Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5330 and Pf1N1B4_5331 are separated by 356 nucleotides Pf1N1B4_5331 and Pf1N1B4_5332 are separated by 2 nucleotides Pf1N1B4_5332 and Pf1N1B4_5333 are separated by 11 nucleotides Pf1N1B4_5333 and Pf1N1B4_5334 are separated by 131 nucleotides Pf1N1B4_5334 and Pf1N1B4_5335 are separated by 181 nucleotides
Pf1N1B4_5330 - hypothetical protein, at 77,922 to 78,233
_5330
Pf1N1B4_5331 - hypothetical protein, at 78,590 to 78,760
_5331
Pf1N1B4_5332 - hypothetical protein, at 78,763 to 78,957
_5332
Pf1N1B4_5333 - hypothetical protein, at 78,969 to 79,595
_5333
Pf1N1B4_5334 - hypothetical protein, at 79,727 to 79,930
_5334
Pf1N1B4_5335 - YebG, DNA damage-inducible gene in SOS regulon, expressed in stationary phase, at 80,112 to 80,399
_5335
Position (kb)
78
79
80 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 77.980 kb on + strand, within Pf1N1B4_5330 at 78.022 kb on - strand, within Pf1N1B4_5330 at 78.152 kb on + strand at 78.152 kb on + strand, within Pf1N1B4_5330 at 78.153 kb on - strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.314 kb on - strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.352 kb on - strand at 78.352 kb on - strand at 78.392 kb on + strand at 78.392 kb on + strand at 78.425 kb on + strand at 78.425 kb on + strand at 78.426 kb on - strand at 78.426 kb on - strand at 78.474 kb on + strand at 78.474 kb on + strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.597 kb on - strand at 78.597 kb on - strand at 78.597 kb on - strand at 78.729 kb on + strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.739 kb on - strand, within Pf1N1B4_5331 at 78.971 kb on - strand at 79.045 kb on + strand, within Pf1N1B4_5333 at 79.045 kb on + strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.106 kb on + strand, within Pf1N1B4_5333 at 79.268 kb on + strand, within Pf1N1B4_5333 at 79.268 kb on + strand, within Pf1N1B4_5333 at 79.269 kb on - strand, within Pf1N1B4_5333 at 79.269 kb on - strand, within Pf1N1B4_5333 at 79.393 kb on - strand, within Pf1N1B4_5333 at 79.393 kb on - strand, within Pf1N1B4_5333 at 79.446 kb on - strand, within Pf1N1B4_5333 at 79.501 kb on + strand, within Pf1N1B4_5333 at 79.501 kb on + strand, within Pf1N1B4_5333 at 79.502 kb on - strand, within Pf1N1B4_5333 at 79.502 kb on - strand, within Pf1N1B4_5333 at 79.576 kb on + strand at 79.576 kb on + strand at 79.576 kb on + strand at 79.576 kb on + strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.580 kb on + strand at 79.580 kb on + strand at 79.726 kb on - strand at 79.728 kb on + strand at 79.793 kb on + strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.797 kb on + strand, within Pf1N1B4_5334 at 80.045 kb on + strand at 80.045 kb on + strand at 80.045 kb on + strand at 80.046 kb on - strand at 80.147 kb on + strand, within Pf1N1B4_5335 at 80.147 kb on + strand, within Pf1N1B4_5335 at 80.148 kb on - strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.228 kb on - strand, within Pf1N1B4_5335 at 80.228 kb on - strand, within Pf1N1B4_5335 at 80.228 kb on - strand at 80.228 kb on - strand, within Pf1N1B4_5335 at 80.348 kb on + strand, within Pf1N1B4_5335 at 80.348 kb on + strand, within Pf1N1B4_5335 at 80.349 kb on - strand, within Pf1N1B4_5335 at 80.349 kb on - strand, within Pf1N1B4_5335 at 80.349 kb on - strand, within Pf1N1B4_5335 at 80.380 kb on + strand at 80.584 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 77,980 + Pf1N1B4_5330 0.19 -0.2 78,022 - Pf1N1B4_5330 0.32 -1.8 78,152 + +1.8 78,152 + Pf1N1B4_5330 0.74 -0.4 78,153 - +4.3 78,313 + +0.6 78,313 + +1.4 78,313 + -1.2 78,313 + -0.3 78,314 - +0.2 78,336 + +1.2 78,336 + +0.7 78,336 + +0.6 78,336 + +0.7 78,351 + +1.8 78,351 + +2.5 78,351 + -0.4 78,352 - -0.6 78,352 - +1.2 78,392 + -1.8 78,392 + +1.8 78,425 + -1.2 78,425 + -1.6 78,426 - +0.9 78,426 - +0.7 78,474 + +1.2 78,474 + -0.8 78,475 - -1.2 78,475 - -0.4 78,475 - +0.2 78,475 - +0.2 78,475 - -0.8 78,597 - +1.2 78,597 - +1.6 78,597 - -0.4 78,729 + Pf1N1B4_5331 0.81 -1.4 78,730 - Pf1N1B4_5331 0.82 -1.1 78,730 - Pf1N1B4_5331 0.82 +1.0 78,730 - Pf1N1B4_5331 0.82 -1.2 78,739 - Pf1N1B4_5331 0.87 +0.1 78,971 - +1.2 79,045 + Pf1N1B4_5333 0.12 -1.8 79,045 + Pf1N1B4_5333 0.12 -1.4 79,046 - Pf1N1B4_5333 0.12 +1.2 79,046 - Pf1N1B4_5333 0.12 -3.1 79,046 - Pf1N1B4_5333 0.12 -1.3 79,106 + Pf1N1B4_5333 0.22 -0.6 79,268 + Pf1N1B4_5333 0.48 +1.2 79,268 + Pf1N1B4_5333 0.48 +0.4 79,269 - Pf1N1B4_5333 0.48 -0.6 79,269 - Pf1N1B4_5333 0.48 -1.2 79,393 - Pf1N1B4_5333 0.68 +0.8 79,393 - Pf1N1B4_5333 0.68 +0.0 79,446 - Pf1N1B4_5333 0.76 -0.4 79,501 + Pf1N1B4_5333 0.85 +0.4 79,501 + Pf1N1B4_5333 0.85 -2.9 79,502 - Pf1N1B4_5333 0.85 -0.7 79,502 - Pf1N1B4_5333 0.85 -2.0 79,576 + -2.0 79,576 + -0.2 79,576 + +1.6 79,576 + +1.2 79,577 - +1.6 79,577 - +0.4 79,577 - +0.7 79,577 - -0.5 79,577 - -0.4 79,580 + +1.3 79,580 + +2.7 79,726 - -0.4 79,728 + -0.4 79,793 + Pf1N1B4_5334 0.32 +0.4 79,794 - Pf1N1B4_5334 0.33 -0.2 79,794 - Pf1N1B4_5334 0.33 +0.4 79,794 - Pf1N1B4_5334 0.33 +0.8 79,794 - Pf1N1B4_5334 0.33 +0.5 79,797 + Pf1N1B4_5334 0.34 -2.0 80,045 + -0.6 80,045 + +0.2 80,045 + -1.2 80,046 - -0.4 80,147 + Pf1N1B4_5335 0.12 +0.2 80,147 + Pf1N1B4_5335 0.12 +1.2 80,148 - Pf1N1B4_5335 0.12 -0.4 80,227 + Pf1N1B4_5335 0.40 -0.4 80,227 + +1.2 80,227 + Pf1N1B4_5335 0.40 -0.0 80,227 + Pf1N1B4_5335 0.40 +0.4 80,227 + Pf1N1B4_5335 0.40 +0.2 80,227 + Pf1N1B4_5335 0.40 -0.2 80,227 + Pf1N1B4_5335 0.40 +0.9 80,227 + Pf1N1B4_5335 0.40 +2.2 80,227 + Pf1N1B4_5335 0.40 +0.8 80,227 + Pf1N1B4_5335 0.40 +1.9 80,227 + Pf1N1B4_5335 0.40 -0.8 80,228 - Pf1N1B4_5335 0.40 -0.1 80,228 - Pf1N1B4_5335 0.40 +0.7 80,228 - +1.6 80,228 - Pf1N1B4_5335 0.40 -1.2 80,348 + Pf1N1B4_5335 0.82 +0.1 80,348 + Pf1N1B4_5335 0.82 +0.7 80,349 - Pf1N1B4_5335 0.82 +0.6 80,349 - Pf1N1B4_5335 0.82 +0.4 80,349 - Pf1N1B4_5335 0.82 +0.4 80,380 + +0.3 80,584 - +1.2
Or see this region's nucleotide sequence