Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3656 and Pf1N1B4_3657 are separated by 139 nucleotides Pf1N1B4_3657 and Pf1N1B4_3658 overlap by 11 nucleotides
Pf1N1B4_3656 - Eukaryotic-type low-affinity urea transporter, at 4,060,682 to 4,061,596
_3656
Pf1N1B4_3657 - Pyruvate kinase (EC 2.7.1.40), at 4,061,736 to 4,063,151
_3657
Pf1N1B4_3658 - D-glycerate 2-kinase (EC 2.7.1.-), at 4,063,141 to 4,064,421
_3658
Position (kb)
4061
4062
4063
4064 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4060.940 kb on + strand, within Pf1N1B4_3656 at 4061.326 kb on - strand at 4061.354 kb on - strand, within Pf1N1B4_3656 at 4061.361 kb on + strand, within Pf1N1B4_3656 at 4061.398 kb on - strand, within Pf1N1B4_3656 at 4061.737 kb on + strand at 4061.802 kb on - strand at 4061.813 kb on + strand at 4062.005 kb on + strand, within Pf1N1B4_3657 at 4062.006 kb on - strand at 4062.006 kb on - strand, within Pf1N1B4_3657 at 4062.026 kb on - strand, within Pf1N1B4_3657 at 4062.026 kb on - strand, within Pf1N1B4_3657 at 4062.737 kb on - strand, within Pf1N1B4_3657 at 4062.777 kb on + strand, within Pf1N1B4_3657 at 4062.798 kb on - strand, within Pf1N1B4_3657 at 4062.968 kb on - strand, within Pf1N1B4_3657 at 4063.055 kb on + strand at 4063.139 kb on + strand at 4063.244 kb on + strand at 4063.310 kb on + strand, within Pf1N1B4_3658 at 4063.865 kb on - strand, within Pf1N1B4_3658 at 4063.865 kb on - strand, within Pf1N1B4_3658 at 4063.865 kb on - strand, within Pf1N1B4_3658 at 4063.880 kb on + strand, within Pf1N1B4_3658 at 4063.880 kb on + strand, within Pf1N1B4_3658 at 4063.881 kb on - strand, within Pf1N1B4_3658 at 4063.881 kb on - strand, within Pf1N1B4_3658 at 4063.881 kb on - strand, within Pf1N1B4_3658 at 4063.884 kb on + strand, within Pf1N1B4_3658 at 4063.884 kb on + strand, within Pf1N1B4_3658 at 4063.884 kb on + strand at 4063.884 kb on + strand at 4063.884 kb on + strand, within Pf1N1B4_3658 at 4063.884 kb on + strand, within Pf1N1B4_3658 at 4063.885 kb on - strand, within Pf1N1B4_3658 at 4063.885 kb on - strand, within Pf1N1B4_3658 at 4063.885 kb on - strand, within Pf1N1B4_3658 at 4063.885 kb on - strand, within Pf1N1B4_3658 at 4063.886 kb on + strand, within Pf1N1B4_3658 at 4063.886 kb on + strand, within Pf1N1B4_3658 at 4063.887 kb on - strand, within Pf1N1B4_3658 at 4063.887 kb on - strand, within Pf1N1B4_3658
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 4,060,940 + Pf1N1B4_3656 0.28 +0.6 4,061,326 - -1.2 4,061,354 - Pf1N1B4_3656 0.73 +0.5 4,061,361 + Pf1N1B4_3656 0.74 +2.4 4,061,398 - Pf1N1B4_3656 0.78 +0.8 4,061,737 + -0.7 4,061,802 - -2.4 4,061,813 + -1.8 4,062,005 + Pf1N1B4_3657 0.19 -0.8 4,062,006 - +1.1 4,062,006 - Pf1N1B4_3657 0.19 -0.5 4,062,026 - Pf1N1B4_3657 0.20 +0.0 4,062,026 - Pf1N1B4_3657 0.20 -1.2 4,062,737 - Pf1N1B4_3657 0.71 +0.8 4,062,777 + Pf1N1B4_3657 0.74 -2.7 4,062,798 - Pf1N1B4_3657 0.75 -2.4 4,062,968 - Pf1N1B4_3657 0.87 -0.2 4,063,055 + -0.0 4,063,139 + +1.9 4,063,244 + -0.8 4,063,310 + Pf1N1B4_3658 0.13 -1.0 4,063,865 - Pf1N1B4_3658 0.57 -0.8 4,063,865 - Pf1N1B4_3658 0.57 -0.4 4,063,865 - Pf1N1B4_3658 0.57 -1.4 4,063,880 + Pf1N1B4_3658 0.58 -0.6 4,063,880 + Pf1N1B4_3658 0.58 -0.0 4,063,881 - Pf1N1B4_3658 0.58 -1.2 4,063,881 - Pf1N1B4_3658 0.58 -0.2 4,063,881 - Pf1N1B4_3658 0.58 -1.2 4,063,884 + Pf1N1B4_3658 0.58 +0.1 4,063,884 + Pf1N1B4_3658 0.58 -1.2 4,063,884 + +2.4 4,063,884 + -0.2 4,063,884 + Pf1N1B4_3658 0.58 +0.5 4,063,884 + Pf1N1B4_3658 0.58 -1.2 4,063,885 - Pf1N1B4_3658 0.58 +1.6 4,063,885 - Pf1N1B4_3658 0.58 -0.0 4,063,885 - Pf1N1B4_3658 0.58 +1.3 4,063,885 - Pf1N1B4_3658 0.58 +0.2 4,063,886 + Pf1N1B4_3658 0.58 -0.6 4,063,886 + Pf1N1B4_3658 0.58 +0.9 4,063,887 - Pf1N1B4_3658 0.58 -0.2 4,063,887 - Pf1N1B4_3658 0.58 -0.2
Or see this region's nucleotide sequence