Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4357 and Pf1N1B4_4358 are separated by 405 nucleotides Pf1N1B4_4358 and Pf1N1B4_4359 are separated by 180 nucleotides Pf1N1B4_4359 and Pf1N1B4_4360 are separated by 11 nucleotides Pf1N1B4_4360 and Pf1N1B4_4361 are separated by 27 nucleotides
Pf1N1B4_4357 - Putrescine utilization regulator, at 4,815,395 to 4,815,943
_4357
Pf1N1B4_4358 - Acetyltransferase, GNAT family, at 4,816,349 to 4,816,711
_4358
Pf1N1B4_4359 - PvcA protein, related to known isonitrile synthases, at 4,816,892 to 4,817,887
_4359
Pf1N1B4_4360 - Phosphoglycolate phosphatase (EC 3.1.3.18), at 4,817,899 to 4,818,603
_4360
Pf1N1B4_4361 - PvcB protein, related to amino acid oxidizing enzymes, at 4,818,631 to 4,820,391
_4361
Position (kb)
4816
4817
4818 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4816.008 kb on + strand at 4816.169 kb on + strand at 4816.169 kb on + strand at 4816.170 kb on - strand at 4816.170 kb on - strand at 4816.209 kb on - strand at 4816.209 kb on - strand at 4816.334 kb on + strand at 4816.335 kb on - strand at 4816.337 kb on - strand at 4816.386 kb on - strand, within Pf1N1B4_4358 at 4816.538 kb on + strand, within Pf1N1B4_4358 at 4816.648 kb on + strand, within Pf1N1B4_4358 at 4816.648 kb on + strand, within Pf1N1B4_4358 at 4816.652 kb on + strand, within Pf1N1B4_4358 at 4816.728 kb on + strand at 4816.840 kb on + strand at 4816.892 kb on - strand at 4816.892 kb on - strand at 4817.202 kb on + strand, within Pf1N1B4_4359 at 4817.202 kb on + strand, within Pf1N1B4_4359 at 4817.203 kb on - strand, within Pf1N1B4_4359 at 4817.203 kb on - strand at 4817.203 kb on - strand at 4817.234 kb on + strand, within Pf1N1B4_4359 at 4817.234 kb on + strand, within Pf1N1B4_4359 at 4817.234 kb on + strand, within Pf1N1B4_4359 at 4817.235 kb on - strand, within Pf1N1B4_4359 at 4817.273 kb on + strand, within Pf1N1B4_4359 at 4817.274 kb on - strand at 4817.321 kb on + strand, within Pf1N1B4_4359 at 4817.510 kb on + strand, within Pf1N1B4_4359 at 4817.544 kb on - strand, within Pf1N1B4_4359 at 4817.559 kb on + strand, within Pf1N1B4_4359 at 4817.871 kb on - strand at 4817.915 kb on + strand at 4818.031 kb on + strand, within Pf1N1B4_4360 at 4818.115 kb on + strand, within Pf1N1B4_4360 at 4818.439 kb on + strand, within Pf1N1B4_4360 at 4818.591 kb on + strand at 4818.598 kb on + strand at 4818.599 kb on - strand at 4818.599 kb on - strand at 4818.605 kb on - strand at 4818.639 kb on + strand at 4818.640 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 4,816,008 + -0.8 4,816,169 + -0.4 4,816,169 + -1.8 4,816,170 - -0.1 4,816,170 - -0.7 4,816,209 - +0.1 4,816,209 - -0.2 4,816,334 + +1.6 4,816,335 - +1.1 4,816,337 - -1.0 4,816,386 - Pf1N1B4_4358 0.10 -2.3 4,816,538 + Pf1N1B4_4358 0.52 -0.2 4,816,648 + Pf1N1B4_4358 0.82 -1.3 4,816,648 + Pf1N1B4_4358 0.82 +1.0 4,816,652 + Pf1N1B4_4358 0.83 -0.8 4,816,728 + -0.4 4,816,840 + -2.0 4,816,892 - -0.4 4,816,892 - +1.0 4,817,202 + Pf1N1B4_4359 0.31 -1.4 4,817,202 + Pf1N1B4_4359 0.31 +1.8 4,817,203 - Pf1N1B4_4359 0.31 -1.0 4,817,203 - +1.8 4,817,203 - +1.7 4,817,234 + Pf1N1B4_4359 0.34 +0.1 4,817,234 + Pf1N1B4_4359 0.34 -1.0 4,817,234 + Pf1N1B4_4359 0.34 +0.2 4,817,235 - Pf1N1B4_4359 0.34 +0.6 4,817,273 + Pf1N1B4_4359 0.38 -0.1 4,817,274 - +0.6 4,817,321 + Pf1N1B4_4359 0.43 -0.4 4,817,510 + Pf1N1B4_4359 0.62 -1.3 4,817,544 - Pf1N1B4_4359 0.65 -1.0 4,817,559 + Pf1N1B4_4359 0.67 +1.1 4,817,871 - +1.4 4,817,915 + -0.5 4,818,031 + Pf1N1B4_4360 0.19 +1.6 4,818,115 + Pf1N1B4_4360 0.31 -1.0 4,818,439 + Pf1N1B4_4360 0.77 +1.4 4,818,591 + -0.8 4,818,598 + +0.6 4,818,599 - +1.1 4,818,599 - -0.7 4,818,605 - -0.8 4,818,639 + +0.3 4,818,640 - -0.4
Or see this region's nucleotide sequence