Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5758 and Pf1N1B4_5759 are separated by 78 nucleotides Pf1N1B4_5759 and Pf1N1B4_5760 are separated by 3 nucleotides Pf1N1B4_5760 and Pf1N1B4_5761 are separated by 79 nucleotides Pf1N1B4_5761 and Pf1N1B4_5762 are separated by 11 nucleotides
Pf1N1B4_5758 - Nitric oxide reductase activation protein NorD, at 197,263 to 199,104
_5758
Pf1N1B4_5759 - Nitric-oxide reductase subunit B (EC 1.7.99.7), at 199,183 to 200,610
_5759
Pf1N1B4_5760 - Nitric-oxide reductase subunit C (EC 1.7.99.7), at 200,614 to 201,222
_5760
Pf1N1B4_5761 - Nitric oxide reductase activation protein NorF, at 201,302 to 201,559
_5761
Pf1N1B4_5762 - Nitric oxide reductase activation protein NorE, at 201,571 to 202,098
_5762
Position (kb)
199
200
201 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 198.369 kb on + strand at 198.837 kb on + strand, within Pf1N1B4_5758 at 199.167 kb on + strand at 199.193 kb on - strand at 199.243 kb on - strand at 199.251 kb on - strand at 199.515 kb on - strand, within Pf1N1B4_5759 at 199.553 kb on + strand at 199.554 kb on - strand at 199.554 kb on - strand at 199.669 kb on - strand, within Pf1N1B4_5759 at 199.887 kb on - strand, within Pf1N1B4_5759 at 199.887 kb on - strand, within Pf1N1B4_5759 at 199.887 kb on - strand, within Pf1N1B4_5759 at 200.010 kb on - strand, within Pf1N1B4_5759 at 200.043 kb on - strand, within Pf1N1B4_5759 at 200.144 kb on + strand, within Pf1N1B4_5759 at 200.382 kb on - strand at 200.489 kb on + strand at 200.615 kb on + strand at 200.616 kb on - strand at 200.640 kb on + strand at 200.641 kb on - strand at 200.641 kb on - strand at 200.770 kb on + strand, within Pf1N1B4_5760 at 200.770 kb on + strand, within Pf1N1B4_5760 at 200.770 kb on + strand, within Pf1N1B4_5760 at 201.015 kb on - strand at 201.015 kb on - strand, within Pf1N1B4_5760 at 201.015 kb on - strand, within Pf1N1B4_5760 at 201.015 kb on - strand, within Pf1N1B4_5760 at 201.018 kb on + strand, within Pf1N1B4_5760 at 201.087 kb on + strand, within Pf1N1B4_5760 at 201.330 kb on - strand, within Pf1N1B4_5761
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 198,369 + -0.5 198,837 + Pf1N1B4_5758 0.85 -0.1 199,167 + -0.5 199,193 - -2.1 199,243 - +0.5 199,251 - +0.5 199,515 - Pf1N1B4_5759 0.23 +0.9 199,553 + +1.5 199,554 - -0.5 199,554 - +1.1 199,669 - Pf1N1B4_5759 0.34 +0.2 199,887 - Pf1N1B4_5759 0.49 -1.5 199,887 - Pf1N1B4_5759 0.49 +2.7 199,887 - Pf1N1B4_5759 0.49 -0.5 200,010 - Pf1N1B4_5759 0.58 +1.5 200,043 - Pf1N1B4_5759 0.60 +2.1 200,144 + Pf1N1B4_5759 0.67 +0.7 200,382 - +2.8 200,489 + +0.8 200,615 + -0.2 200,616 - +1.1 200,640 + -0.2 200,641 - -0.6 200,641 - -0.6 200,770 + Pf1N1B4_5760 0.26 +1.5 200,770 + Pf1N1B4_5760 0.26 -0.0 200,770 + Pf1N1B4_5760 0.26 -0.7 201,015 - -0.5 201,015 - Pf1N1B4_5760 0.66 -1.1 201,015 - Pf1N1B4_5760 0.66 +0.5 201,015 - Pf1N1B4_5760 0.66 -0.8 201,018 + Pf1N1B4_5760 0.66 +0.9 201,087 + Pf1N1B4_5760 0.78 -1.3 201,330 - Pf1N1B4_5761 0.11 -0.7
Or see this region's nucleotide sequence