Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2457 and Pf1N1B4_2458 are separated by 129 nucleotides Pf1N1B4_2458 and Pf1N1B4_2459 overlap by 17 nucleotides Pf1N1B4_2459 and Pf1N1B4_2460 are separated by 6 nucleotides Pf1N1B4_2460 and Pf1N1B4_2461 are separated by 205 nucleotides
Pf1N1B4_2457 - Sarcosine oxidase alpha subunit (EC 1.5.3.1), at 2,786,534 to 2,789,551
_2457
Pf1N1B4_2458 - Sarcosine oxidase gamma subunit (EC 1.5.3.1), at 2,789,681 to 2,790,313
_2458
Pf1N1B4_2459 - hypothetical protein, at 2,790,297 to 2,790,440
_2459
Pf1N1B4_2460 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 2,790,447 to 2,791,304
_2460
Pf1N1B4_2461 - Threonine dehydrogenase and related Zn-dependent dehydrogenases, at 2,791,510 to 2,792,709
_2461
Position (kb)
2790
2791
2792 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2789.549 kb on + strand at 2789.549 kb on + strand at 2789.550 kb on - strand at 2789.550 kb on - strand at 2789.680 kb on + strand at 2789.681 kb on - strand at 2790.096 kb on + strand, within Pf1N1B4_2458 at 2790.097 kb on - strand, within Pf1N1B4_2458 at 2790.125 kb on + strand, within Pf1N1B4_2458 at 2790.125 kb on + strand, within Pf1N1B4_2458 at 2790.125 kb on + strand, within Pf1N1B4_2458 at 2790.126 kb on - strand, within Pf1N1B4_2458 at 2790.126 kb on - strand, within Pf1N1B4_2458 at 2790.126 kb on - strand, within Pf1N1B4_2458 at 2790.142 kb on + strand, within Pf1N1B4_2458 at 2790.298 kb on + strand at 2790.312 kb on - strand at 2790.443 kb on + strand at 2790.444 kb on - strand at 2790.444 kb on - strand at 2790.446 kb on + strand at 2790.446 kb on + strand at 2790.446 kb on + strand at 2790.546 kb on + strand, within Pf1N1B4_2460 at 2790.547 kb on - strand, within Pf1N1B4_2460 at 2790.547 kb on - strand, within Pf1N1B4_2460 at 2790.722 kb on - strand, within Pf1N1B4_2460 at 2790.868 kb on - strand, within Pf1N1B4_2460 at 2790.966 kb on + strand, within Pf1N1B4_2460 at 2790.966 kb on + strand, within Pf1N1B4_2460 at 2790.966 kb on + strand, within Pf1N1B4_2460 at 2790.966 kb on + strand, within Pf1N1B4_2460 at 2790.967 kb on - strand, within Pf1N1B4_2460 at 2791.077 kb on + strand, within Pf1N1B4_2460 at 2791.078 kb on - strand, within Pf1N1B4_2460 at 2791.116 kb on + strand, within Pf1N1B4_2460 at 2791.116 kb on + strand, within Pf1N1B4_2460 at 2791.116 kb on + strand, within Pf1N1B4_2460 at 2791.116 kb on + strand, within Pf1N1B4_2460 at 2791.117 kb on - strand, within Pf1N1B4_2460 at 2791.117 kb on - strand, within Pf1N1B4_2460 at 2791.117 kb on - strand, within Pf1N1B4_2460 at 2791.117 kb on - strand, within Pf1N1B4_2460 at 2791.178 kb on + strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.302 kb on + strand at 2791.303 kb on - strand at 2791.363 kb on + strand at 2791.364 kb on - strand at 2791.364 kb on - strand at 2791.364 kb on - strand at 2791.482 kb on + strand at 2791.482 kb on + strand at 2791.483 kb on - strand at 2791.483 kb on - strand at 2791.483 kb on - strand at 2791.486 kb on + strand at 2791.486 kb on + strand at 2791.486 kb on + strand at 2791.486 kb on + strand at 2791.487 kb on - strand at 2791.487 kb on - strand at 2791.487 kb on - strand at 2791.487 kb on - strand at 2791.559 kb on + strand at 2791.680 kb on + strand, within Pf1N1B4_2461 at 2791.681 kb on - strand, within Pf1N1B4_2461 at 2791.833 kb on + strand, within Pf1N1B4_2461 at 2791.833 kb on + strand, within Pf1N1B4_2461 at 2791.833 kb on + strand, within Pf1N1B4_2461 at 2791.833 kb on + strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.879 kb on + strand, within Pf1N1B4_2461 at 2791.880 kb on - strand, within Pf1N1B4_2461 at 2791.880 kb on - strand, within Pf1N1B4_2461 at 2791.880 kb on - strand at 2791.880 kb on - strand, within Pf1N1B4_2461 at 2791.925 kb on - strand, within Pf1N1B4_2461 at 2791.939 kb on + strand, within Pf1N1B4_2461 at 2791.939 kb on + strand, within Pf1N1B4_2461 at 2792.080 kb on + strand, within Pf1N1B4_2461 at 2792.080 kb on + strand, within Pf1N1B4_2461 at 2792.081 kb on - strand at 2792.298 kb on - strand, within Pf1N1B4_2461
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 2,789,549 + +1.7 2,789,549 + +2.3 2,789,550 - +1.4 2,789,550 - +2.0 2,789,680 + -0.6 2,789,681 - -0.4 2,790,096 + Pf1N1B4_2458 0.66 -1.4 2,790,097 - Pf1N1B4_2458 0.66 +0.6 2,790,125 + Pf1N1B4_2458 0.70 -0.5 2,790,125 + Pf1N1B4_2458 0.70 -1.8 2,790,125 + Pf1N1B4_2458 0.70 -1.8 2,790,126 - Pf1N1B4_2458 0.70 +1.2 2,790,126 - Pf1N1B4_2458 0.70 -1.4 2,790,126 - Pf1N1B4_2458 0.70 -0.2 2,790,142 + Pf1N1B4_2458 0.73 -1.8 2,790,298 + -0.1 2,790,312 - +0.5 2,790,443 + +1.7 2,790,444 - +0.1 2,790,444 - -0.8 2,790,446 + +1.4 2,790,446 + -0.3 2,790,446 + -0.1 2,790,546 + Pf1N1B4_2460 0.12 -0.9 2,790,547 - Pf1N1B4_2460 0.12 +1.4 2,790,547 - Pf1N1B4_2460 0.12 +0.1 2,790,722 - Pf1N1B4_2460 0.32 +0.4 2,790,868 - Pf1N1B4_2460 0.49 -2.1 2,790,966 + Pf1N1B4_2460 0.60 +0.6 2,790,966 + Pf1N1B4_2460 0.60 -1.4 2,790,966 + Pf1N1B4_2460 0.60 -0.6 2,790,966 + Pf1N1B4_2460 0.60 +0.6 2,790,967 - Pf1N1B4_2460 0.61 -2.8 2,791,077 + Pf1N1B4_2460 0.73 -0.9 2,791,078 - Pf1N1B4_2460 0.74 -0.1 2,791,116 + Pf1N1B4_2460 0.78 +0.1 2,791,116 + Pf1N1B4_2460 0.78 +0.2 2,791,116 + Pf1N1B4_2460 0.78 -0.9 2,791,116 + Pf1N1B4_2460 0.78 -0.8 2,791,117 - Pf1N1B4_2460 0.78 +0.4 2,791,117 - Pf1N1B4_2460 0.78 +0.1 2,791,117 - Pf1N1B4_2460 0.78 +0.4 2,791,117 - Pf1N1B4_2460 0.78 +1.0 2,791,178 + Pf1N1B4_2460 0.85 +1.1 2,791,179 - Pf1N1B4_2460 0.85 -1.2 2,791,179 - Pf1N1B4_2460 0.85 -0.6 2,791,179 - Pf1N1B4_2460 0.85 -1.1 2,791,179 - Pf1N1B4_2460 0.85 +2.0 2,791,179 - Pf1N1B4_2460 0.85 -1.8 2,791,179 - Pf1N1B4_2460 0.85 +0.6 2,791,302 + -0.1 2,791,303 - -2.1 2,791,363 + -0.6 2,791,364 - +0.2 2,791,364 - -0.2 2,791,364 - -0.0 2,791,482 + -1.4 2,791,482 + +0.0 2,791,483 - +0.4 2,791,483 - +1.6 2,791,483 - -0.9 2,791,486 + +0.4 2,791,486 + -0.7 2,791,486 + +1.2 2,791,486 + +2.1 2,791,487 - -0.4 2,791,487 - +1.4 2,791,487 - -0.9 2,791,487 - +0.8 2,791,559 + -0.5 2,791,680 + Pf1N1B4_2461 0.14 +0.2 2,791,681 - Pf1N1B4_2461 0.14 -0.5 2,791,833 + Pf1N1B4_2461 0.27 -1.5 2,791,833 + Pf1N1B4_2461 0.27 -1.3 2,791,833 + Pf1N1B4_2461 0.27 +1.2 2,791,833 + Pf1N1B4_2461 0.27 -0.2 2,791,834 - Pf1N1B4_2461 0.27 -0.4 2,791,834 - Pf1N1B4_2461 0.27 +0.7 2,791,834 - Pf1N1B4_2461 0.27 +0.4 2,791,834 - Pf1N1B4_2461 0.27 -1.8 2,791,834 - Pf1N1B4_2461 0.27 -0.7 2,791,834 - Pf1N1B4_2461 0.27 -0.3 2,791,834 - Pf1N1B4_2461 0.27 -1.4 2,791,879 + Pf1N1B4_2461 0.31 +1.4 2,791,880 - Pf1N1B4_2461 0.31 +1.4 2,791,880 - Pf1N1B4_2461 0.31 -0.6 2,791,880 - +0.4 2,791,880 - Pf1N1B4_2461 0.31 -1.2 2,791,925 - Pf1N1B4_2461 0.35 -0.4 2,791,939 + Pf1N1B4_2461 0.36 -1.4 2,791,939 + Pf1N1B4_2461 0.36 +1.1 2,792,080 + Pf1N1B4_2461 0.47 -0.0 2,792,080 + Pf1N1B4_2461 0.47 -0.9 2,792,081 - +1.4 2,792,298 - Pf1N1B4_2461 0.66 +0.3
Or see this region's nucleotide sequence