Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5074

Experiment: Choline Lysine 6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5072 and Pf1N1B4_5073 are separated by 4 nucleotidesPf1N1B4_5073 and Pf1N1B4_5074 are separated by 115 nucleotidesPf1N1B4_5074 and Pf1N1B4_5075 are separated by 232 nucleotidesPf1N1B4_5075 and Pf1N1B4_5076 overlap by 4 nucleotides Pf1N1B4_5072 - Glycerol-3-phosphate regulon repressor, at 5,617,764 to 5,618,582 _5072 Pf1N1B4_5073 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 5,618,587 to 5,619,369 _5073 Pf1N1B4_5074 - Gluconate permease, at 5,619,485 to 5,620,897 _5074 Pf1N1B4_5075 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 5,621,130 to 5,621,768 _5075 Pf1N1B4_5076 - FIG00641944: hypothetical protein, at 5,621,765 to 5,623,057 _5076 Position (kb) 5619 5620 5621Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5618.580 kb on + strandat 5618.581 kb on - strandat 5618.635 kb on - strandat 5618.673 kb on - strand, within Pf1N1B4_5073at 5618.709 kb on - strand, within Pf1N1B4_5073at 5618.709 kb on - strandat 5618.764 kb on - strand, within Pf1N1B4_5073at 5619.005 kb on + strandat 5619.092 kb on + strand, within Pf1N1B4_5073at 5619.093 kb on - strandat 5619.117 kb on + strand, within Pf1N1B4_5073at 5619.269 kb on + strand, within Pf1N1B4_5073at 5619.379 kb on + strandat 5619.379 kb on + strandat 5619.380 kb on - strandat 5619.380 kb on - strandat 5619.380 kb on - strandat 5619.380 kb on - strandat 5619.380 kb on - strandat 5619.380 kb on - strandat 5619.380 kb on - strandat 5619.380 kb on - strandat 5619.383 kb on + strandat 5619.383 kb on + strandat 5619.383 kb on + strandat 5619.384 kb on - strandat 5619.384 kb on - strandat 5619.455 kb on + strandat 5619.511 kb on + strandat 5619.512 kb on - strandat 5619.955 kb on + strandat 5620.299 kb on + strand, within Pf1N1B4_5074at 5620.300 kb on - strandat 5620.374 kb on + strand, within Pf1N1B4_5074at 5620.375 kb on - strand, within Pf1N1B4_5074at 5620.375 kb on - strand, within Pf1N1B4_5074at 5620.534 kb on + strand, within Pf1N1B4_5074at 5620.875 kb on + strandat 5621.108 kb on - strandat 5621.524 kb on + strand, within Pf1N1B4_5075

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 6 mM
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5,618,580 + -0.4
5,618,581 - -1.7
5,618,635 - +2.3
5,618,673 - Pf1N1B4_5073 0.11 -0.7
5,618,709 - Pf1N1B4_5073 0.16 -0.9
5,618,709 - +1.9
5,618,764 - Pf1N1B4_5073 0.23 -1.0
5,619,005 + +0.3
5,619,092 + Pf1N1B4_5073 0.64 -0.8
5,619,093 - +2.3
5,619,117 + Pf1N1B4_5073 0.68 +0.6
5,619,269 + Pf1N1B4_5073 0.87 +0.3
5,619,379 + -1.0
5,619,379 + -2.0
5,619,380 - +2.9
5,619,380 - -0.1
5,619,380 - -0.7
5,619,380 - +0.3
5,619,380 - +0.3
5,619,380 - -2.0
5,619,380 - +1.9
5,619,380 - +0.9
5,619,383 + -0.4
5,619,383 + +0.7
5,619,383 + -0.7
5,619,384 - -0.3
5,619,384 - +0.3
5,619,455 + +1.5
5,619,511 + +1.6
5,619,512 - +0.3
5,619,955 + -0.0
5,620,299 + Pf1N1B4_5074 0.58 -2.7
5,620,300 - -1.7
5,620,374 + Pf1N1B4_5074 0.63 -2.5
5,620,375 - Pf1N1B4_5074 0.63 -2.3
5,620,375 - Pf1N1B4_5074 0.63 -0.1
5,620,534 + Pf1N1B4_5074 0.74 +0.8
5,620,875 + +1.3
5,621,108 - -2.0
5,621,524 + Pf1N1B4_5075 0.62 +0.6

Or see this region's nucleotide sequence