Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_376 and Pf1N1B4_377 are separated by 211 nucleotides Pf1N1B4_377 and Pf1N1B4_378 are separated by 5 nucleotides Pf1N1B4_378 and Pf1N1B4_379 are separated by 153 nucleotides Pf1N1B4_379 and Pf1N1B4_380 are separated by 64 nucleotides
Pf1N1B4_376 - Deblocking aminopeptidase (EC 3.4.11.-) @ Cyanophycinase 2 (EC 3.4.15.6), at 447,403 to 448,593
_376
Pf1N1B4_377 - FIG00953242: hypothetical protein, at 448,805 to 449,032
_377
Pf1N1B4_378 - Carbon storage regulator, at 449,038 to 449,223
_378
Pf1N1B4_379 - Protein of unknown function UPF0060, at 449,377 to 449,709
_379
Pf1N1B4_380 - Oxidoreductase, short chain dehydrogenase/reductase family, at 449,774 to 450,589
_380
Position (kb) 449
450 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 448.216 kb on + strand, within Pf1N1B4_376 at 448.222 kb on + strand, within Pf1N1B4_376 at 448.298 kb on - strand, within Pf1N1B4_376 at 448.298 kb on - strand, within Pf1N1B4_376 at 448.298 kb on - strand, within Pf1N1B4_376 at 448.298 kb on - strand, within Pf1N1B4_376 at 448.438 kb on + strand, within Pf1N1B4_376 at 448.438 kb on + strand, within Pf1N1B4_376 at 448.439 kb on - strand, within Pf1N1B4_376 at 448.439 kb on - strand, within Pf1N1B4_376 at 448.439 kb on - strand, within Pf1N1B4_376 at 448.439 kb on - strand, within Pf1N1B4_376 at 448.747 kb on - strand at 448.762 kb on - strand at 448.804 kb on + strand at 448.804 kb on + strand at 449.082 kb on - strand, within Pf1N1B4_378 at 449.082 kb on - strand, within Pf1N1B4_378 at 449.082 kb on - strand, within Pf1N1B4_378 at 449.082 kb on - strand, within Pf1N1B4_378 at 449.098 kb on + strand, within Pf1N1B4_378 at 449.098 kb on + strand, within Pf1N1B4_378 at 449.098 kb on + strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.216 kb on - strand at 449.312 kb on - strand at 449.312 kb on - strand at 449.358 kb on + strand at 449.359 kb on - strand at 449.510 kb on + strand, within Pf1N1B4_379 at 449.510 kb on + strand, within Pf1N1B4_379 at 449.510 kb on + strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.522 kb on + strand, within Pf1N1B4_379 at 449.523 kb on - strand, within Pf1N1B4_379 at 449.625 kb on + strand at 449.625 kb on + strand, within Pf1N1B4_379 at 449.626 kb on - strand, within Pf1N1B4_379 at 449.699 kb on + strand at 449.699 kb on + strand at 449.699 kb on + strand at 449.699 kb on + strand at 449.699 kb on + strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.835 kb on + strand at 449.836 kb on - strand at 449.836 kb on - strand at 449.928 kb on + strand, within Pf1N1B4_380 at 449.934 kb on + strand, within Pf1N1B4_380 at 449.934 kb on + strand, within Pf1N1B4_380 at 449.934 kb on + strand, within Pf1N1B4_380 at 449.935 kb on - strand, within Pf1N1B4_380 at 449.935 kb on - strand, within Pf1N1B4_380 at 449.935 kb on - strand at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 448,216 + Pf1N1B4_376 0.68 +0.4 448,222 + Pf1N1B4_376 0.69 -1.2 448,298 - Pf1N1B4_376 0.75 -0.6 448,298 - Pf1N1B4_376 0.75 -2.3 448,298 - Pf1N1B4_376 0.75 +1.2 448,298 - Pf1N1B4_376 0.75 +0.3 448,438 + Pf1N1B4_376 0.87 -0.6 448,438 + Pf1N1B4_376 0.87 +0.6 448,439 - Pf1N1B4_376 0.87 +0.5 448,439 - Pf1N1B4_376 0.87 +0.4 448,439 - Pf1N1B4_376 0.87 -2.0 448,439 - Pf1N1B4_376 0.87 -0.3 448,747 - +0.4 448,762 - -0.9 448,804 + -2.3 448,804 + +0.6 449,082 - Pf1N1B4_378 0.24 -2.6 449,082 - Pf1N1B4_378 0.24 -1.2 449,082 - Pf1N1B4_378 0.24 -1.7 449,082 - Pf1N1B4_378 0.24 +0.8 449,098 + Pf1N1B4_378 0.32 -2.2 449,098 + Pf1N1B4_378 0.32 -1.0 449,098 + Pf1N1B4_378 0.32 +0.4 449,099 - Pf1N1B4_378 0.33 +0.4 449,099 - Pf1N1B4_378 0.33 +0.0 449,099 - Pf1N1B4_378 0.33 -1.5 449,099 - Pf1N1B4_378 0.33 -0.6 449,099 - Pf1N1B4_378 0.33 -0.2 449,216 - -1.2 449,312 - +0.1 449,312 - +0.1 449,358 + +1.2 449,359 - -0.2 449,510 + Pf1N1B4_379 0.40 +1.8 449,510 + Pf1N1B4_379 0.40 +0.4 449,510 + Pf1N1B4_379 0.40 -1.5 449,511 - Pf1N1B4_379 0.40 -0.5 449,511 - Pf1N1B4_379 0.40 -0.7 449,511 - Pf1N1B4_379 0.40 -0.3 449,511 - Pf1N1B4_379 0.40 -0.0 449,511 - Pf1N1B4_379 0.40 -0.2 449,511 - Pf1N1B4_379 0.40 +0.8 449,522 + Pf1N1B4_379 0.44 -1.0 449,523 - Pf1N1B4_379 0.44 -2.2 449,625 + +1.8 449,625 + Pf1N1B4_379 0.74 -0.2 449,626 - Pf1N1B4_379 0.75 -0.2 449,699 + -0.6 449,699 + -0.2 449,699 + +0.6 449,699 + +1.3 449,699 + +0.6 449,700 - -0.3 449,700 - +1.2 449,700 - -2.2 449,700 - +1.3 449,700 - +1.1 449,700 - -0.2 449,700 - -0.2 449,700 - -0.2 449,835 + +3.4 449,836 - +0.2 449,836 - -1.0 449,928 + Pf1N1B4_380 0.19 -1.7 449,934 + Pf1N1B4_380 0.20 +0.3 449,934 + Pf1N1B4_380 0.20 -0.0 449,934 + Pf1N1B4_380 0.20 +0.8 449,935 - Pf1N1B4_380 0.20 +0.3 449,935 - Pf1N1B4_380 0.20 -1.5 449,935 - +2.4 450,141 + Pf1N1B4_380 0.45 -0.2 450,141 + Pf1N1B4_380 0.45 +1.8 450,141 + Pf1N1B4_380 0.45 -3.5 450,141 + Pf1N1B4_380 0.45 -2.2 450,142 - Pf1N1B4_380 0.45 -0.2 450,142 - Pf1N1B4_380 0.45 -1.7 450,142 - +0.8 450,142 - Pf1N1B4_380 0.45 -0.5 450,142 - Pf1N1B4_380 0.45 +2.8 450,142 - Pf1N1B4_380 0.45 +0.5
Or see this region's nucleotide sequence