Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2414 and Pf1N1B4_2415 overlap by 1 nucleotides Pf1N1B4_2415 and Pf1N1B4_2416 are separated by 281 nucleotides
Pf1N1B4_2414 - TldE/PmbA family protein, Beta/Gamma-proteobacterial subgroup, at 2,735,474 to 2,736,823
_2414
Pf1N1B4_2415 - TldD family protein, Beta/Gamma-proteobacterial subgroup, at 2,736,823 to 2,738,265
_2415
Pf1N1B4_2416 - Choline dehydrogenase (EC 1.1.99.1), at 2,738,547 to 2,740,250
_2416
Position (kb)
2736
2737
2738
2739 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2735.865 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2736.000 kb on - strand, within Pf1N1B4_2414 at 2736.000 kb on - strand, within Pf1N1B4_2414 at 2736.000 kb on - strand, within Pf1N1B4_2414 at 2736.000 kb on - strand, within Pf1N1B4_2414 at 2736.000 kb on - strand, within Pf1N1B4_2414 at 2736.249 kb on + strand, within Pf1N1B4_2414 at 2736.250 kb on - strand, within Pf1N1B4_2414 at 2736.250 kb on - strand, within Pf1N1B4_2414 at 2736.250 kb on - strand, within Pf1N1B4_2414 at 2736.250 kb on - strand, within Pf1N1B4_2414 at 2736.576 kb on + strand, within Pf1N1B4_2414 at 2736.596 kb on - strand, within Pf1N1B4_2414 at 2736.717 kb on + strand at 2736.718 kb on - strand at 2736.797 kb on - strand at 2736.818 kb on - strand at 2736.931 kb on + strand at 2737.415 kb on + strand, within Pf1N1B4_2415 at 2737.464 kb on - strand, within Pf1N1B4_2415 at 2737.464 kb on - strand, within Pf1N1B4_2415 at 2737.725 kb on - strand, within Pf1N1B4_2415 at 2737.725 kb on - strand, within Pf1N1B4_2415 at 2737.796 kb on + strand, within Pf1N1B4_2415 at 2737.892 kb on + strand, within Pf1N1B4_2415 at 2737.892 kb on + strand, within Pf1N1B4_2415 at 2737.892 kb on + strand, within Pf1N1B4_2415 at 2737.892 kb on + strand, within Pf1N1B4_2415 at 2737.940 kb on + strand, within Pf1N1B4_2415 at 2737.940 kb on + strand, within Pf1N1B4_2415 at 2737.940 kb on + strand, within Pf1N1B4_2415 at 2738.075 kb on + strand, within Pf1N1B4_2415 at 2738.189 kb on + strand at 2738.297 kb on + strand at 2738.297 kb on + strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.335 kb on + strand at 2738.340 kb on + strand at 2738.340 kb on + strand at 2738.341 kb on - strand at 2738.358 kb on - strand at 2738.378 kb on + strand at 2738.540 kb on - strand at 2738.540 kb on - strand at 2738.540 kb on - strand at 2738.548 kb on + strand at 2738.611 kb on + strand at 2738.612 kb on - strand at 2738.612 kb on - strand at 2738.612 kb on - strand at 2738.612 kb on - strand at 2738.612 kb on - strand at 2738.614 kb on + strand at 2738.614 kb on + strand at 2738.615 kb on - strand at 2738.615 kb on - strand at 2739.004 kb on + strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.133 kb on + strand, within Pf1N1B4_2416 at 2739.133 kb on + strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.138 kb on - strand, within Pf1N1B4_2416 at 2739.138 kb on - strand, within Pf1N1B4_2416
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 2,735,865 + Pf1N1B4_2414 0.29 +0.1 2,735,999 + Pf1N1B4_2414 0.39 -0.6 2,735,999 + Pf1N1B4_2414 0.39 +1.2 2,735,999 + Pf1N1B4_2414 0.39 -1.2 2,735,999 + Pf1N1B4_2414 0.39 +0.8 2,735,999 + Pf1N1B4_2414 0.39 +0.4 2,735,999 + Pf1N1B4_2414 0.39 -0.5 2,735,999 + Pf1N1B4_2414 0.39 -1.6 2,736,000 - Pf1N1B4_2414 0.39 -1.8 2,736,000 - Pf1N1B4_2414 0.39 -1.4 2,736,000 - Pf1N1B4_2414 0.39 -0.6 2,736,000 - Pf1N1B4_2414 0.39 -0.4 2,736,000 - Pf1N1B4_2414 0.39 +0.9 2,736,249 + Pf1N1B4_2414 0.57 +0.1 2,736,250 - Pf1N1B4_2414 0.57 -1.6 2,736,250 - Pf1N1B4_2414 0.57 +0.8 2,736,250 - Pf1N1B4_2414 0.57 -0.2 2,736,250 - Pf1N1B4_2414 0.57 +0.7 2,736,576 + Pf1N1B4_2414 0.82 -0.8 2,736,596 - Pf1N1B4_2414 0.83 -0.2 2,736,717 + -0.2 2,736,718 - +0.8 2,736,797 - +1.0 2,736,818 - -0.3 2,736,931 + -0.3 2,737,415 + Pf1N1B4_2415 0.41 +0.5 2,737,464 - Pf1N1B4_2415 0.44 -1.6 2,737,464 - Pf1N1B4_2415 0.44 -0.6 2,737,725 - Pf1N1B4_2415 0.63 -1.6 2,737,725 - Pf1N1B4_2415 0.63 -1.2 2,737,796 + Pf1N1B4_2415 0.67 -1.4 2,737,892 + Pf1N1B4_2415 0.74 -2.1 2,737,892 + Pf1N1B4_2415 0.74 +0.3 2,737,892 + Pf1N1B4_2415 0.74 +0.5 2,737,892 + Pf1N1B4_2415 0.74 +0.7 2,737,940 + Pf1N1B4_2415 0.77 -1.8 2,737,940 + Pf1N1B4_2415 0.77 -2.7 2,737,940 + Pf1N1B4_2415 0.77 -0.8 2,738,075 + Pf1N1B4_2415 0.87 +0.2 2,738,189 + +1.1 2,738,297 + -0.2 2,738,297 + +1.4 2,738,298 - +0.1 2,738,298 - -0.6 2,738,298 - -1.2 2,738,298 - -0.0 2,738,298 - -0.1 2,738,298 - +0.1 2,738,335 + +0.4 2,738,340 + -0.6 2,738,340 + +1.5 2,738,341 - -1.1 2,738,358 - +0.4 2,738,378 + -0.1 2,738,540 - +1.4 2,738,540 - -0.4 2,738,540 - -1.6 2,738,548 + +1.2 2,738,611 + +0.4 2,738,612 - -0.9 2,738,612 - +1.1 2,738,612 - +1.8 2,738,612 - +1.9 2,738,612 - +0.2 2,738,614 + +1.7 2,738,614 + -0.2 2,738,615 - -0.3 2,738,615 - -0.6 2,739,004 + Pf1N1B4_2416 0.27 +1.7 2,739,005 - Pf1N1B4_2416 0.27 +0.9 2,739,005 - Pf1N1B4_2416 0.27 -0.5 2,739,005 - Pf1N1B4_2416 0.27 +0.4 2,739,005 - Pf1N1B4_2416 0.27 +0.5 2,739,005 - Pf1N1B4_2416 0.27 +0.4 2,739,133 + Pf1N1B4_2416 0.34 -0.6 2,739,133 + Pf1N1B4_2416 0.34 +1.7 2,739,134 - Pf1N1B4_2416 0.34 -0.2 2,739,134 - Pf1N1B4_2416 0.34 -1.4 2,739,134 - Pf1N1B4_2416 0.34 +0.7 2,739,134 - Pf1N1B4_2416 0.34 -0.3 2,739,134 - Pf1N1B4_2416 0.34 -0.2 2,739,134 - Pf1N1B4_2416 0.34 +1.0 2,739,138 - Pf1N1B4_2416 0.35 +0.8 2,739,138 - Pf1N1B4_2416 0.35 +2.0
Or see this region's nucleotide sequence