Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3940 and Pf1N1B4_3941 are separated by 320 nucleotides Pf1N1B4_3941 and Pf1N1B4_3942 are separated by 53 nucleotides Pf1N1B4_3942 and Pf1N1B4_3943 are separated by 238 nucleotides
Pf1N1B4_3940 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 4,375,892 to 4,376,620
_3940
Pf1N1B4_3941 - hypothetical protein, at 4,376,941 to 4,377,165
_3941
Pf1N1B4_3942 - Murein-DD-endopeptidase (EC 3.4.99.-), at 4,377,219 to 4,378,157
_3942
Pf1N1B4_3943 - Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), at 4,378,396 to 4,379,250
_3943
Position (kb)
4377
4378
4379 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4376.672 kb on + strand at 4376.673 kb on - strand at 4376.854 kb on + strand at 4376.854 kb on + strand at 4376.855 kb on - strand at 4376.855 kb on - strand at 4376.895 kb on + strand at 4376.940 kb on + strand at 4376.940 kb on + strand at 4376.940 kb on + strand at 4376.940 kb on + strand at 4376.940 kb on + strand at 4376.940 kb on + strand at 4376.941 kb on - strand at 4376.941 kb on - strand at 4376.941 kb on - strand at 4376.941 kb on - strand at 4377.220 kb on + strand at 4377.221 kb on - strand at 4377.277 kb on + strand at 4377.277 kb on + strand at 4377.277 kb on + strand at 4377.277 kb on + strand at 4377.278 kb on - strand at 4377.278 kb on - strand at 4377.278 kb on - strand at 4377.278 kb on - strand at 4377.278 kb on - strand at 4377.278 kb on - strand at 4377.278 kb on - strand at 4377.362 kb on - strand at 4377.362 kb on - strand, within Pf1N1B4_3942 at 4377.362 kb on - strand, within Pf1N1B4_3942 at 4377.482 kb on + strand, within Pf1N1B4_3942 at 4377.526 kb on + strand, within Pf1N1B4_3942 at 4377.526 kb on + strand, within Pf1N1B4_3942 at 4377.526 kb on + strand, within Pf1N1B4_3942 at 4377.526 kb on + strand, within Pf1N1B4_3942 at 4377.527 kb on - strand, within Pf1N1B4_3942 at 4377.527 kb on - strand at 4377.733 kb on + strand, within Pf1N1B4_3942 at 4377.919 kb on - strand, within Pf1N1B4_3942 at 4377.988 kb on + strand at 4377.988 kb on + strand, within Pf1N1B4_3942 at 4377.988 kb on + strand, within Pf1N1B4_3942 at 4377.988 kb on + strand, within Pf1N1B4_3942 at 4378.184 kb on + strand at 4378.185 kb on - strand at 4378.228 kb on - strand at 4378.228 kb on - strand at 4378.233 kb on + strand at 4378.234 kb on - strand at 4378.292 kb on + strand at 4378.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 4,376,672 + +0.7 4,376,673 - +0.8 4,376,854 + -3.4 4,376,854 + -1.4 4,376,855 - +0.9 4,376,855 - -1.1 4,376,895 + -1.9 4,376,940 + -1.4 4,376,940 + -1.3 4,376,940 + -0.0 4,376,940 + -1.6 4,376,940 + -0.1 4,376,940 + -0.5 4,376,941 - -1.3 4,376,941 - -0.6 4,376,941 - +2.7 4,376,941 - +0.9 4,377,220 + +1.0 4,377,221 - -0.0 4,377,277 + -0.9 4,377,277 + +0.4 4,377,277 + -1.3 4,377,277 + -0.6 4,377,278 - +0.0 4,377,278 - -0.7 4,377,278 - +0.3 4,377,278 - +0.9 4,377,278 - -0.3 4,377,278 - +0.2 4,377,278 - +3.5 4,377,362 - -0.3 4,377,362 - Pf1N1B4_3942 0.15 -1.6 4,377,362 - Pf1N1B4_3942 0.15 -0.8 4,377,482 + Pf1N1B4_3942 0.28 -0.6 4,377,526 + Pf1N1B4_3942 0.33 +1.3 4,377,526 + Pf1N1B4_3942 0.33 -1.9 4,377,526 + Pf1N1B4_3942 0.33 -0.6 4,377,526 + Pf1N1B4_3942 0.33 -1.3 4,377,527 - Pf1N1B4_3942 0.33 -0.5 4,377,527 - -0.9 4,377,733 + Pf1N1B4_3942 0.55 +0.4 4,377,919 - Pf1N1B4_3942 0.75 -0.6 4,377,988 + +0.7 4,377,988 + Pf1N1B4_3942 0.82 -1.6 4,377,988 + Pf1N1B4_3942 0.82 -2.1 4,377,988 + Pf1N1B4_3942 0.82 -0.3 4,378,184 + -0.3 4,378,185 - +0.7 4,378,228 - +0.4 4,378,228 - -1.9 4,378,233 + -0.9 4,378,234 - +0.3 4,378,292 + -1.3 4,378,293 - -0.5
Or see this region's nucleotide sequence