Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4282 and Pf1N1B4_4283 are separated by 4 nucleotides Pf1N1B4_4283 and Pf1N1B4_4284 are separated by 33 nucleotides Pf1N1B4_4284 and Pf1N1B4_4285 are separated by 78 nucleotides Pf1N1B4_4285 and Pf1N1B4_4286 are separated by 54 nucleotides
Pf1N1B4_4282 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 4,748,892 to 4,749,944
_4282
Pf1N1B4_4283 - hypothetical protein, at 4,749,949 to 4,750,065
_4283
Pf1N1B4_4284 - hypothetical protein, at 4,750,099 to 4,750,233
_4284
Pf1N1B4_4285 - Inositol transport system sugar-binding protein, at 4,750,312 to 4,751,238
_4285
Pf1N1B4_4286 - Inositol transport system ATP-binding protein, at 4,751,293 to 4,752,873
_4286
Position (kb)
4750
4751
4752 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4749.457 kb on - strand, within Pf1N1B4_4282 at 4749.471 kb on + strand, within Pf1N1B4_4282 at 4749.509 kb on + strand, within Pf1N1B4_4282 at 4749.510 kb on - strand at 4749.510 kb on - strand, within Pf1N1B4_4282 at 4749.510 kb on - strand, within Pf1N1B4_4282 at 4749.613 kb on - strand, within Pf1N1B4_4282 at 4749.781 kb on - strand, within Pf1N1B4_4282 at 4749.781 kb on - strand, within Pf1N1B4_4282 at 4749.790 kb on + strand, within Pf1N1B4_4282 at 4750.121 kb on - strand, within Pf1N1B4_4284 at 4750.221 kb on - strand at 4750.279 kb on + strand at 4750.383 kb on + strand at 4750.432 kb on + strand, within Pf1N1B4_4285 at 4750.535 kb on + strand, within Pf1N1B4_4285 at 4750.645 kb on + strand at 4750.645 kb on + strand, within Pf1N1B4_4285 at 4750.645 kb on + strand, within Pf1N1B4_4285 at 4750.739 kb on - strand, within Pf1N1B4_4285 at 4750.849 kb on + strand, within Pf1N1B4_4285 at 4751.236 kb on + strand at 4751.237 kb on - strand at 4751.261 kb on + strand at 4751.261 kb on + strand at 4751.262 kb on - strand at 4751.262 kb on - strand at 4751.262 kb on - strand at 4751.299 kb on + strand at 4751.383 kb on + strand at 4751.536 kb on + strand, within Pf1N1B4_4286 at 4751.849 kb on - strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.963 kb on - strand, within Pf1N1B4_4286 at 4751.963 kb on - strand, within Pf1N1B4_4286 at 4751.963 kb on - strand, within Pf1N1B4_4286 at 4752.031 kb on + strand, within Pf1N1B4_4286 at 4752.031 kb on + strand, within Pf1N1B4_4286 at 4752.031 kb on + strand, within Pf1N1B4_4286 at 4752.032 kb on - strand, within Pf1N1B4_4286 at 4752.032 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 4,749,457 - Pf1N1B4_4282 0.54 -0.0 4,749,471 + Pf1N1B4_4282 0.55 -1.3 4,749,509 + Pf1N1B4_4282 0.59 +0.1 4,749,510 - +1.6 4,749,510 - Pf1N1B4_4282 0.59 -1.0 4,749,510 - Pf1N1B4_4282 0.59 +0.1 4,749,613 - Pf1N1B4_4282 0.68 +0.6 4,749,781 - Pf1N1B4_4282 0.84 -1.0 4,749,781 - Pf1N1B4_4282 0.84 -0.0 4,749,790 + Pf1N1B4_4282 0.85 -0.2 4,750,121 - Pf1N1B4_4284 0.16 -0.4 4,750,221 - +0.6 4,750,279 + +1.0 4,750,383 + -0.4 4,750,432 + Pf1N1B4_4285 0.13 -0.2 4,750,535 + Pf1N1B4_4285 0.24 +0.8 4,750,645 + +0.2 4,750,645 + Pf1N1B4_4285 0.36 -1.5 4,750,645 + Pf1N1B4_4285 0.36 -1.8 4,750,739 - Pf1N1B4_4285 0.46 -1.2 4,750,849 + Pf1N1B4_4285 0.58 -0.8 4,751,236 + +0.6 4,751,237 - +0.6 4,751,261 + -1.0 4,751,261 + +1.6 4,751,262 - -0.4 4,751,262 - +0.6 4,751,262 - -1.4 4,751,299 + +1.5 4,751,383 + -0.8 4,751,536 + Pf1N1B4_4286 0.15 +1.6 4,751,849 - Pf1N1B4_4286 0.35 -2.8 4,751,962 + Pf1N1B4_4286 0.42 -1.5 4,751,962 + Pf1N1B4_4286 0.42 +1.0 4,751,962 + Pf1N1B4_4286 0.42 -1.4 4,751,962 + Pf1N1B4_4286 0.42 +1.3 4,751,962 + Pf1N1B4_4286 0.42 +0.3 4,751,963 - Pf1N1B4_4286 0.42 -1.0 4,751,963 - Pf1N1B4_4286 0.42 -0.8 4,751,963 - Pf1N1B4_4286 0.42 +0.6 4,752,031 + Pf1N1B4_4286 0.47 +0.6 4,752,031 + Pf1N1B4_4286 0.47 +0.6 4,752,031 + Pf1N1B4_4286 0.47 +0.2 4,752,032 - Pf1N1B4_4286 0.47 -0.8 4,752,032 - +0.6
Or see this region's nucleotide sequence