Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3497 and Pf1N1B4_3498 overlap by 1 nucleotides Pf1N1B4_3498 and Pf1N1B4_3499 are separated by 296 nucleotides Pf1N1B4_3499 and Pf1N1B4_3500 are separated by 4 nucleotides Pf1N1B4_3500 and Pf1N1B4_3501 are separated by 199 nucleotides
Pf1N1B4_3497 - Heat shock protein, at 3,902,754 to 3,903,158
_3497
Pf1N1B4_3498 - FIG00953240: hypothetical protein, at 3,903,158 to 3,903,592
_3498
Pf1N1B4_3499 - hypothetical protein, at 3,903,889 to 3,904,113
_3499
Pf1N1B4_3500 - D-lactate dehydrogenase (EC 1.1.1.28), at 3,904,118 to 3,905,107
_3500
Pf1N1B4_3501 - BarA sensory histidine kinase (= VarS = GacS), at 3,905,307 to 3,908,057
_3501
Position (kb)
3904
3905
3906 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3903.452 kb on + strand, within Pf1N1B4_3498 at 3903.462 kb on - strand at 3903.462 kb on - strand, within Pf1N1B4_3498 at 3903.475 kb on + strand, within Pf1N1B4_3498 at 3903.478 kb on + strand, within Pf1N1B4_3498 at 3903.478 kb on + strand, within Pf1N1B4_3498 at 3903.479 kb on - strand, within Pf1N1B4_3498 at 3903.479 kb on - strand, within Pf1N1B4_3498 at 3903.571 kb on - strand at 3903.605 kb on + strand at 3903.605 kb on + strand at 3903.605 kb on + strand at 3903.605 kb on + strand at 3903.606 kb on - strand at 3903.606 kb on - strand at 3903.606 kb on - strand at 3903.614 kb on + strand at 3903.654 kb on + strand at 3903.654 kb on + strand at 3903.655 kb on - strand at 3903.655 kb on - strand at 3903.715 kb on + strand at 3903.786 kb on + strand at 3903.787 kb on - strand at 3903.918 kb on + strand, within Pf1N1B4_3499 at 3903.919 kb on - strand, within Pf1N1B4_3499 at 3904.238 kb on + strand, within Pf1N1B4_3500 at 3904.238 kb on + strand, within Pf1N1B4_3500 at 3904.238 kb on + strand, within Pf1N1B4_3500 at 3904.238 kb on + strand, within Pf1N1B4_3500 at 3904.238 kb on + strand, within Pf1N1B4_3500 at 3904.238 kb on + strand, within Pf1N1B4_3500 at 3904.239 kb on - strand, within Pf1N1B4_3500 at 3904.330 kb on - strand, within Pf1N1B4_3500 at 3904.330 kb on - strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.422 kb on - strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.738 kb on - strand at 3904.809 kb on + strand, within Pf1N1B4_3500 at 3904.809 kb on + strand, within Pf1N1B4_3500 at 3904.810 kb on - strand, within Pf1N1B4_3500 at 3904.929 kb on - strand, within Pf1N1B4_3500 at 3905.076 kb on + strand at 3905.077 kb on - strand at 3905.125 kb on + strand at 3905.125 kb on + strand at 3905.126 kb on - strand at 3905.126 kb on - strand at 3905.132 kb on - strand at 3905.219 kb on - strand at 3905.273 kb on + strand at 3905.295 kb on + strand at 3905.607 kb on + strand, within Pf1N1B4_3501 at 3905.607 kb on + strand at 3905.608 kb on - strand, within Pf1N1B4_3501 at 3905.608 kb on - strand, within Pf1N1B4_3501 at 3905.608 kb on - strand, within Pf1N1B4_3501 at 3905.752 kb on + strand, within Pf1N1B4_3501 at 3905.752 kb on + strand, within Pf1N1B4_3501 at 3905.753 kb on - strand, within Pf1N1B4_3501 at 3905.753 kb on - strand, within Pf1N1B4_3501 at 3905.753 kb on - strand, within Pf1N1B4_3501 at 3905.753 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 3,903,452 + Pf1N1B4_3498 0.68 -0.1 3,903,462 - -0.7 3,903,462 - Pf1N1B4_3498 0.70 +0.2 3,903,475 + Pf1N1B4_3498 0.73 -1.1 3,903,478 + Pf1N1B4_3498 0.74 -0.1 3,903,478 + Pf1N1B4_3498 0.74 +0.3 3,903,479 - Pf1N1B4_3498 0.74 +0.4 3,903,479 - Pf1N1B4_3498 0.74 -2.1 3,903,571 - +0.3 3,903,605 + -0.9 3,903,605 + +0.9 3,903,605 + +0.1 3,903,605 + +2.9 3,903,606 - +2.2 3,903,606 - -0.1 3,903,606 - -0.3 3,903,614 + -0.9 3,903,654 + -1.7 3,903,654 + -0.1 3,903,655 - -0.1 3,903,655 - -1.3 3,903,715 + +0.1 3,903,786 + +1.2 3,903,787 - -0.7 3,903,918 + Pf1N1B4_3499 0.13 -0.8 3,903,919 - Pf1N1B4_3499 0.13 -0.3 3,904,238 + Pf1N1B4_3500 0.12 -2.3 3,904,238 + Pf1N1B4_3500 0.12 +0.4 3,904,238 + Pf1N1B4_3500 0.12 -0.1 3,904,238 + Pf1N1B4_3500 0.12 -1.1 3,904,238 + Pf1N1B4_3500 0.12 +0.5 3,904,238 + Pf1N1B4_3500 0.12 -0.9 3,904,239 - Pf1N1B4_3500 0.12 -0.4 3,904,330 - Pf1N1B4_3500 0.21 -0.7 3,904,330 - Pf1N1B4_3500 0.21 +1.6 3,904,421 + Pf1N1B4_3500 0.31 -2.8 3,904,421 + Pf1N1B4_3500 0.31 +0.2 3,904,421 + Pf1N1B4_3500 0.31 +0.5 3,904,421 + Pf1N1B4_3500 0.31 +0.1 3,904,422 - Pf1N1B4_3500 0.31 -0.8 3,904,737 + Pf1N1B4_3500 0.63 -1.5 3,904,737 + Pf1N1B4_3500 0.63 +0.1 3,904,737 + Pf1N1B4_3500 0.63 -1.1 3,904,737 + Pf1N1B4_3500 0.63 -2.1 3,904,738 - -0.1 3,904,809 + Pf1N1B4_3500 0.70 -1.5 3,904,809 + Pf1N1B4_3500 0.70 +0.7 3,904,810 - Pf1N1B4_3500 0.70 -0.3 3,904,929 - Pf1N1B4_3500 0.82 -0.6 3,905,076 + -0.4 3,905,077 - -0.4 3,905,125 + +1.2 3,905,125 + +1.9 3,905,126 - +0.5 3,905,126 - -1.1 3,905,132 - -0.7 3,905,219 - -0.1 3,905,273 + -0.7 3,905,295 + +1.2 3,905,607 + Pf1N1B4_3501 0.11 -1.1 3,905,607 + +0.9 3,905,608 - Pf1N1B4_3501 0.11 +0.3 3,905,608 - Pf1N1B4_3501 0.11 -0.7 3,905,608 - Pf1N1B4_3501 0.11 +0.2 3,905,752 + Pf1N1B4_3501 0.16 -1.5 3,905,752 + Pf1N1B4_3501 0.16 +0.4 3,905,753 - Pf1N1B4_3501 0.16 -1.5 3,905,753 - Pf1N1B4_3501 0.16 -1.9 3,905,753 - Pf1N1B4_3501 0.16 -1.5 3,905,753 - +2.9
Or see this region's nucleotide sequence