Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2866 and Pf1N1B4_2867 overlap by 4 nucleotides Pf1N1B4_2867 and Pf1N1B4_2868 overlap by 1 nucleotides Pf1N1B4_2868 and Pf1N1B4_2869 are separated by 21 nucleotides
Pf1N1B4_2866 - Periplasmic hemin-binding protein, at 3,213,775 to 3,214,647
_2866
Pf1N1B4_2867 - Hemin ABC transporter, permease protein, at 3,214,644 to 3,215,681
_2867
Pf1N1B4_2868 - ABC-type hemin transport system, ATPase component, at 3,215,681 to 3,216,448
_2868
Pf1N1B4_2869 - lipoprotein, putative, at 3,216,470 to 3,217,336
_2869
Position (kb)
3214
3215
3216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3213.645 kb on + strand at 3213.645 kb on + strand at 3213.645 kb on + strand at 3213.645 kb on + strand at 3213.645 kb on + strand at 3213.645 kb on + strand at 3213.645 kb on + strand at 3213.645 kb on + strand at 3213.645 kb on + strand at 3213.645 kb on + strand at 3213.645 kb on + strand at 3213.646 kb on - strand at 3213.646 kb on - strand at 3213.646 kb on - strand at 3213.646 kb on - strand at 3213.646 kb on - strand at 3213.646 kb on - strand at 3213.646 kb on - strand at 3213.646 kb on - strand at 3213.678 kb on + strand at 3213.679 kb on - strand at 3213.724 kb on + strand at 3213.724 kb on + strand at 3213.725 kb on - strand at 3213.725 kb on - strand at 3213.761 kb on - strand at 3213.774 kb on + strand at 3213.775 kb on - strand at 3213.775 kb on - strand at 3214.207 kb on + strand, within Pf1N1B4_2866 at 3214.207 kb on + strand, within Pf1N1B4_2866 at 3214.208 kb on - strand, within Pf1N1B4_2866 at 3214.208 kb on - strand at 3214.277 kb on + strand, within Pf1N1B4_2866 at 3214.390 kb on + strand, within Pf1N1B4_2866 at 3214.406 kb on + strand, within Pf1N1B4_2866 at 3214.406 kb on + strand at 3214.442 kb on + strand, within Pf1N1B4_2866 at 3214.442 kb on + strand, within Pf1N1B4_2866 at 3214.442 kb on + strand, within Pf1N1B4_2866 at 3214.442 kb on + strand, within Pf1N1B4_2866 at 3214.443 kb on - strand, within Pf1N1B4_2866 at 3214.625 kb on - strand at 3214.754 kb on + strand, within Pf1N1B4_2867 at 3214.912 kb on + strand, within Pf1N1B4_2867 at 3214.912 kb on + strand, within Pf1N1B4_2867 at 3214.912 kb on + strand, within Pf1N1B4_2867 at 3214.913 kb on - strand, within Pf1N1B4_2867 at 3215.074 kb on + strand, within Pf1N1B4_2867 at 3215.074 kb on + strand, within Pf1N1B4_2867 at 3215.088 kb on + strand, within Pf1N1B4_2867 at 3215.190 kb on + strand, within Pf1N1B4_2867 at 3215.190 kb on + strand, within Pf1N1B4_2867 at 3215.190 kb on + strand, within Pf1N1B4_2867 at 3215.259 kb on + strand, within Pf1N1B4_2867 at 3215.260 kb on - strand, within Pf1N1B4_2867 at 3215.284 kb on + strand, within Pf1N1B4_2867 at 3215.656 kb on - strand at 3215.659 kb on + strand at 3215.659 kb on + strand at 3215.659 kb on + strand at 3215.659 kb on + strand at 3215.659 kb on + strand at 3215.659 kb on + strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3216.071 kb on + strand, within Pf1N1B4_2868 at 3216.072 kb on - strand, within Pf1N1B4_2868 at 3216.291 kb on - strand, within Pf1N1B4_2868 at 3216.303 kb on + strand, within Pf1N1B4_2868 at 3216.304 kb on - strand, within Pf1N1B4_2868 at 3216.306 kb on + strand, within Pf1N1B4_2868 at 3216.306 kb on + strand, within Pf1N1B4_2868 at 3216.306 kb on + strand, within Pf1N1B4_2868 at 3216.307 kb on - strand, within Pf1N1B4_2868 at 3216.307 kb on - strand, within Pf1N1B4_2868 at 3216.307 kb on - strand, within Pf1N1B4_2868 at 3216.340 kb on + strand, within Pf1N1B4_2868 at 3216.397 kb on - strand at 3216.397 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 3,213,645 + -1.7 3,213,645 + -1.9 3,213,645 + +1.5 3,213,645 + -1.6 3,213,645 + -0.8 3,213,645 + -0.7 3,213,645 + +0.8 3,213,645 + -1.6 3,213,645 + -0.3 3,213,645 + -0.7 3,213,645 + +3.6 3,213,646 - +0.5 3,213,646 - +1.3 3,213,646 - +0.7 3,213,646 - +0.7 3,213,646 - -0.3 3,213,646 - +0.2 3,213,646 - -0.5 3,213,646 - +0.4 3,213,678 + -0.6 3,213,679 - -1.7 3,213,724 + -0.4 3,213,724 + +2.8 3,213,725 - +2.1 3,213,725 - +1.1 3,213,761 - -1.3 3,213,774 + -0.7 3,213,775 - +0.7 3,213,775 - +0.3 3,214,207 + Pf1N1B4_2866 0.49 -0.9 3,214,207 + Pf1N1B4_2866 0.49 -0.1 3,214,208 - Pf1N1B4_2866 0.50 +0.2 3,214,208 - -0.8 3,214,277 + Pf1N1B4_2866 0.58 +1.2 3,214,390 + Pf1N1B4_2866 0.70 +1.6 3,214,406 + Pf1N1B4_2866 0.72 -0.8 3,214,406 + +0.2 3,214,442 + Pf1N1B4_2866 0.76 -2.1 3,214,442 + Pf1N1B4_2866 0.76 -0.6 3,214,442 + Pf1N1B4_2866 0.76 -1.9 3,214,442 + Pf1N1B4_2866 0.76 -0.3 3,214,443 - Pf1N1B4_2866 0.77 +1.6 3,214,625 - +0.9 3,214,754 + Pf1N1B4_2867 0.11 -1.1 3,214,912 + Pf1N1B4_2867 0.26 -1.1 3,214,912 + Pf1N1B4_2867 0.26 -0.6 3,214,912 + Pf1N1B4_2867 0.26 +0.4 3,214,913 - Pf1N1B4_2867 0.26 -0.8 3,215,074 + Pf1N1B4_2867 0.41 -1.3 3,215,074 + Pf1N1B4_2867 0.41 +1.2 3,215,088 + Pf1N1B4_2867 0.43 -0.4 3,215,190 + Pf1N1B4_2867 0.53 -1.2 3,215,190 + Pf1N1B4_2867 0.53 -1.9 3,215,190 + Pf1N1B4_2867 0.53 -0.9 3,215,259 + Pf1N1B4_2867 0.59 -0.1 3,215,260 - Pf1N1B4_2867 0.59 -2.9 3,215,284 + Pf1N1B4_2867 0.62 -0.1 3,215,656 - +1.4 3,215,659 + -1.7 3,215,659 + -1.7 3,215,659 + +1.1 3,215,659 + +0.8 3,215,659 + -2.6 3,215,659 + -0.7 3,215,660 - -0.7 3,215,660 - -0.3 3,215,660 - -0.1 3,215,660 - -0.3 3,215,660 - +0.5 3,215,660 - -0.9 3,215,660 - -0.4 3,215,660 - +0.3 3,216,071 + Pf1N1B4_2868 0.51 +0.5 3,216,072 - Pf1N1B4_2868 0.51 +0.9 3,216,291 - Pf1N1B4_2868 0.79 -1.7 3,216,303 + Pf1N1B4_2868 0.81 +0.7 3,216,304 - Pf1N1B4_2868 0.81 -0.5 3,216,306 + Pf1N1B4_2868 0.81 +0.4 3,216,306 + Pf1N1B4_2868 0.81 -1.6 3,216,306 + Pf1N1B4_2868 0.81 +0.1 3,216,307 - Pf1N1B4_2868 0.82 +1.3 3,216,307 - Pf1N1B4_2868 0.82 +0.6 3,216,307 - Pf1N1B4_2868 0.82 +0.3 3,216,340 + Pf1N1B4_2868 0.86 +1.3 3,216,397 - +1.0 3,216,397 - -0.6
Or see this region's nucleotide sequence