Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3462 and Pf1N1B4_3463 overlap by 4 nucleotides Pf1N1B4_3463 and Pf1N1B4_3464 are separated by 181 nucleotides
Pf1N1B4_3462 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 3,867,906 to 3,869,510
_3462
Pf1N1B4_3463 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 3,869,507 to 3,870,199
_3463
Pf1N1B4_3464 - glutamine synthetase family protein, at 3,870,381 to 3,871,724
_3464
Position (kb)
3869
3870
3871 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3868.533 kb on - strand, within Pf1N1B4_3462 at 3868.566 kb on + strand at 3868.567 kb on - strand, within Pf1N1B4_3462 at 3868.567 kb on - strand, within Pf1N1B4_3462 at 3869.157 kb on + strand, within Pf1N1B4_3462 at 3869.157 kb on + strand, within Pf1N1B4_3462 at 3869.157 kb on + strand, within Pf1N1B4_3462 at 3869.157 kb on + strand, within Pf1N1B4_3462 at 3869.268 kb on + strand, within Pf1N1B4_3462 at 3869.268 kb on + strand, within Pf1N1B4_3462 at 3869.269 kb on - strand, within Pf1N1B4_3462 at 3869.269 kb on - strand, within Pf1N1B4_3462 at 3869.302 kb on + strand, within Pf1N1B4_3462 at 3869.303 kb on - strand, within Pf1N1B4_3462 at 3869.303 kb on - strand, within Pf1N1B4_3462 at 3869.303 kb on - strand, within Pf1N1B4_3462 at 3869.342 kb on + strand, within Pf1N1B4_3462 at 3869.714 kb on + strand, within Pf1N1B4_3463 at 3869.834 kb on + strand, within Pf1N1B4_3463 at 3869.834 kb on + strand, within Pf1N1B4_3463 at 3869.834 kb on + strand, within Pf1N1B4_3463 at 3869.834 kb on + strand, within Pf1N1B4_3463 at 3869.834 kb on + strand, within Pf1N1B4_3463 at 3869.835 kb on - strand, within Pf1N1B4_3463 at 3869.835 kb on - strand, within Pf1N1B4_3463 at 3869.835 kb on - strand, within Pf1N1B4_3463 at 3869.835 kb on - strand, within Pf1N1B4_3463 at 3869.835 kb on - strand, within Pf1N1B4_3463 at 3869.893 kb on - strand, within Pf1N1B4_3463 at 3870.038 kb on + strand, within Pf1N1B4_3463 at 3870.039 kb on - strand, within Pf1N1B4_3463 at 3870.086 kb on + strand, within Pf1N1B4_3463 at 3870.188 kb on + strand at 3870.188 kb on + strand at 3870.189 kb on - strand at 3870.189 kb on - strand at 3870.189 kb on - strand at 3870.189 kb on - strand at 3870.243 kb on + strand at 3870.330 kb on + strand at 3870.330 kb on + strand at 3870.331 kb on - strand at 3870.360 kb on + strand at 3870.361 kb on - strand at 3870.580 kb on - strand, within Pf1N1B4_3464 at 3870.930 kb on + strand, within Pf1N1B4_3464 at 3870.930 kb on + strand at 3871.148 kb on + strand, within Pf1N1B4_3464 at 3871.149 kb on - strand, within Pf1N1B4_3464
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 3,868,533 - Pf1N1B4_3462 0.39 -0.2 3,868,566 + +1.9 3,868,567 - Pf1N1B4_3462 0.41 +0.5 3,868,567 - Pf1N1B4_3462 0.41 -0.7 3,869,157 + Pf1N1B4_3462 0.78 +0.5 3,869,157 + Pf1N1B4_3462 0.78 +0.2 3,869,157 + Pf1N1B4_3462 0.78 -0.9 3,869,157 + Pf1N1B4_3462 0.78 +0.1 3,869,268 + Pf1N1B4_3462 0.85 -1.5 3,869,268 + Pf1N1B4_3462 0.85 -2.6 3,869,269 - Pf1N1B4_3462 0.85 +0.6 3,869,269 - Pf1N1B4_3462 0.85 -0.1 3,869,302 + Pf1N1B4_3462 0.87 -2.5 3,869,303 - Pf1N1B4_3462 0.87 -0.6 3,869,303 - Pf1N1B4_3462 0.87 +0.9 3,869,303 - Pf1N1B4_3462 0.87 +2.9 3,869,342 + Pf1N1B4_3462 0.89 +0.9 3,869,714 + Pf1N1B4_3463 0.30 +0.5 3,869,834 + Pf1N1B4_3463 0.47 -0.2 3,869,834 + Pf1N1B4_3463 0.47 -0.1 3,869,834 + Pf1N1B4_3463 0.47 -1.7 3,869,834 + Pf1N1B4_3463 0.47 -0.1 3,869,834 + Pf1N1B4_3463 0.47 -1.7 3,869,835 - Pf1N1B4_3463 0.47 -0.4 3,869,835 - Pf1N1B4_3463 0.47 -0.9 3,869,835 - Pf1N1B4_3463 0.47 +0.9 3,869,835 - Pf1N1B4_3463 0.47 +0.9 3,869,835 - Pf1N1B4_3463 0.47 -0.9 3,869,893 - Pf1N1B4_3463 0.56 -1.3 3,870,038 + Pf1N1B4_3463 0.77 -0.3 3,870,039 - Pf1N1B4_3463 0.77 -1.1 3,870,086 + Pf1N1B4_3463 0.84 -0.9 3,870,188 + +0.1 3,870,188 + -1.0 3,870,189 - +0.4 3,870,189 - +1.9 3,870,189 - -1.1 3,870,189 - +1.4 3,870,243 + +1.7 3,870,330 + +0.4 3,870,330 + -0.7 3,870,331 - -2.1 3,870,360 + +0.4 3,870,361 - +0.9 3,870,580 - Pf1N1B4_3464 0.15 -1.9 3,870,930 + Pf1N1B4_3464 0.41 -0.5 3,870,930 + +1.9 3,871,148 + Pf1N1B4_3464 0.57 -0.7 3,871,149 - Pf1N1B4_3464 0.57 -0.3
Or see this region's nucleotide sequence