Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2636 and Pf1N1B4_2637 are separated by 32 nucleotides Pf1N1B4_2637 and Pf1N1B4_2638 overlap by 8 nucleotides Pf1N1B4_2638 and Pf1N1B4_2639 are separated by 157 nucleotides
Pf1N1B4_2636 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 2,977,295 to 2,977,411
_2636
Pf1N1B4_2637 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 2,977,444 to 2,978,163
_2637
Pf1N1B4_2638 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 2,978,156 to 2,979,085
_2638
Pf1N1B4_2639 - Outer membrane protein W precursor, at 2,979,243 to 2,979,941
_2639
Position (kb)
2978
2979
2980 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2977.160 kb on - strand at 2977.210 kb on + strand at 2977.279 kb on + strand at 2977.279 kb on + strand at 2977.279 kb on + strand at 2977.279 kb on + strand at 2977.279 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.299 kb on + strand at 2977.299 kb on + strand at 2977.299 kb on + strand at 2977.459 kb on + strand at 2977.459 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.585 kb on + strand, within Pf1N1B4_2637 at 2977.618 kb on + strand, within Pf1N1B4_2637 at 2977.618 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2978.135 kb on + strand at 2978.142 kb on + strand at 2978.142 kb on + strand at 2978.149 kb on + strand at 2978.149 kb on + strand at 2979.090 kb on - strand at 2979.244 kb on + strand at 2979.245 kb on - strand at 2979.245 kb on - strand at 2979.271 kb on + strand at 2979.271 kb on + strand at 2979.271 kb on + strand at 2979.271 kb on + strand at 2979.271 kb on + strand at 2979.271 kb on + strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.364 kb on + strand, within Pf1N1B4_2639 at 2979.364 kb on + strand, within Pf1N1B4_2639 at 2979.365 kb on - strand, within Pf1N1B4_2639 at 2979.365 kb on - strand, within Pf1N1B4_2639 at 2979.365 kb on - strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.458 kb on + strand, within Pf1N1B4_2639 at 2979.458 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.515 kb on - strand, within Pf1N1B4_2639 at 2979.515 kb on - strand, within Pf1N1B4_2639 at 2979.515 kb on - strand, within Pf1N1B4_2639 at 2979.515 kb on - strand, within Pf1N1B4_2639 at 2979.515 kb on - strand, within Pf1N1B4_2639 at 2979.515 kb on - strand, within Pf1N1B4_2639 at 2979.521 kb on + strand, within Pf1N1B4_2639 at 2979.535 kb on + strand, within Pf1N1B4_2639 at 2979.535 kb on + strand, within Pf1N1B4_2639 at 2979.535 kb on + strand, within Pf1N1B4_2639 at 2979.535 kb on + strand, within Pf1N1B4_2639 at 2979.536 kb on - strand at 2979.536 kb on - strand, within Pf1N1B4_2639 at 2979.536 kb on - strand, within Pf1N1B4_2639 at 2979.536 kb on - strand, within Pf1N1B4_2639 at 2979.568 kb on + strand, within Pf1N1B4_2639 at 2979.571 kb on + strand, within Pf1N1B4_2639 at 2979.571 kb on + strand, within Pf1N1B4_2639 at 2979.571 kb on + strand, within Pf1N1B4_2639 at 2979.571 kb on + strand, within Pf1N1B4_2639 at 2979.571 kb on + strand, within Pf1N1B4_2639 at 2979.571 kb on + strand, within Pf1N1B4_2639 at 2979.572 kb on - strand, within Pf1N1B4_2639 at 2979.572 kb on - strand, within Pf1N1B4_2639 at 2979.572 kb on - strand, within Pf1N1B4_2639 at 2979.572 kb on - strand, within Pf1N1B4_2639 at 2979.715 kb on + strand, within Pf1N1B4_2639 at 2979.715 kb on + strand, within Pf1N1B4_2639 at 2979.716 kb on - strand, within Pf1N1B4_2639 at 2979.716 kb on - strand, within Pf1N1B4_2639 at 2979.984 kb on + strand at 2979.984 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 2,977,160 - +1.2 2,977,210 + -0.6 2,977,279 + +0.5 2,977,279 + +2.4 2,977,279 + -1.0 2,977,279 + -0.5 2,977,279 + +1.4 2,977,294 + +1.6 2,977,294 + +0.7 2,977,294 + -0.4 2,977,294 + -0.6 2,977,294 + +1.3 2,977,294 + -0.0 2,977,294 + +2.0 2,977,294 + +0.7 2,977,294 + +0.3 2,977,294 + +0.2 2,977,294 + -1.6 2,977,294 + +1.6 2,977,294 + -0.4 2,977,294 + +0.1 2,977,294 + -0.5 2,977,294 + +0.0 2,977,294 + +1.1 2,977,294 + +3.2 2,977,294 + +2.2 2,977,294 + +0.3 2,977,294 + +0.8 2,977,294 + +1.0 2,977,294 + +2.2 2,977,294 + +0.6 2,977,294 + +0.2 2,977,294 + +1.1 2,977,294 + -0.6 2,977,294 + +0.8 2,977,294 + +2.1 2,977,294 + +1.9 2,977,299 + +0.3 2,977,299 + -0.2 2,977,299 + +2.2 2,977,459 + +2.0 2,977,459 + +0.8 2,977,462 + +0.4 2,977,462 + +0.9 2,977,462 + -1.6 2,977,462 + +0.7 2,977,462 + +2.2 2,977,462 + +1.4 2,977,462 + +0.8 2,977,462 + +1.4 2,977,462 + +0.9 2,977,462 + +1.5 2,977,462 + +1.0 2,977,462 + +1.4 2,977,462 + +1.4 2,977,462 + +1.1 2,977,462 + +1.1 2,977,462 + +0.3 2,977,462 + +1.8 2,977,462 + +1.1 2,977,585 + Pf1N1B4_2637 0.20 +0.8 2,977,618 + Pf1N1B4_2637 0.24 +0.4 2,977,618 + Pf1N1B4_2637 0.24 +1.5 2,977,909 + Pf1N1B4_2637 0.65 +1.2 2,977,909 + Pf1N1B4_2637 0.65 +2.0 2,977,909 + Pf1N1B4_2637 0.65 +2.5 2,977,909 + Pf1N1B4_2637 0.65 +1.9 2,977,909 + Pf1N1B4_2637 0.65 +2.2 2,977,909 + Pf1N1B4_2637 0.65 +1.2 2,977,909 + Pf1N1B4_2637 0.65 +1.4 2,977,909 + Pf1N1B4_2637 0.65 +0.1 2,977,909 + Pf1N1B4_2637 0.65 +1.0 2,977,909 + Pf1N1B4_2637 0.65 +2.1 2,977,909 + Pf1N1B4_2637 0.65 +2.2 2,977,909 + +0.8 2,977,909 + Pf1N1B4_2637 0.65 +0.1 2,977,909 + Pf1N1B4_2637 0.65 +1.7 2,977,909 + Pf1N1B4_2637 0.65 +1.6 2,977,909 + Pf1N1B4_2637 0.65 +1.0 2,977,909 + Pf1N1B4_2637 0.65 +2.7 2,977,909 + Pf1N1B4_2637 0.65 +0.1 2,977,909 + Pf1N1B4_2637 0.65 +0.9 2,977,909 + Pf1N1B4_2637 0.65 +1.0 2,978,135 + +0.4 2,978,142 + +0.8 2,978,142 + +0.9 2,978,149 + -0.1 2,978,149 + +1.0 2,979,090 - -0.0 2,979,244 + -0.4 2,979,245 - -1.0 2,979,245 - +0.4 2,979,271 + -1.1 2,979,271 + -0.6 2,979,271 + +0.6 2,979,271 + -0.4 2,979,271 + -0.2 2,979,271 + +0.6 2,979,272 - -1.4 2,979,272 - -0.2 2,979,272 - +0.4 2,979,272 - -1.6 2,979,272 - -0.0 2,979,272 - -0.9 2,979,272 - +0.1 2,979,272 - -0.7 2,979,272 - -0.2 2,979,272 - +0.7 2,979,364 + Pf1N1B4_2639 0.17 -0.5 2,979,364 + Pf1N1B4_2639 0.17 -1.8 2,979,365 - Pf1N1B4_2639 0.17 -1.0 2,979,365 - Pf1N1B4_2639 0.17 +0.2 2,979,365 - Pf1N1B4_2639 0.17 +0.3 2,979,387 + Pf1N1B4_2639 0.21 -1.2 2,979,387 + Pf1N1B4_2639 0.21 +1.4 2,979,387 + Pf1N1B4_2639 0.21 +2.4 2,979,387 + Pf1N1B4_2639 0.21 +0.4 2,979,387 + Pf1N1B4_2639 0.21 -0.0 2,979,387 + Pf1N1B4_2639 0.21 +1.0 2,979,387 + Pf1N1B4_2639 0.21 -0.2 2,979,387 + Pf1N1B4_2639 0.21 -0.2 2,979,387 + Pf1N1B4_2639 0.21 -1.1 2,979,388 - Pf1N1B4_2639 0.21 -1.4 2,979,388 - Pf1N1B4_2639 0.21 +1.1 2,979,388 - Pf1N1B4_2639 0.21 -0.6 2,979,388 - Pf1N1B4_2639 0.21 +1.4 2,979,388 - Pf1N1B4_2639 0.21 +0.7 2,979,388 - Pf1N1B4_2639 0.21 -0.2 2,979,388 - Pf1N1B4_2639 0.21 -0.4 2,979,406 + Pf1N1B4_2639 0.23 -0.6 2,979,406 + Pf1N1B4_2639 0.23 -0.1 2,979,406 + Pf1N1B4_2639 0.23 -2.4 2,979,406 + Pf1N1B4_2639 0.23 -1.0 2,979,406 + Pf1N1B4_2639 0.23 +1.4 2,979,406 + Pf1N1B4_2639 0.23 -2.2 2,979,406 + Pf1N1B4_2639 0.23 +1.0 2,979,406 + Pf1N1B4_2639 0.23 +0.6 2,979,407 - Pf1N1B4_2639 0.23 +0.7 2,979,407 - Pf1N1B4_2639 0.23 +0.9 2,979,407 - Pf1N1B4_2639 0.23 -0.2 2,979,407 - Pf1N1B4_2639 0.23 -1.0 2,979,407 - Pf1N1B4_2639 0.23 -0.2 2,979,407 - Pf1N1B4_2639 0.23 +1.6 2,979,407 - Pf1N1B4_2639 0.23 +0.8 2,979,458 + Pf1N1B4_2639 0.31 -0.2 2,979,458 + Pf1N1B4_2639 0.31 -0.6 2,979,514 + Pf1N1B4_2639 0.39 -1.8 2,979,514 + Pf1N1B4_2639 0.39 +0.8 2,979,514 + Pf1N1B4_2639 0.39 -0.7 2,979,514 + Pf1N1B4_2639 0.39 -0.6 2,979,514 + Pf1N1B4_2639 0.39 +0.0 2,979,514 + Pf1N1B4_2639 0.39 -0.6 2,979,514 + Pf1N1B4_2639 0.39 -0.4 2,979,514 + Pf1N1B4_2639 0.39 -0.2 2,979,514 + Pf1N1B4_2639 0.39 -0.5 2,979,514 + Pf1N1B4_2639 0.39 +0.0 2,979,514 + Pf1N1B4_2639 0.39 -1.2 2,979,514 + Pf1N1B4_2639 0.39 -0.2 2,979,514 + Pf1N1B4_2639 0.39 +0.7 2,979,515 - Pf1N1B4_2639 0.39 +0.1 2,979,515 - Pf1N1B4_2639 0.39 +0.1 2,979,515 - Pf1N1B4_2639 0.39 -0.4 2,979,515 - Pf1N1B4_2639 0.39 -1.1 2,979,515 - Pf1N1B4_2639 0.39 -2.6 2,979,515 - Pf1N1B4_2639 0.39 +0.0 2,979,521 + Pf1N1B4_2639 0.40 -0.4 2,979,535 + Pf1N1B4_2639 0.42 -0.5 2,979,535 + Pf1N1B4_2639 0.42 -0.2 2,979,535 + Pf1N1B4_2639 0.42 +1.4 2,979,535 + Pf1N1B4_2639 0.42 +0.9 2,979,536 - -0.2 2,979,536 - Pf1N1B4_2639 0.42 -0.6 2,979,536 - Pf1N1B4_2639 0.42 +1.4 2,979,536 - Pf1N1B4_2639 0.42 -0.6 2,979,568 + Pf1N1B4_2639 0.46 +0.2 2,979,571 + Pf1N1B4_2639 0.47 -1.0 2,979,571 + Pf1N1B4_2639 0.47 +0.1 2,979,571 + Pf1N1B4_2639 0.47 -0.7 2,979,571 + Pf1N1B4_2639 0.47 -1.6 2,979,571 + Pf1N1B4_2639 0.47 +0.4 2,979,571 + Pf1N1B4_2639 0.47 -1.4 2,979,572 - Pf1N1B4_2639 0.47 +0.4 2,979,572 - Pf1N1B4_2639 0.47 +1.0 2,979,572 - Pf1N1B4_2639 0.47 -1.4 2,979,572 - Pf1N1B4_2639 0.47 -1.0 2,979,715 + Pf1N1B4_2639 0.68 -1.1 2,979,715 + Pf1N1B4_2639 0.68 -0.4 2,979,716 - Pf1N1B4_2639 0.68 +0.7 2,979,716 - Pf1N1B4_2639 0.68 +1.4 2,979,984 + +0.2 2,979,984 + -0.6
Or see this region's nucleotide sequence