Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_237 and Pf1N1B4_238 are separated by 76 nucleotides Pf1N1B4_238 and Pf1N1B4_239 are separated by 89 nucleotides Pf1N1B4_239 and Pf1N1B4_240 are separated by 73 nucleotides Pf1N1B4_240 and Pf1N1B4_241 are separated by 163 nucleotides
Pf1N1B4_237 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 297,049 to 297,651
_237
Pf1N1B4_238 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 297,728 to 297,949
_238
Pf1N1B4_239 - Similar to ribosomal large subunit pseudouridine synthase A, at 298,039 to 298,929
_239
Pf1N1B4_240 - Transcriptional regulators, at 299,003 to 299,323
_240
Pf1N1B4_241 - Thiol-disulfide isomerase and thioredoxins, at 299,487 to 299,744
_241
Position (kb) 298
299 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.046 kb on - strand at 297.046 kb on - strand at 297.060 kb on + strand at 297.060 kb on + strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.238 kb on + strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.342 kb on - strand, within Pf1N1B4_237 at 297.543 kb on + strand, within Pf1N1B4_237 at 297.543 kb on + strand, within Pf1N1B4_237 at 297.609 kb on + strand at 297.623 kb on + strand at 297.677 kb on + strand at 297.677 kb on + strand at 297.678 kb on - strand at 297.678 kb on - strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.680 kb on - strand at 297.800 kb on - strand at 297.947 kb on + strand at 297.970 kb on + strand at 297.970 kb on + strand at 297.988 kb on + strand at 298.011 kb on - strand at 298.011 kb on - strand at 298.140 kb on - strand, within Pf1N1B4_239 at 298.140 kb on - strand, within Pf1N1B4_239 at 298.140 kb on - strand, within Pf1N1B4_239 at 298.151 kb on - strand, within Pf1N1B4_239 at 298.251 kb on - strand, within Pf1N1B4_239 at 298.360 kb on - strand, within Pf1N1B4_239 at 298.372 kb on - strand, within Pf1N1B4_239 at 298.713 kb on - strand, within Pf1N1B4_239 at 298.713 kb on - strand, within Pf1N1B4_239 at 298.713 kb on - strand, within Pf1N1B4_239 at 298.736 kb on + strand, within Pf1N1B4_239 at 298.737 kb on - strand at 298.880 kb on + strand at 298.880 kb on + strand at 298.881 kb on - strand at 298.881 kb on - strand at 298.953 kb on - strand at 298.990 kb on + strand at 298.990 kb on + strand at 298.991 kb on - strand at 298.991 kb on - strand at 299.103 kb on + strand, within Pf1N1B4_240 at 299.103 kb on + strand, within Pf1N1B4_240 at 299.103 kb on + strand, within Pf1N1B4_240 at 299.189 kb on - strand, within Pf1N1B4_240 at 299.357 kb on + strand at 299.411 kb on - strand at 299.576 kb on + strand, within Pf1N1B4_241 at 299.587 kb on + strand, within Pf1N1B4_241 at 299.750 kb on + strand at 299.750 kb on + strand at 299.750 kb on + strand at 299.751 kb on - strand at 299.751 kb on - strand at 299.763 kb on + strand at 299.764 kb on - strand at 299.798 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.901 kb on - strand at 299.901 kb on - strand at 299.901 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 297,045 + -1.0 297,045 + +0.6 297,045 + +0.8 297,045 + +1.1 297,045 + -2.0 297,046 - +0.8 297,046 - +0.8 297,060 + +0.2 297,060 + -2.4 297,061 - -1.2 297,061 - -0.5 297,061 - -0.4 297,238 + Pf1N1B4_237 0.31 -0.4 297,239 - Pf1N1B4_237 0.32 -0.8 297,239 - Pf1N1B4_237 0.32 -1.5 297,239 - Pf1N1B4_237 0.32 -2.1 297,341 + Pf1N1B4_237 0.48 -1.0 297,341 + Pf1N1B4_237 0.48 -1.0 297,341 + Pf1N1B4_237 0.48 +1.2 297,342 - Pf1N1B4_237 0.49 -1.2 297,543 + Pf1N1B4_237 0.82 +1.2 297,543 + Pf1N1B4_237 0.82 +0.5 297,609 + -0.7 297,623 + +0.8 297,677 + +1.4 297,677 + +0.2 297,678 - +0.8 297,678 - -1.0 297,679 + -0.5 297,679 + +0.1 297,679 + -1.8 297,679 + +0.3 297,679 + +0.3 297,679 + -1.8 297,679 + -1.2 297,679 + +2.2 297,680 - +0.2 297,800 - +1.2 297,947 + -0.5 297,970 + -1.0 297,970 + +0.3 297,988 + -0.0 298,011 - +0.8 298,011 - +1.2 298,140 - Pf1N1B4_239 0.11 -2.0 298,140 - Pf1N1B4_239 0.11 -1.5 298,140 - Pf1N1B4_239 0.11 -0.8 298,151 - Pf1N1B4_239 0.13 -0.4 298,251 - Pf1N1B4_239 0.24 -1.1 298,360 - Pf1N1B4_239 0.36 -0.6 298,372 - Pf1N1B4_239 0.37 -1.2 298,713 - Pf1N1B4_239 0.76 -0.8 298,713 - Pf1N1B4_239 0.76 -0.8 298,713 - Pf1N1B4_239 0.76 -0.8 298,736 + Pf1N1B4_239 0.78 -1.4 298,737 - +1.2 298,880 + -0.8 298,880 + -0.8 298,881 - -0.5 298,881 - -2.2 298,953 - +0.4 298,990 + +0.8 298,990 + -0.8 298,991 - -2.2 298,991 - +0.3 299,103 + Pf1N1B4_240 0.31 +1.4 299,103 + Pf1N1B4_240 0.31 -1.2 299,103 + Pf1N1B4_240 0.31 -2.0 299,189 - Pf1N1B4_240 0.58 -1.8 299,357 + -0.6 299,411 - -2.0 299,576 + Pf1N1B4_241 0.34 -0.4 299,587 + Pf1N1B4_241 0.39 +1.4 299,750 + -0.2 299,750 + +2.0 299,750 + +0.4 299,751 - +2.4 299,751 - -0.4 299,763 + -0.2 299,764 - -0.2 299,798 + +2.8 299,900 + +0.7 299,900 + -2.2 299,900 + +0.5 299,900 + +0.2 299,900 + +0.3 299,901 - -1.1 299,901 - +0.8 299,901 - +1.8
Or see this region's nucleotide sequence