Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3781 and Pf1N1B4_3782 are separated by 160 nucleotides Pf1N1B4_3782 and Pf1N1B4_3783 are separated by 235 nucleotides Pf1N1B4_3783 and Pf1N1B4_3784 are separated by 354 nucleotides
Pf1N1B4_3781 - Nucleoside:H+ symporter:Major facilitator superfamily, at 4,190,320 to 4,191,465
_3781
Pf1N1B4_3782 - 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (EC 1.13.11.54), at 4,191,626 to 4,192,171
_3782
Pf1N1B4_3783 - FIG00958237: hypothetical protein, at 4,192,407 to 4,192,685
_3783
Pf1N1B4_3784 - Acetoacetyl-CoA synthetase (EC 6.2.1.16) / Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 4,193,040 to 4,194,878
_3784
Position (kb)
4192
4193 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.572 kb on + strand at 4191.597 kb on - strand at 4191.666 kb on + strand at 4191.666 kb on + strand at 4191.936 kb on + strand, within Pf1N1B4_3782 at 4191.937 kb on - strand, within Pf1N1B4_3782 at 4191.986 kb on + strand, within Pf1N1B4_3782 at 4191.986 kb on + strand, within Pf1N1B4_3782 at 4191.986 kb on + strand at 4191.987 kb on - strand, within Pf1N1B4_3782 at 4192.120 kb on + strand at 4192.120 kb on + strand at 4192.121 kb on - strand at 4192.174 kb on - strand at 4192.174 kb on - strand at 4192.207 kb on + strand at 4192.208 kb on - strand at 4192.208 kb on - strand at 4192.216 kb on + strand at 4192.217 kb on - strand at 4192.217 kb on - strand at 4192.217 kb on - strand at 4192.217 kb on - strand at 4192.217 kb on - strand at 4192.217 kb on - strand at 4192.224 kb on - strand at 4192.309 kb on + strand at 4192.309 kb on + strand at 4192.309 kb on + strand at 4192.309 kb on + strand at 4192.309 kb on + strand at 4192.309 kb on + strand at 4192.309 kb on + strand at 4192.378 kb on + strand at 4192.379 kb on - strand at 4192.387 kb on + strand at 4192.388 kb on - strand at 4192.388 kb on - strand at 4192.388 kb on - strand at 4192.388 kb on - strand at 4192.388 kb on - strand at 4192.391 kb on + strand at 4192.391 kb on + strand at 4192.392 kb on - strand at 4192.392 kb on - strand at 4192.394 kb on - strand at 4192.394 kb on - strand at 4192.394 kb on - strand at 4192.394 kb on - strand at 4192.420 kb on + strand at 4192.420 kb on + strand at 4192.420 kb on + strand at 4192.420 kb on + strand at 4192.420 kb on + strand at 4192.420 kb on + strand at 4192.421 kb on - strand at 4192.504 kb on - strand, within Pf1N1B4_3783 at 4192.504 kb on - strand, within Pf1N1B4_3783 at 4192.553 kb on - strand, within Pf1N1B4_3783 at 4192.653 kb on + strand, within Pf1N1B4_3783 at 4192.962 kb on + strand at 4192.962 kb on + strand at 4192.984 kb on + strand at 4192.984 kb on + strand at 4192.985 kb on - strand at 4193.245 kb on + strand, within Pf1N1B4_3784 at 4193.253 kb on + strand, within Pf1N1B4_3784 at 4193.253 kb on + strand, within Pf1N1B4_3784 at 4193.254 kb on - strand, within Pf1N1B4_3784 at 4193.415 kb on - strand, within Pf1N1B4_3784 at 4193.504 kb on + strand, within Pf1N1B4_3784 at 4193.505 kb on - strand, within Pf1N1B4_3784
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 4,191,444 + +0.8 4,191,444 + +0.0 4,191,444 + -1.1 4,191,444 + +1.5 4,191,444 + -0.3 4,191,444 + -0.7 4,191,444 + +2.1 4,191,444 + -0.2 4,191,444 + +0.8 4,191,445 - -2.4 4,191,445 - -0.2 4,191,445 - -0.8 4,191,445 - -2.0 4,191,445 - +1.1 4,191,445 - -0.0 4,191,445 - -0.0 4,191,445 - -1.2 4,191,572 + -1.2 4,191,597 - -0.5 4,191,666 + +1.6 4,191,666 + +0.8 4,191,936 + Pf1N1B4_3782 0.57 -1.5 4,191,937 - Pf1N1B4_3782 0.57 -0.4 4,191,986 + Pf1N1B4_3782 0.66 -1.8 4,191,986 + Pf1N1B4_3782 0.66 -1.2 4,191,986 + -1.6 4,191,987 - Pf1N1B4_3782 0.66 +0.4 4,192,120 + +0.4 4,192,120 + +0.5 4,192,121 - -0.8 4,192,174 - +1.1 4,192,174 - -0.6 4,192,207 + -1.6 4,192,208 - -1.0 4,192,208 - +1.5 4,192,216 + -0.2 4,192,217 - +2.2 4,192,217 - -0.8 4,192,217 - -0.1 4,192,217 - +0.2 4,192,217 - +1.2 4,192,217 - +1.4 4,192,224 - +0.1 4,192,309 + +2.6 4,192,309 + -0.8 4,192,309 + -1.0 4,192,309 + -0.2 4,192,309 + +0.4 4,192,309 + -0.8 4,192,309 + -0.2 4,192,378 + +0.0 4,192,379 - -2.0 4,192,387 + +0.8 4,192,388 - -0.0 4,192,388 - -0.1 4,192,388 - -0.2 4,192,388 - +1.8 4,192,388 - +0.2 4,192,391 + -1.2 4,192,391 + +0.8 4,192,392 - +1.4 4,192,392 - -0.2 4,192,394 - +0.3 4,192,394 - -0.2 4,192,394 - -1.2 4,192,394 - +0.1 4,192,420 + -0.8 4,192,420 + -0.2 4,192,420 + +0.1 4,192,420 + +2.8 4,192,420 + -0.8 4,192,420 + -0.6 4,192,421 - -1.5 4,192,504 - Pf1N1B4_3783 0.35 -0.8 4,192,504 - Pf1N1B4_3783 0.35 -0.9 4,192,553 - Pf1N1B4_3783 0.52 +1.1 4,192,653 + Pf1N1B4_3783 0.88 -1.1 4,192,962 + +0.1 4,192,962 + +1.9 4,192,984 + -1.2 4,192,984 + -2.1 4,192,985 - -1.2 4,193,245 + Pf1N1B4_3784 0.11 -1.2 4,193,253 + Pf1N1B4_3784 0.12 +1.0 4,193,253 + Pf1N1B4_3784 0.12 +1.2 4,193,254 - Pf1N1B4_3784 0.12 -0.4 4,193,415 - Pf1N1B4_3784 0.20 +1.0 4,193,504 + Pf1N1B4_3784 0.25 -1.6 4,193,505 - Pf1N1B4_3784 0.25 -2.2
Or see this region's nucleotide sequence