Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5406 and Pf1N1B4_5407 are separated by 379 nucleotides Pf1N1B4_5407 and Pf1N1B4_5408 are separated by 69 nucleotides Pf1N1B4_5408 and Pf1N1B4_5409 are separated by 38 nucleotides Pf1N1B4_5409 and Pf1N1B4_5410 are separated by 553 nucleotides
Pf1N1B4_5406 - hypothetical protein, at 142,057 to 142,173
_5406
Pf1N1B4_5407 - hypothetical protein, at 142,553 to 142,762
_5407
Pf1N1B4_5408 - hypothetical protein, at 142,832 to 143,065
_5408
Pf1N1B4_5409 - hypothetical protein, at 143,104 to 143,562
_5409
Pf1N1B4_5410 - hypothetical protein, at 144,116 to 146,206
_5410
Position (kb)
143
144 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 142.268 kb on + strand at 142.269 kb on - strand at 142.269 kb on - strand at 142.357 kb on + strand at 142.357 kb on + strand at 142.358 kb on - strand at 142.452 kb on - strand at 142.523 kb on - strand at 142.593 kb on + strand, within Pf1N1B4_5407 at 142.665 kb on + strand, within Pf1N1B4_5407 at 142.665 kb on + strand, within Pf1N1B4_5407 at 142.666 kb on - strand, within Pf1N1B4_5407 at 142.666 kb on - strand, within Pf1N1B4_5407 at 142.666 kb on - strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.767 kb on - strand at 142.767 kb on - strand at 142.819 kb on - strand at 142.877 kb on + strand, within Pf1N1B4_5408 at 142.877 kb on + strand, within Pf1N1B4_5408 at 142.877 kb on + strand, within Pf1N1B4_5408 at 142.878 kb on - strand, within Pf1N1B4_5408 at 142.878 kb on - strand, within Pf1N1B4_5408 at 142.878 kb on - strand at 142.879 kb on + strand, within Pf1N1B4_5408 at 142.879 kb on + strand at 142.880 kb on - strand, within Pf1N1B4_5408 at 142.982 kb on - strand, within Pf1N1B4_5408 at 142.989 kb on + strand, within Pf1N1B4_5408 at 142.989 kb on + strand, within Pf1N1B4_5408 at 142.990 kb on - strand, within Pf1N1B4_5408 at 143.064 kb on - strand at 143.081 kb on + strand at 143.134 kb on - strand at 143.134 kb on - strand at 143.134 kb on - strand at 143.169 kb on - strand, within Pf1N1B4_5409 at 143.180 kb on + strand at 143.181 kb on - strand, within Pf1N1B4_5409 at 143.181 kb on - strand, within Pf1N1B4_5409 at 143.181 kb on - strand at 143.192 kb on + strand, within Pf1N1B4_5409 at 143.192 kb on + strand, within Pf1N1B4_5409 at 143.192 kb on + strand, within Pf1N1B4_5409 at 143.192 kb on + strand at 143.193 kb on - strand, within Pf1N1B4_5409 at 143.193 kb on - strand, within Pf1N1B4_5409 at 143.373 kb on - strand, within Pf1N1B4_5409 at 143.373 kb on - strand, within Pf1N1B4_5409 at 143.373 kb on - strand, within Pf1N1B4_5409 at 143.413 kb on - strand, within Pf1N1B4_5409 at 143.436 kb on - strand, within Pf1N1B4_5409 at 143.436 kb on - strand, within Pf1N1B4_5409 at 143.440 kb on + strand, within Pf1N1B4_5409 at 143.440 kb on + strand, within Pf1N1B4_5409 at 143.441 kb on - strand, within Pf1N1B4_5409 at 143.441 kb on - strand, within Pf1N1B4_5409 at 143.441 kb on - strand at 143.441 kb on - strand, within Pf1N1B4_5409 at 143.441 kb on - strand at 143.441 kb on - strand, within Pf1N1B4_5409 at 143.441 kb on - strand at 143.441 kb on - strand, within Pf1N1B4_5409 at 143.441 kb on - strand, within Pf1N1B4_5409 at 143.444 kb on + strand, within Pf1N1B4_5409 at 143.445 kb on - strand, within Pf1N1B4_5409 at 143.445 kb on - strand, within Pf1N1B4_5409 at 143.466 kb on - strand, within Pf1N1B4_5409 at 143.525 kb on + strand at 143.525 kb on + strand at 143.526 kb on - strand at 143.562 kb on + strand at 143.562 kb on + strand at 143.562 kb on + strand at 143.562 kb on + strand at 143.625 kb on - strand at 143.649 kb on + strand at 143.671 kb on + strand at 143.672 kb on - strand at 143.680 kb on - strand at 143.747 kb on + strand at 143.747 kb on + strand at 143.747 kb on + strand at 143.747 kb on + strand at 143.747 kb on + strand at 143.747 kb on + strand at 143.748 kb on - strand at 143.748 kb on - strand at 143.748 kb on - strand at 143.748 kb on - strand at 143.748 kb on - strand at 143.748 kb on - strand at 143.748 kb on - strand at 143.748 kb on - strand at 143.748 kb on - strand at 143.748 kb on - strand at 143.748 kb on - strand at 143.748 kb on - strand at 143.765 kb on + strand at 143.765 kb on + strand at 143.765 kb on + strand at 143.766 kb on - strand at 143.766 kb on - strand at 143.788 kb on + strand at 143.788 kb on + strand at 143.788 kb on + strand at 143.788 kb on + strand at 143.788 kb on + strand at 143.788 kb on + strand at 143.788 kb on + strand at 143.788 kb on + strand at 143.788 kb on + strand at 143.789 kb on - strand at 143.789 kb on - strand at 143.789 kb on - strand at 143.789 kb on - strand at 143.789 kb on - strand at 143.789 kb on - strand at 143.789 kb on - strand at 143.789 kb on - strand at 143.789 kb on - strand at 143.792 kb on + strand at 143.792 kb on + strand at 143.793 kb on - strand at 143.809 kb on + strand at 143.809 kb on + strand at 143.817 kb on + strand at 143.818 kb on - strand at 143.909 kb on + strand at 143.909 kb on + strand at 143.922 kb on + strand at 143.943 kb on + strand at 143.943 kb on + strand at 143.943 kb on + strand at 143.943 kb on + strand at 143.943 kb on + strand at 143.943 kb on + strand at 143.944 kb on - strand at 143.944 kb on - strand at 143.944 kb on - strand at 143.944 kb on - strand at 143.944 kb on - strand at 144.039 kb on - strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.046 kb on + strand at 144.047 kb on - strand at 144.047 kb on - strand at 144.047 kb on - strand at 144.047 kb on - strand at 144.047 kb on - strand at 144.047 kb on - strand at 144.047 kb on - strand at 144.047 kb on - strand at 144.047 kb on - strand at 144.047 kb on - strand at 144.047 kb on - strand at 144.068 kb on - strand at 144.086 kb on + strand at 144.087 kb on - strand at 144.263 kb on - strand at 144.298 kb on + strand at 144.311 kb on + strand at 144.312 kb on - strand at 144.323 kb on - strand at 144.362 kb on + strand, within Pf1N1B4_5410 at 144.362 kb on + strand, within Pf1N1B4_5410 at 144.363 kb on - strand at 144.363 kb on - strand, within Pf1N1B4_5410 at 144.364 kb on + strand, within Pf1N1B4_5410 at 144.365 kb on - strand, within Pf1N1B4_5410 at 144.365 kb on - strand at 144.365 kb on - strand, within Pf1N1B4_5410 at 144.458 kb on - strand at 144.458 kb on - strand, within Pf1N1B4_5410 at 144.458 kb on - strand, within Pf1N1B4_5410 at 144.470 kb on - strand, within Pf1N1B4_5410
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 142,268 + -1.8 142,269 - -0.2 142,269 - +0.6 142,357 + +2.2 142,357 + -0.0 142,358 - +0.2 142,452 - -1.4 142,523 - +1.2 142,593 + Pf1N1B4_5407 0.19 +0.2 142,665 + Pf1N1B4_5407 0.53 -0.2 142,665 + Pf1N1B4_5407 0.53 -0.2 142,666 - Pf1N1B4_5407 0.54 -0.4 142,666 - Pf1N1B4_5407 0.54 +0.8 142,666 - +0.6 142,766 + +1.2 142,766 + +1.2 142,766 + +0.4 142,766 + +0.3 142,766 + -0.2 142,766 + -0.2 142,767 - +0.2 142,767 - -2.5 142,819 - -0.8 142,877 + Pf1N1B4_5408 0.19 +0.6 142,877 + Pf1N1B4_5408 0.19 -1.4 142,877 + Pf1N1B4_5408 0.19 -2.2 142,878 - Pf1N1B4_5408 0.20 -1.3 142,878 - Pf1N1B4_5408 0.20 +0.6 142,878 - +0.2 142,879 + Pf1N1B4_5408 0.20 -0.7 142,879 + +1.9 142,880 - Pf1N1B4_5408 0.21 +2.0 142,982 - Pf1N1B4_5408 0.64 -0.2 142,989 + Pf1N1B4_5408 0.67 -0.2 142,989 + Pf1N1B4_5408 0.67 +0.3 142,990 - Pf1N1B4_5408 0.68 +0.3 143,064 - +1.6 143,081 + +0.9 143,134 - -0.2 143,134 - +0.2 143,134 - +0.1 143,169 - Pf1N1B4_5409 0.14 -1.4 143,180 + -1.6 143,181 - Pf1N1B4_5409 0.17 +1.2 143,181 - Pf1N1B4_5409 0.17 +1.2 143,181 - -1.6 143,192 + Pf1N1B4_5409 0.19 +0.9 143,192 + Pf1N1B4_5409 0.19 +0.8 143,192 + Pf1N1B4_5409 0.19 -1.2 143,192 + +1.2 143,193 - Pf1N1B4_5409 0.19 -1.2 143,193 - Pf1N1B4_5409 0.19 -0.2 143,373 - Pf1N1B4_5409 0.59 +0.6 143,373 - Pf1N1B4_5409 0.59 +0.8 143,373 - Pf1N1B4_5409 0.59 -1.5 143,413 - Pf1N1B4_5409 0.67 +1.4 143,436 - Pf1N1B4_5409 0.72 -0.3 143,436 - Pf1N1B4_5409 0.72 -0.1 143,440 + Pf1N1B4_5409 0.73 -0.2 143,440 + Pf1N1B4_5409 0.73 +2.2 143,441 - Pf1N1B4_5409 0.73 -1.2 143,441 - Pf1N1B4_5409 0.73 +0.6 143,441 - +2.2 143,441 - Pf1N1B4_5409 0.73 +0.4 143,441 - +2.8 143,441 - Pf1N1B4_5409 0.73 +1.5 143,441 - -0.8 143,441 - Pf1N1B4_5409 0.73 -0.6 143,441 - Pf1N1B4_5409 0.73 +0.8 143,444 + Pf1N1B4_5409 0.74 +1.2 143,445 - Pf1N1B4_5409 0.74 -0.6 143,445 - Pf1N1B4_5409 0.74 +1.3 143,466 - Pf1N1B4_5409 0.79 -1.0 143,525 + -0.6 143,525 + -0.8 143,526 - +0.2 143,562 + -0.4 143,562 + -0.4 143,562 + +1.2 143,562 + -1.2 143,625 - +0.3 143,649 + +0.4 143,671 + +0.4 143,672 - -2.2 143,680 - -1.4 143,747 + -1.4 143,747 + -0.2 143,747 + +0.6 143,747 + +0.8 143,747 + -1.0 143,747 + -0.4 143,748 - +0.8 143,748 - +0.1 143,748 - +1.0 143,748 - -0.9 143,748 - -0.1 143,748 - +0.9 143,748 - -0.4 143,748 - -0.0 143,748 - +0.4 143,748 - -0.1 143,748 - +1.4 143,748 - +1.0 143,765 + +0.6 143,765 + -1.8 143,765 + +2.5 143,766 - +1.5 143,766 - -0.1 143,788 + +1.2 143,788 + -0.4 143,788 + -1.4 143,788 + +0.4 143,788 + +0.3 143,788 + -0.6 143,788 + -0.2 143,788 + -0.2 143,788 + +0.4 143,789 - -0.7 143,789 - -1.0 143,789 - -0.8 143,789 - +1.2 143,789 - -0.2 143,789 - -0.1 143,789 - +0.6 143,789 - +0.9 143,789 - -0.8 143,792 + +0.4 143,792 + -0.3 143,793 - +1.8 143,809 + +0.4 143,809 + +0.3 143,817 + +0.7 143,818 - -2.7 143,909 + +0.2 143,909 + -0.8 143,922 + -0.4 143,943 + +0.2 143,943 + -0.8 143,943 + +0.4 143,943 + -1.4 143,943 + -0.2 143,943 + +0.5 143,944 - -1.8 143,944 - +1.2 143,944 - +0.9 143,944 - +0.4 143,944 - -1.4 144,039 - +1.4 144,046 + -2.0 144,046 + +1.2 144,046 + -1.2 144,046 + +0.4 144,046 + +1.0 144,046 + -0.6 144,046 + -1.2 144,046 + -0.8 144,046 + +0.9 144,046 + +0.2 144,046 + +1.2 144,046 + +0.3 144,046 + +0.2 144,046 + +2.2 144,046 + -1.1 144,046 + -0.1 144,046 + -0.2 144,046 + +0.4 144,046 + +0.8 144,047 - -0.4 144,047 - +2.0 144,047 - +2.5 144,047 - -0.4 144,047 - +1.5 144,047 - -0.4 144,047 - +0.0 144,047 - -0.8 144,047 - -1.2 144,047 - -0.8 144,047 - +1.2 144,068 - +2.0 144,086 + +0.2 144,087 - +1.8 144,263 - +0.9 144,298 + +0.6 144,311 + +0.7 144,312 - +0.0 144,323 - +0.2 144,362 + Pf1N1B4_5410 0.12 +0.2 144,362 + Pf1N1B4_5410 0.12 +0.2 144,363 - -0.8 144,363 - Pf1N1B4_5410 0.12 -1.2 144,364 + Pf1N1B4_5410 0.12 +1.4 144,365 - Pf1N1B4_5410 0.12 -1.6 144,365 - +2.2 144,365 - Pf1N1B4_5410 0.12 -0.4 144,458 - -0.4 144,458 - Pf1N1B4_5410 0.16 +0.7 144,458 - Pf1N1B4_5410 0.16 +1.2 144,470 - Pf1N1B4_5410 0.17 +1.0
Or see this region's nucleotide sequence