Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_342

Experiment: Choline Lysine 6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_340 and Pf1N1B4_341 are separated by 56 nucleotidesPf1N1B4_341 and Pf1N1B4_342 overlap by 4 nucleotidesPf1N1B4_342 and Pf1N1B4_343 overlap by 4 nucleotides Pf1N1B4_340 - Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-), at 404,113 to 405,036 _340 Pf1N1B4_341 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 405,093 to 405,983 _341 Pf1N1B4_342 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 405,980 to 406,873 _342 Pf1N1B4_343 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 406,870 to 407,946 _343 Position (kb) 405 406 407Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 404.993 kb on + strandat 404.993 kb on + strandat 404.993 kb on + strandat 404.994 kb on - strandat 404.994 kb on - strandat 404.994 kb on - strandat 404.994 kb on - strandat 405.029 kb on - strandat 405.029 kb on - strandat 405.128 kb on - strandat 405.128 kb on - strandat 405.128 kb on - strandat 405.359 kb on - strandat 405.443 kb on - strand, within Pf1N1B4_341at 405.443 kb on - strand, within Pf1N1B4_341at 405.443 kb on - strand, within Pf1N1B4_341at 405.448 kb on + strand, within Pf1N1B4_341at 405.448 kb on + strand, within Pf1N1B4_341at 405.448 kb on + strand, within Pf1N1B4_341at 405.449 kb on - strand, within Pf1N1B4_341at 405.476 kb on + strand, within Pf1N1B4_341at 405.477 kb on - strand, within Pf1N1B4_341at 405.574 kb on - strand, within Pf1N1B4_341at 405.635 kb on - strand, within Pf1N1B4_341at 405.635 kb on - strand, within Pf1N1B4_341at 405.726 kb on - strand, within Pf1N1B4_341at 405.740 kb on - strand, within Pf1N1B4_341at 405.815 kb on + strandat 405.815 kb on + strand, within Pf1N1B4_341at 405.816 kb on - strand, within Pf1N1B4_341at 405.966 kb on - strandat 405.985 kb on + strandat 405.985 kb on + strandat 406.896 kb on - strandat 406.943 kb on - strandat 406.943 kb on - strandat 407.055 kb on - strand, within Pf1N1B4_343at 407.088 kb on - strandat 407.088 kb on - strand, within Pf1N1B4_343at 407.158 kb on - strand, within Pf1N1B4_343at 407.205 kb on - strand, within Pf1N1B4_343at 407.205 kb on - strand, within Pf1N1B4_343at 407.205 kb on - strand, within Pf1N1B4_343at 407.205 kb on - strand, within Pf1N1B4_343at 407.241 kb on - strand, within Pf1N1B4_343at 407.241 kb on - strand, within Pf1N1B4_343at 407.241 kb on - strand, within Pf1N1B4_343at 407.241 kb on - strand, within Pf1N1B4_343at 407.268 kb on - strand, within Pf1N1B4_343at 407.268 kb on - strand, within Pf1N1B4_343at 407.418 kb on - strand, within Pf1N1B4_343at 407.418 kb on - strand, within Pf1N1B4_343at 407.422 kb on - strand, within Pf1N1B4_343at 407.472 kb on - strand, within Pf1N1B4_343at 407.557 kb on - strand, within Pf1N1B4_343at 407.557 kb on - strand, within Pf1N1B4_343at 407.841 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 6 mM
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404,993 + -1.2
404,993 + +0.4
404,993 + +1.0
404,994 - +0.8
404,994 - -0.4
404,994 - -0.6
404,994 - +2.4
405,029 - -1.2
405,029 - -1.6
405,128 - +1.6
405,128 - -2.6
405,128 - -0.2
405,359 - +2.1
405,443 - Pf1N1B4_341 0.39 +0.8
405,443 - Pf1N1B4_341 0.39 -0.8
405,443 - Pf1N1B4_341 0.39 +1.1
405,448 + Pf1N1B4_341 0.40 +1.0
405,448 + Pf1N1B4_341 0.40 -0.5
405,448 + Pf1N1B4_341 0.40 -0.8
405,449 - Pf1N1B4_341 0.40 -0.2
405,476 + Pf1N1B4_341 0.43 -0.2
405,477 - Pf1N1B4_341 0.43 -0.2
405,574 - Pf1N1B4_341 0.54 +1.3
405,635 - Pf1N1B4_341 0.61 -0.2
405,635 - Pf1N1B4_341 0.61 -3.0
405,726 - Pf1N1B4_341 0.71 +0.4
405,740 - Pf1N1B4_341 0.73 -0.2
405,815 + +1.4
405,815 + Pf1N1B4_341 0.81 -0.2
405,816 - Pf1N1B4_341 0.81 +0.2
405,966 - +1.2
405,985 + -0.3
405,985 + +0.6
406,896 - +0.8
406,943 - -1.2
406,943 - +1.4
407,055 - Pf1N1B4_343 0.17 +0.8
407,088 - -0.8
407,088 - Pf1N1B4_343 0.20 -1.0
407,158 - Pf1N1B4_343 0.27 -1.5
407,205 - Pf1N1B4_343 0.31 -0.5
407,205 - Pf1N1B4_343 0.31 -1.6
407,205 - Pf1N1B4_343 0.31 -0.0
407,205 - Pf1N1B4_343 0.31 -1.5
407,241 - Pf1N1B4_343 0.34 +0.0
407,241 - Pf1N1B4_343 0.34 -0.8
407,241 - Pf1N1B4_343 0.34 -0.8
407,241 - Pf1N1B4_343 0.34 -1.0
407,268 - Pf1N1B4_343 0.37 +0.6
407,268 - Pf1N1B4_343 0.37 -0.5
407,418 - Pf1N1B4_343 0.51 +0.1
407,418 - Pf1N1B4_343 0.51 +0.8
407,422 - Pf1N1B4_343 0.51 +0.1
407,472 - Pf1N1B4_343 0.56 -1.5
407,557 - Pf1N1B4_343 0.64 +0.2
407,557 - Pf1N1B4_343 0.64 -0.8
407,841 - -0.8

Or see this region's nucleotide sequence