Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5320 and Pf1N1B4_5321 are separated by 969 nucleotides Pf1N1B4_5321 and Pf1N1B4_5322 are separated by 744 nucleotides
Pf1N1B4_5320 - hypothetical protein, at 69,546 to 69,671
_5320
Pf1N1B4_5321 - hypothetical protein, at 70,641 to 70,970
_5321
Pf1N1B4_5322 - hypothetical protein, at 71,715 to 71,900
_5322
Position (kb)
70
71 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 69.644 kb on - strand, within Pf1N1B4_5320 at 69.700 kb on + strand at 69.701 kb on - strand at 69.701 kb on - strand at 69.748 kb on - strand at 69.748 kb on - strand at 69.748 kb on - strand at 69.871 kb on + strand at 69.871 kb on + strand at 69.872 kb on - strand at 69.872 kb on - strand at 69.872 kb on - strand at 69.931 kb on - strand at 70.018 kb on + strand at 70.018 kb on + strand at 70.018 kb on + strand at 70.018 kb on + strand at 70.019 kb on - strand at 70.019 kb on - strand at 70.019 kb on - strand at 70.019 kb on - strand at 70.019 kb on - strand at 70.025 kb on + strand at 70.026 kb on - strand at 70.026 kb on - strand at 70.027 kb on + strand at 70.046 kb on - strand at 70.046 kb on - strand at 70.079 kb on + strand at 70.079 kb on + strand at 70.102 kb on - strand at 70.102 kb on - strand at 70.104 kb on + strand at 70.104 kb on + strand at 70.126 kb on + strand at 70.126 kb on + strand at 70.126 kb on + strand at 70.127 kb on - strand at 70.136 kb on + strand at 70.136 kb on + strand at 70.141 kb on + strand at 70.141 kb on + strand at 70.141 kb on + strand at 70.141 kb on + strand at 70.141 kb on + strand at 70.151 kb on - strand at 70.156 kb on + strand at 70.156 kb on + strand at 70.156 kb on + strand at 70.157 kb on - strand at 70.314 kb on - strand at 70.403 kb on + strand at 70.403 kb on + strand at 70.403 kb on + strand at 70.404 kb on - strand at 70.422 kb on + strand at 70.422 kb on + strand at 70.422 kb on + strand at 70.455 kb on + strand at 70.470 kb on + strand at 70.473 kb on + strand at 70.474 kb on - strand at 70.474 kb on - strand at 70.474 kb on - strand at 70.474 kb on - strand at 70.477 kb on + strand at 70.478 kb on - strand at 70.519 kb on + strand at 70.519 kb on + strand at 70.520 kb on - strand at 70.552 kb on + strand at 70.595 kb on + strand at 70.596 kb on - strand at 70.617 kb on + strand at 70.617 kb on + strand at 70.621 kb on + strand at 70.621 kb on + strand at 70.622 kb on - strand at 70.622 kb on - strand at 70.622 kb on - strand at 70.640 kb on + strand at 70.640 kb on + strand at 70.641 kb on - strand at 70.684 kb on - strand, within Pf1N1B4_5321 at 70.698 kb on + strand, within Pf1N1B4_5321 at 70.699 kb on - strand, within Pf1N1B4_5321 at 70.699 kb on - strand, within Pf1N1B4_5321 at 70.699 kb on - strand, within Pf1N1B4_5321 at 70.709 kb on + strand, within Pf1N1B4_5321 at 70.709 kb on + strand at 70.709 kb on + strand, within Pf1N1B4_5321 at 70.709 kb on + strand, within Pf1N1B4_5321 at 70.709 kb on + strand, within Pf1N1B4_5321 at 70.710 kb on - strand at 70.710 kb on - strand, within Pf1N1B4_5321 at 70.722 kb on - strand, within Pf1N1B4_5321 at 70.722 kb on - strand, within Pf1N1B4_5321 at 70.722 kb on - strand, within Pf1N1B4_5321 at 70.824 kb on - strand, within Pf1N1B4_5321 at 71.058 kb on + strand at 71.058 kb on + strand at 71.058 kb on + strand at 71.058 kb on + strand at 71.058 kb on + strand at 71.058 kb on + strand at 71.059 kb on - strand at 71.059 kb on - strand at 71.059 kb on - strand at 71.092 kb on + strand at 71.092 kb on + strand at 71.093 kb on - strand at 71.114 kb on + strand at 71.114 kb on + strand at 71.115 kb on - strand at 71.131 kb on - strand at 71.139 kb on - strand at 71.229 kb on + strand at 71.335 kb on + strand at 71.335 kb on + strand at 71.336 kb on - strand at 71.356 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.433 kb on + strand at 71.437 kb on + strand at 71.437 kb on + strand at 71.437 kb on + strand at 71.481 kb on - strand at 71.499 kb on + strand at 71.669 kb on + strand at 71.669 kb on + strand at 71.669 kb on + strand at 71.669 kb on + strand at 71.670 kb on - strand at 71.670 kb on - strand at 71.715 kb on - strand at 71.752 kb on + strand, within Pf1N1B4_5322 at 71.753 kb on - strand, within Pf1N1B4_5322 at 71.763 kb on - strand, within Pf1N1B4_5322 at 71.880 kb on + strand, within Pf1N1B4_5322 at 71.880 kb on + strand, within Pf1N1B4_5322 at 71.898 kb on + strand at 71.899 kb on - strand at 71.913 kb on + strand at 71.917 kb on + strand at 71.917 kb on + strand at 71.918 kb on - strand at 71.926 kb on + strand at 71.926 kb on + strand at 71.927 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 69,644 - Pf1N1B4_5320 0.78 +1.8 69,700 + +1.2 69,701 - -1.9 69,701 - +3.0 69,748 - -1.6 69,748 - +1.2 69,748 - -1.4 69,871 + +1.4 69,871 + +1.2 69,872 - +0.2 69,872 - +1.4 69,872 - +1.2 69,931 - -2.4 70,018 + -1.3 70,018 + +3.2 70,018 + +1.4 70,018 + -0.8 70,019 - -1.6 70,019 - -1.2 70,019 - +1.2 70,019 - +1.2 70,019 - +0.2 70,025 + -0.3 70,026 - +0.7 70,026 - -0.1 70,027 + -0.2 70,046 - -1.8 70,046 - +1.2 70,079 + +0.7 70,079 + -0.2 70,102 - +0.2 70,102 - -0.8 70,104 + -1.4 70,104 + +2.2 70,126 + -0.8 70,126 + +0.6 70,126 + -0.0 70,127 - +1.9 70,136 + -0.4 70,136 + +0.6 70,141 + -1.2 70,141 + +1.3 70,141 + -0.2 70,141 + +1.7 70,141 + -1.4 70,151 - +2.2 70,156 + +0.6 70,156 + -0.1 70,156 + +0.8 70,157 - -2.2 70,314 - +1.0 70,403 + -0.1 70,403 + +1.2 70,403 + -1.2 70,404 - +1.2 70,422 + -0.1 70,422 + +0.6 70,422 + +0.2 70,455 + -0.1 70,470 + -1.4 70,473 + -0.6 70,474 - -0.4 70,474 - -1.4 70,474 - -0.4 70,474 - +2.2 70,477 + -2.2 70,478 - +1.2 70,519 + +0.6 70,519 + -0.1 70,520 - +0.4 70,552 + +0.8 70,595 + +0.8 70,596 - +1.2 70,617 + -0.6 70,617 + -1.8 70,621 + +1.4 70,621 + +0.3 70,622 - -0.1 70,622 - +0.6 70,622 - +0.8 70,640 + +0.6 70,640 + +0.6 70,641 - +2.2 70,684 - Pf1N1B4_5321 0.13 -0.4 70,698 + Pf1N1B4_5321 0.17 -1.8 70,699 - Pf1N1B4_5321 0.18 -0.4 70,699 - Pf1N1B4_5321 0.18 -0.8 70,699 - Pf1N1B4_5321 0.18 +0.2 70,709 + Pf1N1B4_5321 0.21 -2.4 70,709 + +1.2 70,709 + Pf1N1B4_5321 0.21 -1.6 70,709 + Pf1N1B4_5321 0.21 -1.2 70,709 + Pf1N1B4_5321 0.21 -0.2 70,710 - +1.2 70,710 - Pf1N1B4_5321 0.21 +0.2 70,722 - Pf1N1B4_5321 0.25 -1.4 70,722 - Pf1N1B4_5321 0.25 -1.2 70,722 - Pf1N1B4_5321 0.25 +0.6 70,824 - Pf1N1B4_5321 0.55 -0.0 71,058 + -0.0 71,058 + -0.0 71,058 + +0.2 71,058 + -1.6 71,058 + +1.2 71,058 + -2.2 71,059 - -1.8 71,059 - -1.0 71,059 - +0.6 71,092 + +0.8 71,092 + +1.0 71,093 - -1.6 71,114 + +0.5 71,114 + +0.2 71,115 - +0.6 71,131 - +0.2 71,139 - +0.7 71,229 + +0.8 71,335 + -0.1 71,335 + -0.2 71,336 - +0.6 71,356 + -0.8 71,358 + -1.4 71,358 + -0.8 71,358 + -0.8 71,358 + -0.1 71,358 + -1.8 71,358 + +1.2 71,358 + +0.2 71,359 - +0.7 71,359 - -0.8 71,359 - +0.4 71,359 - +0.2 71,359 - +0.6 71,359 - -0.8 71,433 + -0.0 71,437 + -0.6 71,437 + +0.2 71,437 + -0.1 71,481 - +0.8 71,499 + -0.6 71,669 + -0.6 71,669 + -1.6 71,669 + +0.5 71,669 + -0.8 71,670 - -0.4 71,670 - -0.8 71,715 - +2.2 71,752 + Pf1N1B4_5322 0.20 -0.3 71,753 - Pf1N1B4_5322 0.20 -0.2 71,763 - Pf1N1B4_5322 0.26 -0.5 71,880 + Pf1N1B4_5322 0.89 -0.8 71,880 + Pf1N1B4_5322 0.89 +0.4 71,898 + +2.4 71,899 - +1.8 71,913 + -0.8 71,917 + +1.2 71,917 + +0.9 71,918 - -1.0 71,926 + +0.8 71,926 + -0.4 71,927 - -0.0
Or see this region's nucleotide sequence