Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4869 and Pf1N1B4_4870 are separated by 12 nucleotides Pf1N1B4_4870 and Pf1N1B4_4871 are separated by 109 nucleotides Pf1N1B4_4871 and Pf1N1B4_4872 are separated by 80 nucleotides Pf1N1B4_4872 and Pf1N1B4_4873 are separated by 37 nucleotides
Pf1N1B4_4869 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 5,388,805 to 5,391,045
_4869
Pf1N1B4_4870 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 5,391,058 to 5,391,531
_4870
Pf1N1B4_4871 - Aldo-keto reductase, at 5,391,641 to 5,391,895
_4871
Pf1N1B4_4872 - Short-chain dehydrogenase/reductase SDR, at 5,391,976 to 5,392,818
_4872
Pf1N1B4_4873 - clavaldehyde dehydrogenase, at 5,392,856 to 5,393,617
_4873
Position (kb)
5391
5392
5393 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5390.995 kb on + strand at 5390.995 kb on + strand at 5390.995 kb on + strand at 5390.996 kb on - strand at 5390.996 kb on - strand at 5391.271 kb on + strand, within Pf1N1B4_4870 at 5391.271 kb on + strand at 5391.393 kb on + strand, within Pf1N1B4_4870 at 5391.394 kb on - strand, within Pf1N1B4_4870 at 5391.394 kb on - strand, within Pf1N1B4_4870 at 5391.487 kb on + strand at 5391.500 kb on + strand at 5391.501 kb on - strand at 5391.596 kb on + strand at 5391.597 kb on - strand at 5391.727 kb on + strand, within Pf1N1B4_4871 at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.948 kb on - strand at 5391.951 kb on + strand at 5392.028 kb on + strand at 5392.028 kb on + strand at 5392.490 kb on + strand, within Pf1N1B4_4872 at 5392.555 kb on + strand, within Pf1N1B4_4872 at 5392.556 kb on - strand, within Pf1N1B4_4872 at 5392.579 kb on + strand, within Pf1N1B4_4872 at 5392.580 kb on - strand, within Pf1N1B4_4872 at 5392.782 kb on + strand at 5392.782 kb on + strand at 5393.002 kb on - strand, within Pf1N1B4_4873 at 5393.153 kb on - strand, within Pf1N1B4_4873 at 5393.216 kb on + strand at 5393.216 kb on + strand at 5393.216 kb on + strand, within Pf1N1B4_4873 at 5393.217 kb on - strand, within Pf1N1B4_4873 at 5393.316 kb on + strand, within Pf1N1B4_4873 at 5393.330 kb on + strand at 5393.331 kb on - strand, within Pf1N1B4_4873 at 5393.331 kb on - strand, within Pf1N1B4_4873 at 5393.340 kb on - strand, within Pf1N1B4_4873 at 5393.498 kb on + strand, within Pf1N1B4_4873 at 5393.550 kb on + strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.616 kb on - strand at 5393.679 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.794 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 5,390,995 + +1.2 5,390,995 + -1.4 5,390,995 + -0.3 5,390,996 - +0.0 5,390,996 - +0.3 5,391,271 + Pf1N1B4_4870 0.45 +0.8 5,391,271 + -0.7 5,391,393 + Pf1N1B4_4870 0.71 -0.3 5,391,394 - Pf1N1B4_4870 0.71 +0.9 5,391,394 - Pf1N1B4_4870 0.71 +0.3 5,391,487 + +2.2 5,391,500 + -1.6 5,391,501 - -0.3 5,391,596 + +0.3 5,391,597 - +0.8 5,391,727 + Pf1N1B4_4871 0.34 -0.1 5,391,947 + -0.8 5,391,947 + -1.3 5,391,947 + -1.5 5,391,947 + -0.7 5,391,947 + -0.1 5,391,948 - -0.7 5,391,951 + -2.0 5,392,028 + -0.7 5,392,028 + -0.7 5,392,490 + Pf1N1B4_4872 0.61 -0.4 5,392,555 + Pf1N1B4_4872 0.69 +0.3 5,392,556 - Pf1N1B4_4872 0.69 -0.1 5,392,579 + Pf1N1B4_4872 0.72 +0.2 5,392,580 - Pf1N1B4_4872 0.72 +0.3 5,392,782 + +0.1 5,392,782 + +1.5 5,393,002 - Pf1N1B4_4873 0.19 -0.9 5,393,153 - Pf1N1B4_4873 0.39 -2.5 5,393,216 + +0.3 5,393,216 + +0.3 5,393,216 + Pf1N1B4_4873 0.47 +0.3 5,393,217 - Pf1N1B4_4873 0.47 -1.3 5,393,316 + Pf1N1B4_4873 0.60 -2.3 5,393,330 + -0.7 5,393,331 - Pf1N1B4_4873 0.62 -1.3 5,393,331 - Pf1N1B4_4873 0.62 -2.0 5,393,340 - Pf1N1B4_4873 0.64 -0.6 5,393,498 + Pf1N1B4_4873 0.84 -0.7 5,393,550 + -1.7 5,393,550 - +1.0 5,393,550 - -1.3 5,393,550 - -1.5 5,393,550 - -0.7 5,393,616 - -0.1 5,393,679 + -0.7 5,393,694 + +1.6 5,393,694 + -0.4 5,393,694 + -0.9 5,393,694 + +0.4 5,393,694 + +0.3 5,393,695 - -0.6 5,393,695 - -0.1 5,393,695 - -1.3 5,393,695 - +0.3 5,393,695 - -0.9 5,393,695 - -1.3 5,393,794 - -0.5
Or see this region's nucleotide sequence