Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2207 and Pf1N1B4_2208 are separated by 18 nucleotides Pf1N1B4_2208 and Pf1N1B4_2209 are separated by 174 nucleotides
Pf1N1B4_2207 - Porphobilinogen synthase (EC 4.2.1.24), at 2,519,450 to 2,520,463
_2207
Pf1N1B4_2208 - Polyphosphate kinase (EC 2.7.4.1), at 2,520,482 to 2,522,704
_2208
Pf1N1B4_2209 - Exopolyphosphatase (EC 3.6.1.11), at 2,522,879 to 2,524,381
_2209
Position (kb)
2520
2521
2522
2523 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2519.544 kb on + strand at 2519.544 kb on + strand at 2519.544 kb on + strand at 2519.544 kb on + strand at 2519.545 kb on - strand at 2519.822 kb on - strand, within Pf1N1B4_2207 at 2519.848 kb on + strand, within Pf1N1B4_2207 at 2519.888 kb on + strand, within Pf1N1B4_2207 at 2519.888 kb on + strand, within Pf1N1B4_2207 at 2519.888 kb on + strand, within Pf1N1B4_2207 at 2519.888 kb on + strand, within Pf1N1B4_2207 at 2519.906 kb on - strand, within Pf1N1B4_2207 at 2519.924 kb on - strand, within Pf1N1B4_2207 at 2520.053 kb on + strand, within Pf1N1B4_2207 at 2520.054 kb on - strand, within Pf1N1B4_2207 at 2520.054 kb on - strand, within Pf1N1B4_2207 at 2520.065 kb on + strand, within Pf1N1B4_2207 at 2520.065 kb on + strand, within Pf1N1B4_2207 at 2520.065 kb on + strand, within Pf1N1B4_2207 at 2520.077 kb on + strand, within Pf1N1B4_2207 at 2520.077 kb on + strand, within Pf1N1B4_2207 at 2520.077 kb on + strand, within Pf1N1B4_2207 at 2520.078 kb on - strand, within Pf1N1B4_2207 at 2520.080 kb on + strand, within Pf1N1B4_2207 at 2520.143 kb on + strand, within Pf1N1B4_2207 at 2520.143 kb on + strand, within Pf1N1B4_2207 at 2520.143 kb on + strand, within Pf1N1B4_2207 at 2520.144 kb on - strand, within Pf1N1B4_2207 at 2520.242 kb on + strand, within Pf1N1B4_2207 at 2520.242 kb on + strand, within Pf1N1B4_2207 at 2520.277 kb on + strand, within Pf1N1B4_2207 at 2520.277 kb on + strand, within Pf1N1B4_2207 at 2520.277 kb on + strand, within Pf1N1B4_2207 at 2520.278 kb on - strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.296 kb on + strand, within Pf1N1B4_2207 at 2520.314 kb on + strand, within Pf1N1B4_2207 at 2520.314 kb on + strand, within Pf1N1B4_2207 at 2520.314 kb on + strand, within Pf1N1B4_2207 at 2520.314 kb on + strand, within Pf1N1B4_2207 at 2520.314 kb on + strand, within Pf1N1B4_2207 at 2520.388 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.419 kb on + strand at 2520.420 kb on - strand at 2520.420 kb on - strand at 2520.420 kb on - strand at 2520.461 kb on + strand at 2520.461 kb on + strand at 2520.650 kb on + strand at 2520.650 kb on + strand at 2520.651 kb on - strand at 2520.651 kb on - strand at 2520.651 kb on - strand at 2520.651 kb on - strand at 2521.232 kb on + strand, within Pf1N1B4_2208 at 2521.232 kb on + strand, within Pf1N1B4_2208 at 2521.232 kb on + strand, within Pf1N1B4_2208 at 2521.232 kb on + strand, within Pf1N1B4_2208 at 2521.232 kb on + strand, within Pf1N1B4_2208 at 2521.233 kb on - strand, within Pf1N1B4_2208 at 2521.233 kb on - strand, within Pf1N1B4_2208 at 2521.460 kb on + strand, within Pf1N1B4_2208 at 2521.499 kb on + strand, within Pf1N1B4_2208 at 2521.499 kb on + strand, within Pf1N1B4_2208 at 2521.500 kb on - strand, within Pf1N1B4_2208 at 2521.574 kb on + strand, within Pf1N1B4_2208 at 2521.574 kb on + strand at 2521.575 kb on - strand, within Pf1N1B4_2208 at 2521.575 kb on - strand, within Pf1N1B4_2208 at 2521.751 kb on + strand, within Pf1N1B4_2208 at 2521.752 kb on - strand, within Pf1N1B4_2208 at 2521.792 kb on - strand, within Pf1N1B4_2208 at 2521.910 kb on + strand, within Pf1N1B4_2208 at 2522.010 kb on - strand, within Pf1N1B4_2208 at 2522.042 kb on + strand, within Pf1N1B4_2208 at 2522.043 kb on - strand, within Pf1N1B4_2208 at 2522.279 kb on + strand, within Pf1N1B4_2208 at 2522.280 kb on - strand, within Pf1N1B4_2208 at 2522.280 kb on - strand, within Pf1N1B4_2208 at 2522.420 kb on + strand, within Pf1N1B4_2208 at 2522.421 kb on - strand, within Pf1N1B4_2208 at 2522.561 kb on + strand at 2522.660 kb on - strand at 2522.707 kb on + strand at 2522.707 kb on + strand at 2522.707 kb on + strand at 2522.707 kb on + strand at 2522.707 kb on + strand at 2522.708 kb on - strand at 2523.011 kb on + strand at 2523.011 kb on + strand at 2523.012 kb on - strand at 2523.012 kb on - strand at 2523.012 kb on - strand at 2523.012 kb on - strand at 2523.012 kb on - strand at 2523.225 kb on + strand, within Pf1N1B4_2209 at 2523.226 kb on - strand, within Pf1N1B4_2209 at 2523.226 kb on - strand, within Pf1N1B4_2209 at 2523.243 kb on + strand, within Pf1N1B4_2209 at 2523.244 kb on - strand, within Pf1N1B4_2209 at 2523.244 kb on - strand, within Pf1N1B4_2209 at 2523.249 kb on + strand, within Pf1N1B4_2209 at 2523.249 kb on + strand, within Pf1N1B4_2209 at 2523.250 kb on - strand, within Pf1N1B4_2209 at 2523.250 kb on - strand, within Pf1N1B4_2209 at 2523.250 kb on - strand, within Pf1N1B4_2209 at 2523.474 kb on + strand, within Pf1N1B4_2209 at 2523.474 kb on + strand, within Pf1N1B4_2209 at 2523.475 kb on - strand, within Pf1N1B4_2209 at 2523.475 kb on - strand, within Pf1N1B4_2209
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 2,519,544 + -0.9 2,519,544 + +1.5 2,519,544 + -0.9 2,519,544 + -0.9 2,519,545 - +1.0 2,519,822 - Pf1N1B4_2207 0.37 -0.5 2,519,848 + Pf1N1B4_2207 0.39 +1.7 2,519,888 + Pf1N1B4_2207 0.43 -0.2 2,519,888 + Pf1N1B4_2207 0.43 -0.3 2,519,888 + Pf1N1B4_2207 0.43 +0.4 2,519,888 + Pf1N1B4_2207 0.43 -0.1 2,519,906 - Pf1N1B4_2207 0.45 -0.2 2,519,924 - Pf1N1B4_2207 0.47 -0.1 2,520,053 + Pf1N1B4_2207 0.59 +1.9 2,520,054 - Pf1N1B4_2207 0.60 -0.5 2,520,054 - Pf1N1B4_2207 0.60 -0.9 2,520,065 + Pf1N1B4_2207 0.61 -0.3 2,520,065 + Pf1N1B4_2207 0.61 +0.7 2,520,065 + Pf1N1B4_2207 0.61 -0.4 2,520,077 + Pf1N1B4_2207 0.62 +1.8 2,520,077 + Pf1N1B4_2207 0.62 -0.5 2,520,077 + Pf1N1B4_2207 0.62 +0.5 2,520,078 - Pf1N1B4_2207 0.62 -0.1 2,520,080 + Pf1N1B4_2207 0.62 +0.1 2,520,143 + Pf1N1B4_2207 0.68 +1.5 2,520,143 + Pf1N1B4_2207 0.68 +2.1 2,520,143 + Pf1N1B4_2207 0.68 -0.1 2,520,144 - Pf1N1B4_2207 0.68 +0.0 2,520,242 + Pf1N1B4_2207 0.78 -1.3 2,520,242 + Pf1N1B4_2207 0.78 +1.1 2,520,277 + Pf1N1B4_2207 0.82 +0.7 2,520,277 + Pf1N1B4_2207 0.82 -0.5 2,520,277 + Pf1N1B4_2207 0.82 +0.5 2,520,278 - Pf1N1B4_2207 0.82 -0.1 2,520,296 + Pf1N1B4_2207 0.83 +0.4 2,520,296 + Pf1N1B4_2207 0.83 +1.5 2,520,296 + Pf1N1B4_2207 0.83 +0.7 2,520,296 + Pf1N1B4_2207 0.83 +0.9 2,520,296 + Pf1N1B4_2207 0.83 -1.1 2,520,296 + Pf1N1B4_2207 0.83 +0.9 2,520,296 + Pf1N1B4_2207 0.83 -0.0 2,520,314 + Pf1N1B4_2207 0.85 +0.1 2,520,314 + Pf1N1B4_2207 0.85 +0.9 2,520,314 + Pf1N1B4_2207 0.85 +0.3 2,520,314 + Pf1N1B4_2207 0.85 -0.9 2,520,314 + Pf1N1B4_2207 0.85 -0.3 2,520,388 + -0.2 2,520,419 + +0.8 2,520,419 + +0.9 2,520,419 + -0.5 2,520,419 + -0.4 2,520,419 + +2.5 2,520,419 + +2.1 2,520,419 + +0.1 2,520,419 + +1.1 2,520,420 - -0.5 2,520,420 - -0.5 2,520,420 - -0.5 2,520,461 + +1.1 2,520,461 + -1.3 2,520,650 + -0.1 2,520,650 + +0.7 2,520,651 - -1.3 2,520,651 - -2.0 2,520,651 - +1.2 2,520,651 - -1.5 2,521,232 + Pf1N1B4_2208 0.34 -1.5 2,521,232 + Pf1N1B4_2208 0.34 -1.3 2,521,232 + Pf1N1B4_2208 0.34 -1.5 2,521,232 + Pf1N1B4_2208 0.34 -1.5 2,521,232 + Pf1N1B4_2208 0.34 -0.5 2,521,233 - Pf1N1B4_2208 0.34 -1.5 2,521,233 - Pf1N1B4_2208 0.34 -0.1 2,521,460 + Pf1N1B4_2208 0.44 -0.7 2,521,499 + Pf1N1B4_2208 0.46 -1.1 2,521,499 + Pf1N1B4_2208 0.46 -2.6 2,521,500 - Pf1N1B4_2208 0.46 -2.2 2,521,574 + Pf1N1B4_2208 0.49 -0.9 2,521,574 + -0.1 2,521,575 - Pf1N1B4_2208 0.49 -2.3 2,521,575 - Pf1N1B4_2208 0.49 +0.5 2,521,751 + Pf1N1B4_2208 0.57 -0.1 2,521,752 - Pf1N1B4_2208 0.57 +1.1 2,521,792 - Pf1N1B4_2208 0.59 -1.3 2,521,910 + Pf1N1B4_2208 0.64 -1.6 2,522,010 - Pf1N1B4_2208 0.69 -1.0 2,522,042 + Pf1N1B4_2208 0.70 -1.1 2,522,043 - Pf1N1B4_2208 0.70 +0.6 2,522,279 + Pf1N1B4_2208 0.81 -2.4 2,522,280 - Pf1N1B4_2208 0.81 +0.5 2,522,280 - Pf1N1B4_2208 0.81 -2.4 2,522,420 + Pf1N1B4_2208 0.87 +0.5 2,522,421 - Pf1N1B4_2208 0.87 +0.1 2,522,561 + -0.9 2,522,660 - -0.1 2,522,707 + -0.1 2,522,707 + -0.3 2,522,707 + -0.4 2,522,707 + +0.6 2,522,707 + -2.1 2,522,708 - -1.1 2,523,011 + +0.3 2,523,011 + +1.1 2,523,012 - +0.3 2,523,012 - +1.9 2,523,012 - +2.2 2,523,012 - +0.3 2,523,012 - +0.5 2,523,225 + Pf1N1B4_2209 0.23 +1.2 2,523,226 - Pf1N1B4_2209 0.23 +0.2 2,523,226 - Pf1N1B4_2209 0.23 +0.8 2,523,243 + Pf1N1B4_2209 0.24 +1.0 2,523,244 - Pf1N1B4_2209 0.24 -0.5 2,523,244 - Pf1N1B4_2209 0.24 +1.5 2,523,249 + Pf1N1B4_2209 0.25 +0.3 2,523,249 + Pf1N1B4_2209 0.25 +0.8 2,523,250 - Pf1N1B4_2209 0.25 +0.9 2,523,250 - Pf1N1B4_2209 0.25 +2.2 2,523,250 - Pf1N1B4_2209 0.25 +1.5 2,523,474 + Pf1N1B4_2209 0.40 -0.5 2,523,474 + Pf1N1B4_2209 0.40 +0.1 2,523,475 - Pf1N1B4_2209 0.40 -0.9 2,523,475 - Pf1N1B4_2209 0.40 +0.5
Or see this region's nucleotide sequence