Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4873 and Pf1N1B4_4874 are separated by 258 nucleotides Pf1N1B4_4874 and Pf1N1B4_4875 are separated by 274 nucleotides
Pf1N1B4_4873 - clavaldehyde dehydrogenase, at 5,392,856 to 5,393,617
_4873
Pf1N1B4_4874 - hypothetical protein, at 5,393,876 to 5,394,127
_4874
Pf1N1B4_4875 - Msl4750 protein, at 5,394,402 to 5,394,779
_4875
Position (kb) 5393
5394
5395 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5393.002 kb on - strand, within Pf1N1B4_4873 at 5393.153 kb on - strand, within Pf1N1B4_4873 at 5393.216 kb on + strand at 5393.216 kb on + strand at 5393.216 kb on + strand, within Pf1N1B4_4873 at 5393.217 kb on - strand, within Pf1N1B4_4873 at 5393.316 kb on + strand, within Pf1N1B4_4873 at 5393.330 kb on + strand at 5393.331 kb on - strand, within Pf1N1B4_4873 at 5393.331 kb on - strand, within Pf1N1B4_4873 at 5393.340 kb on - strand, within Pf1N1B4_4873 at 5393.498 kb on + strand, within Pf1N1B4_4873 at 5393.550 kb on + strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.616 kb on - strand at 5393.679 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.794 kb on - strand at 5393.900 kb on - strand at 5393.927 kb on - strand, within Pf1N1B4_4874 at 5394.013 kb on + strand, within Pf1N1B4_4874 at 5394.060 kb on + strand, within Pf1N1B4_4874 at 5394.060 kb on + strand, within Pf1N1B4_4874 at 5394.125 kb on + strand at 5394.267 kb on + strand at 5394.311 kb on + strand at 5394.311 kb on + strand at 5394.311 kb on + strand at 5394.311 kb on + strand at 5394.312 kb on - strand at 5394.312 kb on - strand at 5394.312 kb on - strand at 5394.312 kb on - strand at 5394.376 kb on + strand at 5394.398 kb on + strand at 5394.398 kb on + strand at 5394.398 kb on + strand at 5394.399 kb on - strand at 5394.399 kb on - strand at 5394.399 kb on - strand at 5394.489 kb on + strand, within Pf1N1B4_4875 at 5394.489 kb on + strand, within Pf1N1B4_4875 at 5394.490 kb on - strand, within Pf1N1B4_4875 at 5394.504 kb on + strand, within Pf1N1B4_4875 at 5394.505 kb on - strand, within Pf1N1B4_4875 at 5394.537 kb on + strand at 5394.537 kb on + strand, within Pf1N1B4_4875 at 5394.537 kb on + strand, within Pf1N1B4_4875 at 5394.537 kb on + strand, within Pf1N1B4_4875 at 5394.538 kb on - strand, within Pf1N1B4_4875 at 5394.570 kb on + strand, within Pf1N1B4_4875 at 5394.570 kb on + strand, within Pf1N1B4_4875 at 5394.571 kb on - strand, within Pf1N1B4_4875 at 5394.681 kb on + strand, within Pf1N1B4_4875 at 5394.697 kb on - strand, within Pf1N1B4_4875 at 5394.697 kb on - strand, within Pf1N1B4_4875 at 5394.759 kb on + strand at 5394.759 kb on + strand at 5394.759 kb on + strand at 5394.827 kb on + strand at 5394.875 kb on + strand at 5394.875 kb on + strand at 5394.875 kb on + strand at 5394.875 kb on + strand at 5394.876 kb on - strand at 5394.876 kb on - strand at 5394.876 kb on - strand at 5394.930 kb on - strand at 5395.049 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 5,393,002 - Pf1N1B4_4873 0.19 -0.9 5,393,153 - Pf1N1B4_4873 0.39 -2.5 5,393,216 + +0.3 5,393,216 + +0.3 5,393,216 + Pf1N1B4_4873 0.47 +0.3 5,393,217 - Pf1N1B4_4873 0.47 -1.3 5,393,316 + Pf1N1B4_4873 0.60 -2.3 5,393,330 + -0.7 5,393,331 - Pf1N1B4_4873 0.62 -1.3 5,393,331 - Pf1N1B4_4873 0.62 -2.0 5,393,340 - Pf1N1B4_4873 0.64 -0.6 5,393,498 + Pf1N1B4_4873 0.84 -0.7 5,393,550 + -1.7 5,393,550 - +1.0 5,393,550 - -1.3 5,393,550 - -1.5 5,393,550 - -0.7 5,393,616 - -0.1 5,393,679 + -0.7 5,393,694 + +1.6 5,393,694 + -0.4 5,393,694 + -0.9 5,393,694 + +0.4 5,393,694 + +0.3 5,393,695 - -0.6 5,393,695 - -0.1 5,393,695 - -1.3 5,393,695 - +0.3 5,393,695 - -0.9 5,393,695 - -1.3 5,393,794 - -0.5 5,393,900 - +0.3 5,393,927 - Pf1N1B4_4874 0.20 -0.0 5,394,013 + Pf1N1B4_4874 0.54 -2.0 5,394,060 + Pf1N1B4_4874 0.73 -1.3 5,394,060 + Pf1N1B4_4874 0.73 -2.7 5,394,125 + +0.3 5,394,267 + -1.7 5,394,311 + -0.3 5,394,311 + +1.9 5,394,311 + -2.0 5,394,311 + -0.7 5,394,312 - -0.9 5,394,312 - +1.3 5,394,312 - -0.7 5,394,312 - -1.7 5,394,376 + +2.3 5,394,398 + -1.1 5,394,398 + +0.2 5,394,398 + -1.3 5,394,399 - -0.9 5,394,399 - +0.6 5,394,399 - -0.1 5,394,489 + Pf1N1B4_4875 0.23 -0.2 5,394,489 + Pf1N1B4_4875 0.23 -0.9 5,394,490 - Pf1N1B4_4875 0.23 +0.7 5,394,504 + Pf1N1B4_4875 0.27 +0.2 5,394,505 - Pf1N1B4_4875 0.27 +0.4 5,394,537 + +1.3 5,394,537 + Pf1N1B4_4875 0.36 -2.7 5,394,537 + Pf1N1B4_4875 0.36 -0.3 5,394,537 + Pf1N1B4_4875 0.36 +0.3 5,394,538 - Pf1N1B4_4875 0.36 -0.1 5,394,570 + Pf1N1B4_4875 0.44 -0.0 5,394,570 + Pf1N1B4_4875 0.44 -1.7 5,394,571 - Pf1N1B4_4875 0.45 +0.9 5,394,681 + Pf1N1B4_4875 0.74 -1.0 5,394,697 - Pf1N1B4_4875 0.78 -0.6 5,394,697 - Pf1N1B4_4875 0.78 -0.1 5,394,759 + -1.3 5,394,759 + +0.3 5,394,759 + -1.1 5,394,827 + +0.7 5,394,875 + +0.7 5,394,875 + -0.7 5,394,875 + -0.0 5,394,875 + -1.3 5,394,876 - -0.1 5,394,876 - +0.0 5,394,876 - -0.4 5,394,930 - -0.9 5,395,049 - -0.3
Or see this region's nucleotide sequence