Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3209 and Pf1N1B4_3210 overlap by 4 nucleotides Pf1N1B4_3210 and Pf1N1B4_3211 are separated by 25 nucleotides Pf1N1B4_3211 and Pf1N1B4_3212 are separated by 116 nucleotides Pf1N1B4_3212 and Pf1N1B4_3213 overlap by 7 nucleotides
Pf1N1B4_3209 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,592,424 to 3,593,182
_3209
Pf1N1B4_3210 - Uncharacterized protein conserved in bacteria, NMA0228-like, at 3,593,179 to 3,594,033
_3210
Pf1N1B4_3211 - Putative signal peptide protein, at 3,594,059 to 3,594,364
_3211
Pf1N1B4_3212 - hypothetical protein, at 3,594,481 to 3,595,173
_3212
Pf1N1B4_3213 - hypothetical protein, at 3,595,167 to 3,595,376
_3213
Position (kb)
3594
3595 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3593.062 kb on + strand, within Pf1N1B4_3209 at 3593.063 kb on - strand, within Pf1N1B4_3209 at 3593.063 kb on - strand, within Pf1N1B4_3209 at 3593.073 kb on + strand, within Pf1N1B4_3209 at 3593.073 kb on + strand, within Pf1N1B4_3209 at 3593.255 kb on - strand at 3593.315 kb on + strand, within Pf1N1B4_3210 at 3593.315 kb on + strand, within Pf1N1B4_3210 at 3593.388 kb on + strand, within Pf1N1B4_3210 at 3593.389 kb on - strand, within Pf1N1B4_3210 at 3593.389 kb on - strand, within Pf1N1B4_3210 at 3593.451 kb on - strand, within Pf1N1B4_3210 at 3593.451 kb on - strand, within Pf1N1B4_3210 at 3593.489 kb on + strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.588 kb on + strand, within Pf1N1B4_3210 at 3593.588 kb on + strand, within Pf1N1B4_3210 at 3593.589 kb on - strand, within Pf1N1B4_3210 at 3593.589 kb on - strand, within Pf1N1B4_3210 at 3593.589 kb on - strand, within Pf1N1B4_3210 at 3593.646 kb on + strand, within Pf1N1B4_3210 at 3593.647 kb on - strand, within Pf1N1B4_3210 at 3593.682 kb on - strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.827 kb on - strand, within Pf1N1B4_3210 at 3593.858 kb on + strand, within Pf1N1B4_3210 at 3593.858 kb on + strand, within Pf1N1B4_3210 at 3593.858 kb on + strand, within Pf1N1B4_3210 at 3593.912 kb on + strand, within Pf1N1B4_3210 at 3593.912 kb on + strand at 3593.912 kb on + strand at 3593.912 kb on + strand, within Pf1N1B4_3210 at 3593.913 kb on - strand, within Pf1N1B4_3210 at 3593.913 kb on - strand, within Pf1N1B4_3210 at 3593.913 kb on - strand, within Pf1N1B4_3210 at 3594.075 kb on + strand at 3594.075 kb on + strand at 3594.075 kb on + strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.196 kb on + strand, within Pf1N1B4_3211 at 3594.196 kb on + strand, within Pf1N1B4_3211 at 3594.196 kb on + strand, within Pf1N1B4_3211 at 3594.196 kb on + strand, within Pf1N1B4_3211 at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.197 kb on - strand at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.199 kb on + strand, within Pf1N1B4_3211 at 3594.413 kb on + strand at 3594.413 kb on + strand at 3594.414 kb on - strand at 3594.498 kb on + strand at 3594.593 kb on + strand at 3594.594 kb on - strand, within Pf1N1B4_3212 at 3594.594 kb on - strand, within Pf1N1B4_3212 at 3594.620 kb on + strand, within Pf1N1B4_3212 at 3594.696 kb on - strand, within Pf1N1B4_3212 at 3594.785 kb on + strand, within Pf1N1B4_3212 at 3594.786 kb on - strand, within Pf1N1B4_3212 at 3594.870 kb on - strand, within Pf1N1B4_3212 at 3594.870 kb on - strand, within Pf1N1B4_3212 at 3595.148 kb on - strand at 3595.227 kb on - strand, within Pf1N1B4_3213
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 3,593,062 + Pf1N1B4_3209 0.84 -1.5 3,593,063 - Pf1N1B4_3209 0.84 -1.0 3,593,063 - Pf1N1B4_3209 0.84 -0.2 3,593,073 + Pf1N1B4_3209 0.86 -0.3 3,593,073 + Pf1N1B4_3209 0.86 +1.0 3,593,255 - -1.2 3,593,315 + Pf1N1B4_3210 0.16 -2.2 3,593,315 + Pf1N1B4_3210 0.16 +0.2 3,593,388 + Pf1N1B4_3210 0.24 -1.8 3,593,389 - Pf1N1B4_3210 0.25 +0.4 3,593,389 - Pf1N1B4_3210 0.25 +0.0 3,593,451 - Pf1N1B4_3210 0.32 -0.3 3,593,451 - Pf1N1B4_3210 0.32 -1.5 3,593,489 + Pf1N1B4_3210 0.36 -1.3 3,593,490 - Pf1N1B4_3210 0.36 +3.0 3,593,490 - Pf1N1B4_3210 0.36 -1.3 3,593,490 - Pf1N1B4_3210 0.36 +2.3 3,593,490 - Pf1N1B4_3210 0.36 -0.8 3,593,588 + Pf1N1B4_3210 0.48 +1.4 3,593,588 + Pf1N1B4_3210 0.48 -0.7 3,593,589 - Pf1N1B4_3210 0.48 +1.0 3,593,589 - Pf1N1B4_3210 0.48 +0.0 3,593,589 - Pf1N1B4_3210 0.48 +0.2 3,593,646 + Pf1N1B4_3210 0.55 -0.0 3,593,647 - Pf1N1B4_3210 0.55 +0.6 3,593,682 - Pf1N1B4_3210 0.59 +0.0 3,593,826 + Pf1N1B4_3210 0.76 -0.1 3,593,826 + Pf1N1B4_3210 0.76 -1.2 3,593,826 + Pf1N1B4_3210 0.76 -0.5 3,593,826 + Pf1N1B4_3210 0.76 -1.0 3,593,827 - Pf1N1B4_3210 0.76 -0.4 3,593,858 + Pf1N1B4_3210 0.79 -1.0 3,593,858 + Pf1N1B4_3210 0.79 +0.4 3,593,858 + Pf1N1B4_3210 0.79 -1.0 3,593,912 + Pf1N1B4_3210 0.86 +0.0 3,593,912 + +0.0 3,593,912 + +0.0 3,593,912 + Pf1N1B4_3210 0.86 +0.7 3,593,913 - Pf1N1B4_3210 0.86 -1.2 3,593,913 - Pf1N1B4_3210 0.86 -0.5 3,593,913 - Pf1N1B4_3210 0.86 +1.0 3,594,075 + -0.3 3,594,075 + +0.6 3,594,075 + -1.6 3,594,076 - -1.2 3,594,076 - +0.0 3,594,076 - -1.6 3,594,076 - -0.6 3,594,076 - -0.6 3,594,076 - -2.2 3,594,196 + Pf1N1B4_3211 0.45 -1.1 3,594,196 + Pf1N1B4_3211 0.45 +0.8 3,594,196 + Pf1N1B4_3211 0.45 -3.0 3,594,196 + Pf1N1B4_3211 0.45 +0.0 3,594,197 - Pf1N1B4_3211 0.45 -0.8 3,594,197 - Pf1N1B4_3211 0.45 -0.4 3,594,197 - Pf1N1B4_3211 0.45 -0.4 3,594,197 - Pf1N1B4_3211 0.45 -0.2 3,594,197 - +1.0 3,594,197 - Pf1N1B4_3211 0.45 -0.6 3,594,199 + Pf1N1B4_3211 0.46 -2.1 3,594,413 + +1.3 3,594,413 + -1.6 3,594,414 - -2.7 3,594,498 + +1.0 3,594,593 + +0.6 3,594,594 - Pf1N1B4_3212 0.16 +0.2 3,594,594 - Pf1N1B4_3212 0.16 -0.2 3,594,620 + Pf1N1B4_3212 0.20 -1.3 3,594,696 - Pf1N1B4_3212 0.31 -0.1 3,594,785 + Pf1N1B4_3212 0.44 -1.0 3,594,786 - Pf1N1B4_3212 0.44 -0.4 3,594,870 - Pf1N1B4_3212 0.56 +1.0 3,594,870 - Pf1N1B4_3212 0.56 +0.2 3,595,148 - -1.0 3,595,227 - Pf1N1B4_3213 0.29 -0.2
Or see this region's nucleotide sequence