Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5832 and Pf1N1B4_5833 are separated by 243 nucleotides Pf1N1B4_5833 and Pf1N1B4_5834 are separated by 36 nucleotides Pf1N1B4_5834 and Pf1N1B4_5835 are separated by 11 nucleotides
Pf1N1B4_5832 - LysR family transcriptional regulator near succinyl-CoA:3-ketoacid-coenzyme A transferase, at 266,702 to 267,601
_5832
Pf1N1B4_5833 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5), at 267,845 to 268,543
_5833
Pf1N1B4_5834 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5), at 268,580 to 269,245
_5834
Pf1N1B4_5835 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 269,257 to 270,438
_5835
Position (kb)
268
269
270 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 267.831 kb on + strand at 267.832 kb on - strand at 267.832 kb on - strand at 267.832 kb on - strand at 267.832 kb on - strand at 267.844 kb on + strand at 267.845 kb on - strand at 267.845 kb on - strand at 267.870 kb on - strand at 268.083 kb on - strand, within Pf1N1B4_5833 at 268.275 kb on - strand, within Pf1N1B4_5833 at 268.478 kb on + strand at 268.478 kb on + strand at 268.478 kb on + strand at 268.478 kb on + strand at 268.479 kb on - strand at 268.479 kb on - strand at 268.572 kb on + strand at 268.640 kb on + strand at 268.640 kb on + strand at 268.640 kb on + strand at 268.641 kb on - strand at 268.682 kb on + strand, within Pf1N1B4_5834 at 268.682 kb on + strand, within Pf1N1B4_5834 at 268.683 kb on - strand, within Pf1N1B4_5834 at 268.683 kb on - strand, within Pf1N1B4_5834 at 268.683 kb on - strand, within Pf1N1B4_5834 at 269.423 kb on + strand, within Pf1N1B4_5835 at 269.623 kb on + strand, within Pf1N1B4_5835 at 269.624 kb on - strand, within Pf1N1B4_5835 at 269.624 kb on - strand, within Pf1N1B4_5835 at 269.624 kb on - strand, within Pf1N1B4_5835 at 269.758 kb on + strand, within Pf1N1B4_5835 at 269.759 kb on - strand, within Pf1N1B4_5835 at 269.759 kb on - strand, within Pf1N1B4_5835
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 267,831 + +1.1 267,832 - +0.4 267,832 - +1.3 267,832 - +0.5 267,832 - +0.5 267,844 + -0.5 267,845 - -0.7 267,845 - +1.8 267,870 - +0.5 268,083 - Pf1N1B4_5833 0.34 -0.3 268,275 - Pf1N1B4_5833 0.62 -2.0 268,478 + -0.3 268,478 + +1.1 268,478 + -0.5 268,478 + +0.5 268,479 - -1.8 268,479 - -0.0 268,572 + +0.5 268,640 + +0.5 268,640 + -0.0 268,640 + +0.9 268,641 - -1.6 268,682 + Pf1N1B4_5834 0.15 -1.5 268,682 + Pf1N1B4_5834 0.15 +0.1 268,683 - Pf1N1B4_5834 0.15 -1.0 268,683 - Pf1N1B4_5834 0.15 -2.2 268,683 - Pf1N1B4_5834 0.15 +0.0 269,423 + Pf1N1B4_5835 0.14 -1.0 269,623 + Pf1N1B4_5835 0.31 +1.5 269,624 - Pf1N1B4_5835 0.31 -0.3 269,624 - Pf1N1B4_5835 0.31 +0.4 269,624 - Pf1N1B4_5835 0.31 -1.5 269,758 + Pf1N1B4_5835 0.42 +0.7 269,759 - Pf1N1B4_5835 0.42 -0.5 269,759 - Pf1N1B4_5835 0.42 -0.1
Or see this region's nucleotide sequence