Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4362

Experiment: Choline Lysine 6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4361 and Pf1N1B4_4362 are separated by 629 nucleotidesPf1N1B4_4362 and Pf1N1B4_4363 are separated by 77 nucleotidesPf1N1B4_4363 and Pf1N1B4_4364 are separated by 208 nucleotides Pf1N1B4_4361 - PvcB protein, related to amino acid oxidizing enzymes, at 4,818,631 to 4,820,391 _4361 Pf1N1B4_4362 - hypothetical protein, at 4,821,021 to 4,821,722 _4362 Pf1N1B4_4363 - hypothetical protein, at 4,821,800 to 4,821,931 _4363 Pf1N1B4_4364 - hypothetical protein, at 4,822,140 to 4,823,612 _4364 Position (kb) 4821 4822Strain fitness (log2 ratio) -2 -1 0 1 2at 4820.056 kb on + strand, within Pf1N1B4_4361at 4820.057 kb on - strand, within Pf1N1B4_4361at 4820.057 kb on - strand, within Pf1N1B4_4361at 4820.057 kb on - strand, within Pf1N1B4_4361at 4820.059 kb on + strand, within Pf1N1B4_4361at 4820.059 kb on + strand, within Pf1N1B4_4361at 4820.060 kb on - strand, within Pf1N1B4_4361at 4820.060 kb on - strand, within Pf1N1B4_4361at 4820.074 kb on + strand, within Pf1N1B4_4361at 4820.075 kb on - strand, within Pf1N1B4_4361at 4820.075 kb on - strand, within Pf1N1B4_4361at 4820.098 kb on + strand, within Pf1N1B4_4361at 4820.099 kb on - strand, within Pf1N1B4_4361at 4820.251 kb on + strandat 4820.503 kb on + strandat 4820.504 kb on - strandat 4820.558 kb on + strandat 4820.561 kb on + strandat 4820.561 kb on + strandat 4820.561 kb on + strandat 4820.567 kb on + strandat 4820.622 kb on + strandat 4820.622 kb on + strandat 4820.655 kb on + strandat 4820.655 kb on + strandat 4820.821 kb on - strandat 4820.821 kb on - strandat 4820.937 kb on + strandat 4821.034 kb on + strandat 4821.211 kb on + strand, within Pf1N1B4_4362at 4821.211 kb on + strand, within Pf1N1B4_4362at 4821.212 kb on - strand, within Pf1N1B4_4362at 4821.241 kb on + strand, within Pf1N1B4_4362at 4821.241 kb on + strand, within Pf1N1B4_4362at 4821.242 kb on - strandat 4821.327 kb on + strand, within Pf1N1B4_4362at 4821.327 kb on + strand, within Pf1N1B4_4362at 4821.327 kb on + strand, within Pf1N1B4_4362at 4821.332 kb on - strand, within Pf1N1B4_4362at 4821.564 kb on - strand, within Pf1N1B4_4362at 4821.564 kb on - strand, within Pf1N1B4_4362at 4821.673 kb on + strandat 4821.754 kb on + strandat 4821.754 kb on + strandat 4821.754 kb on + strandat 4821.755 kb on - strandat 4821.829 kb on + strand, within Pf1N1B4_4363at 4821.829 kb on + strand, within Pf1N1B4_4363at 4821.829 kb on + strand, within Pf1N1B4_4363at 4821.829 kb on + strand, within Pf1N1B4_4363at 4821.829 kb on + strand, within Pf1N1B4_4363at 4821.829 kb on + strand, within Pf1N1B4_4363at 4821.830 kb on - strand, within Pf1N1B4_4363at 4821.830 kb on - strand, within Pf1N1B4_4363at 4821.900 kb on + strand, within Pf1N1B4_4363at 4821.900 kb on + strand, within Pf1N1B4_4363at 4821.900 kb on + strand, within Pf1N1B4_4363at 4821.901 kb on - strand, within Pf1N1B4_4363at 4821.901 kb on - strand, within Pf1N1B4_4363at 4821.901 kb on - strand, within Pf1N1B4_4363at 4821.901 kb on - strand, within Pf1N1B4_4363at 4821.954 kb on - strandat 4822.192 kb on + strandat 4822.193 kb on - strandat 4822.234 kb on + strandat 4822.235 kb on - strandat 4822.235 kb on - strandat 4822.235 kb on - strandat 4822.235 kb on - strandat 4822.235 kb on - strandat 4822.235 kb on - strandat 4822.249 kb on + strandat 4822.250 kb on - strandat 4822.310 kb on - strandat 4822.348 kb on + strandat 4822.348 kb on + strand, within Pf1N1B4_4364at 4822.348 kb on + strand, within Pf1N1B4_4364at 4822.398 kb on - strand, within Pf1N1B4_4364at 4822.637 kb on - strand, within Pf1N1B4_4364at 4822.678 kb on + strand, within Pf1N1B4_4364at 4822.716 kb on + strand, within Pf1N1B4_4364at 4822.717 kb on - strand, within Pf1N1B4_4364at 4822.717 kb on - strand, within Pf1N1B4_4364at 4822.717 kb on - strand, within Pf1N1B4_4364

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 6 mM
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4,820,056 + Pf1N1B4_4361 0.81 +0.8
4,820,057 - Pf1N1B4_4361 0.81 +0.4
4,820,057 - Pf1N1B4_4361 0.81 -0.4
4,820,057 - Pf1N1B4_4361 0.81 +1.0
4,820,059 + Pf1N1B4_4361 0.81 -1.3
4,820,059 + Pf1N1B4_4361 0.81 +0.3
4,820,060 - Pf1N1B4_4361 0.81 +0.1
4,820,060 - Pf1N1B4_4361 0.81 -1.0
4,820,074 + Pf1N1B4_4361 0.82 +0.3
4,820,075 - Pf1N1B4_4361 0.82 -0.0
4,820,075 - Pf1N1B4_4361 0.82 +0.1
4,820,098 + Pf1N1B4_4361 0.83 -1.4
4,820,099 - Pf1N1B4_4361 0.83 -0.6
4,820,251 + -2.0
4,820,503 + +0.6
4,820,504 - -1.0
4,820,558 + -1.8
4,820,561 + -0.4
4,820,561 + +1.4
4,820,561 + +1.1
4,820,567 + -0.4
4,820,622 + -1.0
4,820,622 + -2.0
4,820,655 + +1.4
4,820,655 + -0.6
4,820,821 - +0.6
4,820,821 - -0.2
4,820,937 + +1.3
4,821,034 + +0.6
4,821,211 + Pf1N1B4_4362 0.27 -1.3
4,821,211 + Pf1N1B4_4362 0.27 +0.6
4,821,212 - Pf1N1B4_4362 0.27 -2.0
4,821,241 + Pf1N1B4_4362 0.31 -2.4
4,821,241 + Pf1N1B4_4362 0.31 -1.4
4,821,242 - +0.6
4,821,327 + Pf1N1B4_4362 0.44 -0.7
4,821,327 + Pf1N1B4_4362 0.44 +1.1
4,821,327 + Pf1N1B4_4362 0.44 -0.4
4,821,332 - Pf1N1B4_4362 0.44 +0.4
4,821,564 - Pf1N1B4_4362 0.77 -0.4
4,821,564 - Pf1N1B4_4362 0.77 -0.7
4,821,673 + -0.7
4,821,754 + -0.4
4,821,754 + +2.1
4,821,754 + +0.6
4,821,755 - +0.2
4,821,829 + Pf1N1B4_4363 0.22 -0.2
4,821,829 + Pf1N1B4_4363 0.22 -0.7
4,821,829 + Pf1N1B4_4363 0.22 -1.2
4,821,829 + Pf1N1B4_4363 0.22 +1.0
4,821,829 + Pf1N1B4_4363 0.22 +0.6
4,821,829 + Pf1N1B4_4363 0.22 -0.5
4,821,830 - Pf1N1B4_4363 0.23 +0.3
4,821,830 - Pf1N1B4_4363 0.23 -0.7
4,821,900 + Pf1N1B4_4363 0.76 -0.5
4,821,900 + Pf1N1B4_4363 0.76 -1.0
4,821,900 + Pf1N1B4_4363 0.76 +0.9
4,821,901 - Pf1N1B4_4363 0.77 -1.3
4,821,901 - Pf1N1B4_4363 0.77 -1.4
4,821,901 - Pf1N1B4_4363 0.77 +1.1
4,821,901 - Pf1N1B4_4363 0.77 -0.5
4,821,954 - -0.4
4,822,192 + +1.1
4,822,193 - -1.0
4,822,234 + -0.2
4,822,235 - -0.8
4,822,235 - +0.6
4,822,235 - -0.9
4,822,235 - +0.6
4,822,235 - -0.4
4,822,235 - +1.2
4,822,249 + -0.0
4,822,250 - -1.8
4,822,310 - +1.0
4,822,348 + +2.4
4,822,348 + Pf1N1B4_4364 0.14 +1.0
4,822,348 + Pf1N1B4_4364 0.14 -0.4
4,822,398 - Pf1N1B4_4364 0.18 -0.3
4,822,637 - Pf1N1B4_4364 0.34 +0.4
4,822,678 + Pf1N1B4_4364 0.37 -1.0
4,822,716 + Pf1N1B4_4364 0.39 -2.2
4,822,717 - Pf1N1B4_4364 0.39 +1.7
4,822,717 - Pf1N1B4_4364 0.39 +0.4
4,822,717 - Pf1N1B4_4364 0.39 -2.0

Or see this region's nucleotide sequence