Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2474

Experiment: Choline Lysine 6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2473 and Pf1N1B4_2474 overlap by 4 nucleotidesPf1N1B4_2474 and Pf1N1B4_2475 are separated by 291 nucleotidesPf1N1B4_2475 and Pf1N1B4_2476 overlap by 4 nucleotides Pf1N1B4_2473 - Transmembrane sensor, at 2,805,111 to 2,806,085 _2473 Pf1N1B4_2474 - RNA polymerase sigma-70 factor, ECF subfamily, at 2,806,082 to 2,806,558 _2474 Pf1N1B4_2475 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 2,806,850 to 2,807,557 _2475 Pf1N1B4_2476 - Predicted signal-transduction protein containing cAMP-binding and CBS domains, at 2,807,554 to 2,809,494 _2476 Position (kb) 2806 2807Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2805.758 kb on - strand, within Pf1N1B4_2473at 2806.098 kb on + strandat 2806.098 kb on + strandat 2806.098 kb on + strandat 2806.099 kb on - strandat 2806.099 kb on - strandat 2806.099 kb on - strandat 2806.099 kb on - strandat 2806.099 kb on - strandat 2806.110 kb on + strandat 2806.110 kb on + strandat 2806.110 kb on + strandat 2806.110 kb on + strandat 2806.110 kb on + strandat 2806.110 kb on + strandat 2806.110 kb on + strandat 2806.111 kb on - strandat 2806.111 kb on - strandat 2806.111 kb on - strandat 2806.111 kb on - strandat 2806.111 kb on - strandat 2806.137 kb on + strand, within Pf1N1B4_2474at 2806.138 kb on - strand, within Pf1N1B4_2474at 2806.212 kb on + strand, within Pf1N1B4_2474at 2806.212 kb on + strand, within Pf1N1B4_2474at 2806.213 kb on - strand, within Pf1N1B4_2474at 2806.213 kb on - strand, within Pf1N1B4_2474at 2806.213 kb on - strand, within Pf1N1B4_2474at 2806.213 kb on - strand, within Pf1N1B4_2474at 2806.347 kb on + strand, within Pf1N1B4_2474at 2806.347 kb on + strand, within Pf1N1B4_2474at 2806.348 kb on - strand, within Pf1N1B4_2474at 2806.348 kb on - strand, within Pf1N1B4_2474at 2806.348 kb on - strand, within Pf1N1B4_2474at 2806.348 kb on - strand, within Pf1N1B4_2474at 2806.377 kb on + strand, within Pf1N1B4_2474at 2806.378 kb on - strand, within Pf1N1B4_2474at 2806.378 kb on - strand, within Pf1N1B4_2474at 2806.532 kb on + strandat 2806.532 kb on + strandat 2806.533 kb on - strandat 2806.533 kb on - strandat 2806.554 kb on + strandat 2806.669 kb on + strandat 2806.669 kb on + strandat 2806.670 kb on - strandat 2806.851 kb on + strandat 2806.851 kb on + strandat 2806.851 kb on + strandat 2806.915 kb on + strandat 2806.916 kb on - strandat 2807.121 kb on + strand, within Pf1N1B4_2475at 2807.122 kb on - strand, within Pf1N1B4_2475at 2807.395 kb on + strand, within Pf1N1B4_2475at 2807.429 kb on + strand, within Pf1N1B4_2475at 2807.557 kb on + strandat 2807.557 kb on + strandat 2807.557 kb on + strandat 2807.558 kb on - strandat 2807.558 kb on - strandat 2807.558 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 6 mM
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2,805,758 - Pf1N1B4_2473 0.66 -0.4
2,806,098 + +0.3
2,806,098 + -1.6
2,806,098 + -0.2
2,806,099 - +2.1
2,806,099 - -0.9
2,806,099 - -0.5
2,806,099 - +0.7
2,806,099 - +0.5
2,806,110 + +0.4
2,806,110 + +0.4
2,806,110 + -0.6
2,806,110 + +1.8
2,806,110 + +1.4
2,806,110 + +0.4
2,806,110 + -0.4
2,806,111 - +0.9
2,806,111 - -0.3
2,806,111 - -2.7
2,806,111 - -0.8
2,806,111 - +0.1
2,806,137 + Pf1N1B4_2474 0.12 -1.2
2,806,138 - Pf1N1B4_2474 0.12 +2.4
2,806,212 + Pf1N1B4_2474 0.27 -1.9
2,806,212 + Pf1N1B4_2474 0.27 +1.1
2,806,213 - Pf1N1B4_2474 0.27 -0.2
2,806,213 - Pf1N1B4_2474 0.27 -0.0
2,806,213 - Pf1N1B4_2474 0.27 -2.0
2,806,213 - Pf1N1B4_2474 0.27 +0.1
2,806,347 + Pf1N1B4_2474 0.56 -2.9
2,806,347 + Pf1N1B4_2474 0.56 -0.6
2,806,348 - Pf1N1B4_2474 0.56 -0.7
2,806,348 - Pf1N1B4_2474 0.56 -0.5
2,806,348 - Pf1N1B4_2474 0.56 +3.0
2,806,348 - Pf1N1B4_2474 0.56 -1.2
2,806,377 + Pf1N1B4_2474 0.62 -1.1
2,806,378 - Pf1N1B4_2474 0.62 -1.4
2,806,378 - Pf1N1B4_2474 0.62 -0.6
2,806,532 + -0.5
2,806,532 + +0.1
2,806,533 - -0.6
2,806,533 - +0.1
2,806,554 + +1.0
2,806,669 + -0.5
2,806,669 + -0.7
2,806,670 - +1.4
2,806,851 + -0.4
2,806,851 + +1.1
2,806,851 + -0.9
2,806,915 + -0.9
2,806,916 - +0.4
2,807,121 + Pf1N1B4_2475 0.38 +0.9
2,807,122 - Pf1N1B4_2475 0.38 +0.4
2,807,395 + Pf1N1B4_2475 0.77 +0.6
2,807,429 + Pf1N1B4_2475 0.82 -0.6
2,807,557 + -0.6
2,807,557 + -1.6
2,807,557 + -0.2
2,807,558 - +0.7
2,807,558 - -0.2
2,807,558 - +0.1

Or see this region's nucleotide sequence