Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4386 and Pf1N1B4_4387 are separated by 162 nucleotides Pf1N1B4_4387 and Pf1N1B4_4388 overlap by 4 nucleotides Pf1N1B4_4388 and Pf1N1B4_4389 are separated by 200 nucleotides
Pf1N1B4_4386 - Inositol transport system sugar-binding protein, at 4,844,516 to 4,845,442
_4386
Pf1N1B4_4387 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 4,845,605 to 4,846,393
_4387
Pf1N1B4_4388 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 4,846,390 to 4,847,412
_4388
Pf1N1B4_4389 - Sigma-54 dependent transcriptional regulator, at 4,847,613 to 4,849,634
_4389
Position (kb)
4846
4847
4848 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4845.408 kb on - strand at 4845.463 kb on + strand at 4845.510 kb on + strand at 4845.511 kb on - strand at 4845.541 kb on - strand at 4845.570 kb on + strand at 4845.571 kb on - strand at 4845.571 kb on - strand at 4845.956 kb on - strand, within Pf1N1B4_4387 at 4846.547 kb on + strand, within Pf1N1B4_4388 at 4846.547 kb on + strand, within Pf1N1B4_4388 at 4846.547 kb on + strand, within Pf1N1B4_4388 at 4846.548 kb on - strand, within Pf1N1B4_4388 at 4846.548 kb on - strand, within Pf1N1B4_4388 at 4846.548 kb on - strand, within Pf1N1B4_4388 at 4846.694 kb on + strand, within Pf1N1B4_4388 at 4846.695 kb on - strand at 4846.787 kb on + strand, within Pf1N1B4_4388 at 4846.787 kb on + strand, within Pf1N1B4_4388 at 4846.788 kb on - strand, within Pf1N1B4_4388 at 4847.240 kb on + strand, within Pf1N1B4_4388 at 4847.240 kb on + strand, within Pf1N1B4_4388 at 4847.406 kb on + strand at 4847.406 kb on + strand at 4847.406 kb on + strand at 4847.407 kb on - strand at 4847.407 kb on - strand at 4847.428 kb on + strand at 4847.428 kb on + strand at 4847.429 kb on - strand at 4847.429 kb on - strand at 4847.429 kb on - strand at 4847.584 kb on + strand at 4847.597 kb on + strand at 4847.597 kb on + strand at 4847.629 kb on + strand at 4847.658 kb on + strand at 4847.658 kb on + strand at 4847.658 kb on + strand at 4847.658 kb on + strand at 4847.658 kb on + strand at 4847.659 kb on - strand at 4847.659 kb on - strand at 4847.659 kb on - strand at 4847.929 kb on - strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.221 kb on + strand, within Pf1N1B4_4389 at 4848.221 kb on + strand, within Pf1N1B4_4389
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 4,845,408 - +0.1 4,845,463 + +1.5 4,845,510 + +0.5 4,845,511 - -0.5 4,845,541 - -0.5 4,845,570 + -2.3 4,845,571 - +1.0 4,845,571 - -1.0 4,845,956 - Pf1N1B4_4387 0.44 +0.3 4,846,547 + Pf1N1B4_4388 0.15 -1.0 4,846,547 + Pf1N1B4_4388 0.15 -0.9 4,846,547 + Pf1N1B4_4388 0.15 -1.7 4,846,548 - Pf1N1B4_4388 0.15 -2.6 4,846,548 - Pf1N1B4_4388 0.15 -2.6 4,846,548 - Pf1N1B4_4388 0.15 -1.0 4,846,694 + Pf1N1B4_4388 0.30 -2.5 4,846,695 - -0.0 4,846,787 + Pf1N1B4_4388 0.39 -2.0 4,846,787 + Pf1N1B4_4388 0.39 -1.0 4,846,788 - Pf1N1B4_4388 0.39 +2.3 4,847,240 + Pf1N1B4_4388 0.83 +0.3 4,847,240 + Pf1N1B4_4388 0.83 +1.0 4,847,406 + -0.1 4,847,406 + -0.5 4,847,406 + +0.7 4,847,407 - -0.6 4,847,407 - -0.1 4,847,428 + +1.3 4,847,428 + -0.3 4,847,429 - +0.5 4,847,429 - +0.1 4,847,429 - +0.3 4,847,584 + -0.3 4,847,597 + +0.5 4,847,597 + +0.2 4,847,629 + -1.0 4,847,658 + -0.0 4,847,658 + -0.1 4,847,658 + -1.0 4,847,658 + +0.5 4,847,658 + -0.3 4,847,659 - -1.0 4,847,659 - -0.5 4,847,659 - +0.5 4,847,929 - Pf1N1B4_4389 0.16 -0.0 4,848,081 + Pf1N1B4_4389 0.23 -0.2 4,848,081 + Pf1N1B4_4389 0.23 -1.0 4,848,081 + Pf1N1B4_4389 0.23 +0.1 4,848,081 + Pf1N1B4_4389 0.23 -0.1 4,848,081 + Pf1N1B4_4389 0.23 -0.5 4,848,081 + Pf1N1B4_4389 0.23 +0.5 4,848,081 + Pf1N1B4_4389 0.23 -1.5 4,848,221 + Pf1N1B4_4389 0.30 +1.0 4,848,221 + Pf1N1B4_4389 0.30 -2.3
Or see this region's nucleotide sequence