Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_573 and Pf1N1B4_574 are separated by 383 nucleotides Pf1N1B4_574 and Pf1N1B4_575 overlap by 4 nucleotides
Pf1N1B4_573 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 666,949 to 667,629
_573
Pf1N1B4_574 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 668,013 to 668,186
_574
Pf1N1B4_575 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 668,183 to 669,700
_575
Position (kb)
668
669 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 667.094 kb on + strand, within Pf1N1B4_573 at 667.094 kb on + strand, within Pf1N1B4_573 at 667.094 kb on + strand, within Pf1N1B4_573 at 667.094 kb on + strand, within Pf1N1B4_573 at 667.118 kb on + strand, within Pf1N1B4_573 at 667.118 kb on + strand at 667.118 kb on + strand, within Pf1N1B4_573 at 667.118 kb on + strand, within Pf1N1B4_573 at 667.118 kb on + strand, within Pf1N1B4_573 at 667.118 kb on + strand, within Pf1N1B4_573 at 667.118 kb on + strand, within Pf1N1B4_573 at 667.118 kb on + strand at 667.118 kb on + strand, within Pf1N1B4_573 at 667.118 kb on + strand, within Pf1N1B4_573 at 667.118 kb on + strand, within Pf1N1B4_573 at 667.118 kb on + strand, within Pf1N1B4_573 at 667.118 kb on + strand, within Pf1N1B4_573 at 667.119 kb on - strand, within Pf1N1B4_573 at 667.119 kb on - strand, within Pf1N1B4_573 at 667.119 kb on - strand, within Pf1N1B4_573 at 667.119 kb on - strand at 667.119 kb on - strand, within Pf1N1B4_573 at 667.119 kb on - strand, within Pf1N1B4_573 at 667.119 kb on - strand, within Pf1N1B4_573 at 667.119 kb on - strand, within Pf1N1B4_573 at 667.119 kb on - strand, within Pf1N1B4_573 at 667.119 kb on - strand, within Pf1N1B4_573 at 667.119 kb on - strand, within Pf1N1B4_573 at 667.119 kb on - strand, within Pf1N1B4_573 at 667.304 kb on + strand, within Pf1N1B4_573 at 667.304 kb on + strand, within Pf1N1B4_573 at 667.304 kb on + strand, within Pf1N1B4_573 at 667.304 kb on + strand, within Pf1N1B4_573 at 667.304 kb on + strand, within Pf1N1B4_573 at 667.305 kb on - strand, within Pf1N1B4_573 at 667.305 kb on - strand, within Pf1N1B4_573 at 667.305 kb on - strand at 667.317 kb on + strand, within Pf1N1B4_573 at 667.318 kb on - strand, within Pf1N1B4_573 at 667.422 kb on + strand, within Pf1N1B4_573 at 667.422 kb on + strand, within Pf1N1B4_573 at 667.422 kb on + strand, within Pf1N1B4_573 at 667.423 kb on - strand, within Pf1N1B4_573 at 667.436 kb on + strand, within Pf1N1B4_573 at 667.436 kb on + strand, within Pf1N1B4_573 at 667.436 kb on + strand, within Pf1N1B4_573 at 667.436 kb on + strand, within Pf1N1B4_573 at 667.436 kb on + strand, within Pf1N1B4_573 at 667.436 kb on + strand, within Pf1N1B4_573 at 667.436 kb on + strand, within Pf1N1B4_573 at 667.437 kb on - strand, within Pf1N1B4_573 at 667.437 kb on - strand, within Pf1N1B4_573 at 667.463 kb on + strand, within Pf1N1B4_573 at 667.463 kb on + strand, within Pf1N1B4_573 at 667.463 kb on + strand, within Pf1N1B4_573 at 667.464 kb on - strand, within Pf1N1B4_573 at 667.475 kb on + strand, within Pf1N1B4_573 at 667.475 kb on + strand, within Pf1N1B4_573 at 667.475 kb on + strand at 667.475 kb on + strand, within Pf1N1B4_573 at 667.475 kb on + strand, within Pf1N1B4_573 at 667.475 kb on + strand, within Pf1N1B4_573 at 667.475 kb on + strand, within Pf1N1B4_573 at 667.475 kb on + strand, within Pf1N1B4_573 at 667.476 kb on - strand, within Pf1N1B4_573 at 667.476 kb on - strand at 667.476 kb on - strand, within Pf1N1B4_573 at 667.476 kb on - strand, within Pf1N1B4_573 at 667.476 kb on - strand, within Pf1N1B4_573 at 667.476 kb on - strand, within Pf1N1B4_573 at 667.476 kb on - strand, within Pf1N1B4_573 at 667.476 kb on - strand, within Pf1N1B4_573 at 667.476 kb on - strand, within Pf1N1B4_573 at 667.476 kb on - strand, within Pf1N1B4_573 at 667.554 kb on + strand at 667.554 kb on + strand, within Pf1N1B4_573 at 667.555 kb on - strand, within Pf1N1B4_573 at 667.562 kb on + strand at 667.601 kb on + strand at 667.601 kb on + strand at 667.602 kb on - strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.605 kb on + strand at 667.606 kb on - strand at 667.606 kb on - strand at 667.606 kb on - strand at 667.606 kb on - strand at 667.606 kb on - strand at 667.606 kb on - strand at 667.606 kb on - strand at 667.606 kb on - strand at 667.606 kb on - strand at 667.608 kb on - strand at 667.662 kb on + strand at 667.662 kb on + strand at 667.662 kb on + strand at 667.680 kb on + strand at 667.680 kb on + strand at 667.680 kb on + strand at 667.680 kb on + strand at 667.680 kb on + strand at 667.680 kb on + strand at 667.680 kb on + strand at 667.680 kb on + strand at 667.680 kb on + strand at 667.680 kb on + strand at 667.680 kb on + strand at 667.681 kb on - strand at 667.681 kb on - strand at 667.683 kb on + strand at 667.683 kb on + strand at 667.683 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.715 kb on + strand at 667.716 kb on - strand at 667.720 kb on + strand at 667.720 kb on + strand at 667.720 kb on + strand at 667.720 kb on + strand at 667.720 kb on + strand at 667.720 kb on + strand at 667.720 kb on + strand at 667.720 kb on + strand at 667.751 kb on + strand at 667.751 kb on + strand at 667.751 kb on + strand at 667.751 kb on + strand at 667.751 kb on + strand at 667.751 kb on + strand at 667.751 kb on + strand at 667.751 kb on + strand at 667.751 kb on + strand at 667.774 kb on + strand at 667.793 kb on + strand at 667.793 kb on + strand at 667.795 kb on + strand at 667.795 kb on + strand at 667.795 kb on + strand at 667.795 kb on + strand at 667.796 kb on - strand at 667.796 kb on - strand at 667.825 kb on + strand at 667.826 kb on - strand at 667.849 kb on + strand at 667.849 kb on + strand at 667.849 kb on + strand at 667.891 kb on + strand at 667.891 kb on + strand at 667.891 kb on + strand at 667.957 kb on + strand at 667.957 kb on + strand at 667.957 kb on + strand at 667.958 kb on - strand at 668.105 kb on - strand, within Pf1N1B4_574 at 668.128 kb on + strand, within Pf1N1B4_574 at 668.128 kb on + strand, within Pf1N1B4_574 at 668.809 kb on + strand, within Pf1N1B4_575 at 668.809 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.890 kb on - strand, within Pf1N1B4_575 at 668.890 kb on - strand, within Pf1N1B4_575 at 668.890 kb on - strand, within Pf1N1B4_575 at 668.890 kb on - strand, within Pf1N1B4_575 at 668.890 kb on - strand, within Pf1N1B4_575 at 668.890 kb on - strand at 668.890 kb on - strand, within Pf1N1B4_575 at 668.893 kb on + strand, within Pf1N1B4_575 at 668.893 kb on + strand, within Pf1N1B4_575 at 668.893 kb on + strand, within Pf1N1B4_575 at 668.893 kb on + strand, within Pf1N1B4_575 at 668.893 kb on + strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.899 kb on - strand, within Pf1N1B4_575 at 668.899 kb on - strand, within Pf1N1B4_575 at 668.899 kb on - strand, within Pf1N1B4_575 at 669.060 kb on + strand, within Pf1N1B4_575 at 669.149 kb on - strand, within Pf1N1B4_575 at 669.149 kb on - strand, within Pf1N1B4_575 at 669.149 kb on - strand, within Pf1N1B4_575 at 669.166 kb on - strand, within Pf1N1B4_575 at 669.166 kb on - strand, within Pf1N1B4_575
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 667,094 + Pf1N1B4_573 0.21 -0.4 667,094 + Pf1N1B4_573 0.21 +1.4 667,094 + Pf1N1B4_573 0.21 +1.3 667,094 + Pf1N1B4_573 0.21 +0.9 667,118 + Pf1N1B4_573 0.25 -0.9 667,118 + +3.1 667,118 + Pf1N1B4_573 0.25 +0.6 667,118 + Pf1N1B4_573 0.25 +1.3 667,118 + Pf1N1B4_573 0.25 +0.5 667,118 + Pf1N1B4_573 0.25 -0.2 667,118 + Pf1N1B4_573 0.25 +2.2 667,118 + +0.9 667,118 + Pf1N1B4_573 0.25 +1.2 667,118 + Pf1N1B4_573 0.25 +1.7 667,118 + Pf1N1B4_573 0.25 -1.4 667,118 + Pf1N1B4_573 0.25 +0.3 667,118 + Pf1N1B4_573 0.25 +2.8 667,119 - Pf1N1B4_573 0.25 +0.3 667,119 - Pf1N1B4_573 0.25 +0.4 667,119 - Pf1N1B4_573 0.25 +0.3 667,119 - +0.9 667,119 - Pf1N1B4_573 0.25 -0.4 667,119 - Pf1N1B4_573 0.25 +1.5 667,119 - Pf1N1B4_573 0.25 -0.7 667,119 - Pf1N1B4_573 0.25 +0.9 667,119 - Pf1N1B4_573 0.25 +0.1 667,119 - Pf1N1B4_573 0.25 +0.7 667,119 - Pf1N1B4_573 0.25 +1.3 667,119 - Pf1N1B4_573 0.25 +0.9 667,304 + Pf1N1B4_573 0.52 +0.9 667,304 + Pf1N1B4_573 0.52 +2.3 667,304 + Pf1N1B4_573 0.52 +0.7 667,304 + Pf1N1B4_573 0.52 +0.5 667,304 + Pf1N1B4_573 0.52 +2.0 667,305 - Pf1N1B4_573 0.52 -1.4 667,305 - Pf1N1B4_573 0.52 +0.0 667,305 - +0.9 667,317 + Pf1N1B4_573 0.54 -1.2 667,318 - Pf1N1B4_573 0.54 +0.0 667,422 + Pf1N1B4_573 0.69 -0.5 667,422 + Pf1N1B4_573 0.69 +0.3 667,422 + Pf1N1B4_573 0.69 +1.3 667,423 - Pf1N1B4_573 0.70 +0.7 667,436 + Pf1N1B4_573 0.72 +0.5 667,436 + Pf1N1B4_573 0.72 +1.9 667,436 + Pf1N1B4_573 0.72 +0.3 667,436 + Pf1N1B4_573 0.72 +0.5 667,436 + Pf1N1B4_573 0.72 +2.3 667,436 + Pf1N1B4_573 0.72 -0.1 667,436 + Pf1N1B4_573 0.72 -0.1 667,437 - Pf1N1B4_573 0.72 +0.8 667,437 - Pf1N1B4_573 0.72 -0.1 667,463 + Pf1N1B4_573 0.75 +1.3 667,463 + Pf1N1B4_573 0.75 +2.0 667,463 + Pf1N1B4_573 0.75 +0.2 667,464 - Pf1N1B4_573 0.76 +0.0 667,475 + Pf1N1B4_573 0.77 -0.1 667,475 + Pf1N1B4_573 0.77 +0.4 667,475 + +0.9 667,475 + Pf1N1B4_573 0.77 +1.6 667,475 + Pf1N1B4_573 0.77 +0.2 667,475 + Pf1N1B4_573 0.77 +1.6 667,475 + Pf1N1B4_573 0.77 -0.7 667,475 + Pf1N1B4_573 0.77 +1.6 667,476 - Pf1N1B4_573 0.77 -0.7 667,476 - -0.1 667,476 - Pf1N1B4_573 0.77 +1.8 667,476 - Pf1N1B4_573 0.77 -0.9 667,476 - Pf1N1B4_573 0.77 +0.5 667,476 - Pf1N1B4_573 0.77 +0.7 667,476 - Pf1N1B4_573 0.77 +0.1 667,476 - Pf1N1B4_573 0.77 +1.3 667,476 - Pf1N1B4_573 0.77 +1.0 667,476 - Pf1N1B4_573 0.77 +2.9 667,554 + +2.5 667,554 + Pf1N1B4_573 0.89 +1.7 667,555 - Pf1N1B4_573 0.89 +2.3 667,562 + +2.1 667,601 + +0.6 667,601 + -0.1 667,602 - +0.1 667,605 + +1.5 667,605 + +0.3 667,605 + +1.3 667,605 + +0.8 667,605 + +1.8 667,605 + +1.5 667,605 + +0.9 667,605 + +0.1 667,605 + +0.1 667,605 + -1.9 667,605 + +0.4 667,605 + +0.3 667,605 + +1.6 667,605 + +1.3 667,605 + +0.9 667,606 - +0.6 667,606 - +1.7 667,606 - +0.9 667,606 - +3.1 667,606 - +1.2 667,606 - -0.7 667,606 - +0.2 667,606 - +0.7 667,606 - +1.2 667,608 - -0.1 667,662 + +1.3 667,662 + +0.7 667,662 + +2.7 667,680 + -0.3 667,680 + +0.4 667,680 + +0.3 667,680 + +0.1 667,680 + +2.2 667,680 + -0.2 667,680 + +1.8 667,680 + -0.1 667,680 + +0.1 667,680 + +0.6 667,680 + -0.1 667,681 - +1.9 667,681 - -0.1 667,683 + +0.8 667,683 + -0.6 667,683 + +0.9 667,715 + -0.5 667,715 + +0.9 667,715 + +2.5 667,715 + +0.2 667,715 + +1.6 667,715 + +0.2 667,715 + -0.1 667,715 + +0.9 667,715 + +0.6 667,715 + +0.1 667,715 + +0.7 667,715 + +1.4 667,715 + -0.1 667,715 + +0.4 667,715 + +1.7 667,715 + +0.0 667,716 - -0.1 667,720 + +1.1 667,720 + +0.3 667,720 + +1.9 667,720 + -1.6 667,720 + +4.4 667,720 + +0.2 667,720 + +1.5 667,720 + +0.3 667,751 + +0.4 667,751 + +1.4 667,751 + +1.0 667,751 + +0.6 667,751 + +0.9 667,751 + +0.8 667,751 + +1.5 667,751 + -1.7 667,751 + +1.1 667,774 + +2.1 667,793 + -0.5 667,793 + -0.1 667,795 + -0.7 667,795 + -0.2 667,795 + +0.3 667,795 + -1.4 667,796 - -0.1 667,796 - +2.3 667,825 + -1.4 667,826 - +0.5 667,849 + +0.3 667,849 + -1.1 667,849 + +1.2 667,891 + -0.7 667,891 + -0.9 667,891 + -0.1 667,957 + -1.7 667,957 + -0.5 667,957 + +0.3 667,958 - -1.1 668,105 - Pf1N1B4_574 0.53 -0.7 668,128 + Pf1N1B4_574 0.66 -0.7 668,128 + Pf1N1B4_574 0.66 -0.8 668,809 + Pf1N1B4_575 0.41 -0.0 668,809 + Pf1N1B4_575 0.41 +0.9 668,889 + +1.7 668,889 + Pf1N1B4_575 0.47 -1.1 668,889 + Pf1N1B4_575 0.47 +1.9 668,889 + Pf1N1B4_575 0.47 +1.3 668,889 + Pf1N1B4_575 0.47 -0.1 668,889 + Pf1N1B4_575 0.47 -2.7 668,889 + Pf1N1B4_575 0.47 +0.9 668,889 + Pf1N1B4_575 0.47 +0.9 668,889 + Pf1N1B4_575 0.47 -1.4 668,889 + Pf1N1B4_575 0.47 +0.4 668,889 + Pf1N1B4_575 0.47 +0.9 668,889 + Pf1N1B4_575 0.47 +0.5 668,889 + Pf1N1B4_575 0.47 -1.7 668,889 + Pf1N1B4_575 0.47 -1.4 668,889 + Pf1N1B4_575 0.47 +0.9 668,890 - Pf1N1B4_575 0.47 +0.0 668,890 - Pf1N1B4_575 0.47 +2.7 668,890 - Pf1N1B4_575 0.47 +0.1 668,890 - Pf1N1B4_575 0.47 +0.7 668,890 - Pf1N1B4_575 0.47 -1.2 668,890 - -0.7 668,890 - Pf1N1B4_575 0.47 +0.9 668,893 + Pf1N1B4_575 0.47 -2.1 668,893 + Pf1N1B4_575 0.47 -2.4 668,893 + Pf1N1B4_575 0.47 +1.3 668,893 + Pf1N1B4_575 0.47 -0.7 668,893 + Pf1N1B4_575 0.47 -1.9 668,894 - Pf1N1B4_575 0.47 +0.9 668,894 - Pf1N1B4_575 0.47 +0.3 668,894 - Pf1N1B4_575 0.47 +0.4 668,894 - +0.9 668,894 - Pf1N1B4_575 0.47 -0.3 668,894 - Pf1N1B4_575 0.47 -0.5 668,894 - Pf1N1B4_575 0.47 +0.3 668,894 - Pf1N1B4_575 0.47 -0.1 668,894 - Pf1N1B4_575 0.47 +0.4 668,894 - Pf1N1B4_575 0.47 -0.3 668,894 - Pf1N1B4_575 0.47 -1.4 668,894 - Pf1N1B4_575 0.47 -0.9 668,894 - Pf1N1B4_575 0.47 +1.5 668,899 - Pf1N1B4_575 0.47 +0.3 668,899 - Pf1N1B4_575 0.47 -1.4 668,899 - Pf1N1B4_575 0.47 +1.2 669,060 + Pf1N1B4_575 0.58 +0.3 669,149 - Pf1N1B4_575 0.64 -0.9 669,149 - Pf1N1B4_575 0.64 -0.1 669,149 - Pf1N1B4_575 0.64 -0.1 669,166 - Pf1N1B4_575 0.65 +0.2 669,166 - Pf1N1B4_575 0.65 -1.5
Or see this region's nucleotide sequence