Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3327 and Pf1N1B4_3328 are separated by 97 nucleotides Pf1N1B4_3328 and Pf1N1B4_3329 are separated by 31 nucleotides Pf1N1B4_3329 and Pf1N1B4_3330 are separated by 112 nucleotides Pf1N1B4_3330 and Pf1N1B4_3331 are separated by 10 nucleotides
Pf1N1B4_3327 - Exported zinc metalloprotease YfgC precursor, at 3,715,437 to 3,716,870
_3327
Pf1N1B4_3328 - Rhodanese-like domain protein, at 3,716,968 to 3,717,207
_3328
Pf1N1B4_3329 - Putative permease PerM (= YfgO), at 3,717,239 to 3,718,309
_3329
Pf1N1B4_3330 - Thiol peroxidase, Bcp-type (EC 1.11.1.15), at 3,718,422 to 3,718,895
_3330
Pf1N1B4_3331 - Glycine cleavage system transcriptional antiactivator GcvR, at 3,718,906 to 3,719,466
_3331
Position (kb)
3717
3718
3719 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3716.899 kb on - strand at 3716.899 kb on - strand at 3716.899 kb on - strand at 3716.930 kb on + strand at 3716.935 kb on + strand at 3716.981 kb on + strand at 3717.077 kb on + strand, within Pf1N1B4_3328 at 3717.147 kb on + strand, within Pf1N1B4_3328 at 3717.147 kb on + strand, within Pf1N1B4_3328 at 3717.147 kb on + strand, within Pf1N1B4_3328 at 3717.227 kb on + strand at 3717.227 kb on + strand at 3717.234 kb on + strand at 3717.234 kb on + strand at 3717.272 kb on + strand at 3717.325 kb on + strand at 3717.491 kb on - strand, within Pf1N1B4_3329 at 3717.731 kb on + strand, within Pf1N1B4_3329 at 3717.731 kb on + strand at 3717.732 kb on - strand, within Pf1N1B4_3329 at 3717.732 kb on - strand, within Pf1N1B4_3329 at 3717.806 kb on + strand at 3717.872 kb on + strand, within Pf1N1B4_3329 at 3717.872 kb on + strand, within Pf1N1B4_3329 at 3717.872 kb on + strand, within Pf1N1B4_3329 at 3717.872 kb on + strand, within Pf1N1B4_3329 at 3717.872 kb on + strand, within Pf1N1B4_3329 at 3717.872 kb on + strand, within Pf1N1B4_3329 at 3717.872 kb on + strand, within Pf1N1B4_3329 at 3717.872 kb on + strand, within Pf1N1B4_3329 at 3717.872 kb on + strand, within Pf1N1B4_3329 at 3717.872 kb on + strand, within Pf1N1B4_3329 at 3717.873 kb on - strand, within Pf1N1B4_3329 at 3717.873 kb on - strand, within Pf1N1B4_3329 at 3717.874 kb on + strand, within Pf1N1B4_3329 at 3717.874 kb on + strand, within Pf1N1B4_3329 at 3717.875 kb on - strand, within Pf1N1B4_3329 at 3717.875 kb on - strand, within Pf1N1B4_3329 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand at 3717.920 kb on + strand at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.950 kb on + strand, within Pf1N1B4_3329 at 3717.950 kb on + strand, within Pf1N1B4_3329 at 3717.951 kb on - strand, within Pf1N1B4_3329 at 3718.076 kb on + strand, within Pf1N1B4_3329 at 3718.076 kb on + strand, within Pf1N1B4_3329 at 3718.221 kb on + strand at 3718.307 kb on + strand at 3718.307 kb on + strand at 3718.352 kb on - strand at 3718.375 kb on - strand at 3718.375 kb on - strand at 3718.552 kb on + strand, within Pf1N1B4_3330 at 3718.552 kb on + strand, within Pf1N1B4_3330 at 3718.552 kb on + strand, within Pf1N1B4_3330 at 3718.552 kb on + strand, within Pf1N1B4_3330 at 3718.553 kb on - strand, within Pf1N1B4_3330 at 3718.553 kb on - strand, within Pf1N1B4_3330 at 3718.553 kb on - strand, within Pf1N1B4_3330 at 3718.706 kb on + strand, within Pf1N1B4_3330 at 3718.719 kb on - strand, within Pf1N1B4_3330 at 3718.787 kb on - strand, within Pf1N1B4_3330 at 3718.792 kb on + strand, within Pf1N1B4_3330 at 3718.792 kb on + strand, within Pf1N1B4_3330 at 3718.792 kb on + strand, within Pf1N1B4_3330 at 3718.792 kb on + strand, within Pf1N1B4_3330 at 3718.793 kb on - strand at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.793 kb on - strand at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.907 kb on + strand at 3718.907 kb on + strand at 3718.908 kb on - strand at 3718.908 kb on - strand at 3718.908 kb on - strand at 3719.069 kb on + strand, within Pf1N1B4_3331 at 3719.069 kb on + strand, within Pf1N1B4_3331 at 3719.070 kb on - strand, within Pf1N1B4_3331 at 3719.070 kb on - strand, within Pf1N1B4_3331 at 3719.070 kb on - strand, within Pf1N1B4_3331 at 3719.165 kb on + strand, within Pf1N1B4_3331 at 3719.166 kb on - strand, within Pf1N1B4_3331 at 3719.166 kb on - strand, within Pf1N1B4_3331 at 3719.166 kb on - strand, within Pf1N1B4_3331 at 3719.174 kb on + strand, within Pf1N1B4_3331 at 3719.175 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 3,716,899 - -0.1 3,716,899 - +0.3 3,716,899 - -1.2 3,716,930 + +0.9 3,716,935 + +0.9 3,716,981 + +1.2 3,717,077 + Pf1N1B4_3328 0.45 +0.5 3,717,147 + Pf1N1B4_3328 0.75 +0.6 3,717,147 + Pf1N1B4_3328 0.75 +2.2 3,717,147 + Pf1N1B4_3328 0.75 +1.1 3,717,227 + -3.1 3,717,227 + -1.4 3,717,234 + -0.1 3,717,234 + -0.5 3,717,272 + -0.7 3,717,325 + -0.7 3,717,491 - Pf1N1B4_3329 0.24 +0.3 3,717,731 + Pf1N1B4_3329 0.46 -2.3 3,717,731 + +0.9 3,717,732 - Pf1N1B4_3329 0.46 +0.2 3,717,732 - Pf1N1B4_3329 0.46 -0.9 3,717,806 + -0.1 3,717,872 + Pf1N1B4_3329 0.59 -0.5 3,717,872 + Pf1N1B4_3329 0.59 -1.3 3,717,872 + Pf1N1B4_3329 0.59 -1.4 3,717,872 + Pf1N1B4_3329 0.59 -0.3 3,717,872 + Pf1N1B4_3329 0.59 +0.5 3,717,872 + Pf1N1B4_3329 0.59 +0.9 3,717,872 + Pf1N1B4_3329 0.59 -1.4 3,717,872 + Pf1N1B4_3329 0.59 -0.3 3,717,872 + Pf1N1B4_3329 0.59 +0.1 3,717,872 + Pf1N1B4_3329 0.59 -2.1 3,717,873 - Pf1N1B4_3329 0.59 -0.7 3,717,873 - Pf1N1B4_3329 0.59 +0.5 3,717,874 + Pf1N1B4_3329 0.59 -0.1 3,717,874 + Pf1N1B4_3329 0.59 -1.4 3,717,875 - Pf1N1B4_3329 0.59 +0.3 3,717,875 - Pf1N1B4_3329 0.59 -0.7 3,717,920 + Pf1N1B4_3329 0.64 -2.6 3,717,920 + Pf1N1B4_3329 0.64 -1.7 3,717,920 + +0.9 3,717,920 + +0.9 3,717,920 + Pf1N1B4_3329 0.64 +0.3 3,717,920 + Pf1N1B4_3329 0.64 -2.3 3,717,920 + Pf1N1B4_3329 0.64 -0.8 3,717,920 + Pf1N1B4_3329 0.64 -1.3 3,717,950 + Pf1N1B4_3329 0.66 -0.7 3,717,950 + Pf1N1B4_3329 0.66 -0.9 3,717,951 - Pf1N1B4_3329 0.66 -2.3 3,718,076 + Pf1N1B4_3329 0.78 +0.8 3,718,076 + Pf1N1B4_3329 0.78 -1.3 3,718,221 + -0.7 3,718,307 + -1.1 3,718,307 + -1.4 3,718,352 - -0.7 3,718,375 - +2.2 3,718,375 - +1.6 3,718,552 + Pf1N1B4_3330 0.27 -0.1 3,718,552 + Pf1N1B4_3330 0.27 +0.3 3,718,552 + Pf1N1B4_3330 0.27 +0.3 3,718,552 + Pf1N1B4_3330 0.27 -1.9 3,718,553 - Pf1N1B4_3330 0.28 -0.1 3,718,553 - Pf1N1B4_3330 0.28 -1.2 3,718,553 - Pf1N1B4_3330 0.28 -0.8 3,718,706 + Pf1N1B4_3330 0.60 -0.7 3,718,719 - Pf1N1B4_3330 0.63 -0.4 3,718,787 - Pf1N1B4_3330 0.77 -1.9 3,718,792 + Pf1N1B4_3330 0.78 -1.1 3,718,792 + Pf1N1B4_3330 0.78 +3.5 3,718,792 + Pf1N1B4_3330 0.78 -1.9 3,718,792 + Pf1N1B4_3330 0.78 +1.9 3,718,793 - -1.4 3,718,793 - Pf1N1B4_3330 0.78 +0.3 3,718,793 - Pf1N1B4_3330 0.78 +0.1 3,718,793 - +1.5 3,718,793 - Pf1N1B4_3330 0.78 -0.7 3,718,793 - Pf1N1B4_3330 0.78 -0.3 3,718,793 - Pf1N1B4_3330 0.78 -0.4 3,718,907 + -0.1 3,718,907 + -1.7 3,718,908 - -0.7 3,718,908 - +0.9 3,718,908 - -0.7 3,719,069 + Pf1N1B4_3331 0.29 -0.1 3,719,069 + Pf1N1B4_3331 0.29 +0.3 3,719,070 - Pf1N1B4_3331 0.29 -1.1 3,719,070 - Pf1N1B4_3331 0.29 -2.1 3,719,070 - Pf1N1B4_3331 0.29 +1.2 3,719,165 + Pf1N1B4_3331 0.46 +1.3 3,719,166 - Pf1N1B4_3331 0.46 +0.1 3,719,166 - Pf1N1B4_3331 0.46 +1.6 3,719,166 - Pf1N1B4_3331 0.46 -1.4 3,719,174 + Pf1N1B4_3331 0.48 -1.4 3,719,175 - +0.3
Or see this region's nucleotide sequence