Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5251 and Pf1N1B4_5252 are separated by 169 nucleotides Pf1N1B4_5252 and Pf1N1B4_5253 are separated by 11 nucleotides
Pf1N1B4_5251 - hypothetical protein, at 9,517 to 10,368
_5251
Pf1N1B4_5252 - hypothetical protein, at 10,538 to 11,272
_5252
Pf1N1B4_5253 - hypothetical protein, at 11,284 to 12,045
_5253
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 9.558 kb on + strand at 9.558 kb on + strand at 9.596 kb on + strand at 9.597 kb on - strand at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.712 kb on + strand, within Pf1N1B4_5251 at 9.920 kb on + strand at 9.920 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.137 kb on - strand, within Pf1N1B4_5251 at 10.137 kb on - strand, within Pf1N1B4_5251 at 10.137 kb on - strand at 10.137 kb on - strand at 10.357 kb on - strand at 10.384 kb on + strand at 10.401 kb on - strand at 10.401 kb on - strand at 10.453 kb on - strand at 10.453 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.552 kb on - strand at 10.733 kb on + strand, within Pf1N1B4_5252 at 10.780 kb on - strand, within Pf1N1B4_5252 at 10.803 kb on + strand, within Pf1N1B4_5252 at 10.803 kb on + strand, within Pf1N1B4_5252 at 10.819 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.841 kb on - strand, within Pf1N1B4_5252 at 10.929 kb on + strand, within Pf1N1B4_5252 at 11.130 kb on + strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.134 kb on + strand, within Pf1N1B4_5252 at 11.134 kb on + strand, within Pf1N1B4_5252 at 11.159 kb on + strand, within Pf1N1B4_5252 at 11.205 kb on + strand at 11.206 kb on - strand at 11.206 kb on - strand at 11.206 kb on - strand at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.409 kb on - strand, within Pf1N1B4_5253 at 11.423 kb on + strand, within Pf1N1B4_5253 at 11.423 kb on + strand, within Pf1N1B4_5253 at 11.424 kb on - strand, within Pf1N1B4_5253 at 11.432 kb on + strand, within Pf1N1B4_5253 at 11.432 kb on + strand at 11.433 kb on - strand, within Pf1N1B4_5253 at 11.790 kb on - strand at 11.790 kb on - strand, within Pf1N1B4_5253 at 11.864 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.921 kb on - strand, within Pf1N1B4_5253 at 11.921 kb on - strand, within Pf1N1B4_5253 at 11.924 kb on + strand, within Pf1N1B4_5253 at 11.924 kb on + strand, within Pf1N1B4_5253 at 11.924 kb on + strand, within Pf1N1B4_5253 at 11.925 kb on - strand, within Pf1N1B4_5253 at 11.925 kb on - strand, within Pf1N1B4_5253 at 11.969 kb on + strand at 11.969 kb on + strand at 12.033 kb on + strand at 12.033 kb on + strand at 12.033 kb on + strand at 12.033 kb on + strand at 12.034 kb on - strand at 12.034 kb on - strand at 12.034 kb on - strand at 12.068 kb on - strand at 12.068 kb on - strand at 12.153 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 9,558 + +1.2 9,558 + +0.2 9,596 + +0.1 9,597 - +0.6 9,640 - Pf1N1B4_5251 0.14 -0.6 9,640 - Pf1N1B4_5251 0.14 +0.2 9,640 - Pf1N1B4_5251 0.14 +0.4 9,640 - Pf1N1B4_5251 0.14 -1.2 9,640 - Pf1N1B4_5251 0.14 +0.5 9,640 - Pf1N1B4_5251 0.14 -0.9 9,712 + Pf1N1B4_5251 0.23 -0.6 9,920 + +0.6 9,920 + Pf1N1B4_5251 0.47 -0.8 10,136 + Pf1N1B4_5251 0.73 -1.2 10,136 + Pf1N1B4_5251 0.73 +0.3 10,136 + Pf1N1B4_5251 0.73 -0.4 10,136 + Pf1N1B4_5251 0.73 +0.4 10,136 + Pf1N1B4_5251 0.73 -0.7 10,136 + Pf1N1B4_5251 0.73 +1.9 10,136 + Pf1N1B4_5251 0.73 -0.2 10,136 + Pf1N1B4_5251 0.73 -0.8 10,137 - Pf1N1B4_5251 0.73 +0.8 10,137 - Pf1N1B4_5251 0.73 -0.7 10,137 - -0.4 10,137 - +1.2 10,357 - -0.0 10,384 + +0.3 10,401 - +0.4 10,401 - +1.8 10,453 - +0.8 10,453 - +1.0 10,496 - +0.1 10,496 - +1.8 10,496 - +0.6 10,496 - -1.2 10,500 - +1.2 10,500 - -0.2 10,500 - -1.6 10,500 - +0.3 10,552 - +0.2 10,733 + Pf1N1B4_5252 0.27 -0.7 10,780 - Pf1N1B4_5252 0.33 +1.1 10,803 + Pf1N1B4_5252 0.36 -1.8 10,803 + Pf1N1B4_5252 0.36 +1.8 10,819 + Pf1N1B4_5252 0.38 -0.1 10,821 + Pf1N1B4_5252 0.39 +0.2 10,821 + Pf1N1B4_5252 0.39 -0.1 10,821 + Pf1N1B4_5252 0.39 -0.2 10,822 - Pf1N1B4_5252 0.39 +2.9 10,822 - Pf1N1B4_5252 0.39 +1.1 10,822 - Pf1N1B4_5252 0.39 -0.0 10,841 - Pf1N1B4_5252 0.41 -0.8 10,929 + Pf1N1B4_5252 0.53 +1.9 11,130 + Pf1N1B4_5252 0.81 -1.2 11,131 - Pf1N1B4_5252 0.81 +1.0 11,131 - Pf1N1B4_5252 0.81 +0.9 11,131 - Pf1N1B4_5252 0.81 -0.8 11,134 + Pf1N1B4_5252 0.81 -1.4 11,134 + Pf1N1B4_5252 0.81 +1.2 11,159 + Pf1N1B4_5252 0.84 -0.1 11,205 + -0.2 11,206 - -0.8 11,206 - +0.3 11,206 - -1.2 11,408 + Pf1N1B4_5253 0.16 -0.4 11,408 + Pf1N1B4_5253 0.16 +0.3 11,408 + Pf1N1B4_5253 0.16 +0.2 11,408 + Pf1N1B4_5253 0.16 +0.6 11,409 - Pf1N1B4_5253 0.16 +1.9 11,423 + Pf1N1B4_5253 0.18 -2.2 11,423 + Pf1N1B4_5253 0.18 +0.8 11,424 - Pf1N1B4_5253 0.18 +2.6 11,432 + Pf1N1B4_5253 0.19 -1.0 11,432 + -0.8 11,433 - Pf1N1B4_5253 0.20 -0.4 11,790 - -0.2 11,790 - Pf1N1B4_5253 0.66 +0.6 11,864 + Pf1N1B4_5253 0.76 +1.2 11,920 + Pf1N1B4_5253 0.83 +0.4 11,920 + Pf1N1B4_5253 0.83 +2.8 11,920 + Pf1N1B4_5253 0.83 -0.8 11,920 + Pf1N1B4_5253 0.83 +0.2 11,921 - Pf1N1B4_5253 0.84 -0.0 11,921 - Pf1N1B4_5253 0.84 +1.0 11,924 + Pf1N1B4_5253 0.84 -0.1 11,924 + Pf1N1B4_5253 0.84 +1.6 11,924 + Pf1N1B4_5253 0.84 -0.3 11,925 - Pf1N1B4_5253 0.84 +1.8 11,925 - Pf1N1B4_5253 0.84 +0.4 11,969 + +1.2 11,969 + +1.7 12,033 + -0.2 12,033 + -1.6 12,033 + +0.6 12,033 + +1.8 12,034 - +1.0 12,034 - +0.6 12,034 - +0.8 12,068 - +0.8 12,068 - -1.4 12,153 + -1.2
Or see this region's nucleotide sequence