Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2311 and Pf1N1B4_2312 are separated by 148 nucleotides Pf1N1B4_2312 and Pf1N1B4_2313 are separated by 115 nucleotides Pf1N1B4_2313 and Pf1N1B4_2314 are separated by 154 nucleotides Pf1N1B4_2314 and Pf1N1B4_2315 are separated by 28 nucleotides
Pf1N1B4_2311 - 4Fe-4S ferredoxin, iron-sulfur binding, at 2,631,681 to 2,631,932
_2311
Pf1N1B4_2312 - Phosphopantetheine adenylyltransferase (EC 2.7.7.3), at 2,632,081 to 2,632,560
_2312
Pf1N1B4_2313 - FIG022869: Oxidoreductase, GMC family, at 2,632,676 to 2,634,271
_2313
Pf1N1B4_2314 - FIG028932: hypothetical protein, at 2,634,426 to 2,634,971
_2314
Pf1N1B4_2315 - Aldehyde dehydrogenase (EC 1.2.1.3); Probable coniferyl aldehyde dehydrogenase (EC 1.2.1.68), at 2,635,000 to 2,636,430
_2315
Position (kb)
2632
2633
2634
2635 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2631.689 kb on + strand at 2631.707 kb on - strand, within Pf1N1B4_2311 at 2631.707 kb on - strand, within Pf1N1B4_2311 at 2631.936 kb on - strand at 2631.969 kb on - strand at 2632.056 kb on - strand at 2632.082 kb on + strand at 2632.082 kb on + strand at 2632.089 kb on + strand at 2632.649 kb on + strand at 2632.649 kb on + strand at 2632.650 kb on - strand at 2632.651 kb on + strand at 2632.651 kb on + strand at 2632.651 kb on + strand at 2632.651 kb on + strand at 2632.652 kb on - strand at 2632.652 kb on - strand at 2632.664 kb on + strand at 2632.665 kb on - strand at 2632.665 kb on - strand at 2632.902 kb on + strand, within Pf1N1B4_2313 at 2633.058 kb on + strand, within Pf1N1B4_2313 at 2633.073 kb on + strand, within Pf1N1B4_2313 at 2633.074 kb on - strand, within Pf1N1B4_2313 at 2633.269 kb on - strand, within Pf1N1B4_2313 at 2633.269 kb on - strand, within Pf1N1B4_2313 at 2633.316 kb on + strand, within Pf1N1B4_2313 at 2633.316 kb on + strand, within Pf1N1B4_2313 at 2633.316 kb on + strand, within Pf1N1B4_2313 at 2633.317 kb on - strand, within Pf1N1B4_2313 at 2633.317 kb on - strand at 2633.317 kb on - strand, within Pf1N1B4_2313 at 2633.317 kb on - strand, within Pf1N1B4_2313 at 2633.317 kb on - strand, within Pf1N1B4_2313 at 2633.317 kb on - strand, within Pf1N1B4_2313 at 2633.338 kb on - strand, within Pf1N1B4_2313 at 2633.381 kb on + strand, within Pf1N1B4_2313 at 2633.480 kb on + strand, within Pf1N1B4_2313 at 2633.480 kb on + strand, within Pf1N1B4_2313 at 2633.481 kb on - strand, within Pf1N1B4_2313 at 2633.481 kb on - strand, within Pf1N1B4_2313 at 2633.481 kb on - strand, within Pf1N1B4_2313 at 2633.484 kb on + strand, within Pf1N1B4_2313 at 2633.484 kb on + strand, within Pf1N1B4_2313 at 2633.484 kb on + strand, within Pf1N1B4_2313 at 2633.485 kb on - strand, within Pf1N1B4_2313 at 2633.503 kb on + strand, within Pf1N1B4_2313 at 2633.504 kb on - strand, within Pf1N1B4_2313 at 2633.607 kb on + strand, within Pf1N1B4_2313 at 2633.607 kb on + strand, within Pf1N1B4_2313 at 2633.607 kb on + strand, within Pf1N1B4_2313 at 2633.607 kb on + strand, within Pf1N1B4_2313 at 2633.986 kb on + strand, within Pf1N1B4_2313 at 2633.986 kb on + strand, within Pf1N1B4_2313 at 2633.986 kb on + strand, within Pf1N1B4_2313 at 2633.986 kb on + strand, within Pf1N1B4_2313 at 2633.986 kb on + strand, within Pf1N1B4_2313 at 2633.986 kb on + strand, within Pf1N1B4_2313 at 2633.987 kb on - strand, within Pf1N1B4_2313 at 2633.987 kb on - strand, within Pf1N1B4_2313 at 2633.987 kb on - strand, within Pf1N1B4_2313 at 2633.987 kb on - strand, within Pf1N1B4_2313 at 2633.987 kb on - strand, within Pf1N1B4_2313 at 2634.027 kb on + strand, within Pf1N1B4_2313 at 2634.027 kb on + strand, within Pf1N1B4_2313 at 2634.028 kb on - strand, within Pf1N1B4_2313 at 2634.039 kb on + strand, within Pf1N1B4_2313 at 2634.039 kb on + strand, within Pf1N1B4_2313 at 2634.040 kb on - strand, within Pf1N1B4_2313 at 2634.040 kb on - strand, within Pf1N1B4_2313 at 2634.040 kb on - strand, within Pf1N1B4_2313 at 2634.104 kb on + strand, within Pf1N1B4_2313 at 2634.104 kb on + strand, within Pf1N1B4_2313 at 2634.104 kb on + strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.111 kb on + strand, within Pf1N1B4_2313 at 2634.112 kb on - strand at 2634.112 kb on - strand at 2634.219 kb on + strand at 2634.220 kb on - strand at 2634.220 kb on - strand at 2634.220 kb on - strand at 2634.285 kb on + strand at 2634.285 kb on + strand at 2634.285 kb on + strand at 2634.288 kb on + strand at 2634.288 kb on + strand at 2634.288 kb on + strand at 2634.288 kb on + strand at 2634.289 kb on - strand at 2634.482 kb on - strand, within Pf1N1B4_2314 at 2634.845 kb on + strand, within Pf1N1B4_2314 at 2634.845 kb on + strand, within Pf1N1B4_2314 at 2634.846 kb on - strand, within Pf1N1B4_2314 at 2634.876 kb on + strand, within Pf1N1B4_2314 at 2634.920 kb on + strand at 2634.920 kb on + strand at 2634.921 kb on - strand at 2634.921 kb on - strand at 2634.921 kb on - strand at 2634.921 kb on - strand at 2634.972 kb on + strand at 2634.972 kb on + strand at 2634.973 kb on - strand at 2634.978 kb on + strand at 2634.979 kb on - strand at 2634.979 kb on - strand at 2635.042 kb on - strand at 2635.042 kb on - strand at 2635.055 kb on + strand at 2635.055 kb on + strand at 2635.055 kb on + strand at 2635.056 kb on - strand at 2635.139 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 2,631,689 + +0.4 2,631,707 - Pf1N1B4_2311 0.10 -0.2 2,631,707 - Pf1N1B4_2311 0.10 +0.8 2,631,936 - +0.6 2,631,969 - -0.5 2,632,056 - -1.2 2,632,082 + -0.1 2,632,082 + +2.7 2,632,089 + +1.7 2,632,649 + +0.4 2,632,649 + +3.0 2,632,650 - -0.4 2,632,651 + -0.8 2,632,651 + +1.4 2,632,651 + -0.1 2,632,651 + -0.0 2,632,652 - +0.7 2,632,652 - -1.0 2,632,664 + +0.3 2,632,665 - -1.8 2,632,665 - -0.6 2,632,902 + Pf1N1B4_2313 0.14 +1.4 2,633,058 + Pf1N1B4_2313 0.24 +1.4 2,633,073 + Pf1N1B4_2313 0.25 +1.4 2,633,074 - Pf1N1B4_2313 0.25 -0.3 2,633,269 - Pf1N1B4_2313 0.37 -0.8 2,633,269 - Pf1N1B4_2313 0.37 +0.2 2,633,316 + Pf1N1B4_2313 0.40 -0.3 2,633,316 + Pf1N1B4_2313 0.40 -0.9 2,633,316 + Pf1N1B4_2313 0.40 -0.7 2,633,317 - Pf1N1B4_2313 0.40 +1.4 2,633,317 - +2.4 2,633,317 - Pf1N1B4_2313 0.40 -1.8 2,633,317 - Pf1N1B4_2313 0.40 -0.6 2,633,317 - Pf1N1B4_2313 0.40 -1.6 2,633,317 - Pf1N1B4_2313 0.40 -0.2 2,633,338 - Pf1N1B4_2313 0.41 -1.8 2,633,381 + Pf1N1B4_2313 0.44 +0.7 2,633,480 + Pf1N1B4_2313 0.50 -0.6 2,633,480 + Pf1N1B4_2313 0.50 -1.2 2,633,481 - Pf1N1B4_2313 0.50 -1.4 2,633,481 - Pf1N1B4_2313 0.50 +0.4 2,633,481 - Pf1N1B4_2313 0.50 -0.0 2,633,484 + Pf1N1B4_2313 0.51 -0.9 2,633,484 + Pf1N1B4_2313 0.51 +0.8 2,633,484 + Pf1N1B4_2313 0.51 -0.2 2,633,485 - Pf1N1B4_2313 0.51 -1.4 2,633,503 + Pf1N1B4_2313 0.52 -0.9 2,633,504 - Pf1N1B4_2313 0.52 +2.4 2,633,607 + Pf1N1B4_2313 0.58 -0.5 2,633,607 + Pf1N1B4_2313 0.58 -1.2 2,633,607 + Pf1N1B4_2313 0.58 +0.8 2,633,607 + Pf1N1B4_2313 0.58 -0.9 2,633,986 + Pf1N1B4_2313 0.82 -0.2 2,633,986 + Pf1N1B4_2313 0.82 -1.1 2,633,986 + Pf1N1B4_2313 0.82 +1.4 2,633,986 + Pf1N1B4_2313 0.82 -1.0 2,633,986 + Pf1N1B4_2313 0.82 -0.7 2,633,986 + Pf1N1B4_2313 0.82 -1.0 2,633,987 - Pf1N1B4_2313 0.82 -0.6 2,633,987 - Pf1N1B4_2313 0.82 -0.5 2,633,987 - Pf1N1B4_2313 0.82 -1.4 2,633,987 - Pf1N1B4_2313 0.82 -0.1 2,633,987 - Pf1N1B4_2313 0.82 -0.2 2,634,027 + Pf1N1B4_2313 0.85 -1.0 2,634,027 + Pf1N1B4_2313 0.85 -0.6 2,634,028 - Pf1N1B4_2313 0.85 +0.2 2,634,039 + Pf1N1B4_2313 0.85 -1.6 2,634,039 + Pf1N1B4_2313 0.85 -0.6 2,634,040 - Pf1N1B4_2313 0.85 -0.9 2,634,040 - Pf1N1B4_2313 0.85 +0.7 2,634,040 - Pf1N1B4_2313 0.85 +0.1 2,634,104 + Pf1N1B4_2313 0.89 -1.3 2,634,104 + Pf1N1B4_2313 0.89 -0.4 2,634,104 + Pf1N1B4_2313 0.89 +0.1 2,634,105 - Pf1N1B4_2313 0.90 -0.6 2,634,105 - Pf1N1B4_2313 0.90 +1.4 2,634,105 - Pf1N1B4_2313 0.90 -0.2 2,634,105 - Pf1N1B4_2313 0.90 +0.5 2,634,105 - Pf1N1B4_2313 0.90 -0.4 2,634,105 - Pf1N1B4_2313 0.90 -0.9 2,634,105 - Pf1N1B4_2313 0.90 +1.2 2,634,105 - Pf1N1B4_2313 0.90 +0.6 2,634,111 + Pf1N1B4_2313 0.90 -1.4 2,634,112 - -0.6 2,634,112 - +0.1 2,634,219 + -0.6 2,634,220 - +0.8 2,634,220 - +0.1 2,634,220 - +1.6 2,634,285 + -0.6 2,634,285 + -0.3 2,634,285 + -0.1 2,634,288 + +0.7 2,634,288 + +0.2 2,634,288 + -0.2 2,634,288 + +0.6 2,634,289 - +0.9 2,634,482 - Pf1N1B4_2314 0.10 -0.2 2,634,845 + Pf1N1B4_2314 0.77 -1.4 2,634,845 + Pf1N1B4_2314 0.77 +0.7 2,634,846 - Pf1N1B4_2314 0.77 +0.4 2,634,876 + Pf1N1B4_2314 0.82 -0.5 2,634,920 + -0.2 2,634,920 + +1.0 2,634,921 - -0.9 2,634,921 - -1.0 2,634,921 - +0.6 2,634,921 - -0.9 2,634,972 + +2.7 2,634,972 + -0.2 2,634,973 - +1.4 2,634,978 + +0.5 2,634,979 - -0.0 2,634,979 - +1.0 2,635,042 - +0.9 2,635,042 - +0.3 2,635,055 + +1.4 2,635,055 + +0.4 2,635,055 + -0.9 2,635,056 - +0.2 2,635,139 + +0.4
Or see this region's nucleotide sequence