Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4863 and Pf1N1B4_4864 overlap by 4 nucleotides Pf1N1B4_4864 and Pf1N1B4_4865 are separated by 12 nucleotides
Pf1N1B4_4863 - Outer membrane protein OprN, at 5,378,345 to 5,379,766
_4863
Pf1N1B4_4864 - Multidrug efflux transporter MexF, at 5,379,763 to 5,382,954
_4864
Pf1N1B4_4865 - Multidrug efflux membrane fusion protein MexE, at 5,382,967 to 5,384,220
_4865
Position (kb)
5379
5380
5381
5382
5383 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5379.232 kb on - strand, within Pf1N1B4_4863 at 5379.379 kb on - strand, within Pf1N1B4_4863 at 5379.851 kb on - strand at 5379.851 kb on - strand at 5379.854 kb on + strand at 5379.854 kb on + strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5380.110 kb on + strand, within Pf1N1B4_4864 at 5380.111 kb on - strand, within Pf1N1B4_4864 at 5380.111 kb on - strand at 5380.254 kb on - strand, within Pf1N1B4_4864 at 5380.286 kb on - strand, within Pf1N1B4_4864 at 5380.337 kb on + strand at 5380.338 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.625 kb on + strand, within Pf1N1B4_4864 at 5380.625 kb on + strand, within Pf1N1B4_4864 at 5380.658 kb on + strand, within Pf1N1B4_4864 at 5380.673 kb on + strand, within Pf1N1B4_4864 at 5380.772 kb on + strand, within Pf1N1B4_4864 at 5380.773 kb on - strand, within Pf1N1B4_4864 at 5380.773 kb on - strand, within Pf1N1B4_4864 at 5380.841 kb on + strand, within Pf1N1B4_4864 at 5380.842 kb on - strand, within Pf1N1B4_4864 at 5380.943 kb on + strand, within Pf1N1B4_4864 at 5380.943 kb on + strand, within Pf1N1B4_4864 at 5380.944 kb on - strand, within Pf1N1B4_4864 at 5380.944 kb on - strand, within Pf1N1B4_4864 at 5381.149 kb on - strand, within Pf1N1B4_4864 at 5381.149 kb on - strand, within Pf1N1B4_4864 at 5381.149 kb on - strand, within Pf1N1B4_4864 at 5381.217 kb on - strand, within Pf1N1B4_4864 at 5381.336 kb on - strand, within Pf1N1B4_4864 at 5381.349 kb on - strand, within Pf1N1B4_4864 at 5381.349 kb on - strand, within Pf1N1B4_4864 at 5381.349 kb on - strand at 5381.548 kb on - strand, within Pf1N1B4_4864 at 5381.549 kb on + strand at 5381.890 kb on + strand, within Pf1N1B4_4864 at 5381.966 kb on + strand, within Pf1N1B4_4864 at 5381.966 kb on + strand, within Pf1N1B4_4864 at 5382.196 kb on - strand, within Pf1N1B4_4864 at 5382.479 kb on + strand, within Pf1N1B4_4864 at 5382.480 kb on - strand at 5382.480 kb on - strand, within Pf1N1B4_4864 at 5382.491 kb on + strand, within Pf1N1B4_4864 at 5382.492 kb on - strand, within Pf1N1B4_4864 at 5382.719 kb on + strand at 5382.719 kb on + strand at 5382.719 kb on + strand at 5382.719 kb on + strand at 5382.719 kb on + strand at 5382.720 kb on - strand at 5382.720 kb on - strand at 5382.957 kb on + strand at 5382.957 kb on + strand at 5383.331 kb on + strand, within Pf1N1B4_4865 at 5383.554 kb on - strand, within Pf1N1B4_4865 at 5383.557 kb on + strand, within Pf1N1B4_4865 at 5383.558 kb on - strand, within Pf1N1B4_4865 at 5383.558 kb on - strand, within Pf1N1B4_4865 at 5383.689 kb on + strand, within Pf1N1B4_4865
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 5,379,232 - Pf1N1B4_4863 0.62 +1.9 5,379,379 - Pf1N1B4_4863 0.73 -0.1 5,379,851 - -0.4 5,379,851 - -0.4 5,379,854 + -0.7 5,379,854 + +1.3 5,379,855 - +0.9 5,379,855 - +0.3 5,379,855 - -0.5 5,379,855 - -0.1 5,379,855 - -1.1 5,379,855 - +0.5 5,379,855 - +0.4 5,380,110 + Pf1N1B4_4864 0.11 -0.1 5,380,111 - Pf1N1B4_4864 0.11 +1.1 5,380,111 - +1.9 5,380,254 - Pf1N1B4_4864 0.15 +2.1 5,380,286 - Pf1N1B4_4864 0.16 -0.1 5,380,337 + -0.7 5,380,338 - Pf1N1B4_4864 0.18 -0.2 5,380,476 - Pf1N1B4_4864 0.22 +0.8 5,380,476 - Pf1N1B4_4864 0.22 +1.2 5,380,476 - Pf1N1B4_4864 0.22 +0.7 5,380,476 - Pf1N1B4_4864 0.22 -0.8 5,380,625 + Pf1N1B4_4864 0.27 -2.0 5,380,625 + Pf1N1B4_4864 0.27 -2.0 5,380,658 + Pf1N1B4_4864 0.28 +0.7 5,380,673 + Pf1N1B4_4864 0.29 +1.3 5,380,772 + Pf1N1B4_4864 0.32 -0.2 5,380,773 - Pf1N1B4_4864 0.32 +0.2 5,380,773 - Pf1N1B4_4864 0.32 +1.0 5,380,841 + Pf1N1B4_4864 0.34 -0.4 5,380,842 - Pf1N1B4_4864 0.34 -2.7 5,380,943 + Pf1N1B4_4864 0.37 -0.0 5,380,943 + Pf1N1B4_4864 0.37 -2.7 5,380,944 - Pf1N1B4_4864 0.37 +0.7 5,380,944 - Pf1N1B4_4864 0.37 +0.3 5,381,149 - Pf1N1B4_4864 0.43 -1.0 5,381,149 - Pf1N1B4_4864 0.43 -1.3 5,381,149 - Pf1N1B4_4864 0.43 +0.3 5,381,217 - Pf1N1B4_4864 0.46 -1.3 5,381,336 - Pf1N1B4_4864 0.49 -0.4 5,381,349 - Pf1N1B4_4864 0.50 -2.0 5,381,349 - Pf1N1B4_4864 0.50 -0.1 5,381,349 - -0.3 5,381,548 - Pf1N1B4_4864 0.56 +0.4 5,381,549 + -0.7 5,381,890 + Pf1N1B4_4864 0.67 -0.8 5,381,966 + Pf1N1B4_4864 0.69 -2.3 5,381,966 + Pf1N1B4_4864 0.69 -0.7 5,382,196 - Pf1N1B4_4864 0.76 +1.1 5,382,479 + Pf1N1B4_4864 0.85 -0.2 5,382,480 - +0.3 5,382,480 - Pf1N1B4_4864 0.85 -0.0 5,382,491 + Pf1N1B4_4864 0.85 +0.3 5,382,492 - Pf1N1B4_4864 0.85 -1.3 5,382,719 + -1.3 5,382,719 + -0.3 5,382,719 + +0.3 5,382,719 + -0.7 5,382,719 + -2.3 5,382,720 - -1.7 5,382,720 - -1.7 5,382,957 + +0.7 5,382,957 + -0.1 5,383,331 + Pf1N1B4_4865 0.29 -0.4 5,383,554 - Pf1N1B4_4865 0.47 -0.1 5,383,557 + Pf1N1B4_4865 0.47 -0.3 5,383,558 - Pf1N1B4_4865 0.47 +0.1 5,383,558 - Pf1N1B4_4865 0.47 +1.1 5,383,689 + Pf1N1B4_4865 0.58 -0.6
Or see this region's nucleotide sequence