Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5513

Experiment: Choline Lysine 6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5511 and Pf1N1B4_5512 are separated by 342 nucleotidesPf1N1B4_5512 and Pf1N1B4_5513 are separated by 75 nucleotidesPf1N1B4_5513 and Pf1N1B4_5514 are separated by 69 nucleotides Pf1N1B4_5511 - hypothetical protein, at 260,474 to 260,755 _5511 Pf1N1B4_5512 - hypothetical protein, at 261,098 to 261,244 _5512 Pf1N1B4_5513 - hypothetical protein, at 261,320 to 261,517 _5513 Pf1N1B4_5514 - RecA protein, at 261,587 to 262,636 _5514 Position (kb) 261 262Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 260.383 kb on + strandat 260.384 kb on - strandat 260.408 kb on + strandat 260.413 kb on + strandat 260.413 kb on + strandat 260.413 kb on + strandat 260.413 kb on + strandat 260.414 kb on - strandat 260.414 kb on - strandat 260.414 kb on - strandat 260.414 kb on - strandat 260.414 kb on - strandat 260.414 kb on - strandat 260.414 kb on - strandat 260.414 kb on - strandat 260.476 kb on - strandat 260.476 kb on - strandat 260.476 kb on - strandat 260.499 kb on + strandat 260.499 kb on + strandat 260.500 kb on - strandat 260.510 kb on + strand, within Pf1N1B4_5511at 260.511 kb on - strand, within Pf1N1B4_5511at 260.511 kb on - strand, within Pf1N1B4_5511at 260.511 kb on - strand, within Pf1N1B4_5511at 260.511 kb on - strand, within Pf1N1B4_5511at 260.575 kb on - strand, within Pf1N1B4_5511at 260.639 kb on + strand, within Pf1N1B4_5511at 260.639 kb on + strand, within Pf1N1B4_5511at 260.640 kb on - strand, within Pf1N1B4_5511at 260.640 kb on - strand, within Pf1N1B4_5511at 260.691 kb on - strand, within Pf1N1B4_5511at 260.786 kb on + strandat 260.786 kb on + strandat 260.787 kb on - strandat 260.787 kb on - strandat 260.787 kb on - strandat 260.787 kb on - strandat 260.790 kb on + strandat 260.920 kb on + strandat 260.920 kb on + strandat 261.018 kb on + strandat 261.018 kb on + strandat 261.164 kb on - strand, within Pf1N1B4_5512at 261.164 kb on - strand, within Pf1N1B4_5512at 261.166 kb on - strand, within Pf1N1B4_5512at 261.222 kb on - strand, within Pf1N1B4_5512at 261.260 kb on + strandat 261.260 kb on + strandat 261.261 kb on - strandat 261.261 kb on - strandat 261.265 kb on + strandat 261.265 kb on + strandat 261.266 kb on - strandat 261.266 kb on - strandat 261.287 kb on - strandat 261.287 kb on - strandat 261.296 kb on - strandat 261.316 kb on + strandat 261.316 kb on + strandat 261.317 kb on - strandat 261.317 kb on - strandat 261.321 kb on + strandat 261.321 kb on + strandat 261.321 kb on + strandat 261.322 kb on - strandat 261.322 kb on - strandat 261.322 kb on - strandat 261.322 kb on - strandat 261.458 kb on + strand, within Pf1N1B4_5513at 261.458 kb on + strand, within Pf1N1B4_5513at 261.459 kb on - strand, within Pf1N1B4_5513at 261.459 kb on - strand, within Pf1N1B4_5513at 261.459 kb on - strand, within Pf1N1B4_5513at 261.468 kb on + strand, within Pf1N1B4_5513at 261.534 kb on + strandat 261.535 kb on - strandat 261.550 kb on - strandat 261.555 kb on - strandat 261.668 kb on - strandat 261.712 kb on - strand, within Pf1N1B4_5514at 261.712 kb on - strand, within Pf1N1B4_5514at 261.712 kb on - strand, within Pf1N1B4_5514at 261.712 kb on - strand, within Pf1N1B4_5514at 261.712 kb on - strand, within Pf1N1B4_5514at 261.712 kb on - strand, within Pf1N1B4_5514at 261.712 kb on - strand, within Pf1N1B4_5514at 261.756 kb on + strand, within Pf1N1B4_5514at 261.757 kb on - strand, within Pf1N1B4_5514at 261.757 kb on - strand, within Pf1N1B4_5514at 261.757 kb on - strand, within Pf1N1B4_5514at 261.757 kb on - strand, within Pf1N1B4_5514at 261.757 kb on - strand, within Pf1N1B4_5514at 261.757 kb on - strand, within Pf1N1B4_5514at 261.757 kb on - strand, within Pf1N1B4_5514at 261.763 kb on - strand, within Pf1N1B4_5514at 261.843 kb on + strand, within Pf1N1B4_5514at 261.843 kb on + strand, within Pf1N1B4_5514at 261.844 kb on - strand, within Pf1N1B4_5514at 261.844 kb on - strand, within Pf1N1B4_5514at 261.844 kb on - strand, within Pf1N1B4_5514at 261.844 kb on - strand, within Pf1N1B4_5514at 261.844 kb on - strand, within Pf1N1B4_5514at 261.847 kb on + strandat 261.848 kb on - strand, within Pf1N1B4_5514at 261.848 kb on - strand, within Pf1N1B4_5514at 261.848 kb on - strand, within Pf1N1B4_5514at 261.848 kb on - strand, within Pf1N1B4_5514at 261.981 kb on + strand, within Pf1N1B4_5514at 261.981 kb on + strand, within Pf1N1B4_5514at 261.981 kb on + strand, within Pf1N1B4_5514at 261.981 kb on + strand, within Pf1N1B4_5514at 261.981 kb on + strand, within Pf1N1B4_5514at 261.981 kb on + strand, within Pf1N1B4_5514at 261.982 kb on - strand, within Pf1N1B4_5514at 261.982 kb on - strand, within Pf1N1B4_5514at 261.982 kb on - strand, within Pf1N1B4_5514at 261.982 kb on - strand, within Pf1N1B4_5514at 262.307 kb on + strand, within Pf1N1B4_5514at 262.308 kb on - strand, within Pf1N1B4_5514at 262.326 kb on + strand, within Pf1N1B4_5514

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 6 mM
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260,383 + +0.1
260,384 - +0.3
260,408 + +0.9
260,413 + +0.6
260,413 + -2.2
260,413 + +1.1
260,413 + +0.8
260,414 - +0.1
260,414 - -0.3
260,414 - -1.1
260,414 - +1.6
260,414 - -0.5
260,414 - +2.7
260,414 - +1.1
260,414 - +0.1
260,476 - -0.3
260,476 - +2.1
260,476 - +0.1
260,499 + +1.1
260,499 + -0.2
260,500 - +0.0
260,510 + Pf1N1B4_5511 0.13 -0.9
260,511 - Pf1N1B4_5511 0.13 +1.1
260,511 - Pf1N1B4_5511 0.13 -1.4
260,511 - Pf1N1B4_5511 0.13 +1.7
260,511 - Pf1N1B4_5511 0.13 +0.1
260,575 - Pf1N1B4_5511 0.36 +0.7
260,639 + Pf1N1B4_5511 0.59 -1.0
260,639 + Pf1N1B4_5511 0.59 +0.1
260,640 - Pf1N1B4_5511 0.59 -0.8
260,640 - Pf1N1B4_5511 0.59 +1.2
260,691 - Pf1N1B4_5511 0.77 -0.9
260,786 + -0.2
260,786 + -0.4
260,787 - +0.9
260,787 - -0.4
260,787 - -0.6
260,787 - -2.2
260,790 + -1.0
260,920 + -0.1
260,920 + -0.9
261,018 + -0.5
261,018 + -0.6
261,164 - Pf1N1B4_5512 0.45 +1.4
261,164 - Pf1N1B4_5512 0.45 -0.4
261,166 - Pf1N1B4_5512 0.46 -0.9
261,222 - Pf1N1B4_5512 0.84 -1.2
261,260 + +1.1
261,260 + +0.1
261,261 - -0.9
261,261 - -0.9
261,265 + +1.8
261,265 + +2.7
261,266 - -0.6
261,266 - +0.1
261,287 - +0.6
261,287 - +0.3
261,296 - -0.3
261,316 + -0.8
261,316 + +1.0
261,317 - -3.1
261,317 - +0.9
261,321 + +0.1
261,321 + +0.1
261,321 + +0.6
261,322 - -0.4
261,322 - -2.4
261,322 - -0.8
261,322 - +0.1
261,458 + Pf1N1B4_5513 0.70 -0.6
261,458 + Pf1N1B4_5513 0.70 -0.6
261,459 - Pf1N1B4_5513 0.70 +0.1
261,459 - Pf1N1B4_5513 0.70 -0.8
261,459 - Pf1N1B4_5513 0.70 +0.1
261,468 + Pf1N1B4_5513 0.75 -1.5
261,534 + -0.3
261,535 - +1.1
261,550 - +1.4
261,555 - +0.1
261,668 - +1.0
261,712 - Pf1N1B4_5514 0.12 -0.6
261,712 - Pf1N1B4_5514 0.12 +2.5
261,712 - Pf1N1B4_5514 0.12 -0.9
261,712 - Pf1N1B4_5514 0.12 +0.3
261,712 - Pf1N1B4_5514 0.12 +0.9
261,712 - Pf1N1B4_5514 0.12 +0.1
261,712 - Pf1N1B4_5514 0.12 +0.1
261,756 + Pf1N1B4_5514 0.16 -2.3
261,757 - Pf1N1B4_5514 0.16 +1.1
261,757 - Pf1N1B4_5514 0.16 +0.3
261,757 - Pf1N1B4_5514 0.16 +1.7
261,757 - Pf1N1B4_5514 0.16 +0.4
261,757 - Pf1N1B4_5514 0.16 +0.0
261,757 - Pf1N1B4_5514 0.16 -0.4
261,757 - Pf1N1B4_5514 0.16 -0.2
261,763 - Pf1N1B4_5514 0.17 +0.2
261,843 + Pf1N1B4_5514 0.24 -0.7
261,843 + Pf1N1B4_5514 0.24 -0.5
261,844 - Pf1N1B4_5514 0.24 +0.1
261,844 - Pf1N1B4_5514 0.24 +1.7
261,844 - Pf1N1B4_5514 0.24 +0.6
261,844 - Pf1N1B4_5514 0.24 +0.3
261,844 - Pf1N1B4_5514 0.24 +2.1
261,847 + -0.2
261,848 - Pf1N1B4_5514 0.25 -0.2
261,848 - Pf1N1B4_5514 0.25 -1.2
261,848 - Pf1N1B4_5514 0.25 +0.5
261,848 - Pf1N1B4_5514 0.25 -0.9
261,981 + Pf1N1B4_5514 0.38 +1.6
261,981 + Pf1N1B4_5514 0.38 +1.1
261,981 + Pf1N1B4_5514 0.38 -0.4
261,981 + Pf1N1B4_5514 0.38 -0.4
261,981 + Pf1N1B4_5514 0.38 +1.9
261,981 + Pf1N1B4_5514 0.38 +1.1
261,982 - Pf1N1B4_5514 0.38 -1.2
261,982 - Pf1N1B4_5514 0.38 +0.4
261,982 - Pf1N1B4_5514 0.38 -1.3
261,982 - Pf1N1B4_5514 0.38 -0.1
262,307 + Pf1N1B4_5514 0.69 +0.7
262,308 - Pf1N1B4_5514 0.69 +0.7
262,326 + Pf1N1B4_5514 0.70 +2.1

Or see this region's nucleotide sequence