Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1212 and Pf1N1B4_1213 are separated by 84 nucleotides Pf1N1B4_1213 and Pf1N1B4_1214 are separated by 138 nucleotides Pf1N1B4_1214 and Pf1N1B4_1215 are separated by 80 nucleotides Pf1N1B4_1215 and Pf1N1B4_1216 are separated by 149 nucleotides
Pf1N1B4_1212 - Predicted flavin reductase RutF in novel pyrimidine catabolism pathway, at 1,375,077 to 1,375,379
_1212
Pf1N1B4_1213 - Transcriptional regulator, at 1,375,464 to 1,375,739
_1213
Pf1N1B4_1214 - Chorismate--pyruvate lyase (EC 4.1.3.40), at 1,375,878 to 1,376,408
_1214
Pf1N1B4_1215 - Transcriptional regulator, ArsR family, at 1,376,489 to 1,376,851
_1215
Pf1N1B4_1216 - Outer membrane component of tripartite multidrug resistance system, at 1,377,001 to 1,378,431
_1216
Position (kb)
1375
1376
1377 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1374.897 kb on - strand at 1375.320 kb on + strand, within Pf1N1B4_1212 at 1375.321 kb on - strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.469 kb on - strand at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.495 kb on + strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.514 kb on + strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.547 kb on + strand, within Pf1N1B4_1213 at 1375.717 kb on + strand at 1375.717 kb on + strand at 1375.718 kb on - strand at 1375.718 kb on - strand at 1375.817 kb on - strand at 1375.817 kb on - strand at 1375.817 kb on - strand at 1375.826 kb on + strand at 1375.994 kb on - strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.101 kb on + strand, within Pf1N1B4_1214 at 1376.293 kb on + strand, within Pf1N1B4_1214 at 1376.293 kb on + strand, within Pf1N1B4_1214 at 1376.293 kb on + strand, within Pf1N1B4_1214 at 1376.364 kb on + strand at 1376.365 kb on - strand at 1376.409 kb on - strand at 1376.409 kb on - strand at 1376.478 kb on - strand at 1376.478 kb on - strand at 1376.478 kb on - strand at 1376.478 kb on - strand at 1376.478 kb on - strand at 1376.641 kb on - strand, within Pf1N1B4_1215 at 1376.811 kb on - strand, within Pf1N1B4_1215 at 1376.814 kb on - strand, within Pf1N1B4_1215 at 1376.851 kb on + strand at 1376.851 kb on + strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.856 kb on - strand at 1376.856 kb on - strand at 1376.856 kb on - strand at 1376.856 kb on - strand at 1376.860 kb on - strand at 1376.860 kb on - strand at 1376.860 kb on - strand at 1376.872 kb on - strand at 1376.872 kb on - strand at 1376.872 kb on - strand at 1376.874 kb on - strand at 1376.874 kb on - strand at 1376.874 kb on - strand at 1376.890 kb on - strand at 1376.893 kb on - strand at 1376.893 kb on - strand at 1376.893 kb on - strand at 1376.904 kb on - strand at 1376.917 kb on - strand at 1376.917 kb on - strand at 1376.936 kb on - strand at 1376.936 kb on - strand at 1376.965 kb on - strand at 1376.965 kb on - strand at 1376.965 kb on - strand at 1376.965 kb on - strand at 1377.144 kb on + strand at 1377.144 kb on + strand, within Pf1N1B4_1216 at 1377.144 kb on + strand, within Pf1N1B4_1216 at 1377.144 kb on + strand, within Pf1N1B4_1216 at 1377.144 kb on + strand, within Pf1N1B4_1216 at 1377.145 kb on - strand, within Pf1N1B4_1216 at 1377.145 kb on - strand, within Pf1N1B4_1216 at 1377.145 kb on - strand, within Pf1N1B4_1216 at 1377.145 kb on - strand, within Pf1N1B4_1216 at 1377.145 kb on - strand, within Pf1N1B4_1216 at 1377.145 kb on - strand at 1377.145 kb on - strand, within Pf1N1B4_1216 at 1377.162 kb on - strand, within Pf1N1B4_1216 at 1377.162 kb on - strand, within Pf1N1B4_1216 at 1377.182 kb on + strand, within Pf1N1B4_1216 at 1377.182 kb on + strand, within Pf1N1B4_1216 at 1377.183 kb on - strand, within Pf1N1B4_1216 at 1377.183 kb on - strand, within Pf1N1B4_1216 at 1377.183 kb on - strand, within Pf1N1B4_1216 at 1377.289 kb on + strand, within Pf1N1B4_1216 at 1377.290 kb on - strand, within Pf1N1B4_1216 at 1377.290 kb on - strand, within Pf1N1B4_1216 at 1377.299 kb on + strand, within Pf1N1B4_1216 at 1377.300 kb on - strand, within Pf1N1B4_1216 at 1377.315 kb on + strand, within Pf1N1B4_1216 at 1377.316 kb on - strand, within Pf1N1B4_1216
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,374,897 - -0.0 1,375,320 + Pf1N1B4_1212 0.80 +1.2 1,375,321 - Pf1N1B4_1212 0.81 +0.4 1,375,335 + Pf1N1B4_1212 0.85 -0.1 1,375,335 + Pf1N1B4_1212 0.85 -1.5 1,375,335 + Pf1N1B4_1212 0.85 +1.8 1,375,335 + Pf1N1B4_1212 0.85 +0.4 1,375,335 + Pf1N1B4_1212 0.85 -0.4 1,375,335 + Pf1N1B4_1212 0.85 +0.6 1,375,336 - Pf1N1B4_1212 0.85 +0.2 1,375,336 - Pf1N1B4_1212 0.85 +1.6 1,375,469 - -0.7 1,375,494 - Pf1N1B4_1213 0.11 +0.0 1,375,494 - Pf1N1B4_1213 0.11 +0.8 1,375,495 + Pf1N1B4_1213 0.11 -1.1 1,375,496 - Pf1N1B4_1213 0.12 -0.4 1,375,496 - Pf1N1B4_1213 0.12 +0.6 1,375,502 + Pf1N1B4_1213 0.14 +0.4 1,375,502 + Pf1N1B4_1213 0.14 +1.2 1,375,503 - Pf1N1B4_1213 0.14 +0.2 1,375,503 - Pf1N1B4_1213 0.14 -1.1 1,375,503 - Pf1N1B4_1213 0.14 -1.0 1,375,503 - Pf1N1B4_1213 0.14 -0.1 1,375,503 - Pf1N1B4_1213 0.14 -1.5 1,375,514 + Pf1N1B4_1213 0.18 +0.3 1,375,515 - Pf1N1B4_1213 0.18 -0.1 1,375,515 - Pf1N1B4_1213 0.18 +1.5 1,375,515 - Pf1N1B4_1213 0.18 -0.4 1,375,515 - Pf1N1B4_1213 0.18 -0.4 1,375,547 + Pf1N1B4_1213 0.30 +1.8 1,375,717 + +0.8 1,375,717 + +0.2 1,375,718 - -0.7 1,375,718 - +0.9 1,375,817 - +0.2 1,375,817 - +0.5 1,375,817 - +0.5 1,375,826 + -0.2 1,375,994 - Pf1N1B4_1214 0.22 +0.2 1,376,069 + Pf1N1B4_1214 0.36 +0.9 1,376,069 + Pf1N1B4_1214 0.36 +0.2 1,376,069 + Pf1N1B4_1214 0.36 +0.4 1,376,069 + Pf1N1B4_1214 0.36 +0.6 1,376,069 + Pf1N1B4_1214 0.36 +0.5 1,376,069 + Pf1N1B4_1214 0.36 -0.7 1,376,069 + Pf1N1B4_1214 0.36 -0.8 1,376,069 + Pf1N1B4_1214 0.36 +0.7 1,376,070 - Pf1N1B4_1214 0.36 +0.9 1,376,070 - Pf1N1B4_1214 0.36 +0.2 1,376,070 - Pf1N1B4_1214 0.36 +1.2 1,376,070 - Pf1N1B4_1214 0.36 +0.8 1,376,070 - Pf1N1B4_1214 0.36 +2.3 1,376,070 - Pf1N1B4_1214 0.36 +0.8 1,376,070 - Pf1N1B4_1214 0.36 -0.1 1,376,070 - Pf1N1B4_1214 0.36 +1.0 1,376,101 + Pf1N1B4_1214 0.42 +1.6 1,376,293 + Pf1N1B4_1214 0.78 +0.2 1,376,293 + Pf1N1B4_1214 0.78 -0.0 1,376,293 + Pf1N1B4_1214 0.78 -1.4 1,376,364 + -0.3 1,376,365 - +1.2 1,376,409 - -1.0 1,376,409 - -0.7 1,376,478 - +0.5 1,376,478 - -1.0 1,376,478 - -1.4 1,376,478 - -1.4 1,376,478 - +0.8 1,376,641 - Pf1N1B4_1215 0.42 -1.1 1,376,811 - Pf1N1B4_1215 0.89 +1.2 1,376,814 - Pf1N1B4_1215 0.90 -2.1 1,376,851 + -0.1 1,376,851 + +0.6 1,376,852 - -1.8 1,376,852 - -1.8 1,376,852 - +1.2 1,376,852 - +1.2 1,376,852 - -0.4 1,376,852 - +0.6 1,376,852 - -0.8 1,376,852 - -1.4 1,376,856 - +1.2 1,376,856 - -1.4 1,376,856 - +0.2 1,376,856 - -1.1 1,376,860 - -2.0 1,376,860 - -2.2 1,376,860 - -0.5 1,376,872 - +0.2 1,376,872 - -0.6 1,376,872 - +0.2 1,376,874 - -0.8 1,376,874 - -1.0 1,376,874 - +0.5 1,376,890 - -0.0 1,376,893 - -0.0 1,376,893 - -0.7 1,376,893 - +1.2 1,376,904 - +0.6 1,376,917 - -0.7 1,376,917 - +0.0 1,376,936 - +0.5 1,376,936 - +1.2 1,376,965 - -1.6 1,376,965 - -0.7 1,376,965 - -1.0 1,376,965 - -0.2 1,377,144 + +1.6 1,377,144 + Pf1N1B4_1216 0.10 +0.7 1,377,144 + Pf1N1B4_1216 0.10 -0.4 1,377,144 + Pf1N1B4_1216 0.10 +0.5 1,377,144 + Pf1N1B4_1216 0.10 +2.2 1,377,145 - Pf1N1B4_1216 0.10 +0.3 1,377,145 - Pf1N1B4_1216 0.10 -0.6 1,377,145 - Pf1N1B4_1216 0.10 -0.3 1,377,145 - Pf1N1B4_1216 0.10 -0.2 1,377,145 - Pf1N1B4_1216 0.10 -0.0 1,377,145 - +1.2 1,377,145 - Pf1N1B4_1216 0.10 -2.1 1,377,162 - Pf1N1B4_1216 0.11 +0.0 1,377,162 - Pf1N1B4_1216 0.11 +0.2 1,377,182 + Pf1N1B4_1216 0.13 +0.2 1,377,182 + Pf1N1B4_1216 0.13 +0.2 1,377,183 - Pf1N1B4_1216 0.13 +0.2 1,377,183 - Pf1N1B4_1216 0.13 -2.1 1,377,183 - Pf1N1B4_1216 0.13 -0.2 1,377,289 + Pf1N1B4_1216 0.20 +1.2 1,377,290 - Pf1N1B4_1216 0.20 -0.2 1,377,290 - Pf1N1B4_1216 0.20 +1.0 1,377,299 + Pf1N1B4_1216 0.21 +0.8 1,377,300 - Pf1N1B4_1216 0.21 -0.7 1,377,315 + Pf1N1B4_1216 0.22 +0.2 1,377,316 - Pf1N1B4_1216 0.22 +0.0
Or see this region's nucleotide sequence