Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3128 and Pf1N1B4_3129 are separated by 135 nucleotides Pf1N1B4_3129 and Pf1N1B4_3130 overlap by 1 nucleotides Pf1N1B4_3130 and Pf1N1B4_3131 overlap by 11 nucleotides
Pf1N1B4_3128 - FIG003269: Prophage tail protein, at 3,513,921 to 3,514,964
_3128
Pf1N1B4_3129 - Prophage baseplate assembly protein V, at 3,515,100 to 3,515,609
_3129
Pf1N1B4_3130 - Bacteriophage protein GP46, at 3,515,609 to 3,516,007
_3130
Pf1N1B4_3131 - Phage FluMu protein gp47, at 3,515,997 to 3,517,040
_3131
Position (kb)
3515
3516 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3514.134 kb on + strand, within Pf1N1B4_3128 at 3514.135 kb on - strand, within Pf1N1B4_3128 at 3514.159 kb on - strand, within Pf1N1B4_3128 at 3514.159 kb on - strand, within Pf1N1B4_3128 at 3514.159 kb on - strand, within Pf1N1B4_3128 at 3514.296 kb on + strand, within Pf1N1B4_3128 at 3514.296 kb on + strand, within Pf1N1B4_3128 at 3514.296 kb on + strand, within Pf1N1B4_3128 at 3514.296 kb on + strand, within Pf1N1B4_3128 at 3514.296 kb on + strand, within Pf1N1B4_3128 at 3514.296 kb on + strand, within Pf1N1B4_3128 at 3514.296 kb on + strand, within Pf1N1B4_3128 at 3514.297 kb on - strand, within Pf1N1B4_3128 at 3514.297 kb on - strand at 3514.297 kb on - strand at 3514.297 kb on - strand, within Pf1N1B4_3128 at 3514.297 kb on - strand, within Pf1N1B4_3128 at 3514.297 kb on - strand, within Pf1N1B4_3128 at 3514.372 kb on + strand, within Pf1N1B4_3128 at 3514.372 kb on + strand, within Pf1N1B4_3128 at 3514.373 kb on - strand, within Pf1N1B4_3128 at 3514.373 kb on - strand, within Pf1N1B4_3128 at 3514.373 kb on - strand, within Pf1N1B4_3128 at 3514.373 kb on - strand, within Pf1N1B4_3128 at 3514.373 kb on - strand, within Pf1N1B4_3128 at 3514.502 kb on + strand, within Pf1N1B4_3128 at 3514.502 kb on + strand, within Pf1N1B4_3128 at 3514.502 kb on + strand, within Pf1N1B4_3128 at 3514.530 kb on + strand, within Pf1N1B4_3128 at 3514.531 kb on - strand, within Pf1N1B4_3128 at 3514.638 kb on + strand, within Pf1N1B4_3128 at 3514.638 kb on + strand, within Pf1N1B4_3128 at 3514.639 kb on - strand, within Pf1N1B4_3128 at 3514.639 kb on - strand, within Pf1N1B4_3128 at 3514.639 kb on - strand, within Pf1N1B4_3128 at 3514.639 kb on - strand, within Pf1N1B4_3128 at 3514.639 kb on - strand, within Pf1N1B4_3128 at 3514.755 kb on + strand, within Pf1N1B4_3128 at 3514.755 kb on + strand, within Pf1N1B4_3128 at 3514.755 kb on + strand, within Pf1N1B4_3128 at 3514.756 kb on - strand, within Pf1N1B4_3128 at 3514.756 kb on - strand, within Pf1N1B4_3128 at 3514.756 kb on - strand, within Pf1N1B4_3128 at 3514.872 kb on + strand at 3514.872 kb on + strand at 3514.873 kb on - strand at 3514.873 kb on - strand at 3514.873 kb on - strand at 3514.926 kb on - strand at 3515.030 kb on + strand at 3515.030 kb on + strand at 3515.030 kb on + strand at 3515.031 kb on - strand at 3515.038 kb on - strand at 3515.107 kb on + strand at 3515.108 kb on - strand at 3515.371 kb on - strand, within Pf1N1B4_3129 at 3515.371 kb on - strand, within Pf1N1B4_3129 at 3515.371 kb on - strand at 3515.543 kb on - strand, within Pf1N1B4_3129 at 3515.743 kb on + strand, within Pf1N1B4_3130 at 3515.831 kb on + strand, within Pf1N1B4_3130 at 3515.831 kb on + strand, within Pf1N1B4_3130 at 3515.832 kb on - strand, within Pf1N1B4_3130 at 3515.996 kb on + strand at 3515.996 kb on + strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3516.324 kb on + strand, within Pf1N1B4_3131 at 3516.324 kb on + strand, within Pf1N1B4_3131 at 3516.324 kb on + strand, within Pf1N1B4_3131 at 3516.325 kb on - strand, within Pf1N1B4_3131 at 3516.325 kb on - strand, within Pf1N1B4_3131 at 3516.465 kb on - strand, within Pf1N1B4_3131 at 3516.465 kb on - strand, within Pf1N1B4_3131 at 3516.585 kb on + strand, within Pf1N1B4_3131 at 3516.585 kb on + strand, within Pf1N1B4_3131 at 3516.586 kb on - strand, within Pf1N1B4_3131
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 3,514,134 + Pf1N1B4_3128 0.20 +0.6 3,514,135 - Pf1N1B4_3128 0.20 +0.3 3,514,159 - Pf1N1B4_3128 0.23 -0.6 3,514,159 - Pf1N1B4_3128 0.23 +0.6 3,514,159 - Pf1N1B4_3128 0.23 -2.2 3,514,296 + Pf1N1B4_3128 0.36 -0.3 3,514,296 + Pf1N1B4_3128 0.36 -0.4 3,514,296 + Pf1N1B4_3128 0.36 +0.5 3,514,296 + Pf1N1B4_3128 0.36 +1.4 3,514,296 + Pf1N1B4_3128 0.36 +0.2 3,514,296 + Pf1N1B4_3128 0.36 +0.3 3,514,296 + Pf1N1B4_3128 0.36 -0.7 3,514,297 - Pf1N1B4_3128 0.36 +0.5 3,514,297 - +1.1 3,514,297 - +0.7 3,514,297 - Pf1N1B4_3128 0.36 +0.7 3,514,297 - Pf1N1B4_3128 0.36 -0.7 3,514,297 - Pf1N1B4_3128 0.36 -0.9 3,514,372 + Pf1N1B4_3128 0.43 -0.5 3,514,372 + Pf1N1B4_3128 0.43 +1.5 3,514,373 - Pf1N1B4_3128 0.43 -1.1 3,514,373 - Pf1N1B4_3128 0.43 +0.1 3,514,373 - Pf1N1B4_3128 0.43 -0.2 3,514,373 - Pf1N1B4_3128 0.43 -1.1 3,514,373 - Pf1N1B4_3128 0.43 +0.7 3,514,502 + Pf1N1B4_3128 0.56 +0.6 3,514,502 + Pf1N1B4_3128 0.56 -1.9 3,514,502 + Pf1N1B4_3128 0.56 -0.5 3,514,530 + Pf1N1B4_3128 0.58 -0.5 3,514,531 - Pf1N1B4_3128 0.58 -0.4 3,514,638 + Pf1N1B4_3128 0.69 +0.5 3,514,638 + Pf1N1B4_3128 0.69 -0.5 3,514,639 - Pf1N1B4_3128 0.69 -0.8 3,514,639 - Pf1N1B4_3128 0.69 +0.3 3,514,639 - Pf1N1B4_3128 0.69 -0.5 3,514,639 - Pf1N1B4_3128 0.69 +0.4 3,514,639 - Pf1N1B4_3128 0.69 +0.3 3,514,755 + Pf1N1B4_3128 0.80 -1.4 3,514,755 + Pf1N1B4_3128 0.80 -0.5 3,514,755 + Pf1N1B4_3128 0.80 +0.8 3,514,756 - Pf1N1B4_3128 0.80 -0.1 3,514,756 - Pf1N1B4_3128 0.80 -1.5 3,514,756 - Pf1N1B4_3128 0.80 -0.5 3,514,872 + -1.7 3,514,872 + -1.5 3,514,873 - +0.1 3,514,873 - -0.7 3,514,873 - +0.1 3,514,926 - -1.5 3,515,030 + +1.1 3,515,030 + -1.5 3,515,030 + +1.1 3,515,031 - -0.1 3,515,038 - -0.9 3,515,107 + -0.9 3,515,108 - -0.4 3,515,371 - Pf1N1B4_3129 0.53 -1.2 3,515,371 - Pf1N1B4_3129 0.53 +0.1 3,515,371 - +1.1 3,515,543 - Pf1N1B4_3129 0.87 -0.4 3,515,743 + Pf1N1B4_3130 0.34 -1.1 3,515,831 + Pf1N1B4_3130 0.56 -0.9 3,515,831 + Pf1N1B4_3130 0.56 +0.2 3,515,832 - Pf1N1B4_3130 0.56 -0.6 3,515,996 + -1.2 3,515,996 + -0.4 3,515,997 - +0.5 3,515,997 - -0.9 3,515,997 - -0.6 3,515,997 - -0.7 3,515,997 - +1.7 3,515,997 - -0.2 3,515,997 - +0.9 3,515,997 - +0.1 3,516,324 + Pf1N1B4_3131 0.31 +0.3 3,516,324 + Pf1N1B4_3131 0.31 +2.1 3,516,324 + Pf1N1B4_3131 0.31 +0.7 3,516,325 - Pf1N1B4_3131 0.31 -0.3 3,516,325 - Pf1N1B4_3131 0.31 +1.1 3,516,465 - Pf1N1B4_3131 0.45 +0.1 3,516,465 - Pf1N1B4_3131 0.45 -0.5 3,516,585 + Pf1N1B4_3131 0.56 +0.9 3,516,585 + Pf1N1B4_3131 0.56 -0.5 3,516,586 - Pf1N1B4_3131 0.56 +0.7
Or see this region's nucleotide sequence