Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5591 and Pf1N1B4_5592 are separated by 15 nucleotides Pf1N1B4_5592 and Pf1N1B4_5593 overlap by 32 nucleotides Pf1N1B4_5593 and Pf1N1B4_5594 are separated by 212 nucleotides Pf1N1B4_5594 and Pf1N1B4_5595 are separated by 57 nucleotides
Pf1N1B4_5591 - Vanillin dehydrogenase (Hydroxybenzaldehyde dehydrogenase) (EC 1.2.1.28), at 13,810 to 15,258
_5591
Pf1N1B4_5592 - hypothetical protein, at 15,274 to 15,417
_5592
Pf1N1B4_5593 - 4-hydroxycinnamoyl CoA hydratase/lyase (Enoyl-CoA hydratase/lyase) (EC 4.2.1.17), at 15,386 to 16,216
_5593
Pf1N1B4_5594 - Transcriptional regulator, MarR family, at 16,429 to 16,917
_5594
Pf1N1B4_5595 - Phenylacetic acid-specific porin; hydroxycinnamate specific porine, at 16,975 to 18,213
_5595
Position (kb) 15
16
17 Strain fitness (log2 ratio) -2
-1
0
1 at 14.878 kb on + strand, within Pf1N1B4_5591 at 14.878 kb on + strand at 14.878 kb on + strand, within Pf1N1B4_5591 at 14.879 kb on - strand, within Pf1N1B4_5591 at 15.273 kb on - strand at 15.291 kb on - strand, within Pf1N1B4_5592 at 15.508 kb on - strand, within Pf1N1B4_5593 at 15.708 kb on + strand, within Pf1N1B4_5593 at 15.708 kb on + strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.903 kb on + strand, within Pf1N1B4_5593 at 15.904 kb on - strand, within Pf1N1B4_5593 at 15.993 kb on + strand, within Pf1N1B4_5593 at 15.994 kb on - strand, within Pf1N1B4_5593 at 15.994 kb on - strand, within Pf1N1B4_5593 at 15.994 kb on - strand, within Pf1N1B4_5593 at 16.206 kb on + strand at 16.976 kb on + strand at 16.977 kb on - strand at 17.118 kb on - strand, within Pf1N1B4_5595 at 17.118 kb on - strand at 17.198 kb on + strand at 17.198 kb on + strand, within Pf1N1B4_5595 at 17.198 kb on + strand, within Pf1N1B4_5595 at 17.199 kb on - strand, within Pf1N1B4_5595 at 17.199 kb on - strand, within Pf1N1B4_5595 at 17.199 kb on - strand, within Pf1N1B4_5595
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 14,878 + Pf1N1B4_5591 0.74 +1.1 14,878 + -0.5 14,878 + Pf1N1B4_5591 0.74 +1.0 14,879 - Pf1N1B4_5591 0.74 +0.1 15,273 - -1.5 15,291 - Pf1N1B4_5592 0.12 -0.8 15,508 - Pf1N1B4_5593 0.15 -1.8 15,708 + Pf1N1B4_5593 0.39 -0.7 15,708 + Pf1N1B4_5593 0.39 -0.3 15,709 - Pf1N1B4_5593 0.39 +0.2 15,709 - Pf1N1B4_5593 0.39 +0.1 15,709 - Pf1N1B4_5593 0.39 +0.3 15,709 - Pf1N1B4_5593 0.39 +0.2 15,709 - Pf1N1B4_5593 0.39 +0.8 15,903 + Pf1N1B4_5593 0.62 +0.9 15,904 - Pf1N1B4_5593 0.62 +0.1 15,993 + Pf1N1B4_5593 0.73 +0.9 15,994 - Pf1N1B4_5593 0.73 -1.5 15,994 - Pf1N1B4_5593 0.73 +1.5 15,994 - Pf1N1B4_5593 0.73 +0.8 16,206 + +1.1 16,976 + -0.3 16,977 - +0.0 17,118 - Pf1N1B4_5595 0.12 -1.5 17,118 - +0.5 17,198 + +1.1 17,198 + Pf1N1B4_5595 0.18 -0.5 17,198 + Pf1N1B4_5595 0.18 +0.8 17,199 - Pf1N1B4_5595 0.18 -0.5 17,199 - Pf1N1B4_5595 0.18 +1.2 17,199 - Pf1N1B4_5595 0.18 -0.2
Or see this region's nucleotide sequence