 0.24 +1.2 1,395,327 + Pf1N1B4_1229 0.24 -0.8 1,395,327 + Pf1N1B4_1229 0.24 +0.2 1,395,328 - Pf1N1B4_1229 0.24 +0.8 1,395,328 - Pf1N1B4_1229 0.24 -1.9 1,395,610 + Pf1N1B4_1229 0.42 +0.3 1,395,736 + -0.4 1,395,760 - Pf1N1B4_1229 0.52 -2.5 1,395,760 - Pf1N1B4_1229 0.52 -0.4
Or see this region's nucleotide sequence