Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3351 and Pf1N1B4_3352 are separated by 92 nucleotides Pf1N1B4_3352 and Pf1N1B4_3353 are separated by 230 nucleotides Pf1N1B4_3353 and Pf1N1B4_3354 are separated by 233 nucleotides
Pf1N1B4_3351 - FIG00954302: hypothetical protein, at 3,738,695 to 3,739,072
_3351
Pf1N1B4_3352 - tRNA (guanosine(18)-2'-O)-methyltransferase (EC 2.1.1.34), at 3,739,165 to 3,739,635
_3352
Pf1N1B4_3353 - putative cytoplasmic protein, at 3,739,866 to 3,740,237
_3353
Pf1N1B4_3354 - FIG00954793: hypothetical protein, at 3,740,471 to 3,741,157
_3354
Position (kb)
3739
3740
3741 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3738.911 kb on + strand, within Pf1N1B4_3351 at 3738.912 kb on - strand, within Pf1N1B4_3351 at 3738.912 kb on - strand, within Pf1N1B4_3351 at 3738.956 kb on + strand, within Pf1N1B4_3351 at 3738.956 kb on + strand, within Pf1N1B4_3351 at 3738.956 kb on + strand, within Pf1N1B4_3351 at 3738.956 kb on + strand, within Pf1N1B4_3351 at 3739.045 kb on + strand at 3739.046 kb on - strand at 3739.052 kb on + strand at 3739.052 kb on + strand at 3739.109 kb on + strand at 3739.109 kb on + strand at 3739.109 kb on + strand at 3739.109 kb on + strand at 3739.110 kb on - strand at 3739.110 kb on - strand at 3739.110 kb on - strand at 3739.110 kb on - strand at 3739.113 kb on + strand at 3739.113 kb on + strand at 3739.113 kb on + strand at 3739.114 kb on - strand at 3739.114 kb on - strand at 3739.114 kb on - strand at 3739.114 kb on - strand at 3739.117 kb on + strand at 3739.118 kb on - strand at 3739.118 kb on - strand at 3739.271 kb on + strand, within Pf1N1B4_3352 at 3739.271 kb on + strand, within Pf1N1B4_3352 at 3739.271 kb on + strand, within Pf1N1B4_3352 at 3739.272 kb on - strand, within Pf1N1B4_3352 at 3739.337 kb on + strand, within Pf1N1B4_3352 at 3739.337 kb on + strand, within Pf1N1B4_3352 at 3739.337 kb on + strand, within Pf1N1B4_3352 at 3739.337 kb on + strand at 3739.337 kb on + strand, within Pf1N1B4_3352 at 3739.338 kb on - strand, within Pf1N1B4_3352 at 3739.338 kb on - strand, within Pf1N1B4_3352 at 3739.338 kb on - strand, within Pf1N1B4_3352 at 3739.361 kb on + strand, within Pf1N1B4_3352 at 3739.361 kb on + strand, within Pf1N1B4_3352 at 3739.467 kb on - strand, within Pf1N1B4_3352 at 3739.601 kb on + strand at 3739.601 kb on + strand at 3739.601 kb on + strand at 3739.601 kb on + strand at 3739.601 kb on + strand at 3739.601 kb on + strand at 3739.601 kb on + strand at 3739.601 kb on + strand at 3739.601 kb on + strand at 3739.601 kb on + strand at 3739.601 kb on + strand at 3739.601 kb on + strand at 3739.601 kb on + strand at 3739.602 kb on - strand at 3739.602 kb on - strand at 3739.602 kb on - strand at 3739.602 kb on - strand at 3739.602 kb on - strand at 3739.602 kb on - strand at 3739.602 kb on - strand at 3739.602 kb on - strand at 3739.620 kb on - strand at 3739.668 kb on + strand at 3739.669 kb on - strand at 3739.671 kb on - strand at 3739.671 kb on - strand at 3739.867 kb on + strand at 3739.868 kb on - strand at 3739.937 kb on + strand, within Pf1N1B4_3353 at 3739.937 kb on + strand, within Pf1N1B4_3353 at 3739.960 kb on + strand, within Pf1N1B4_3353 at 3739.960 kb on + strand, within Pf1N1B4_3353 at 3739.960 kb on + strand, within Pf1N1B4_3353 at 3739.960 kb on + strand, within Pf1N1B4_3353 at 3739.961 kb on - strand, within Pf1N1B4_3353 at 3739.974 kb on - strand, within Pf1N1B4_3353 at 3740.003 kb on - strand, within Pf1N1B4_3353 at 3740.003 kb on - strand at 3740.003 kb on - strand, within Pf1N1B4_3353 at 3740.134 kb on + strand, within Pf1N1B4_3353 at 3740.135 kb on - strand at 3740.251 kb on + strand at 3740.251 kb on + strand at 3740.252 kb on - strand at 3740.260 kb on + strand at 3740.261 kb on - strand at 3740.270 kb on + strand at 3740.271 kb on - strand at 3740.271 kb on - strand at 3740.271 kb on - strand at 3740.271 kb on - strand at 3740.284 kb on - strand at 3740.300 kb on + strand at 3740.300 kb on + strand at 3740.300 kb on + strand at 3740.300 kb on + strand at 3740.300 kb on + strand at 3740.301 kb on - strand at 3740.301 kb on - strand at 3740.301 kb on - strand at 3740.301 kb on - strand at 3740.301 kb on - strand at 3740.301 kb on - strand at 3740.301 kb on - strand at 3740.301 kb on - strand at 3740.304 kb on + strand at 3740.304 kb on + strand at 3740.304 kb on + strand at 3740.304 kb on + strand at 3740.304 kb on + strand at 3740.304 kb on + strand at 3740.304 kb on + strand at 3740.304 kb on + strand at 3740.305 kb on - strand at 3740.305 kb on - strand at 3740.305 kb on - strand at 3740.305 kb on - strand at 3740.305 kb on - strand at 3740.305 kb on - strand at 3740.305 kb on - strand at 3740.305 kb on - strand at 3740.308 kb on - strand at 3740.446 kb on + strand at 3740.446 kb on + strand at 3740.446 kb on + strand at 3740.446 kb on + strand at 3740.447 kb on - strand at 3740.447 kb on - strand at 3740.447 kb on - strand at 3740.447 kb on - strand at 3740.447 kb on - strand at 3740.447 kb on - strand at 3740.450 kb on + strand at 3740.450 kb on + strand at 3740.450 kb on + strand at 3740.450 kb on + strand at 3740.450 kb on + strand at 3740.451 kb on - strand at 3740.451 kb on - strand at 3740.451 kb on - strand at 3740.451 kb on - strand at 3740.451 kb on - strand at 3740.451 kb on - strand at 3740.451 kb on - strand at 3740.451 kb on - strand at 3740.451 kb on - strand at 3740.671 kb on - strand, within Pf1N1B4_3354 at 3740.671 kb on - strand at 3740.783 kb on - strand, within Pf1N1B4_3354 at 3740.783 kb on - strand, within Pf1N1B4_3354 at 3741.088 kb on - strand, within Pf1N1B4_3354 at 3741.184 kb on + strand at 3741.184 kb on + strand at 3741.185 kb on - strand at 3741.185 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 3,738,911 + Pf1N1B4_3351 0.57 +0.4 3,738,912 - Pf1N1B4_3351 0.57 +0.2 3,738,912 - Pf1N1B4_3351 0.57 -1.8 3,738,956 + Pf1N1B4_3351 0.69 +1.9 3,738,956 + Pf1N1B4_3351 0.69 +0.9 3,738,956 + Pf1N1B4_3351 0.69 -0.7 3,738,956 + Pf1N1B4_3351 0.69 -0.4 3,739,045 + -1.7 3,739,046 - -1.9 3,739,052 + +0.3 3,739,052 + +0.3 3,739,109 + -0.4 3,739,109 + -1.1 3,739,109 + +0.9 3,739,109 + +0.3 3,739,110 - -0.5 3,739,110 - +1.8 3,739,110 - +0.2 3,739,110 - -0.1 3,739,113 + +0.3 3,739,113 + -0.4 3,739,113 + +0.1 3,739,114 - -0.5 3,739,114 - +0.0 3,739,114 - +0.9 3,739,114 - +0.2 3,739,117 + -0.3 3,739,118 - +0.9 3,739,118 - +0.1 3,739,271 + Pf1N1B4_3352 0.23 +1.6 3,739,271 + Pf1N1B4_3352 0.23 +0.9 3,739,271 + Pf1N1B4_3352 0.23 -0.1 3,739,272 - Pf1N1B4_3352 0.23 -1.7 3,739,337 + Pf1N1B4_3352 0.37 +1.9 3,739,337 + Pf1N1B4_3352 0.37 -0.1 3,739,337 + Pf1N1B4_3352 0.37 -0.4 3,739,337 + -0.7 3,739,337 + Pf1N1B4_3352 0.37 +0.2 3,739,338 - Pf1N1B4_3352 0.37 +1.2 3,739,338 - Pf1N1B4_3352 0.37 -0.8 3,739,338 - Pf1N1B4_3352 0.37 +0.1 3,739,361 + Pf1N1B4_3352 0.42 -1.7 3,739,361 + Pf1N1B4_3352 0.42 +0.9 3,739,467 - Pf1N1B4_3352 0.64 -1.9 3,739,601 + -1.1 3,739,601 + -1.1 3,739,601 + -0.7 3,739,601 + -0.7 3,739,601 + +0.3 3,739,601 + +1.2 3,739,601 + -0.4 3,739,601 + -1.1 3,739,601 + -1.0 3,739,601 + +0.0 3,739,601 + -1.4 3,739,601 + -0.4 3,739,601 + -0.7 3,739,602 - -0.1 3,739,602 - +0.4 3,739,602 - +0.3 3,739,602 - -2.3 3,739,602 - +2.0 3,739,602 - +1.3 3,739,602 - +0.6 3,739,602 - +0.6 3,739,620 - -0.7 3,739,668 + -1.4 3,739,669 - -0.4 3,739,671 - -2.0 3,739,671 - -0.6 3,739,867 + +1.6 3,739,868 - -0.1 3,739,937 + Pf1N1B4_3353 0.19 +0.5 3,739,937 + Pf1N1B4_3353 0.19 +0.5 3,739,960 + Pf1N1B4_3353 0.25 -1.9 3,739,960 + Pf1N1B4_3353 0.25 -1.7 3,739,960 + Pf1N1B4_3353 0.25 -0.1 3,739,960 + Pf1N1B4_3353 0.25 -2.0 3,739,961 - Pf1N1B4_3353 0.26 -1.4 3,739,974 - Pf1N1B4_3353 0.29 -0.1 3,740,003 - Pf1N1B4_3353 0.37 -0.8 3,740,003 - +0.5 3,740,003 - Pf1N1B4_3353 0.37 -1.1 3,740,134 + Pf1N1B4_3353 0.72 -0.6 3,740,135 - -0.1 3,740,251 + -0.9 3,740,251 + +1.0 3,740,252 - +0.0 3,740,260 + -2.3 3,740,261 - -0.6 3,740,270 + -2.0 3,740,271 - +0.9 3,740,271 - -1.4 3,740,271 - +1.8 3,740,271 - +1.1 3,740,284 - +1.1 3,740,300 + +0.3 3,740,300 + +1.0 3,740,300 + -0.9 3,740,300 + +0.2 3,740,300 + -0.4 3,740,301 - +0.7 3,740,301 - -1.9 3,740,301 - -0.4 3,740,301 - +1.9 3,740,301 - +0.9 3,740,301 - -1.1 3,740,301 - -0.3 3,740,301 - +0.5 3,740,304 + +0.9 3,740,304 + +0.9 3,740,304 + +0.5 3,740,304 + +0.2 3,740,304 + -0.3 3,740,304 + -0.9 3,740,304 + -0.1 3,740,304 + +1.9 3,740,305 - +0.3 3,740,305 - -1.7 3,740,305 - +0.9 3,740,305 - +1.5 3,740,305 - +0.6 3,740,305 - -0.1 3,740,305 - +0.3 3,740,305 - +0.6 3,740,308 - -0.9 3,740,446 + +0.2 3,740,446 + +2.9 3,740,446 + +0.9 3,740,446 + +0.9 3,740,447 - +1.9 3,740,447 - +0.6 3,740,447 - +0.7 3,740,447 - -0.1 3,740,447 - +0.4 3,740,447 - +0.8 3,740,450 + -0.5 3,740,450 + +0.9 3,740,450 + +1.2 3,740,450 + -1.3 3,740,450 + +0.2 3,740,451 - -1.6 3,740,451 - -0.1 3,740,451 - +1.9 3,740,451 - +0.1 3,740,451 - -0.5 3,740,451 - -0.7 3,740,451 - +0.0 3,740,451 - -1.7 3,740,451 - -0.1 3,740,671 - Pf1N1B4_3354 0.29 -0.7 3,740,671 - -0.7 3,740,783 - Pf1N1B4_3354 0.45 +1.7 3,740,783 - Pf1N1B4_3354 0.45 -0.1 3,741,088 - Pf1N1B4_3354 0.90 +0.4 3,741,184 + -0.9 3,741,184 + -0.7 3,741,185 - -0.4 3,741,185 - -0.7
Or see this region's nucleotide sequence