Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5973

Experiment: Choline Lysine 6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5971 and Pf1N1B4_5972 overlap by 8 nucleotidesPf1N1B4_5972 and Pf1N1B4_5973 are separated by 10 nucleotidesPf1N1B4_5973 and Pf1N1B4_5974 overlap by 8 nucleotidesPf1N1B4_5974 and Pf1N1B4_5975 are separated by 134 nucleotides Pf1N1B4_5971 - 2-Methylcitrate dehydratase AcnD, at 418,726 to 420,504 _5971 Pf1N1B4_5972 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 420,497 to 421,162 _5972 Pf1N1B4_5973 - Probable permease of ABC transporter, at 421,173 to 421,823 _5973 Pf1N1B4_5974 - ABC-type polar amino acid transport system, ATPase component, at 421,816 to 422,538 _5974 Pf1N1B4_5975 - 2-methylaconitate racemase, at 422,673 to 423,701 _5975 Position (kb) 421 422Strain fitness (log2 ratio) -2 -1 0 1 2at 420.192 kb on + strandat 420.352 kb on + strandat 420.352 kb on + strandat 420.352 kb on + strandat 420.353 kb on - strandat 420.353 kb on - strandat 420.353 kb on - strandat 420.353 kb on - strandat 420.507 kb on - strandat 420.507 kb on - strandat 420.720 kb on - strand, within Pf1N1B4_5972at 420.720 kb on - strand, within Pf1N1B4_5972at 420.729 kb on - strand, within Pf1N1B4_5972at 421.040 kb on + strand, within Pf1N1B4_5972at 421.041 kb on - strand, within Pf1N1B4_5972at 421.094 kb on + strand, within Pf1N1B4_5972at 421.095 kb on - strand, within Pf1N1B4_5972at 421.095 kb on - strand, within Pf1N1B4_5972at 421.098 kb on - strandat 421.458 kb on + strand, within Pf1N1B4_5973at 421.470 kb on + strand, within Pf1N1B4_5973at 421.748 kb on + strand, within Pf1N1B4_5973at 421.748 kb on + strand, within Pf1N1B4_5973at 421.749 kb on - strand, within Pf1N1B4_5973at 421.753 kb on - strand, within Pf1N1B4_5973at 421.755 kb on + strand, within Pf1N1B4_5973at 421.755 kb on + strand, within Pf1N1B4_5973at 421.835 kb on + strandat 421.835 kb on + strandat 421.835 kb on + strandat 421.835 kb on + strandat 421.835 kb on + strandat 421.835 kb on + strandat 421.836 kb on - strandat 421.836 kb on - strandat 421.836 kb on - strandat 421.839 kb on + strandat 421.839 kb on + strandat 421.839 kb on + strandat 421.840 kb on - strandat 421.840 kb on - strandat 421.840 kb on - strandat 421.840 kb on - strandat 422.284 kb on + strandat 422.285 kb on - strand, within Pf1N1B4_5974at 422.613 kb on + strandat 422.613 kb on + strandat 422.613 kb on + strandat 422.614 kb on - strandat 422.614 kb on - strandat 422.698 kb on + strandat 422.698 kb on + strandat 422.698 kb on + strandat 422.699 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 6 mM
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420,192 + +0.6
420,352 + +1.6
420,352 + +0.6
420,352 + +0.6
420,353 - -0.1
420,353 - +0.6
420,353 - -0.4
420,353 - -0.4
420,507 - -1.8
420,507 - +0.6
420,720 - Pf1N1B4_5972 0.33 +1.9
420,720 - Pf1N1B4_5972 0.33 +0.1
420,729 - Pf1N1B4_5972 0.35 +0.1
421,040 + Pf1N1B4_5972 0.82 -2.4
421,041 - Pf1N1B4_5972 0.82 +0.6
421,094 + Pf1N1B4_5972 0.90 +0.3
421,095 - Pf1N1B4_5972 0.90 +1.4
421,095 - Pf1N1B4_5972 0.90 +1.8
421,098 - -0.2
421,458 + Pf1N1B4_5973 0.44 -1.8
421,470 + Pf1N1B4_5973 0.46 +0.5
421,748 + Pf1N1B4_5973 0.88 -1.0
421,748 + Pf1N1B4_5973 0.88 -0.2
421,749 - Pf1N1B4_5973 0.88 -0.1
421,753 - Pf1N1B4_5973 0.89 -1.2
421,755 + Pf1N1B4_5973 0.89 -2.0
421,755 + Pf1N1B4_5973 0.89 -2.5
421,835 + -1.2
421,835 + +0.6
421,835 + +0.0
421,835 + +0.1
421,835 + +0.1
421,835 + +0.6
421,836 - -1.0
421,836 - +0.6
421,836 - +0.2
421,839 + -0.2
421,839 + +0.6
421,839 + -0.4
421,840 - +0.6
421,840 - +0.9
421,840 - -0.0
421,840 - +0.1
422,284 + -0.4
422,285 - Pf1N1B4_5974 0.65 -1.4
422,613 + -1.8
422,613 + +0.8
422,613 + +0.4
422,614 - -0.7
422,614 - -1.2
422,698 + -0.2
422,698 + +1.1
422,698 + -0.3
422,699 - -1.0

Or see this region's nucleotide sequence