Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2673 and Pf1N1B4_2674 are separated by 103 nucleotides Pf1N1B4_2674 and Pf1N1B4_2675 overlap by 15 nucleotides
Pf1N1B4_2673 - Aldehyde dehydrogenase (EC 1.2.1.3), at 3,010,438 to 3,011,958
_2673
Pf1N1B4_2674 - hypothetical protein, at 3,012,062 to 3,012,424
_2674
Pf1N1B4_2675 - Nitrogen regulation protein NR(I), at 3,012,410 to 3,014,323
_2675
Position (kb)
3012
3013 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3011.274 kb on + strand, within Pf1N1B4_2673 at 3011.412 kb on + strand, within Pf1N1B4_2673 at 3011.514 kb on - strand, within Pf1N1B4_2673 at 3011.820 kb on + strand at 3011.820 kb on + strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.845 kb on - strand at 3011.845 kb on - strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.899 kb on - strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.904 kb on - strand at 3011.904 kb on - strand at 3011.904 kb on - strand at 3011.951 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.987 kb on - strand at 3011.987 kb on - strand at 3011.987 kb on - strand at 3011.987 kb on - strand at 3011.988 kb on + strand at 3011.988 kb on + strand at 3011.988 kb on + strand at 3011.988 kb on + strand at 3011.989 kb on - strand at 3012.016 kb on + strand at 3012.023 kb on - strand at 3012.058 kb on + strand at 3012.233 kb on + strand, within Pf1N1B4_2674 at 3012.234 kb on - strand, within Pf1N1B4_2674 at 3012.372 kb on - strand, within Pf1N1B4_2674 at 3012.401 kb on + strand at 3012.404 kb on + strand at 3012.404 kb on + strand at 3012.404 kb on + strand at 3012.405 kb on - strand at 3012.405 kb on - strand at 3012.405 kb on - strand at 3012.536 kb on + strand at 3012.537 kb on - strand at 3012.537 kb on - strand at 3012.537 kb on - strand at 3012.806 kb on - strand, within Pf1N1B4_2675 at 3012.883 kb on + strand, within Pf1N1B4_2675 at 3012.884 kb on - strand, within Pf1N1B4_2675 at 3013.040 kb on + strand, within Pf1N1B4_2675 at 3013.040 kb on + strand, within Pf1N1B4_2675 at 3013.040 kb on + strand, within Pf1N1B4_2675 at 3013.041 kb on - strand, within Pf1N1B4_2675 at 3013.041 kb on - strand at 3013.041 kb on - strand, within Pf1N1B4_2675 at 3013.052 kb on + strand, within Pf1N1B4_2675 at 3013.052 kb on + strand, within Pf1N1B4_2675 at 3013.053 kb on - strand, within Pf1N1B4_2675 at 3013.189 kb on + strand, within Pf1N1B4_2675 at 3013.348 kb on + strand, within Pf1N1B4_2675 at 3013.348 kb on + strand, within Pf1N1B4_2675 at 3013.348 kb on + strand, within Pf1N1B4_2675 at 3013.349 kb on - strand, within Pf1N1B4_2675
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 3,011,274 + Pf1N1B4_2673 0.55 +0.3 3,011,412 + Pf1N1B4_2673 0.64 -0.2 3,011,514 - Pf1N1B4_2673 0.71 -0.3 3,011,820 + +2.7 3,011,820 + +0.5 3,011,821 - -0.6 3,011,821 - +0.1 3,011,821 - -1.0 3,011,821 - +0.2 3,011,821 - -1.5 3,011,821 - -2.0 3,011,845 - +1.0 3,011,845 - -1.8 3,011,894 + +0.9 3,011,894 + +0.7 3,011,894 + -0.2 3,011,894 + +1.3 3,011,894 + -1.6 3,011,894 + +0.0 3,011,894 + +0.8 3,011,894 + +0.9 3,011,894 + +0.4 3,011,894 + -0.5 3,011,894 + -0.2 3,011,895 - +0.7 3,011,895 - +0.3 3,011,895 - -1.2 3,011,895 - +0.7 3,011,895 - -1.0 3,011,895 - +0.6 3,011,895 - +1.3 3,011,895 - -1.2 3,011,899 - -0.2 3,011,903 + -0.1 3,011,903 + -0.8 3,011,903 + +1.3 3,011,903 + +0.8 3,011,903 + -0.5 3,011,903 + -0.7 3,011,903 + -1.2 3,011,904 - -0.8 3,011,904 - +0.7 3,011,904 - -1.0 3,011,951 + +0.2 3,011,986 + +0.6 3,011,986 + -0.1 3,011,986 + -0.4 3,011,986 + +1.9 3,011,986 + +2.3 3,011,986 + +0.3 3,011,986 + -0.5 3,011,987 - -0.4 3,011,987 - +1.3 3,011,987 - +0.3 3,011,987 - -1.2 3,011,988 + -0.3 3,011,988 + +0.9 3,011,988 + +1.2 3,011,988 + -0.7 3,011,989 - -1.8 3,012,016 + -0.3 3,012,023 - -1.0 3,012,058 + +0.3 3,012,233 + Pf1N1B4_2674 0.47 -1.7 3,012,234 - Pf1N1B4_2674 0.47 +0.2 3,012,372 - Pf1N1B4_2674 0.85 +0.3 3,012,401 + +0.0 3,012,404 + +0.0 3,012,404 + -0.7 3,012,404 + +0.3 3,012,405 - -1.7 3,012,405 - -0.7 3,012,405 - -0.2 3,012,536 + +0.3 3,012,537 - -0.4 3,012,537 - -0.1 3,012,537 - -2.5 3,012,806 - Pf1N1B4_2675 0.21 +0.6 3,012,883 + Pf1N1B4_2675 0.25 -1.2 3,012,884 - Pf1N1B4_2675 0.25 -1.2 3,013,040 + Pf1N1B4_2675 0.33 +1.3 3,013,040 + Pf1N1B4_2675 0.33 +1.1 3,013,040 + Pf1N1B4_2675 0.33 -0.7 3,013,041 - Pf1N1B4_2675 0.33 +0.1 3,013,041 - +0.3 3,013,041 - Pf1N1B4_2675 0.33 +0.3 3,013,052 + Pf1N1B4_2675 0.34 -0.5 3,013,052 + Pf1N1B4_2675 0.34 +0.1 3,013,053 - Pf1N1B4_2675 0.34 +0.5 3,013,189 + Pf1N1B4_2675 0.41 -0.1 3,013,348 + Pf1N1B4_2675 0.49 -1.7 3,013,348 + Pf1N1B4_2675 0.49 -1.5 3,013,348 + Pf1N1B4_2675 0.49 -0.2 3,013,349 - Pf1N1B4_2675 0.49 +0.8
Or see this region's nucleotide sequence