Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00189

Experiment: PYE with Cisplatin 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00188 and CCNA_00189 are separated by 143 nucleotidesCCNA_00189 and CCNA_00190 are separated by 1 nucleotidesCCNA_00190 and CCNA_00191 are separated by 125 nucleotides CCNA_00188: CCNA_00188 - ABC transporter ATP-binding protein, at 202,763 to 203,725 _00188 CCNA_00189: CCNA_00189 - zinc-finger domain protein, at 203,869 to 204,135 _00189 CCNA_00190: CCNA_00190 - acyl-CoA hydrolase, at 204,137 to 204,571 _00190 CCNA_00191: CCNA_00191 - N-acetylglutamate synthase domain protein, at 204,697 to 205,173 _00191 Position (kb) 203 204 205Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 202.899 kb on + strand, within CCNA_00188at 202.899 kb on + strand, within CCNA_00188at 202.899 kb on + strand, within CCNA_00188at 202.899 kb on + strand, within CCNA_00188at 202.904 kb on + strand, within CCNA_00188at 202.907 kb on - strand, within CCNA_00188at 202.912 kb on - strand, within CCNA_00188at 202.912 kb on - strand, within CCNA_00188at 202.922 kb on - strand, within CCNA_00188at 202.934 kb on + strand, within CCNA_00188at 202.967 kb on + strand, within CCNA_00188at 202.975 kb on - strand, within CCNA_00188at 202.975 kb on - strand, within CCNA_00188at 202.975 kb on - strand, within CCNA_00188at 202.994 kb on + strand, within CCNA_00188at 202.994 kb on + strand, within CCNA_00188at 203.002 kb on - strand, within CCNA_00188at 203.014 kb on + strand, within CCNA_00188at 203.033 kb on + strand, within CCNA_00188at 203.033 kb on + strand, within CCNA_00188at 203.068 kb on - strand, within CCNA_00188at 203.075 kb on - strand, within CCNA_00188at 203.107 kb on + strand, within CCNA_00188at 203.108 kb on - strand, within CCNA_00188at 203.113 kb on - strand, within CCNA_00188at 203.116 kb on + strand, within CCNA_00188at 203.116 kb on + strand, within CCNA_00188at 203.116 kb on + strand, within CCNA_00188at 203.124 kb on - strand, within CCNA_00188at 203.124 kb on - strand, within CCNA_00188at 203.132 kb on + strand, within CCNA_00188at 203.152 kb on - strand, within CCNA_00188at 203.158 kb on - strand, within CCNA_00188at 203.186 kb on - strand, within CCNA_00188at 203.187 kb on - strandat 203.197 kb on + strand, within CCNA_00188at 203.234 kb on - strand, within CCNA_00188at 203.246 kb on + strand, within CCNA_00188at 203.332 kb on + strand, within CCNA_00188at 203.332 kb on + strand, within CCNA_00188at 203.332 kb on + strand, within CCNA_00188at 203.335 kb on - strand, within CCNA_00188at 203.340 kb on - strand, within CCNA_00188at 203.340 kb on - strand, within CCNA_00188at 203.340 kb on - strand, within CCNA_00188at 203.340 kb on - strand, within CCNA_00188at 203.340 kb on - strand, within CCNA_00188at 203.370 kb on + strand, within CCNA_00188at 203.378 kb on - strand, within CCNA_00188at 203.378 kb on - strand, within CCNA_00188at 203.429 kb on - strand, within CCNA_00188at 203.434 kb on - strand, within CCNA_00188at 203.439 kb on - strand, within CCNA_00188at 203.452 kb on + strand, within CCNA_00188at 203.455 kb on - strand, within CCNA_00188at 203.510 kb on + strand, within CCNA_00188at 203.510 kb on + strand, within CCNA_00188at 203.510 kb on + strand, within CCNA_00188at 203.510 kb on + strand, within CCNA_00188at 203.510 kb on + strand, within CCNA_00188at 203.518 kb on - strand, within CCNA_00188at 203.518 kb on - strand, within CCNA_00188at 203.520 kb on + strand, within CCNA_00188at 203.520 kb on + strand, within CCNA_00188at 203.571 kb on - strand, within CCNA_00188at 203.590 kb on - strand, within CCNA_00188at 203.605 kb on - strand, within CCNA_00188at 203.626 kb on + strand, within CCNA_00188at 203.626 kb on + strand, within CCNA_00188at 203.627 kb on - strand, within CCNA_00188at 203.634 kb on - strandat 203.644 kb on - strandat 203.663 kb on - strandat 203.673 kb on + strandat 203.678 kb on + strandat 203.679 kb on + strandat 203.680 kb on - strandat 203.680 kb on - strandat 203.684 kb on + strandat 203.687 kb on - strandat 203.687 kb on - strandat 203.692 kb on - strandat 203.697 kb on - strandat 203.719 kb on + strandat 203.727 kb on - strandat 203.731 kb on + strandat 203.731 kb on + strandat 203.731 kb on + strandat 203.739 kb on - strandat 203.739 kb on - strandat 203.739 kb on - strandat 203.739 kb on - strandat 203.739 kb on - strandat 203.739 kb on - strandat 203.739 kb on - strandat 203.739 kb on - strandat 203.772 kb on + strandat 203.773 kb on - strandat 203.810 kb on + strandat 203.818 kb on - strandat 203.818 kb on - strandat 203.885 kb on + strandat 203.986 kb on + strand, within CCNA_00189at 204.008 kb on + strand, within CCNA_00189at 204.008 kb on + strand, within CCNA_00189at 204.008 kb on + strand, within CCNA_00189at 204.016 kb on - strand, within CCNA_00189at 204.102 kb on - strand, within CCNA_00189at 204.134 kb on + strandat 204.142 kb on - strandat 204.435 kb on - strand, within CCNA_00190at 204.461 kb on + strand, within CCNA_00190at 204.506 kb on - strand, within CCNA_00190at 204.561 kb on - strandat 204.583 kb on - strandat 204.703 kb on + strandat 204.777 kb on + strand, within CCNA_00191at 204.777 kb on + strand, within CCNA_00191at 204.777 kb on + strand, within CCNA_00191at 204.777 kb on + strand, within CCNA_00191at 204.792 kb on + strand, within CCNA_00191at 204.836 kb on + strand, within CCNA_00191at 204.836 kb on + strand, within CCNA_00191at 204.842 kb on - strand, within CCNA_00191at 204.881 kb on - strand, within CCNA_00191at 204.891 kb on - strand, within CCNA_00191at 204.901 kb on + strand, within CCNA_00191at 204.906 kb on + strand, within CCNA_00191at 204.906 kb on + strand, within CCNA_00191at 204.914 kb on - strand, within CCNA_00191at 204.974 kb on + strand, within CCNA_00191at 204.985 kb on + strand, within CCNA_00191at 204.988 kb on - strand, within CCNA_00191at 205.009 kb on - strand, within CCNA_00191at 205.041 kb on + strand, within CCNA_00191at 205.048 kb on + strand, within CCNA_00191at 205.077 kb on + strand, within CCNA_00191at 205.102 kb on + strand, within CCNA_00191at 205.105 kb on + strand, within CCNA_00191at 205.115 kb on - strand, within CCNA_00191at 205.120 kb on - strand, within CCNA_00191

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Cisplatin 0.005 mg/ml
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202,899 + CCNA_00188 0.14 +0.6
202,899 + CCNA_00188 0.14 -1.1
202,899 + CCNA_00188 0.14 -1.8
202,899 + CCNA_00188 0.14 +2.5
202,904 + CCNA_00188 0.15 -0.3
202,907 - CCNA_00188 0.15 +0.7
202,912 - CCNA_00188 0.15 +0.8
202,912 - CCNA_00188 0.15 -0.8
202,922 - CCNA_00188 0.17 +2.8
202,934 + CCNA_00188 0.18 -1.1
202,967 + CCNA_00188 0.21 -2.4
202,975 - CCNA_00188 0.22 +0.8
202,975 - CCNA_00188 0.22 -0.2
202,975 - CCNA_00188 0.22 +0.7
202,994 + CCNA_00188 0.24 -0.1
202,994 + CCNA_00188 0.24 +0.4
203,002 - CCNA_00188 0.25 +0.5
203,014 + CCNA_00188 0.26 -0.2
203,033 + CCNA_00188 0.28 +0.2
203,033 + CCNA_00188 0.28 +0.1
203,068 - CCNA_00188 0.32 +0.4
203,075 - CCNA_00188 0.32 +1.0
203,107 + CCNA_00188 0.36 +1.3
203,108 - CCNA_00188 0.36 +0.2
203,113 - CCNA_00188 0.36 -0.2
203,116 + CCNA_00188 0.37 +1.0
203,116 + CCNA_00188 0.37 -0.7
203,116 + CCNA_00188 0.37 -3.3
203,124 - CCNA_00188 0.37 +0.7
203,124 - CCNA_00188 0.37 -0.2
203,132 + CCNA_00188 0.38 -1.0
203,152 - CCNA_00188 0.40 +0.6
203,158 - CCNA_00188 0.41 +0.6
203,186 - CCNA_00188 0.44 +0.2
203,187 - +4.0
203,197 + CCNA_00188 0.45 -1.9
203,234 - CCNA_00188 0.49 +0.3
203,246 + CCNA_00188 0.50 -2.7
203,332 + CCNA_00188 0.59 -1.9
203,332 + CCNA_00188 0.59 -0.4
203,332 + CCNA_00188 0.59 -0.1
203,335 - CCNA_00188 0.59 +0.9
203,340 - CCNA_00188 0.60 +1.0
203,340 - CCNA_00188 0.60 +0.9
203,340 - CCNA_00188 0.60 +1.5
203,340 - CCNA_00188 0.60 +0.9
203,340 - CCNA_00188 0.60 -2.4
203,370 + CCNA_00188 0.63 -1.2
203,378 - CCNA_00188 0.64 +0.3
203,378 - CCNA_00188 0.64 +1.0
203,429 - CCNA_00188 0.69 -0.3
203,434 - CCNA_00188 0.70 +0.7
203,439 - CCNA_00188 0.70 -0.3
203,452 + CCNA_00188 0.72 +0.1
203,455 - CCNA_00188 0.72 +1.1
203,510 + CCNA_00188 0.78 -2.1
203,510 + CCNA_00188 0.78 -1.0
203,510 + CCNA_00188 0.78 +0.0
203,510 + CCNA_00188 0.78 +0.5
203,510 + CCNA_00188 0.78 -1.9
203,518 - CCNA_00188 0.78 +1.6
203,518 - CCNA_00188 0.78 +1.0
203,520 + CCNA_00188 0.79 -0.6
203,520 + CCNA_00188 0.79 -1.7
203,571 - CCNA_00188 0.84 +0.7
203,590 - CCNA_00188 0.86 +1.5
203,605 - CCNA_00188 0.87 +0.3
203,626 + CCNA_00188 0.90 -0.2
203,626 + CCNA_00188 0.90 -1.3
203,627 - CCNA_00188 0.90 +1.1
203,634 - -0.5
203,644 - +0.4
203,663 - +0.4
203,673 + -0.5
203,678 + -0.5
203,679 + -0.3
203,680 - +0.7
203,680 - +1.3
203,684 + -0.4
203,687 - -0.1
203,687 - +0.9
203,692 - +0.9
203,697 - -0.1
203,719 + -2.7
203,727 - -0.5
203,731 + -0.5
203,731 + +0.6
203,731 + -1.4
203,739 - -0.2
203,739 - +0.5
203,739 - +3.1
203,739 - +0.6
203,739 - +0.9
203,739 - +0.6
203,739 - +0.3
203,739 - +0.8
203,772 + -2.3
203,773 - -1.0
203,810 + -0.7
203,818 - +0.2
203,818 - -2.2
203,885 + +0.6
203,986 + CCNA_00189 0.44 +1.5
204,008 + CCNA_00189 0.52 +0.8
204,008 + CCNA_00189 0.52 +2.0
204,008 + CCNA_00189 0.52 +2.3
204,016 - CCNA_00189 0.55 -0.2
204,102 - CCNA_00189 0.87 -2.9
204,134 + -0.8
204,142 - -1.2
204,435 - CCNA_00190 0.69 -0.9
204,461 + CCNA_00190 0.74 -1.1
204,506 - CCNA_00190 0.85 -1.0
204,561 - -1.1
204,583 - +0.7
204,703 + +0.8
204,777 + CCNA_00191 0.17 -1.9
204,777 + CCNA_00191 0.17 +1.0
204,777 + CCNA_00191 0.17 -1.0
204,777 + CCNA_00191 0.17 -0.5
204,792 + CCNA_00191 0.20 -1.1
204,836 + CCNA_00191 0.29 -1.8
204,836 + CCNA_00191 0.29 -0.7
204,842 - CCNA_00191 0.30 -0.4
204,881 - CCNA_00191 0.39 -1.0
204,891 - CCNA_00191 0.41 +0.5
204,901 + CCNA_00191 0.43 -0.4
204,906 + CCNA_00191 0.44 +0.9
204,906 + CCNA_00191 0.44 -0.4
204,914 - CCNA_00191 0.45 -0.5
204,974 + CCNA_00191 0.58 +3.2
204,985 + CCNA_00191 0.60 -1.5
204,988 - CCNA_00191 0.61 -0.1
205,009 - CCNA_00191 0.65 -1.0
205,041 + CCNA_00191 0.72 -0.5
205,048 + CCNA_00191 0.74 -0.7
205,077 + CCNA_00191 0.80 -0.9
205,102 + CCNA_00191 0.85 -1.9
205,105 + CCNA_00191 0.86 -2.5
205,115 - CCNA_00191 0.88 +1.5
205,120 - CCNA_00191 0.89 -0.3

Or see this region's nucleotide sequence