Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_679 and Pf1N1B4_680 are separated by 134 nucleotides Pf1N1B4_680 and Pf1N1B4_681 overlap by 8 nucleotides
Pf1N1B4_679 - Enoyl-CoA hydratase (EC 4.2.1.17), at 776,267 to 777,016
_679
Pf1N1B4_680 - 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases, at 777,151 to 778,086
_680
Pf1N1B4_681 - FOG: GGDEF domain, at 778,079 to 779,185
_681
Position (kb) 777
778
779 Strain fitness (log2 ratio) -1
0
1 at 776.281 kb on + strand at 776.282 kb on - strand at 776.282 kb on - strand at 776.665 kb on - strand, within Pf1N1B4_679 at 776.773 kb on - strand, within Pf1N1B4_679 at 776.922 kb on + strand, within Pf1N1B4_679 at 776.922 kb on + strand, within Pf1N1B4_679 at 776.922 kb on + strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 777.163 kb on + strand at 777.209 kb on + strand at 777.210 kb on - strand at 777.210 kb on - strand at 777.383 kb on + strand, within Pf1N1B4_680 at 777.383 kb on + strand, within Pf1N1B4_680 at 777.384 kb on - strand, within Pf1N1B4_680 at 777.534 kb on - strand, within Pf1N1B4_680 at 777.680 kb on + strand, within Pf1N1B4_680 at 777.761 kb on - strand, within Pf1N1B4_680 at 777.990 kb on + strand at 777.991 kb on - strand, within Pf1N1B4_680 at 777.991 kb on - strand, within Pf1N1B4_680 at 777.991 kb on - strand, within Pf1N1B4_680 at 777.991 kb on - strand, within Pf1N1B4_680 at 777.993 kb on - strand at 778.266 kb on - strand, within Pf1N1B4_681 at 778.651 kb on + strand, within Pf1N1B4_681 at 778.712 kb on + strand, within Pf1N1B4_681 at 778.712 kb on + strand at 778.713 kb on - strand, within Pf1N1B4_681 at 778.713 kb on - strand, within Pf1N1B4_681 at 778.873 kb on - strand, within Pf1N1B4_681 at 778.873 kb on - strand, within Pf1N1B4_681 at 778.947 kb on - strand, within Pf1N1B4_681 at 778.947 kb on - strand, within Pf1N1B4_681 at 779.076 kb on + strand at 779.077 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 776,281 + +1.0 776,282 - +1.0 776,282 - -0.5 776,665 - Pf1N1B4_679 0.53 +0.2 776,773 - Pf1N1B4_679 0.67 +0.2 776,922 + Pf1N1B4_679 0.87 -0.1 776,922 + Pf1N1B4_679 0.87 -1.3 776,922 + Pf1N1B4_679 0.87 -1.1 776,923 - Pf1N1B4_679 0.87 -0.3 776,923 - Pf1N1B4_679 0.87 -1.0 776,923 - Pf1N1B4_679 0.87 +0.7 776,923 - Pf1N1B4_679 0.87 +1.0 777,163 + +0.7 777,209 + +1.0 777,210 - -0.6 777,210 - -1.6 777,383 + Pf1N1B4_680 0.25 -1.5 777,383 + Pf1N1B4_680 0.25 -1.2 777,384 - Pf1N1B4_680 0.25 +0.2 777,534 - Pf1N1B4_680 0.41 -1.0 777,680 + Pf1N1B4_680 0.57 -0.3 777,761 - Pf1N1B4_680 0.65 -0.4 777,990 + +1.0 777,991 - Pf1N1B4_680 0.90 -1.5 777,991 - Pf1N1B4_680 0.90 -0.7 777,991 - Pf1N1B4_680 0.90 -1.3 777,991 - Pf1N1B4_680 0.90 +1.0 777,993 - -0.0 778,266 - Pf1N1B4_681 0.17 -0.0 778,651 + Pf1N1B4_681 0.52 +0.6 778,712 + Pf1N1B4_681 0.57 -1.3 778,712 + -0.3 778,713 - Pf1N1B4_681 0.57 -0.0 778,713 - Pf1N1B4_681 0.57 -0.6 778,873 - Pf1N1B4_681 0.72 +1.6 778,873 - Pf1N1B4_681 0.72 -0.5 778,947 - Pf1N1B4_681 0.78 +0.4 778,947 - Pf1N1B4_681 0.78 -1.2 779,076 + -1.5 779,077 - +0.4
Or see this region's nucleotide sequence