Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_691 and Pf1N1B4_692 are separated by 15 nucleotides Pf1N1B4_692 and Pf1N1B4_693 overlap by 4 nucleotides Pf1N1B4_693 and Pf1N1B4_694 are separated by 293 nucleotides
Pf1N1B4_691 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 789,195 to 790,250
_691
Pf1N1B4_692 - ABC spermidine/putrescine transporter, inner membrane subunit, at 790,266 to 791,114
_692
Pf1N1B4_693 - ABC spermidine/putrescine transporter, inner membrane subunit, at 791,111 to 791,917
_693
Pf1N1B4_694 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 792,211 to 793,314
_694
Position (kb)
791
792 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.391 kb on - strand, within Pf1N1B4_692 at 790.391 kb on - strand, within Pf1N1B4_692 at 790.391 kb on - strand, within Pf1N1B4_692 at 790.391 kb on - strand, within Pf1N1B4_692 at 790.427 kb on - strand, within Pf1N1B4_692 at 790.427 kb on - strand, within Pf1N1B4_692 at 790.435 kb on + strand, within Pf1N1B4_692 at 790.436 kb on - strand, within Pf1N1B4_692 at 790.436 kb on - strand, within Pf1N1B4_692 at 790.798 kb on + strand, within Pf1N1B4_692 at 790.798 kb on + strand, within Pf1N1B4_692 at 790.799 kb on - strand at 790.799 kb on - strand, within Pf1N1B4_692 at 790.799 kb on - strand, within Pf1N1B4_692 at 790.799 kb on - strand, within Pf1N1B4_692 at 790.933 kb on + strand at 790.934 kb on - strand, within Pf1N1B4_692 at 790.948 kb on + strand, within Pf1N1B4_692 at 790.948 kb on + strand, within Pf1N1B4_692 at 790.948 kb on + strand, within Pf1N1B4_692 at 790.949 kb on - strand, within Pf1N1B4_692 at 790.949 kb on - strand, within Pf1N1B4_692 at 790.949 kb on - strand, within Pf1N1B4_692 at 791.154 kb on + strand at 791.154 kb on + strand at 791.155 kb on - strand at 791.155 kb on - strand at 791.155 kb on - strand at 791.433 kb on + strand, within Pf1N1B4_693 at 791.530 kb on - strand, within Pf1N1B4_693 at 791.530 kb on - strand, within Pf1N1B4_693 at 791.530 kb on - strand, within Pf1N1B4_693 at 791.530 kb on - strand, within Pf1N1B4_693 at 791.712 kb on + strand, within Pf1N1B4_693 at 791.712 kb on + strand, within Pf1N1B4_693 at 791.713 kb on - strand, within Pf1N1B4_693 at 791.793 kb on + strand at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand, within Pf1N1B4_693 at 791.797 kb on - strand at 791.817 kb on + strand, within Pf1N1B4_693 at 791.877 kb on - strand at 792.113 kb on + strand at 792.113 kb on + strand at 792.187 kb on + strand at 792.187 kb on + strand at 792.188 kb on - strand at 792.188 kb on - strand at 792.323 kb on + strand, within Pf1N1B4_694 at 792.323 kb on + strand, within Pf1N1B4_694 at 792.323 kb on + strand, within Pf1N1B4_694 at 792.323 kb on + strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.324 kb on - strand, within Pf1N1B4_694 at 792.380 kb on + strand, within Pf1N1B4_694 at 792.380 kb on + strand, within Pf1N1B4_694 at 792.380 kb on + strand, within Pf1N1B4_694 at 792.380 kb on + strand, within Pf1N1B4_694 at 792.380 kb on + strand at 792.380 kb on + strand, within Pf1N1B4_694 at 792.381 kb on - strand, within Pf1N1B4_694 at 792.381 kb on - strand, within Pf1N1B4_694 at 792.381 kb on - strand, within Pf1N1B4_694 at 792.441 kb on - strand, within Pf1N1B4_694 at 792.441 kb on - strand, within Pf1N1B4_694 at 792.500 kb on + strand, within Pf1N1B4_694 at 792.500 kb on + strand, within Pf1N1B4_694 at 792.500 kb on + strand, within Pf1N1B4_694 at 792.501 kb on - strand, within Pf1N1B4_694 at 792.539 kb on + strand, within Pf1N1B4_694 at 792.540 kb on - strand, within Pf1N1B4_694 at 792.581 kb on + strand, within Pf1N1B4_694 at 792.581 kb on + strand, within Pf1N1B4_694 at 792.582 kb on - strand, within Pf1N1B4_694 at 792.666 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.819 kb on - strand, within Pf1N1B4_694 at 792.819 kb on - strand, within Pf1N1B4_694 at 792.855 kb on - strand, within Pf1N1B4_694
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 790,390 + Pf1N1B4_692 0.15 -0.2 790,390 + Pf1N1B4_692 0.15 -0.7 790,390 + Pf1N1B4_692 0.15 -0.6 790,390 + Pf1N1B4_692 0.15 -0.7 790,390 + Pf1N1B4_692 0.15 +2.3 790,390 + Pf1N1B4_692 0.15 +1.0 790,390 + Pf1N1B4_692 0.15 -2.2 790,391 - Pf1N1B4_692 0.15 -0.4 790,391 - Pf1N1B4_692 0.15 -0.2 790,391 - Pf1N1B4_692 0.15 -0.6 790,391 - Pf1N1B4_692 0.15 +0.3 790,427 - Pf1N1B4_692 0.19 +0.1 790,427 - Pf1N1B4_692 0.19 -0.3 790,435 + Pf1N1B4_692 0.20 +1.3 790,436 - Pf1N1B4_692 0.20 -2.6 790,436 - Pf1N1B4_692 0.20 +1.0 790,798 + Pf1N1B4_692 0.63 -0.6 790,798 + Pf1N1B4_692 0.63 -0.3 790,799 - +2.0 790,799 - Pf1N1B4_692 0.63 +1.1 790,799 - Pf1N1B4_692 0.63 +1.0 790,799 - Pf1N1B4_692 0.63 -1.3 790,933 + -0.6 790,934 - Pf1N1B4_692 0.79 -0.3 790,948 + Pf1N1B4_692 0.80 +2.0 790,948 + Pf1N1B4_692 0.80 +0.2 790,948 + Pf1N1B4_692 0.80 +0.1 790,949 - Pf1N1B4_692 0.80 -2.2 790,949 - Pf1N1B4_692 0.80 -0.0 790,949 - Pf1N1B4_692 0.80 +2.0 791,154 + -1.7 791,154 + -0.7 791,155 - +0.4 791,155 - +4.0 791,155 - -0.3 791,433 + Pf1N1B4_693 0.40 -2.0 791,530 - Pf1N1B4_693 0.52 -0.0 791,530 - Pf1N1B4_693 0.52 -0.7 791,530 - Pf1N1B4_693 0.52 -1.3 791,530 - Pf1N1B4_693 0.52 +1.0 791,712 + Pf1N1B4_693 0.74 -2.2 791,712 + Pf1N1B4_693 0.74 -0.5 791,713 - Pf1N1B4_693 0.75 -1.6 791,793 + +1.0 791,797 - Pf1N1B4_693 0.85 -1.0 791,797 - Pf1N1B4_693 0.85 +0.8 791,797 - Pf1N1B4_693 0.85 -0.0 791,797 - Pf1N1B4_693 0.85 -1.3 791,797 - Pf1N1B4_693 0.85 -0.6 791,797 - -0.0 791,817 + Pf1N1B4_693 0.87 -1.6 791,877 - +1.0 792,113 + +0.4 792,113 + -2.3 792,187 + +0.1 792,187 + -0.1 792,188 - +0.7 792,188 - -0.4 792,323 + Pf1N1B4_694 0.10 +0.3 792,323 + Pf1N1B4_694 0.10 -1.3 792,323 + Pf1N1B4_694 0.10 -0.0 792,323 + Pf1N1B4_694 0.10 -0.2 792,324 - Pf1N1B4_694 0.10 +2.0 792,324 - Pf1N1B4_694 0.10 +0.6 792,324 - Pf1N1B4_694 0.10 +1.0 792,324 - Pf1N1B4_694 0.10 +1.7 792,324 - Pf1N1B4_694 0.10 -0.4 792,380 + Pf1N1B4_694 0.15 -1.8 792,380 + Pf1N1B4_694 0.15 -0.3 792,380 + Pf1N1B4_694 0.15 +1.0 792,380 + Pf1N1B4_694 0.15 -1.0 792,380 + -1.0 792,380 + Pf1N1B4_694 0.15 -0.8 792,381 - Pf1N1B4_694 0.15 -0.0 792,381 - Pf1N1B4_694 0.15 +1.4 792,381 - Pf1N1B4_694 0.15 -0.4 792,441 - Pf1N1B4_694 0.21 -2.2 792,441 - Pf1N1B4_694 0.21 +0.5 792,500 + Pf1N1B4_694 0.26 +2.4 792,500 + Pf1N1B4_694 0.26 -0.7 792,500 + Pf1N1B4_694 0.26 -0.1 792,501 - Pf1N1B4_694 0.26 -0.3 792,539 + Pf1N1B4_694 0.30 -0.0 792,540 - Pf1N1B4_694 0.30 -0.8 792,581 + Pf1N1B4_694 0.34 +2.0 792,581 + Pf1N1B4_694 0.34 +1.0 792,582 - Pf1N1B4_694 0.34 -1.3 792,666 + Pf1N1B4_694 0.41 -0.6 792,818 + Pf1N1B4_694 0.55 -2.6 792,818 + Pf1N1B4_694 0.55 +1.0 792,818 + Pf1N1B4_694 0.55 -1.8 792,818 + Pf1N1B4_694 0.55 +1.4 792,819 - Pf1N1B4_694 0.55 -0.9 792,819 - Pf1N1B4_694 0.55 +0.8 792,855 - Pf1N1B4_694 0.58 +1.0
Or see this region's nucleotide sequence