Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2676 and Pf1N1B4_2677 overlap by 4 nucleotides Pf1N1B4_2677 and Pf1N1B4_2678 overlap by 8 nucleotides Pf1N1B4_2678 and Pf1N1B4_2679 are separated by 135 nucleotides
Pf1N1B4_2676 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (EC 6.3.2.-), at 3,014,511 to 3,015,860
_2676
Pf1N1B4_2677 - 3-polyprenyl-4-hydroxybenzoate carboxy-lyase UbiX (EC 4.1.1.-), at 3,015,857 to 3,016,489
_2677
Pf1N1B4_2678 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,016,482 to 3,016,766
_2678
Pf1N1B4_2679 - Nucleoside-diphosphate-sugar epimerases, at 3,016,902 to 3,017,543
_2679
Position (kb)
3015
3016
3017 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3015.489 kb on + strand, within Pf1N1B4_2676 at 3015.489 kb on + strand at 3016.487 kb on + strand at 3016.487 kb on + strand at 3016.487 kb on + strand at 3016.487 kb on + strand at 3016.578 kb on + strand, within Pf1N1B4_2678 at 3016.578 kb on + strand, within Pf1N1B4_2678 at 3016.603 kb on - strand, within Pf1N1B4_2678 at 3016.738 kb on + strand at 3016.764 kb on + strand at 3016.764 kb on + strand at 3016.764 kb on + strand at 3016.765 kb on - strand at 3016.765 kb on - strand at 3016.765 kb on - strand at 3016.765 kb on - strand at 3016.765 kb on - strand at 3016.765 kb on - strand at 3016.810 kb on + strand at 3016.810 kb on + strand at 3016.810 kb on + strand at 3016.810 kb on + strand at 3016.810 kb on + strand at 3016.811 kb on - strand at 3016.811 kb on - strand at 3016.811 kb on - strand at 3016.811 kb on - strand at 3016.832 kb on + strand at 3016.832 kb on + strand at 3016.832 kb on + strand at 3016.832 kb on + strand at 3016.832 kb on + strand at 3016.832 kb on + strand at 3016.833 kb on - strand at 3016.833 kb on - strand at 3016.833 kb on - strand at 3016.833 kb on - strand at 3016.833 kb on - strand at 3016.833 kb on - strand at 3016.833 kb on - strand at 3016.903 kb on + strand at 3016.903 kb on + strand at 3016.903 kb on + strand at 3016.903 kb on + strand at 3016.903 kb on + strand at 3016.903 kb on + strand at 3016.904 kb on - strand at 3016.904 kb on - strand at 3016.904 kb on - strand at 3016.907 kb on + strand at 3016.907 kb on + strand at 3016.907 kb on + strand at 3016.908 kb on - strand at 3016.908 kb on - strand at 3016.908 kb on - strand at 3016.908 kb on - strand at 3016.908 kb on - strand at 3016.908 kb on - strand at 3016.908 kb on - strand at 3016.908 kb on - strand at 3016.908 kb on - strand at 3016.911 kb on + strand at 3016.912 kb on - strand at 3016.912 kb on - strand at 3016.912 kb on - strand at 3016.912 kb on - strand at 3017.014 kb on + strand, within Pf1N1B4_2679 at 3017.014 kb on + strand, within Pf1N1B4_2679 at 3017.014 kb on + strand, within Pf1N1B4_2679 at 3017.015 kb on - strand at 3017.015 kb on - strand, within Pf1N1B4_2679 at 3017.015 kb on - strand, within Pf1N1B4_2679 at 3017.015 kb on - strand, within Pf1N1B4_2679 at 3017.170 kb on + strand, within Pf1N1B4_2679 at 3017.171 kb on - strand, within Pf1N1B4_2679 at 3017.171 kb on - strand at 3017.171 kb on - strand, within Pf1N1B4_2679 at 3017.439 kb on + strand, within Pf1N1B4_2679 at 3017.442 kb on + strand, within Pf1N1B4_2679 at 3017.443 kb on - strand, within Pf1N1B4_2679 at 3017.443 kb on - strand, within Pf1N1B4_2679 at 3017.450 kb on + strand, within Pf1N1B4_2679 at 3017.451 kb on - strand, within Pf1N1B4_2679 at 3017.470 kb on - strand, within Pf1N1B4_2679
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 3,015,489 + Pf1N1B4_2676 0.72 +2.1 3,015,489 + -1.0 3,016,487 + +0.0 3,016,487 + -0.5 3,016,487 + +0.1 3,016,487 + +1.3 3,016,578 + Pf1N1B4_2678 0.34 -0.2 3,016,578 + Pf1N1B4_2678 0.34 -0.2 3,016,603 - Pf1N1B4_2678 0.42 +2.0 3,016,738 + -0.2 3,016,764 + +0.5 3,016,764 + +2.1 3,016,764 + -0.4 3,016,765 - +0.3 3,016,765 - +1.3 3,016,765 - -0.4 3,016,765 - +0.8 3,016,765 - -0.2 3,016,765 - +1.7 3,016,810 + +1.3 3,016,810 + +0.0 3,016,810 + -0.1 3,016,810 + +1.3 3,016,810 + +0.9 3,016,811 - +2.1 3,016,811 - +0.9 3,016,811 - +1.9 3,016,811 - +1.3 3,016,832 + +0.9 3,016,832 + +1.1 3,016,832 + +0.3 3,016,832 + -1.0 3,016,832 + +0.8 3,016,832 + +1.9 3,016,833 - +1.3 3,016,833 - +1.0 3,016,833 - +0.2 3,016,833 - -0.2 3,016,833 - -1.0 3,016,833 - -0.3 3,016,833 - +2.7 3,016,903 + -0.7 3,016,903 + -0.7 3,016,903 + -0.2 3,016,903 + -0.7 3,016,903 + -1.2 3,016,903 + -0.2 3,016,904 - +0.8 3,016,904 - -0.8 3,016,904 - +0.3 3,016,907 + +1.9 3,016,907 + +0.2 3,016,907 + -0.0 3,016,908 - -0.1 3,016,908 - +3.3 3,016,908 - +1.1 3,016,908 - +0.3 3,016,908 - -1.7 3,016,908 - -1.4 3,016,908 - -0.4 3,016,908 - -0.8 3,016,908 - +0.3 3,016,911 + -1.0 3,016,912 - -0.7 3,016,912 - +1.0 3,016,912 - -0.7 3,016,912 - -1.2 3,017,014 + Pf1N1B4_2679 0.17 +0.2 3,017,014 + Pf1N1B4_2679 0.17 -0.7 3,017,014 + Pf1N1B4_2679 0.17 -0.2 3,017,015 - -1.2 3,017,015 - Pf1N1B4_2679 0.18 -1.2 3,017,015 - Pf1N1B4_2679 0.18 +0.6 3,017,015 - Pf1N1B4_2679 0.18 -0.2 3,017,170 + Pf1N1B4_2679 0.42 +0.0 3,017,171 - Pf1N1B4_2679 0.42 +1.3 3,017,171 - +0.3 3,017,171 - Pf1N1B4_2679 0.42 +0.1 3,017,439 + Pf1N1B4_2679 0.84 +0.1 3,017,442 + Pf1N1B4_2679 0.84 +0.3 3,017,443 - Pf1N1B4_2679 0.84 -0.7 3,017,443 - Pf1N1B4_2679 0.84 -0.2 3,017,450 + Pf1N1B4_2679 0.85 -0.5 3,017,451 - Pf1N1B4_2679 0.86 -1.0 3,017,470 - Pf1N1B4_2679 0.88 +1.3
Or see this region's nucleotide sequence