Experiment: PYE with Cisplatin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03706 and CCNA_03707 are separated by 85 nucleotides CCNA_03707 and CCNA_03708 are separated by 13 nucleotides CCNA_03708 and CCNA_03709 are separated by 14 nucleotides CCNA_03709 and CCNA_03710 are separated by 164 nucleotides CCNA_03710 and CCNA_03711 are separated by 7 nucleotides
CCNA_03706: CCNA_03706 - small heat shock protein, at 3,874,686 to 3,875,114
_03706
CCNA_03707: CCNA_03707 - hypothetical protein, at 3,875,200 to 3,875,469
_03707
CCNA_03708: CCNA_03708 - transposase, at 3,875,483 to 3,875,851
_03708
CCNA_03709: CCNA_03709 - transposase, at 3,875,866 to 3,876,276
_03709
CCNA_03710: CCNA_03710 - nitrogen regulatory IIA protein, at 3,876,441 to 3,876,983
_03710
CCNA_03711: CCNA_03711 - ribosome-associated factor Y, at 3,876,991 to 3,877,617
_03711
Position (kb)
3875
3876
3877 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3874.870 kb on - strand, within CCNA_03706 at 3874.928 kb on + strand, within CCNA_03706 at 3874.946 kb on - strand, within CCNA_03706 at 3874.955 kb on - strand, within CCNA_03706 at 3874.975 kb on + strand, within CCNA_03706 at 3874.975 kb on + strand, within CCNA_03706 at 3874.976 kb on - strand, within CCNA_03706 at 3875.012 kb on + strand, within CCNA_03706 at 3875.013 kb on - strand, within CCNA_03706 at 3875.013 kb on - strand at 3875.034 kb on - strand, within CCNA_03706 at 3875.040 kb on - strand, within CCNA_03706 at 3875.060 kb on - strand, within CCNA_03706 at 3875.060 kb on - strand, within CCNA_03706 at 3875.060 kb on - strand, within CCNA_03706 at 3875.065 kb on - strand, within CCNA_03706 at 3875.119 kb on - strand at 3875.155 kb on - strand at 3875.160 kb on + strand at 3875.215 kb on + strand at 3875.215 kb on + strand at 3875.218 kb on - strand at 3875.218 kb on - strand at 3875.220 kb on - strand at 3875.220 kb on - strand at 3875.223 kb on - strand at 3875.227 kb on + strand, within CCNA_03707 at 3875.227 kb on + strand, within CCNA_03707 at 3875.227 kb on + strand, within CCNA_03707 at 3875.235 kb on - strand, within CCNA_03707 at 3875.235 kb on - strand, within CCNA_03707 at 3875.235 kb on - strand, within CCNA_03707 at 3875.235 kb on - strand, within CCNA_03707 at 3875.236 kb on + strand, within CCNA_03707 at 3875.244 kb on - strand, within CCNA_03707 at 3875.251 kb on + strand, within CCNA_03707 at 3875.265 kb on + strand, within CCNA_03707 at 3875.296 kb on + strand, within CCNA_03707 at 3875.301 kb on + strand, within CCNA_03707 at 3875.337 kb on + strand, within CCNA_03707 at 3875.370 kb on + strand, within CCNA_03707 at 3875.438 kb on + strand, within CCNA_03707 at 3875.438 kb on + strand, within CCNA_03707 at 3875.438 kb on + strand, within CCNA_03707 at 3875.438 kb on + strand, within CCNA_03707 at 3875.446 kb on - strand at 3875.463 kb on - strand at 3876.296 kb on + strand at 3876.297 kb on + strand at 3876.342 kb on - strand at 3876.436 kb on - strand at 3876.496 kb on + strand, within CCNA_03710 at 3876.583 kb on + strand, within CCNA_03710 at 3876.638 kb on + strand, within CCNA_03710 at 3876.638 kb on + strand, within CCNA_03710 at 3876.666 kb on + strand, within CCNA_03710 at 3876.666 kb on + strand, within CCNA_03710 at 3876.674 kb on - strand, within CCNA_03710 at 3876.686 kb on + strand, within CCNA_03710 at 3876.742 kb on - strand, within CCNA_03710 at 3876.769 kb on - strand, within CCNA_03710 at 3876.808 kb on - strand, within CCNA_03710 at 3876.825 kb on + strand, within CCNA_03710 at 3876.974 kb on + strand at 3876.975 kb on - strand at 3876.992 kb on - strand at 3876.994 kb on + strand at 3876.994 kb on + strand at 3876.997 kb on - strand at 3877.082 kb on - strand, within CCNA_03711 at 3877.195 kb on + strand, within CCNA_03711 at 3877.214 kb on + strand, within CCNA_03711 at 3877.214 kb on + strand, within CCNA_03711 at 3877.224 kb on - strand, within CCNA_03711 at 3877.228 kb on + strand, within CCNA_03711
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Cisplatin 0.005 mg/ml remove 3,874,870 - CCNA_03706 0.43 -1.9 3,874,928 + CCNA_03706 0.56 -1.2 3,874,946 - CCNA_03706 0.61 -3.0 3,874,955 - CCNA_03706 0.63 +0.1 3,874,975 + CCNA_03706 0.67 -0.6 3,874,975 + CCNA_03706 0.67 -1.2 3,874,976 - CCNA_03706 0.68 +1.0 3,875,012 + CCNA_03706 0.76 +1.2 3,875,013 - CCNA_03706 0.76 -2.8 3,875,013 - +0.6 3,875,034 - CCNA_03706 0.81 +0.0 3,875,040 - CCNA_03706 0.83 -1.1 3,875,060 - CCNA_03706 0.87 -1.5 3,875,060 - CCNA_03706 0.87 +0.3 3,875,060 - CCNA_03706 0.87 -1.6 3,875,065 - CCNA_03706 0.88 -2.1 3,875,119 - +0.7 3,875,155 - +1.0 3,875,160 + -0.0 3,875,215 + +0.3 3,875,215 + +0.7 3,875,218 - +1.3 3,875,218 - -0.4 3,875,220 - -0.6 3,875,220 - -0.6 3,875,223 - +1.5 3,875,227 + CCNA_03707 0.10 -0.6 3,875,227 + CCNA_03707 0.10 -0.8 3,875,227 + CCNA_03707 0.10 -1.2 3,875,235 - CCNA_03707 0.13 -1.5 3,875,235 - CCNA_03707 0.13 +0.9 3,875,235 - CCNA_03707 0.13 -0.2 3,875,235 - CCNA_03707 0.13 +0.4 3,875,236 + CCNA_03707 0.13 +0.8 3,875,244 - CCNA_03707 0.16 +1.0 3,875,251 + CCNA_03707 0.19 -0.0 3,875,265 + CCNA_03707 0.24 +0.6 3,875,296 + CCNA_03707 0.36 +0.3 3,875,301 + CCNA_03707 0.37 -0.4 3,875,337 + CCNA_03707 0.51 +0.3 3,875,370 + CCNA_03707 0.63 +0.6 3,875,438 + CCNA_03707 0.88 +0.6 3,875,438 + CCNA_03707 0.88 +0.5 3,875,438 + CCNA_03707 0.88 +0.5 3,875,438 + CCNA_03707 0.88 +0.2 3,875,446 - +0.7 3,875,463 - +0.8 3,876,296 + +0.8 3,876,297 + -0.1 3,876,342 - +1.5 3,876,436 - +0.3 3,876,496 + CCNA_03710 0.10 -1.8 3,876,583 + CCNA_03710 0.26 -2.5 3,876,638 + CCNA_03710 0.36 -2.7 3,876,638 + CCNA_03710 0.36 -3.6 3,876,666 + CCNA_03710 0.41 -1.5 3,876,666 + CCNA_03710 0.41 -0.5 3,876,674 - CCNA_03710 0.43 -2.8 3,876,686 + CCNA_03710 0.45 -1.7 3,876,742 - CCNA_03710 0.55 -3.1 3,876,769 - CCNA_03710 0.60 -1.6 3,876,808 - CCNA_03710 0.68 -2.3 3,876,825 + CCNA_03710 0.71 -0.8 3,876,974 + +0.5 3,876,975 - +1.9 3,876,992 - +1.3 3,876,994 + +0.6 3,876,994 + -2.5 3,876,997 - -0.2 3,877,082 - CCNA_03711 0.15 +0.6 3,877,195 + CCNA_03711 0.33 -0.4 3,877,214 + CCNA_03711 0.36 -1.2 3,877,214 + CCNA_03711 0.36 +0.5 3,877,224 - CCNA_03711 0.37 +0.2 3,877,228 + CCNA_03711 0.38 -0.8
Or see this region's nucleotide sequence