Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3963 and Pf1N1B4_3964 are separated by 2 nucleotides Pf1N1B4_3964 and Pf1N1B4_3965 are separated by 231 nucleotides
Pf1N1B4_3963 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 4,402,618 to 4,404,009
_3963
Pf1N1B4_3964 - VgrG protein, at 4,404,012 to 4,405,436
_3964
Pf1N1B4_3965 - FIG033889: YebC paralog in Betaproteobacteria, at 4,405,668 to 4,406,372
_3965
Position (kb)
4404
4405
4406 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4403.099 kb on + strand, within Pf1N1B4_3963 at 4403.099 kb on + strand, within Pf1N1B4_3963 at 4403.100 kb on - strand, within Pf1N1B4_3963 at 4403.100 kb on - strand, within Pf1N1B4_3963 at 4403.100 kb on - strand, within Pf1N1B4_3963 at 4403.107 kb on - strand, within Pf1N1B4_3963 at 4403.252 kb on + strand, within Pf1N1B4_3963 at 4403.253 kb on - strand, within Pf1N1B4_3963 at 4403.476 kb on + strand, within Pf1N1B4_3963 at 4403.476 kb on + strand, within Pf1N1B4_3963 at 4403.476 kb on + strand, within Pf1N1B4_3963 at 4403.476 kb on + strand, within Pf1N1B4_3963 at 4403.477 kb on - strand, within Pf1N1B4_3963 at 4403.526 kb on + strand, within Pf1N1B4_3963 at 4403.646 kb on + strand at 4403.684 kb on + strand, within Pf1N1B4_3963 at 4403.725 kb on + strand, within Pf1N1B4_3963 at 4403.876 kb on - strand at 4404.054 kb on + strand at 4404.355 kb on + strand, within Pf1N1B4_3964 at 4404.434 kb on + strand at 4404.435 kb on - strand, within Pf1N1B4_3964 at 4404.435 kb on - strand, within Pf1N1B4_3964 at 4404.435 kb on - strand, within Pf1N1B4_3964 at 4404.435 kb on - strand, within Pf1N1B4_3964 at 4404.435 kb on - strand, within Pf1N1B4_3964 at 4404.436 kb on + strand at 4404.437 kb on - strand, within Pf1N1B4_3964 at 4404.907 kb on - strand, within Pf1N1B4_3964 at 4404.907 kb on - strand, within Pf1N1B4_3964 at 4404.938 kb on - strand at 4404.994 kb on + strand, within Pf1N1B4_3964 at 4404.994 kb on + strand, within Pf1N1B4_3964 at 4404.995 kb on - strand, within Pf1N1B4_3964 at 4405.178 kb on - strand, within Pf1N1B4_3964 at 4405.632 kb on + strand at 4405.632 kb on + strand at 4405.632 kb on + strand at 4405.632 kb on + strand at 4405.632 kb on + strand at 4405.632 kb on + strand at 4405.632 kb on + strand at 4405.633 kb on - strand at 4405.633 kb on - strand at 4405.914 kb on + strand, within Pf1N1B4_3965 at 4405.914 kb on + strand, within Pf1N1B4_3965 at 4405.914 kb on + strand at 4406.348 kb on + strand at 4406.349 kb on - strand at 4406.370 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 4,403,099 + Pf1N1B4_3963 0.35 +0.1 4,403,099 + Pf1N1B4_3963 0.35 -1.3 4,403,100 - Pf1N1B4_3963 0.35 -1.6 4,403,100 - Pf1N1B4_3963 0.35 +0.5 4,403,100 - Pf1N1B4_3963 0.35 +0.4 4,403,107 - Pf1N1B4_3963 0.35 -0.4 4,403,252 + Pf1N1B4_3963 0.46 -0.4 4,403,253 - Pf1N1B4_3963 0.46 -1.6 4,403,476 + Pf1N1B4_3963 0.62 +0.7 4,403,476 + Pf1N1B4_3963 0.62 +1.2 4,403,476 + Pf1N1B4_3963 0.62 +0.7 4,403,476 + Pf1N1B4_3963 0.62 +0.5 4,403,477 - Pf1N1B4_3963 0.62 +0.0 4,403,526 + Pf1N1B4_3963 0.65 -0.5 4,403,646 + +2.3 4,403,684 + Pf1N1B4_3963 0.77 -0.3 4,403,725 + Pf1N1B4_3963 0.80 +0.7 4,403,876 - -0.6 4,404,054 + -1.0 4,404,355 + Pf1N1B4_3964 0.24 -1.6 4,404,434 + -1.6 4,404,435 - Pf1N1B4_3964 0.30 -1.0 4,404,435 - Pf1N1B4_3964 0.30 -0.5 4,404,435 - Pf1N1B4_3964 0.30 +0.7 4,404,435 - Pf1N1B4_3964 0.30 -1.6 4,404,435 - Pf1N1B4_3964 0.30 +0.6 4,404,436 + +1.3 4,404,437 - Pf1N1B4_3964 0.30 -0.5 4,404,907 - Pf1N1B4_3964 0.63 -0.4 4,404,907 - Pf1N1B4_3964 0.63 -1.2 4,404,938 - -1.3 4,404,994 + Pf1N1B4_3964 0.69 +0.7 4,404,994 + Pf1N1B4_3964 0.69 +0.1 4,404,995 - Pf1N1B4_3964 0.69 -2.3 4,405,178 - Pf1N1B4_3964 0.82 -1.0 4,405,632 + -0.3 4,405,632 + -0.3 4,405,632 + -0.3 4,405,632 + -0.7 4,405,632 + -1.6 4,405,632 + -0.2 4,405,632 + -0.9 4,405,633 - -1.9 4,405,633 - -0.5 4,405,914 + Pf1N1B4_3965 0.35 -1.5 4,405,914 + Pf1N1B4_3965 0.35 -1.0 4,405,914 + +2.3 4,406,348 + -0.6 4,406,349 - +0.1 4,406,370 + -1.0
Or see this region's nucleotide sequence